• Proceedings of the Korean Society for Applied Microbiology Conference
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• 1976.10a
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• pp.190.4-190
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• 1976

Naringinase 생산 균주로 분리 선정된 Asp. niger S-1은 동시에 Hesperidinase도 강력히 생산함이 확인 되었으며 이 균의 효소학적 특성을 요약하여 1. 최적 반응 온도는 $60^{\circ}C이며$ $80^{\circ}C에서$ 30분 열처리 하여도 65%을 활성을 가지며 pH 5.0부위에서 최적반응과 안정성을 보였으며 Mg(이온)은 반응을 활성화 하였다. 2. Aceton을 60% 처리하여 조효소를 11배 정제하였으며 35%가 회수되었고 유안 0.4-0.6 포화로 48배 정제되었으며 13%가 회수되었다.

• Journal of Animal Science and Technology
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• v.50 no.1
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• pp.111-120
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• 2008

The average milk fat content in goat milk was 3.88% on yearly basis. The milk fat content of 3.8% during summer season was lower than 4.2% during winter season. Total solid content increased in proportion to milk fat. When goat milk was stored at 4℃ for 24 hr, short-chain FFA(C4:0~C10:0) and medium- and long-chain FFA(C12:0~C18:1) increased about 106% and 203%, respectively. Induced lipolysis of goat milk by homogenization increased short-chain FFA and medium- and long-chain FFA by 22% and 199%. When goat milk was treated with calf lipase, there was increase of short-chain FFA by 9 times greater than increase of medium- and long-chain FFA by 5.6 times. Treatment with lipases from Candida rugosa and Pseudomonas fluorescens resulted in increase of medium- and long-chain FFA by 34 and 162 times, respectively, which was greater than increase of short-chain FFA by 6 and 14 times, respectively. Lipolysis in goat milk stored at 4℃ for 24 hr was correlated with LPL activity in goat milk(r=0.5635). Off-flavor of goat milk was correlated with LPL activity(r=0.5777). Milk fat content was negatively correlated with LPL activity(r=－0.4627). Palmitic acid content in goat milk was correlated with off-flavor(r=0.7226).

• KSBB Journal
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• v.5 no.2
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• pp.183-189
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• 1990

An efficient method has been developed for the purification of urokinase from 1, 000 liter batches of human urine. The procedure involved precipitation of urokinase with 2mM zinc chloride, resuspension of the precipitate with 0.1M EDTA/0.5M Glycine solution, and CM-Toyopearl and benzamidine-Sepharose column chromatography. The purified urokinase was fully active and possessed a specific activity of 1.07$\times$105IU/mg. The recoveries ranged from 42 to 65% in several preparations(mean value was 51%). And the urokinase purified by this process consisted of about 13% of single chain urokinase (pro-urokinase) as evaluated by SDS-polyacrylamide gel electrophoresis in reducing condition and by S-2444 amldolytic activity under plasmin treatment.

• Journal of the Korean Society of Fisheries and Ocean Technology
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• v.25 no.2
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• pp.70-74
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• 1989

This paper, described on the maneuverabilities of M.S. Cheju 402, the stern trawler (159 G.T.), training ship of Cheju National University which carried out the turning circle tests at helm angle 35$^{\circ}$ and 10$^{\circ}$, 20$^{\circ}$, 30$^{\circ}$Ztests of her. The results obtained were as follows; 1. The advances of the starboard and port turning circles were 79.1m, about 2.6 times of the length of the ship, and 81.4m, about 2.7 times of it, respectively. 2. The rates of speed reduction were about 0.49 together, and mean values of turning angular velocity of her at helm angle 35$^{\circ}$ into the starboard and port sides were 4.3$^{\circ}$/sec and 4$^{\circ}$/sec during the turning movement. 3. Overshoot angles of starboard side werelarger than those of port side at all Z tests, and mean values of them of the starboard and port sides at 10$^{\circ}$, 20$^{\circ}$, 30$^{\circ}$Z tests were 110.5$^{\circ}$ 20.5$^{\circ}$, 28.5$^{\circ}$ respectively. 4. The maneuvering indices K and T of experimental ship at 10$^{\circ}$, 20$^{\circ}$, 30$^{\circ}$, Z tests were 0.755 and 3.468, 0.566, and 1.621, and 0.481 and 1.547 respectively. Consequently, the experimental ship showed that her turning ability was more in effective as the helm angle was becomed larger and her obeying ability was more effective as it was becomed larger.

• Spatial Information Research
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• v.18 no.2
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• pp.25-34
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• 2010

This study simulated flood inundation each frequency rainfall using GIS spatial information and FLUMEN model for part of Muju-Namdae Stream. To create geomorphology for the analysis of flood inundation, Triangle Irregular Network(TIN) was constructed using GIS spatial interpolation method based on digital topographic map and river profile data, unique data source to represent real topography of the river areas. And also flood inundation was operated according to the levee collapse to consider extremely flood damage scenarios. As the analysis of result, the inundation area in the left levee collapse showed more high as 3.13, 3.69, and 4.17 times comparing with one of right levee for 50, 100, and 200 year frequency rainfall and showed 1.00, 2.15, and 3.34 times comparing with one of right levee in the inundation depth with over 1.0 meter, which can cause casualties. As the analysis of inundation area of the inundation depth with over 1.0 meter, which can cause casualties in left levee collapse, it increased more high as 263% and 473% when 50 year frequency change into 100 and 200 year frequency. Also As the analysis of inundation area of the inundation depth with over 1.0 meter in right levee collapse, it increased high as 123% and 142% when 50 year frequency change into 100 and 200 year frequency. Especially, the inundation area of the inundation depth with 3.0~3.5m showed more high as 263% and 489% when 50 year frequency change into 100 and 200 year frequency. It is expected that flood inundation map of this paper could be important decision making data to establish land use planning and water treatment measures.

• Korean Journal of Organic Agriculture
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• v.28 no.2
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• pp.223-233
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• 2020

The aim of the present study was to investigate the suppressive effects of the bio-sulfur used by eco-friendly farms on the outbreak of citrus scab. To evaluate the inhibiting effect of bio-sulfur on citrus scab germ tube growth, the citrus scab pathogen Elsinoe fawcettiiwas cultured in PDB and agar media, and germ tube growth was observed after bio-sulfur treatment. At both 40 and 88 h after inoculation, germ tube formation was inhibited by 500-, 1000-, and 2000-fold diluted bio-sulfur, and at dilutions above 4000-fold, germ tube formation was observed, although growth was still inhibited, when compared to untreated cultures. Meanwhile, the occurrence of citrus scab on spring-flush leaves in the field was 40.3% in the untreated control and 5.3, 10.3, 12.3, 15.3, and 24.0% when treated with imibenconazole, 2-4 and 6-6 lime-Bordeaux mixtures, which are also used by eco-friendly farms, 500-fold diluted bio-sulfur, lime sulfur, and 1000-fold diluted bio-sulfur, respectively. The occurrence of citrus scab on citrus fruit was 79.3% in the untreated control and 4.0, 33.8, 42.0, 43.3, 44.8, and 78.0% when treated with imibenconazole, 2-4 lime-Bordeaux mixture, 6-6 lime-Bordeaux mixture, 500-fold diluted bio-sulfur, lime sulfur, and 1000-fold diluted bio-sulfur, respectively. Because citrus scab can infect citrus leaves as early as May, as the spring flush begins, preventative control should be implemented by mid- to late-April, thereby increase disease control and reducing both labor and farming costs.

• Journal of the Korean Society of Marine Environment & Safety
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• v.24 no.4
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• pp.381-388
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• 2018

This study proposes marine traffic volume estimation method considering ship speed, a factor excluded from the existing method. Ten days of GICOMS marine traffic data from Pyeongtaek and Dangjin ports was applied to this study. As a result, converted traffic volume with the proposed estimation method showed an increase of 4.41 (${\pm}0.99$) times or decrease of 0.59 (${\pm}0.04$) at most, compared with the existing estimation method. Average marine traffic congestion for each time applying the proposed estimation method showed an increase of 1.43 (${\pm}0.10$) compared with the existing estimation method. The maximum marine traffic congestion for each time was 1.62 (${\pm}0.34$) times higher compared with the existing estimation method. Marine traffic peak time, defined as the highest point of marine traffic congestion, was evaluated to be different from that of the existing method because of distribution of vessel speed. In conclusion, considering ship speed is necessary when estimating marine traffic volume to produce a practical estimate of marine traffic capacity.

• Korean Journal of Medicinal Crop Science
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• v.5 no.4
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• pp.289-293
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• 1997

The effects of plant regulators on direct somatic embryogenesis in liquid culture of Rehmannia glutinosa were investigated and the proper explant for direct somatic embryo formation was studied. Direct somatic embryos were induced from leaf segments culture in the MS liquid medium containing 0.5 mg/l of both IAA and NAA, while IBA of 1.0 mg/l was required for the same effect. Many somatic embryos were directly formed at the concentration of 2.0 mg/l cytokinin such as BA, kinetin and zeatin, but somatic embryogenesis was relatively poor at above or below this level. Relatively more somatic embryos were induced in the combination of 1.0mg/l IAA and 2.0mg/l zeatin. Formation of somatic embryos begun after 6 weeks on stem segments, while 7 weeks both on petiole and leaf. However, overall production of somatic embryos after 8 weeks was higher in leaf segment than that of stem segment.

• Korean Journal of Soil Science and Fertilizer
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• v.39 no.6
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• pp.357-365
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• 2006

The investigations were conducted to find out the situation of fertilizer use and the contents of soil chemical components on summer vegetable crops at 791 farmers' upland fields located in the parts of Gangwon-do, Gyengsangbuk-do and Jeollabuk-do of alpine area. Major vegetable crops were potato, Chinese cabbage, radish, carrot, onion, and cabbage. From the location surroundings cultivated alpine vegetable crops, the orders were Gangwon-do>Gyeongsangbuk-do>Jeollabuk-do part in the sizes of a fie1d area and the height above sea level, and Jeollabuk-do>Gyeongsangbuk-do>Gangwon-do part in the slope degrees. The soil texture was of wide distribution on sandy loam soil for Gangwon-do(76%) and Jeollabuk-do part(64%), and 1oam(42%) and sandy loam soil(35%) for Gyeongsangbuk-do part. From the numbers of investigated fields, the distribution of slope degree was wider than those of height above sea level in relation to location surroundings. The upland soils of 785 fields cultivated vegetable crops were sampled at 0~15 cm of top soil before seeding or transplanting and analyzed. On an average, pH, organic matter, available phosphate and exchangeable potassium, calcium, magnesium of soil were 5.7, $27.6g\;kg^{-1}$, $765mg\;kg^{-1}$, $1.16cmol_c\;kg^{-1}$, $6.1cmol_c\;kg^{-1}$, and $1.6cmol_c\;kg^{-1}$, respectively. The average cation exchange capacity(CEC) of 120 sites in Gangwon-do part was $9.2cmol_c\;kg^{-1}$. The content of organic matter, exchangeable potassium, exchangeable calcium and exchangeable magnesium was higher, while that of available phosphate was lower with slope degrees. And the content of major chemical components in carrot soil was lower in comparison with other crop soils. The average levels of N, $P_2O_5$, $K_2O$, livestock manure and lime fertilizer of 785 Belds applied by farmers were 335, 198, 244, 12,680 and $1,750kg\;ha^{-1}$, respectively, for summer vegetable crops in alpine area. The average amounts of $N-P_2O_5-K_2O$ fertilizers applied by farmers in 785 Gelds of vegetable crops were higher 1.7~2.0-4.2~7.0-1.4~2.0 times on potato, 1.4~1.6-4.6~8.3-3.5~4.2 times on Chinese cabbage, and 1.2~1.3-4.2~7.2-3.0~3.61 times on radish than the rates of NPK fertilizers based on soil testing for each crop.

• Korean Journal of Soil Science and Fertilizer
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• v.29 no.4
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• pp.385-388
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• 1996

Accumulation of soil chemical components in plastic-house is serious problem in Korea. This study was carried out to solve the problem by comparing the contents of soil chemical properties in plastic-house with that of upland field. The content of organic matter and available $P_2O_5($ in plastic-house soil were higher than that of upland field. Particularly, organic matter content in plastic-house soil (43g/kg) was 1.7times higher than that of upland soil (26g/kg), and content of available $P_2O_5($ (893mg/kg) in plastic-house: soil was 4.9 times higher than that of upland soil(183mg/kg). The content of exchangeable K, Ca and Mg in plastic-house soil were 0.5, 6.8 and 1.6cmol/kg and that of upland field soil were 0.5, 2.3 and 2.5cmol/kg, respectively.