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Effect of Density Separation after Pretreatment on Embryo Growth and Radicle Emergence of Carrot(Daucus carota L.) Seeds (당근 종자의 전 처리후 비중선이 배생장과 발아에 미치는 영향)

  • 민태기
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.37 no.2
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    • pp.134-140
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    • 1992
  • Carrot(Daucus carota L.) seeds (CV Danver 126) were primed and then separated by density differences to improve both the percentage and time of radicle emergence. Seeds for priming were soaked in aerated distilled water for 2 days (water imbibed), 25% solution of polyethylene glycol(PEG) 6000 for 6 or 10 days, salt solution of 0.2M KNO$_3$+0.1M $K_2$HPO$_4$ for 6 or 10 days, or mixed with Agro-Lig with 90% moisture content for 6 days and 70% moisture content for 6 or 10 days (SMP) at 2$0^{\circ}C$, respectively. The greatest embryo growth and the highest radicle emergence were observed from the seeds treated SMP with 90% moisture content for 6 days among the primed treatments. After the SMP treatment, the seeds were separated into density classes with a float-sink procedure using aqueous solution of Maltrin 600 with 0.02/cm$^3$ density increments. The lower density classes of the carrot seeds, the more embryo growth, the higher and the faster rates of radicle emergence were exhibited in order from 1.06 to 1.14 density classes of the carrot seeds treated SMP.

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Plant Regeneration from Immature Embryo and Bulb Scale Tissue of Hippeastrum hybridum (아마릴리스의 미숙배와 인편조직으로부터 식물체 재분화)

  • 최은경;박학봉
    • Korean Journal of Plant Tissue Culture
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    • v.25 no.1
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    • pp.27-31
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    • 1998
  • Immature hybrid embryos of H. hybridum, 'Picottee', 'White Christmas', 'Eldorado', 'Origin', 'Red Lion', 'elstar', 'Crypsy' were cultured on the MS medium supplemented with various concentrations of 2,4-D, NAA, BA and TDZ. Among the treatments, NAA were more effective for the shoot regeneration and bulblet formation than other treatment. Addition of 0.5 ㎎/L NAA was effective for bulblet induction from explant Shoot regeneration was most effective on the medium with 1.0㎎/L NAA and 2.0 ㎎/L TDZ. The addition of 1.0-2.0㎎/L TDZ induced numerous shoots per explant but strongly inhibited root development when compared to 1.0-2.0㎎/L BA. When bulb scale segments of 'Star Van Holland' was incubated, bulblet formation was the most effective on MS medium with 0.5㎎/L NAA.

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A Monitoring Survey on Pesticide Residues in Pears and Sweet Persimmons (배와 단감 중 농약 잔류에 관한 조사 연구)

  • 이해근;이영득;신용화
    • Journal of Food Hygiene and Safety
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    • v.3 no.3
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    • pp.131-137
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    • 1988
  • A pesticide monitoring survey was undertaken for the both 30 samples of pears and sweet persimmons from chief producing districts in1985 to evaluate pesticide residues in these agrcultural products and also to give basic informations in establishing maximum residue limits. Pesticide residues in pears were detected with fairly low in 7-60% of total samples. Even the maximum residue levels were only in the amount of 1/53-2/3 compared with maximum residue limits established in fruits in Environment Administration, Korea. Most of the residues (71-82%) was remained in peels of pears. Incase of sweet persimmons, captan, captafol, EPN, parathion, and phenthoate residues were also detected with fairly low in 3-20% of total samples. Presently, it is considered that pesticide residues in pears and sweet persimmons may be no harmful levels for food commodities.

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Changes in Growth and Bioactive Compounds of Lettuce According to CO2 Tablet Treatment in the Nutrient Solution of Hydroponic System (수경재배 양액 내 탄산정 처리에 의한 상추의 생육 및 생리활성물질 함량 변화)

  • Bok, Gwonjeong;Noh, Seungwon;Kim, Youngkuk;Nam, Changsu;Jin, Chaelin;Park, Jongseok
    • Journal of Bio-Environment Control
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    • v.30 no.1
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    • pp.85-93
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    • 2021
  • In hydroponic cultivation, in order to investigate the change of lettuce growth and physiologically active substances through CO2 tablet treatment in nutrient solution, we used a solid carbonated tablets commercially available in the Netherlands. The experiment consisted of 0.5-fold, 1-fold, and 2-fold treatment groups with no treatment as a control. As a result, the atmospheric CO2 concentration in the chamber after CO2 tablet treatment showed the highest value at 472.2 µL·L-1 in the 2-fold treatment zone immediately after treatment, and the pH in the nutrient solution decreased the most to pH 6.03 in the 2-fold treatment zone. After that, over time, the CO2 concentration and pH recovered to the level before treatment. Leaf width and leaf area of lettuce showed the highest values of 17.1cm and 1067.14 ㎠ when treated 2-fold with CO2 tablet, while fresh weight and dry weight of the above-ground part were highest at 63.87 g and 3.08 g in 0.5-fold treatment. The root length of lettuce was the longest (28.4 cm) in the control, but there was no significant difference in the fresh weight and the dry weight among the treatments. Apparently, it was observed that the root length of the lettuce was shortened by CO2 tablet treatment and a lot of side roots occurred. In addition, there was a growth disorder in which the roots turned black, but it was found that there was no negative effect on the growth of the above-ground part. As a result of analyzing the bioactive compounds of lettuce by CO2 tablet treatment, chlorogenic acid and quercetin were detected. As a result of quantitative analysis, chlorogenic acid increased by 249% compared to the control in 1-fold treatment, but quercetin decreased by 37%. As a result of comparing the DPPH radical scavenging ability showing antioxidant activity, the control and 0.5-fold treatment showed significantly higher values than the 1-fold and 2-fold treatments. This suggests that carbonated water treatment is effective in increasing the growth and bioactive compounds of hydroponic lettuce.

Effects of EGF, $\beta-ME$, Glucose, $O_2$ Concentrations and Fibroblasts Subculture on the Development of Porcine NT Embryos (EGF, $\beta-ME$, Glucose, $O_2$ 농도 및 Fibroblast Subculture가 핵이식 배의 체외발생에 미치는 영향에 관한 연구)

  • Quan J. H.;Wang A. G.;Kim S. K.
    • Journal of Embryo Transfer
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    • v.20 no.2
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    • pp.147-156
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    • 2005
  • 본 연구는 NCSU-23과 PZM-3 배양액에 EGF, $\beta-ME$와 glucose의 첨가가 돼지 난자의 체외성숙에 미치는 영향과 배양조건을 다르게 하여 계대배양한 섬유아세포를 이용한 핵이식배를 다른 배양액과 산소조건에서 배양하였을 때 체외발생율에 미치는 영향을 조사하였다. 핵이식 배를 20ng/ml EGF를 첨가 또는 첨가하지 않은 NCSU-23 및 PZM-3 배양액에서 배양하였을 때 배반포로의 체외 발생율은 각각 $12.0\pm1.3\%,\;9.6\pm1.9\%,\;10.9\pm2.1\%,\;9.1\pm2.3\%$였다. 핵이식 배를 $25{\mu}M\;\beta-ME$를 첨가 또는 첨가하지 않은 NCSU-23 및 PZM-3 배양액에서 144시간 배양하였을 때 배반포로의 체외 발생율은 각각 $9.6\pm1.7\%,\;7.3\pm2.3\%,\;11.9\pm1.8\%$$7.4\pm2.1\%$였다. $\beta-ME$를 첨가한 PZM-3 배양액에서 배양하였을 때 배반포로의 체외 발생율은 $\beta-ME$를 첨가하지 않은 배양액에서 배양한 배보다 높은 체외발생율을 나타냈다. (p<0.05). 핵이식 배를 1.5mM glucose를 첨가 또는 첨가하지 않은 NCSU-23 및 PZM-3 배양액에서 배양하였을 때 배반포로의 체외 발생율은 각각 $9.4\pm2.2\%,\;6.8\pm2.7\%,\;10.9\pm2.4\%$$8.9\pm2.6\%$였다. Glucose를 첨가한 NCSU-23과 PZM-3 배양액에서 배양하였을 때 배반포로의 체외 발생율은 glucose를 첨가하지 않은 배양액에서 배양한 배보다 높은 체외 발생율을 나타냈다. 핵이식 배를 NCSU-23 및 PZM-3 배양액과 $5\%$$20\%$ 산소 조건에서 배양하였을 때 배반포로의 체외 발생율은 각각 $11.1\pm1.8\%,\;9.8\pm1.4\%,\;12.5\pm1.6\%$$10.9\pml.5\%$였다. NUSU-23과 PZM-3 배양액에서 $5\%$ 산소 조건에서 배양하였을 때 $20\%$산소 조건에서 배양한 난자보다 높은 체외 발생율을 나타났다. 섬유아세포를 NCSU-23 배양액에서 배양하여 공여자세포로 이용하여 10 및 $11\~15\;passage$ 이내로 계대배양하였을 때의 융합율은 $60.0\~73.3\%,\;52.5\%$였다. 섬유아세포를 PZM-3 배양액에서 배양하여 공여자세포로 이용하여 10 및 $11\~15passage$ 이내의 계대배양시의 융합율은 $60.4\~75.1\%$$58.7\%$였다.

Convergence Study on the Employment Stress and Self-rated Oral Health Status in University Students (대학생의 취업스트레스와 자가구강건강상태에 관한 융합적 연구)

  • Kim, Min-A;Kim, Hyo-Jin;Lee, Yeon-Kyoung
    • Journal of the Korea Convergence Society
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    • v.11 no.3
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    • pp.127-133
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    • 2020
  • This study was to survey the relationship between employment stress and self-rated oral health status in university students from Sep. 2 to 27, 2019. Data were analyzed using the SPSS 22.0 program. In case of high employment stress, the comparative risk of self-rated oral health status was 1.7 times in Gingiva swelling, 1.46 times in ulcer and 1.53 times in hypersensitivity. After correcting the general characteristics (gender, age, grade, major), the comparative risk was 1.56 times for Gingiva swelling, 1.42 times for ulcer and 1.44 times for hypersensitivity. Therefore employment stress was found to be related to oral health and it is considered necessary to find ways to prevent and manage oral health by seeking various methods such as counseling support or stress control to reduce employment stress.

Inheritance and Cross Resistance of Bifenazate Resistance in Twospotted Spider Mite, Tetranychus urticae (Bifenazate 저항성 점박이응애의 유전과 교차저항성)

  • Yu Jeong-Soo;Seo Dong-Kyu;Kim Eun-Hee;Han Jong-Been;Ahn Ki-Su;Kim Gil-Hah
    • Korean journal of applied entomology
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    • v.44 no.2
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    • pp.151-156
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    • 2005
  • The development of resistance to bifenazate (resistance ratio of egg=40.3 folds) was found in population of the twospotted spider mite, Tetranychus urticae, collected from rose greenhouses in Chilgok, Gyeongbuk Province in December 2000. This population was selected for 4 years with bifenazate treatment (over 150 times), and showed 248.8 folds increase in resistance as compared to susceptible (S) strain. Inheritance of bifenazate resistant strain (R) and cross resistance of this strain to 9 acaricides were investigated. There were differences of susceptibility in the bifenazate concentration-mortality relationships in F1 progenies obtained from reciprocal cross with the S and R strain $(R{\times}S,\;S{\times}R)$. Degrees of dominance were 0.48 and 0.94 in adult females and eggs of $R{\times}S$ and -0.85 and -0.17 in adult females and eggs of $S{\times}R$, respectively. Inheritance type in the F1 progeny of $R{\times}S$ was incomplete dominant, and F1 progeny of $S{\times}R$ was incomplete recessive. These results suggest that inheritance of bifenazate resistance is controlled by a complete dominance. The R strain exhibited cross resistance to acequinocyl and fenpyroximate in adult females, and amitraz, emamectin benzoate, fenpyroximate, milbemectin, pyridaben and spirodiclofen in eggs. However they showed negatively correlated cross-resistance to emamectin benzoate and milbemectin in adult females, and abamectin in eggs.

Isolation of Lipoxyeenase Isozymes from Soybean Seeds (대두 Lipoxygenase 이성효소의 분리)

  • Song, Young-Sun
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.17 no.3
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    • pp.185-190
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    • 1988
  • Soybean lipoxyeenase isozymes were isolated from acetone-defatted soybean seeds(Glycine max [L.] Merr. variety AmSoy) by ammonium sulfate fractionation, eel filtration, and ion exchange chromatoeraphy. The final preparation of lipoxygenase-1 and -2 obtained was 19- and 32-fold purified, respectively, to the crude extract. But a considerable loss of total enzyme activity occurred during purification. On 7% polyacrylamide gel electrophosis at pH 9.0, employing lipoxigenase specific staining technique, lipoxyeenase-1, -2, and -3 showed distinctive Rf values of 0.38, 0.29, and 0.33, respectively.

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The Formaion of N-nitrosamine in Soy Sauce, Soybean Paste and Beer under Simulated Gastric Digestion (간장, 된장 및 맥주의 인공소화시 N-nitrosamine의 생성)

  • Kim, Kyung-Ran;Lee, Soo-Jung;Shin, Jung-Hye;Seo, Jong-Kwon;Shon, Mi-Yae;Sung, Nak-Ju
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.31 no.3
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    • pp.378-383
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    • 2002
  • The aim of the study was to analyze N-nitrosamine (NA) and its precursors in serveral fermented foods which were treated with nitrite, thiocyanate and ascorbic acid under simulated gastric digestion. Every analyzed sample contained nitrate, with levels ranging from 0.3 to 1.3 mg/kg, but nitrite was present at very low levels of less than 0.3 mg/kg. And other precursors of amines such as dimethylamine and trimethylamine were detected less than 0.5 mg/kg in every samples. N-nitrosodimethylamine (NDMA) was detected in the levels of <0.5 ∼ 2.7 ug/kg in soy sauce,1.5∼3.1 ug/kg in soybean paste and <0.5∼1.8 ug/kg in beer, while NDMA levels increased by 1.1∼4.5 times in the fermented foods which were digested under simulated gastric conditions.