• Korean Journal of Plant Resources
• /
• v.11 no.1
• /
• pp.9-14
• /
• 1998

Two types of somatic embryos were directly induced from the immature zygotic embryos of the wild prunus yedoensis in Mt. Halla after 16 weeks of culture on MS medium supplemented with 0.1mg/L $GA_3$ and 0.1mg/L BAPor 0.5mg/L $GA_3$ and 0.1mg/L BAP. One was normal single embryo with a single basal part. Normal somatic embryos germinated successfully on 1/2 MS medium. However, abnormal nulticotyledonary somatic embryos, formed shoots only on hormone free MS medium and about 80% of shoots rooted on MS medium with 0.5mg/L IBA. The mximum frequency (62.5%) of normal somatic embryos was directly obtained from the zygotic embryo 30 days after full blooming but it was decreased with further maturation.

• Horticultural Science & Technology
• /
• v.32 no.1
• /
• pp.100-104
• /
• 2014

This study was conducted to determine the optimal MS medium strength to improve sprouting rate of apical meristem of strawberry 'Seolhyang' in vitro. Strawberry apical meristems at size (0.2 mm to 0.3 mm) with leaf primordials were cultured on the MS media with four strength levels, ($1/4{\times}$, $1/3{\times}$, $1/2{\times}$, and $1{\times}$) and the sprouting rate and growth characteristics were evaluated after eight weeks after cultivation. Shoot rate of 'Daewang' apical meristems was 93.6%whereas 'Seolhyang' apical meristems were sprouted with 31.6% on $1{\times}$ MS medium strength. Different sprouting rates were observed in 'Seolhyang' apical meristem with 31.6% in $1{\times}$ medium, 75.0% in $1/2{\times}$ medium, and 94.4% in $1/3{\times}$ medium. The sprouting rate was improved with the decrease of medium strength, but the shoot rate in $1/4{\times}$ medium decreased up to 54.5%. Shoot length was 0.9 cm in $1{\times}$ medium, 1.2 cm in $1/2{\times}$ medium, 1.6 cm in $1/3{\times}$ medium, and 1.9 cm in $1/4{\times}$ medium. Shoot length was longer as medium strength decreased and numbers of leaves and roots were not significant differences among the medium strengths. As a result, sprouting rate was highest and plant growth was best in $1/3{\times}$ MS medium compared to the others.

• Korean Journal of Plant Tissue Culture
• /
• v.25 no.1
• /
• pp.1-5
• /
• 1998

This study describes the effect of the growth regulators such as 2,L-D and BA, on the structural abnormalities of somatic embryos derived from leaf explants of Angelica gigas Nakai, Also, the relationship between the cotyledon number of a somatic embryo and its germinability is explored. Embryogenic calli were selected from calli formed on explants cultured on MS solid basal medium supplemented with 0.5mg/L 2,4-D, 1mg/L 2,4-D, 1mg/L plus 0.1mg/L BA, and 1 mg/L 2,4-D plus 0.5mg/L BA. Cotyledonary abnormalities were observed in somatic embryos which were developed from embryogenic calli cultured on MS medium containing 1mg/L 2,4-D for 8 weeks and then subcultured on 2,4-D free MS medium for 3 weeks. The frequency of abnormalities was as follows: 22.8% one cotyledon, 42.5% two cotyledons, 16.8% three cotyledons, 7.8% four cotyledons, 1.8% five cotyledons, and 8.2% jar shaped cotyledon. In addition, ABA treatment indicated an improvement of the somatic embryo with normal cotyledon (65.3%). ABA was important role to the high production of normal somatic embryos. Two cotyledon embryos showed germinability 77.8%. However the germinability of somatic embryos with anomalous cotyledons was prominently low: One cotyledon, 62.5%; three cotyledons, 43.3%; four cotyledons, 60%; five cotyledons, 50% and jar shaped cotyledon, zero%. Thus, germinability was essentially, inversely proportional to cotyledon number.

• Journal of Plant Biotechnology
• /
• v.39 no.2
• /
• pp.93-98
• /
• 2012

This study was conducted to evaluate effects of somatic embryos (SEs) induction with different kinds and concentrations of osmoticum, with gelrite and SEs germination with different abscisic acid (ABA) and embryogenic tissue lines (ETLs) in Japanese red pine (Pinus densiflora). In comparison of somatic embryos induction with different kinds and concentrations of osmoticum, the highest record (45/90 mg FW) was obtained from the treatment of 0.1 M maltose+3.75% PEG 4000. In addition, the higher one was also recorded from 0.2 M maltose (41 SEs), it turned out this treatment was also effective in induction of SEs with previous one. In effects of various gelrite concentrations for SEs production, no SEs were occurred in the treatment of 0.4 or 0.6% gelrite, however, the highest no. of SEs shown in 1.0% gellrite (41 SEs), and some comparable results were also marked with 0.8 (37.3 SEs) or 1.2% (39.7 SEs) gelrite. Therefore, SEs can be produced from above 0.8% concentration of gelrite. Finally, in comparison of SEs germination with different concentrations of ABA, ETLs, and AC, the best germination rates (45%) were obtained from the SEs derived from both concentrations of 150 and $200{\mu}M$ ABA in 06-6 ETL and when cultured on germination supplemented with 0.2% AC. When SEs were cultured on germination medium without AC, best germination rate (28.9%) came from the SEs which cultured on $250{\mu}M$ ABA in 06-6 ETL.

• Proceedings of the KSAR Conference
• /
• 2001.03a
• /
• pp.32-32
• /
• 2001

본 연구는 돼지 난포란의 체외성숙시 Adenylate cyclase(ACi) 첨가가 돼지 수정란의 배발달에 미치는 효과를 검토하기 위하여 수행하였다. 돼지 난포란은 0.1% PVA, 3.05 mM D-Glucose, 0.91 mM sodium pyruvate, 0.57 mM cysteine, 0.5 $\mu\textrm{g}$/$m\ell$ LH, 0.5 $\mu\textrm{g}$/$m\ell$ FSH 그리고 10 ng/$m\ell$ EGF가 첨가된 TCM-199 배양액에서 ACi를 농도별로 첨가하여 42~44 시간 배양함으로써 체외성숙을 유도하였다. 그리고 ACi가 배발달율에 미치는 효과를 알아보기 위하여 체외수정 medium에 0, 0.1, 1% 첨가한 다음, 체외수정을 유도하고 체외배양을 실시하였다 체외성숙시 22시간 동안 ACi를 0, 0.1, 1, 및 10 $\mu\textrm{g}$/$m\ell$로 처리한 결과 33.3, 31.5, 34.1, 및 36.0%로 대조구와 처리구간의 통계학적인 유의한 차이는 나타나지 않았다. 그러나 체외성숙시 ACi을 0, 0.01, 0.1, 1.0 $\mu\textrm{g}$/$m\ell$로 처리한 결과, 25.5%, 33.3, 26.7, 및 25.6%의 배발달율이 확인되어 유의한 ACi의 처리효과가 확인되었다. 이러한 처리효과는 ACi를 0.01%로 첨한 처리구(배발달율, 33.3%)에서 가장 높게 나타났다. 그리고 체외수정시 caffeine과 ACi를 첨가한 실험에서, 1 mM caffeine과 ACi를 0, 1, 10, 20 $\mu\textrm{g}$/$m\ell$로 첨가한 결과, 대조구(30.4%)에 비해 처리구(40.9, 37.9, 및 43.5%)에서 높은 배발달율을 나타냈다. 0.5 mM caffeine과 ACi를 0, 1, 10, 20 $\mu\textrm{g}$/$m\ell$ 로 첨가시는 대조구(37.8%)와 처리구(36.5, 36.0, 및 36.0%)간의 유의한 차이는 나타나지 않았다. 또한 ACi가 배발달율에 미치는 효과를 알아보기 위하여 체외수정 배양액에 0, 0.1, 1% 첨가한 결과는 Table 1에서 보는 바와 같이, 처리구(30.3와 37.0%)가 대조구(22.6%)보다 통계학적으로 유의하게 높은 배발달율을 나타냈다. 따라서 본 연구의 결과는 체외성숙 또는 체외수정용 배양액 내에 ACi을 첨가함으로써 초기배의 발달을 효과적으로 유도할 수 있을 것이며, 앞으로 이러한 기술을 활용한다면 좀더 효율적으로 형질전환 동물이나 복제동물을 생산하게 될 것으로 사료된다.

• Korean Journal of Microbiology
• /
• v.50 no.1
• /
• pp.60-66
• /
• 2014

This study was carried out to investigate the characteristics of FOST (fermented omija sugar treatment extracts) using Lactobacillus brevis HLJ59. Antioxidant activities of FOST were evaluated through viable cell number of L. brevis HLJ59, DPPH radical scavenging activity, reducing power and SOD-like activity, compared to non-FOST(non-fermented omija sugar treatment extracts). Also it was to evaluate Angiotensin Converting Enzyme (ACE) and Urease inhibitory activity of FOST. The viable cell number of L. brevis was about $2.05{\pm}0.21{\times}10^8$, $6.31{\pm}0.56{\times}10^{11}$, and $8.14{\pm}0.14{\times}10^9$ at ${\times}2$, ${\times}5$, and ${\times}10$ dilution, respectively. DPPH radical scavenging activity of FOST was about 60.3%, 71.8%, and 44.5% at ${\times}2$, ${\times}5$, and ${\times}10$ dilution, respectively. The reducing power of FOST was about 0.92, 1.19, and 0.73 (OD at 700 nm) at ${\times}2$, ${\times}5$, and ${\times}10$ dilution, and SOD-like activity of FOST was about 50.4%, 53.7%, and 33.4% at ${\times}2$, ${\times}5$, and ${\times}10$ dilution, respectively. ACE and Urease inhibitory activity by FOST was about 47.4%, 78.2%, 56.4% and 58.1%, 83.4%, 63.2% at ${\times}2$, ${\times}5$, and ${\times}10$ dilution, respectively. The results indicated that the fermentation of omija sugar treatment extracts using Lactobacillus brevis HLJ59 increased the antioxidant activities campared to the non-fermented omija sugar treatment extracts.

• Journal of Bio-Environment Control
• /
• v.12 no.1
• /
• pp.22-25
• /
• 2003

Seedlings of ‘Nokgwang’ and ‘Kwari’ green pepper were cultured in 32-cell plug trays in TK$_2$ medium to find out optimal concentrations of phosphate. Seedling growth of both ‘Nokgwang’ and ‘Kwari’ cultivars was affected by phosphate strength. Applying P fertilizer to the plug system caused a marked increase in plant height and leaf area at 1.0 phosphate strength. On the other hand, total dry weight increased with increasing phosphate strength. Higher chlorophyll content was observed in ‘Nokgwang’ than ‘Kwari’ in all treatments. Phosphate strength greater than 0.5 gave similar chlorophyll content. Photosynthetic rate was higher for plants fertilized with 1.0 strength for ‘Nokgwang’ and 0.5 strength for ‘Kwari’than the other treatments. Higher concentrations of phosphate reduced photosynthesis in both cultivars. With ‘Nokgwang’increasing concentration of phosphate up to 1.0 strength resulted in increase in stomatal conductance and transpiration rate.

• Journal of the Korean Society of Radiology
• /
• v.13 no.5
• /
• pp.721-728
• /
• 2019

The purpose of this study was to evaluate the relationship between bone mineral density (BMD) and body composition (BC) or backmuscle strength (BS). Sixty-one participants were measured by BMD using ultrasound bone densitometry and Inbody for BC (i.e., SLM=Soft lean mass, SMM=Skeletal muscle mass, FS=Fitness score. et al.), BS after self-questionnaire for life cycle. This study was performed after approving Institutional Review Board and obtaining the informed concent from all participants. Participants was divided into two group by BMD T-score; $T-score{\geq}-1.0$, T-score<-1.0. Statistical analysis was performed by using SPSS ver. 22.0(USA), Spearma test for correlation between BMD and BC or BS. BMD or SLM, BS was increased with increasing physical activity or body mass index. SMM or SMM, FS of BMD $T-score{\geq}-1.0$ group was higher than that of T-score <-1.0 group as well as BS(p>0.05). BMD T-score was correlated positively with SLM(r= 0.424) or SMM(r= 0.431) in men, as well as in women(p<0.05). BS was correlated positively with SLM or SMM, FS. BS was significantly positive correlated with BMD in women group (r= 0.591, p= 0.001), but not in men group. We concluded that BMD is the relationship with SLM or SMM, as well as BS in women.

• Journal of Korean society of Dental Hygiene
• /
• v.24 no.2
• /
• pp.109-119
• /
• 2024

Objectives: This study aimed to confirm the correlation between oral frailty and health-related quality of life (HINT-8) among older adults in Korea. Methods: The data of 1,318 individuals aged ≥65 years who participated in the eighth Korean National Health and Nutrition Examination Survey (2019) were analyzed using complex sample statistical analysis. Results: Chewing discomfort was found to decrease the HINT-8 scores by 1.246, 1.324, and 1.089 times in the physical, social, and mental domains, respectively. Speech discomfort was found to decrease the HINT-8 scores by 1.275, 1.449, and 1.175 times in the physical, social, and mental domains, respectively. The HINT-8 scores of participants with ≤19 natural teeth were lower in the physical and social domains. Similarly, the HINT-8 scores of participants with brushing frequency of ≤2 were lower in the positive health domain. Non-use of oral hygiene products led to a reduction in the HINT-8 score in the social health domain. Conclusions: Oral frailty in older adults reduces the health-related quality of life. Thus, it is necessary to formulate policies to manage oral frailty in this population and develop specialized programs for the management of oral frailty.

• Journal of the Korea Academia-Industrial cooperation Society
• /
• v.19 no.11
• /
• pp.403-410
• /
• 2018

This study is to look at the risk of chronic diseases in adults and oral health behaviors affecting periodontitis by gender. This study selected 3,071 males aged 35 to 65, 4,273 females, and 7,344 people as the final subjects of the study using the sixth original data from the National Health and Nutrition Survey. In this study, subjective oral health classified under 'bad' presented 1.69 times(p<0.001) the risk of Periodontitis for males and 1.50 times(p<0.001) for females. There was a 2.01 times(p<0.001) of a risk of periodontitis for male and 1.40 times(p=0.001) of a risk for females. Smokers have a 1.68 times(p<0.001) of a risk for males and 2.07 times(p<0.001) of a risk for females, thus a higher risk for periodontitis for females. The risk of periodontitis was 1.44 times(p<0.001) of a risk for males and 1.30 times(p<0.05) for females when compared in normal hypertension. Obesity was at a rate of 1.199 times(p<0.05) as much for males in the non-military group, 1.202 times(p<0.05) that of females for periodontitis and putting females slightly more at risk. For diabetes, males were at risk of 1.28(p<0.05) whereas it being 1.53 times(p<0.05) for females, compared the average health female. In total, Males were found to be at the greatest risk of periodontitis, while women were at the highest risk for smoking. All parameters except smoking and diabetes show a higher risk of periodontitis for females. As males are more likely to have a higher risk of periodontitis than females, they are considered to be more interested in oral health care and need systematic oral health education and policies to prevent oral diseases.