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고등식물의 생장에 미치는 납의 영향

  • Park, Jong-Beom
    • Proceedings of the Korean Environmental Sciences Society Conference
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    • 2007.05a
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    • pp.465-467
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    • 2007
  • 애기장대에 납을 농도별로 처리하였을 때 식물의 생장과 종자 발아에 미치는 영향을 조사하였다. 환경부고시 오염물질 배출기준치를 기준 농도로 하고 이보다 10배 및 50배 높은 농도의 납을 각각 처리한 애기장대의 줄기생장을 조사한 결과 오염물질 배출기준 농도와 이보다 10배 높은 농도에서는 줄기 생장이 정상식물보다 약 18%정도 감소되었다. 또한 오염물질 배출기준 농도보다 50배 높은 농도를 처리한 식물에서는 정상식물보다 약 41% 줄기생장이 감소하였다. 3가지 농도의 납을 애기장대에 처리한 후 식물의 뿌리 생장을 조사한 결과 오염물질 배출기준농도를 처리한 식물의 뿌리 생장은 정상식물과 거의 유사하였으나 오염물질 배출기준농도의 10배 이상이 되면 뿌리 생장이 전혀 되지 않았다. 3가지 농도의 납을 처리한 배지에서 애기장대의 종자 발아율을 측정한 결과 오염물질 배출기준 농도와 이보다 10배 높은 농도를 처리한 배지에서는 종자 발아율이 100%로 나타나 정상배지에서의 발아율과 차이가 나타나지 않았으나 오염물질 배출기준 농도보다 50배 높은 농도를 처리한 배지에서는 발아율이 0%로 나타나 종자 발아가 전혀 되지 않았다. 이러한 결과는 토양 속에 침적된 납은 애기장대의 생장과 종자 발아에 영향을 미치어 줄기 및 뿌리의 생장을 감소시켰으며, 또한 종자 발아에도 매우 치명적인 영향을 미치는 것으로 생각된다.

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Mass-production of Eleutherococcus seoulensis Seedlings Through Somatic Embryogenesis (체세포배 형성을 통한 서울오갈피(Eleutherococcus seoulensis) 묘목의 대량생산)

  • Lee, Su-Gwang;Kang, Ho-Duck
    • Journal of Korean Society of Forest Science
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    • v.98 no.6
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    • pp.719-725
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    • 2009
  • This study was conducted to establish the optimal condition for acclimatization from somatic embryos of Eleutherococcus seoulensis. Torpedo-shaped embryos of Eleutherococcus seoulensis were cultured on 1/3 MS and WPM media supplemented with $GA_3$ (3.0, 5.0 mg/L) for 4 weeks. Plentlets were transferred to 1/2 SH solid medium with 1.0 mg/L $GA_3$ and 0.2% activated charcoal for shoot and root elongation and them elongated plantlets further developed on 1/2 SH medium for 4 weeks. Developed plantlets further elongated into well-shaped leaf and root system on 1/3 SH medium under ventilation condition for 4 weeks. Plantlets grew normally on 1/3 SH basal medium, were acclimated on various soil. Survival frequency of plantlets was influenced by soil type(peatmoss+perlite, perlite, soil on Nam mountain). The highest survival rate to soil was more than 70% when plantlets were 1/3 SH medium under ventilation condition in Nam mountain soil. These results indicate that the systematic procedure of plant production in Eleutherococcus seoulensis could be practically applicable for mass propagation.

Characterization of Stretchable Polypyrrole Films Prepared by Chemical and Electrochemical Method (화학적 및 전기화학적 방법으로 합성한 연신성 폴리피롤 필름의 특성)

  • 장관식;문봉진;오응주;홍장후
    • Polymer(Korea)
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    • v.27 no.4
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    • pp.323-329
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    • 2003
  • Stretchable Polypyrrole films using functionalized doping agent, di(2-ethylhexyl) sulfosuccinate sodium salt (NaDEHS), were synthesized by chemical and electrochemical method. Chemically and electrochemically Prepared Polypyrrole films were stretch-oriented (L/L$\_$0/= 1.0 ∼ 2.5) by zone drawing method and the electrical conductivities were measured. As the draw ratio was increased, the electrical conductivities were increased. This result was confirmed by the increase in crystallinity through the increase in draw ratio. The temperature dependence of electrical conductivity showed that 3D-variable range hopping model (L/L$\_$0/ = 1.0∼2.0) and ID-VRH model (L/L$\_$0/ = 2.5) gave the best fit to the data for stretched Ppy-DEHS films.

The Relations between metabolic syndrome, physical activity, and dietary patterns in Korean adults (한국 성인의 대사증후군과 신체활동, 식이 형태의 관계)

  • Bang, So-Youn
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.20 no.2
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    • pp.662-672
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    • 2019
  • This study involved a secondary analysis of 2016 Korean National Health and Nutrition Examination Survey data to identify the relations between metabolic syndrome, physical activity, and dietary patterns in Korean adults. The ${\chi}^2$-test, t-test, and logistic regression analysis of complex samples were conducted on the data of 3,352 subjects aged 19 to 64 years with metabolic syndrome. The prevalences of metabolic syndrome among men and women were 29.7% and 16.7%, respectively. In men, the high physical activity group showed a 0.8-fold increase in the likelihood of metabolic syndrome (OR=0.8, p=.038), and higher fat intake increased the risk of metabolic syndrome 0.98-fold (OR=0.98, p=.007). In women, the high physical activity group showed a 0.7-fold increase in the likelihood of metabolic syndrome (OR=0.71, p=.034), higher carbohydrate intake increased the risk of metabolic syndrome by 1.03-fold (OR=1.03, p<.001), and that higher protein and fat intakes reduced the risk by 0.96-fold (OR=0.96, p=.009) and 0.95-fold (OR=0.95, p<.001), respectively. The study shows that to meet the challenge presented by the increasing prevalence of metabolic syndrome, efforts are needed to screen for adults at risk of the metabolic syndrome by regular physical examination and to develop and provide intervention programs that involve regular high intensity physical activity and controlled carbohydrate, protein, and fat intakes.

Observations on the Modulated Structure in Pyrochlore-type Compounds, $In_2(Ti_{1.7}Zn_{0.3})O_{0.67}$ and $In_2(Ti_{1.7}Mg_{0.3})O_{6.7}$ (Pyrochlore형 화합물 $In_2(Ti_{1.7}Zn_{0.3})O_{0.67}$$In_2(Ti_{1.7}Mg_{0.3})O_{6.7}$에서의 변조구조 관찰)

  • Lee, Hwack-Joo;Park, Hyun-Min;Cho, Yang-Koo;Ryu, Hyun;Nahm, Sahn;Bando, Y.
    • Applied Microscopy
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    • v.29 no.4
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    • pp.471-477
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    • 1999
  • Microstructural observations on the pyrochlore-type $Lu_2Ti_2O_7$ and the similar type of compounds, $In_2(Ti_{1.7}Zn_{0.3})O_{6.7}$ and $In_2(Ti_{1.7}Mg_{0.3})O_{6.7}$ which were made by the isothermal heat-treatment at 1623K for 18 days in Pt tube, were carried out using a top-entry HRTEM working at 200 kV. The modulated structures were found in both compounds, however, not in $Lu_2Ti_2O_7$. From the electron diffraction pattern analysis, the modulated superlattices are incommensurate and are 2.69 times of sublattices along (220) direction. The high resolution TEM images have shown that the superlattices consist of alternate superlattices which are composed of two or three sublattices, resulting in the average of 2.7 times of sublattices in accordance with the analysis of electron diffraction patterns. The crystal structures of both compounds are found to quite similar to those of pyrochlore, however the evidence that the cubic axes are slightly deviated from right angle. The modulated structure has gradually changed to the unmodulated structure induced by electron irradiation.

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Antioxidant, Tyrosinase Inhibitory, and Anti-proliferative Activities of Gochujang Added with Cheonggukjang Powder Made from Sword Bean (작두콩 청국장 첨가 고추장의 항산화, tyrosinase 저해 및 암세포 증식 억제 효과)

  • Chang, Moon-Ik;Kim, Jae-Young;Kim, Un-Sung;Baek, Seung-Hwa
    • Korean Journal of Food Science and Technology
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    • v.45 no.2
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    • pp.221-226
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    • 2013
  • This study aimed to examine the antioxidant, tyrosinase inhibitory, and anti-proliferative activities (A549, G361, HT-29, and MDA-MB-231) of fermented gochujang (made from sword bean cheonggukjang powder (SBC) for 90 days. Gochujang was prepared by adding 0 (SBC 0), 2 (SBC 2), 5 (SBC 5), 8 (SBC 8) and 10% (SBC 10) levels with SBC, and all experiments were measured at diluted levels of 20, 50 and 100 times. The antioxidant activity and tyrosinase inhibitory effect demonstrated that SBC 10 increased approximately 1.2 and 1.1 times compared with SBC 0, respectively, at diluted levels of 50 and 100 times. The anti-proliferative effects of A549, G361, and HT-29 presented that SBC 10 were 2.8, 1.1, and 8.9 times higher compared with SBC 0, respectively, at diluted levels of 50, 20, and 100 times. In the case of MDA-MB-231, SBC 10 was 3.7 times higher compared with SBC 5 at diluted level of 20 times. As a result, we confirmed that SBC gochujang was improved for physiological activities and anti-proliferative effects.

Effect of Cryoprotectant Concentration and Equilibration Time on Volume Change and In Vitro Development of Intact and Bisected Mouse Embryos following Rapid Freezing (동결보호제의 농도와 평형시간이 생쥐의 정상배 및 분할배의 용적 변화와 체외 발달에 미치는 영향)

  • 이은봉;공일근;강대진;박충생
    • Korean Journal of Animal Reproduction
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    • v.16 no.1
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    • pp.47-53
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    • 1992
  • This study was carried out to investage the effect of cryoprotectant concentration and equilibration time on volume change and in vitro development of intact and bisected mouse embryos by rapid freezing. When compacted morulae were rapidly frozen in 3.0 to 4.0 glycerol or DMSO with 0.25M sucrose solution, the superior(P<0.05) post-thaw survival rate was obtained at the glycerol concentration of 4.0M(89.4%) than 3.0M(71.4%) or 5.0M(42.4%), but at the DMSO concentration of 3.0M(84.5%) than 4.0M(51.1%) or 5.0M(0.0%). The optimal equilibraton time for rapid freezing of ZP-free or bisected morulae in 4.0M glycerol with 0.25M sucrose was found tobe 3 minutes. The minimal volume of compacted morulaewhich corresponded with 61 to 62% of pre-equilibrated embryo volume was obtained from equilibration for 3 minutes in both 3.0 and 4.0M glycerol solutions with 0.25M sucrose.

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Multicotyledonary Structure of Somatic Embryos Formed from Cell Cultures of Daucus carota L. (당근의 배양세포로부터 형성된 체세포배의 다자엽 구조)

  • 소웅영
    • Journal of Plant Biology
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    • v.39 no.1
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    • pp.71-77
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    • 1996
  • Embryogenic callus was selected from callus induced from hypocotyl segment cultures of Daucus carota seedlings on MS basal medium supplemented with 1.0 mg/L 2,4-D. Cell clumps prepared from the embryogenic callus were transferred to MS medium without 2,4-D for somatic embryo development. Cotyledonary abnormalities were frequently observed on somatic embryos developed after the incubation of cell clumps in MS basal medium with 1.0 mg/L 2,4-D for one week and then subculture in the same medium but without 2.4-D for two weeks. The percentage of abnormalities was as follows: 5% one cotyledon, 21% three cotyledons, 6% four cotyledons, 5% five cotyledons, 0.2% six cotyledons and 1% trumpet-like cotyledons. On the other hand, the normal somatic embryo with two cotyledons appeared at 63%. The germination rate of somatic embryos was higher in two cotyledon somatic embryos than in multicotyledonary embryos. Trumpet-like somatic embryos did not germinate normally showing limited elongation and enlargement of roots and cotyledons without shoot development. From anatomical examination circular procambium in the root of somatic embryo began to branch around the middle regions of the hypocotyl which extended into the cotyledons through the cotyledonary nodes and the number of branched procambial strands in hypocotyl was equal to the number of cotyledons. Monocotyledonous somatic embryo always had larger cotyledon than that of somatic embryos with multicotyledons and had horseshoe-like cotyledons where the procambium was of the same structure.ucture.

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Studies on the Germination of Korean Ginseng (Panax ginseng C.A.Meyer) Seed II. Influences of Temperature and Seed Treatment on Embryo Growth and Germination (인삼 종자의 발아에 관한 연구 II. 온도 및 종자처리가 배생장 및 발아에 미치는 영향)

  • 원준연;조재성;김현호
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.33 no.1
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    • pp.59-63
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    • 1988
  • Freshly harvested and depulped Korean ginseng seeds were subjected to the seed treatment of removing endocarp plus surface sterilization with sodium hypocloride, surface sterilization only, and nontreated control. These seeds were stratified at temperatures of 5$^{\circ}$, 10$^{\circ}$, 15$^{\circ}$ and 20$^{\circ}C$ for 20, 40, 60, 80 and 100 days. Embryo growth of the ginseng seeds of which endocarp was removed was most rapid in each stratification temperature and that of sterilized seeds was slower than unsterilized seeds after 80 days stratification at 15$^{\circ}$ and 20$^{\circ}C$. About 15$^{\circ}C$ was an optimal stratification temperature for embry growth in ginseng seeds. Chilling treatment at 5$^{\circ}C$ for 100 days was needed for better germination of dehisced ginseng seeds. An optimal germination temperature for the ginseng seed following chilling treatment was about 15$^{\circ}C$.

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In Vitro Studies on Pinus koraiensis (I) - Establishment and Growth of Callus - (잣나무의 기내배양(器內培養)에 관(關)한 연구(硏究) (I) - Callus 의 유발(誘發)과 생장(生長) -)

  • Park, Young Goo;Kim, Oue Ryong
    • Journal of Korean Society of Forest Science
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    • v.59 no.1
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    • pp.51-56
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    • 1983
  • Embryo tissue from Pinus koraiensis was established in culture on G.D. and W.S. basal medium supplemented with the auxin (NAA, 2,4-D, IBA) and kinetin. The various combinations of growth regulators were studied in order to determine the specific requirements of the callus tissue in vitro. The inorganic nutrient combination of G.D. medium was found to be better than that of W,S. medium. G,D. basal medium supplemented with 0.1 ppm NAA and 0.1 ppm kinetin was the most successful nutrient combination for the growth of callus induced from the embryo of P. koraiensis.

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