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Monitoring conservation effects on a Chinese indigenous chicken breed using major histocompatibility complex B-G gene and DNA Barcodes

  • Tu, Yunjie;Shu, Jingting;Ji, Gaige;Zhang, Ming;Zou, Jianmin
    • Asian-Australasian Journal of Animal Sciences
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    • v.31 no.10
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    • pp.1558-1564
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    • 2018
  • Objective: We report monitoring conservation effect for a Chinese indigenous chicken (Langshan) breed using major histocompatibility complex (MHC) and DNA barcords. Methods: The full length of MHC B-G gene and mitochondrial cytochrome oxidase I (COI) gene in generations 0, 5, 10, 15, 16, and 17 was measured using re-sequencing and sequencing procedures, respectively. Results: There were 292 single nucleotide polymorphisms of MHC B-G gene identified in six generations. Heterozygosity (He) and polymorphic information content (PIC) of MHC B-G gene in generations 10, 15, 16, and 17 remained stable. He and PIC of MHC B-G gene were different in six generations, with G10, G15, G16, G17 >G5>G0 (p<0.05). For the COI gene, there were five haplotypes in generations 0, 5, 10, 15, 16, and 17. Where Hap2 and Hap4 were the shared haplotypes, 164 individuals shared Hap2 haplotypes, while Hap1 and Hap3 were the shared haplotypes in generations 0 and 5 and Hap5 was a shared haplotype in generations 10, 15, 16, and 17. The sequence of COI gene in 6 generations was tested by Tajima's and D value, and the results were not significant, which were consistent with neutral mutation. There were no differences in generations 10, 15, 16, and 17for measured phenotypic traits. In other generations, for annual egg production, with G5, G10, G15, G16, G17>G0 (p<0.05). For age at the first egg and age at sexual maturity, with G10, G15, G16, G17>G5>G0 (p<0.05). Conclusion: Combined with the results of COI gene DNA barcodes, MHC B-G gene, and phenotypic traits we can see that genetic diversity remained stable from generations 10 to 17 and the equimultiple random matching pedigrees conservation population conservation effect of Langshan chicken was effective as measured by these criteria.

Number Portability Introduction Way Between $3G\rightarrow2G$ ($2G{\rightarrow}3G$간 번호이동성 도입 방안)

  • Choi Seung-Gwon;Lee Byeong-Rok;Ji Hong-Il;Choi Woon-Soo;Jo Yong-Hwan
    • Proceedings of the Korea Contents Association Conference
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    • 2005.11a
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    • pp.210-218
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    • 2005
  • Recently, customers can select various service providers using number portability according to given select options such as quality of service and fare organization. Therefore it is possible that subscriber maintenance and quality competition are converted to fare competition which is real competition concept. However, it is time that we need number portability between $2G{\rightarrow}3G$ for activating 3G services because some important issues are closed such as domestic number portability, WiBro service provider selection. In this paper, we proposed applying method and necessity of $2G{\rightarrow}3G$ number portability Also, we analyzed $2G{\rightarrow}3G$ number portability examples of domestic and foreign countries and domestic market situation about abstract of 3G mobile communication service and number portability introduction between $2G{\rightarrow}3G$ for 3G service activation appearing newly in this research.

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Evaluation of Nutritional Characteristics of Platycodon grandiflorum Seeds (도라지 종자의 영양학적 특성 평가)

  • Kim, Yangji;Woo, Hyeryeon;Imm, Jee-Young;Kim, Seok Joong
    • Journal of the Korean Applied Science and Technology
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    • v.35 no.2
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    • pp.478-484
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    • 2018
  • In this study, proximate composition, crude fiber, reducing sugar, free sugars, organic acids, minerals and amino acids of Platycodon grandiflorum seeds were analyzed to evaluate its nutritional value. Moisture, crude protein, crude fat, crude ash and carbohydrate contents of seeds were 6.97, 26.05, 27.46, 3.78 and 35.74%, respectively. Crude fiber of 6.31% and reducing sugars of 1.54% were also determined. Sucrose(1,661 mg/100 g) and lactic acid(1,224 mg/100 g) were most abundant free sugar and organic acid, respectively. Both phosphorus and potassium were main minerals that contained more than 700 mg in 100 g seeds. Amino acids analysis of 100 g seeds showed that glutamic acid(3.45 g), arginine(2.51 g), aspartic acid(1.66 g), leucine(1.29 g), lysine(1.10 g), alanine(1.05 g) and glycine(1.04 g) were abundantly contained in order, while others were less than 1 g.

The Sorption Kinetic Studies and Development of Mixed Culture for Removal of Nonpoint Pollution Source (비점오염원 처리를 위한 혼합여재의 개발 및 흡착 Kinetic 연구)

  • Chung, Woojin;Lee, Sijin
    • Journal of the Korean GEO-environmental Society
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    • v.13 no.4
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    • pp.37-44
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    • 2012
  • This study investigated on the adsorption of nonpoint pollution source using the Sand, hydroxyapatite(HAP), Zeolite and mixed culture. The adsorption of nonpoint pollution source on Sand, hydroxyapatite(HAP), Zeolite and mixed culture was investigated during a series of batch adsorption experiments. After the batch absorption experiments analysed COD, T-N, T-P on adsorption water. The experimental data was analysed using the pseudo-first-order adsorption kinetic models. Langmuir and Freundlich isotherm models were tested for their applicability. The maximum adsorbed amount $(Q_{max})$ of COD were found to be sand 0.0511mg/g, HAP 0.1905mg/g, Zeolite 1.0366mg/g and Mixed media 0.7444mg/g. The maximum adsorbed amount $(Q_{max})$ of T-N were found to be sand 0.0159mg/g, HAP 0.0537mg/g, Zeolite 0.5496mg/g and Mixed media 0.1374mg/g. The maximum adsorbed amount $(Q_{max})$ of T-P were found to be sand 0.0202mg/g, HAP 0.1342mg/g, Zeolite 0.0462mg/g and Mixed media 0.1180mg/g. As a result, the mixed media was effective to remove nonpoint pollution source.

The Study of Zn, Cu, Mn, Ni Contents of Serum, Hair, Nail and Urine for Female College Students (일부 여대생의 혈청, 소변, 두발, 손톱 중의 아연, 구리, 망간, 니켈 함량에 관한 연구)

  • 승정자;윤영화
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.29 no.1
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    • pp.99-105
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    • 2000
  • The purpose of this study was to evaluat the Zn, Cu, Mn and Ni status and correlation of their concentrations in serum, urine, hair and fingernail in female college students. As the results are follows; The mean age of subjects was 22.5 years and height, weight and BMI were 160.1 cm, 51.0 kg and 20.0 respectively. The daily intake of energy 1769.5 kcal and the ratio of carbohydrate, fat and protein for energy is 60 : 20 : 15. The daily intake of energy (88.5%), vitamin B2 (86.1%), Ca (75.4%), Fe (58.3%) and Zn (63.0%) of subjects did not reach to Korean Recommended Dietary Allowance (RDA). The daily mean intake of Zn, Cu, Mn and Ni were 7.56 mg, 2.30 mg, 3.81 mg and 0.18 mg respectively. The concentrations of Zn in serum, urine, hair and nail were 85.6$\mu\textrm{g}$/dL, 391.2$\mu\textrm{g}$/day, 174.6$\mu\textrm{g}$/g and 102.4$\mu\textrm{g}$/g respectively and those Cu were 84.2$\mu\textrm{g}$/dL, 56.6 $\mu\textrm{g}$/day, 20.3$\mu\textrm{g}$/g and 4.3$\mu\textrm{g}$/g respectively. The concentrations of Mn in serum, urine, hari and nail were 0.2$\mu\textrm{g}$/dL, 1.1$\mu\textrm{g}$/day, 1.8$\mu\textrm{g}$/g and 1.6$\mu\textrm{g}$/g respectively and those of Ni were 0.6$\mu\textrm{g}$/dL, 24.5$\mu\textrm{g}$/day, 3.5$\mu\textrm{g}$/g and 3.1$\mu\textrm{g}$/g respectively. The daily intake of Mn showed the positive correlations with concentration of Mn in hair (p<0.05) and the daily intake of Ni was positively correlated with urinary exretion (p<0.05) and hair contents (p<0.001) of Ni. The more detailed studies about these trace mineral status should be required.

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Effect of Controlled Release Fertilizer on the Growth and Flowering of Oncidium 'Sweet Sugar' (완효성비료 시비가 온시디움 생육과 개화에 미치는 영향)

  • Kim, Si Dong;Lee, Hee Doo;Kim, Ju Hyoung;Kim, Tae Jung
    • FLOWER RESEARCH JOURNAL
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    • v.17 no.4
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    • pp.246-250
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    • 2009
  • This study was conducted to determine the effect of controlled-release fertilizer on growth of Oncidium. Leaf and pseudobulb length increased with controlled-release fertilizer from 2 g to 3 g treatment compared to hyponex and controlled-release fertilizer I g treatment, while leaf width and number of leaf was not significant among treatments. Plant weight increased with controlled-release fertilizer from 2 g to 3 g treatment compared to hyponex and controlled-release fertilizer I g treatment. Flowering date(bloomed October early) did not show significant difference among treatments. The number of flowers showed the most in controlled-release fertilizer 3 g treatment as 62.4 ea/plant, but was not different significantly among treatments. Flower stem length and width were also non-significant among treatments. Branching numbers increased in the controlled-release fertilizer 2 g and 3 g treatments. Mineral elements of shoot increased from 2 g to 3 g treatment rather than the controlled-release fertilizer 1g treatment. Especially, K content was higher compared to N and P content. Therefore, appropriate controlled-release fertilizer amount for Oncidium was recommended as 2 g.

Monolithic zirconia crowns: effect of thickness reduction on fatigue behavior and failure load

  • Prott, Lea Sophia;Spitznagel, Frank Akito;Bonfante, Estevam Augusto;Malassa, Meike Anne;Gierthmuehlen, Petra Christine
    • The Journal of Advanced Prosthodontics
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    • v.13 no.5
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    • pp.269-280
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    • 2021
  • PURPOSE. The objective of this study was to evaluate the effect of thickness reduction and fatigue on the failure load of monolithic zirconia crowns. MATERIALS AND METHODS. 140 CAD-CAM fabricated crowns (3Y-TZP, inCorisTZI, Dentsply-Sirona) with different ceramic thicknesses (2.0, 1.5, 1.0, 0.8, 0.5 mm, respectively, named G2, G1.5, G1, G0.8, and G0.5) were investigated. Dies of a mandibular first molar were made of composite resin. The zirconia crowns were luted with a resin composite cement (RelyX Unicem 2 Automix, 3M ESPE). Half of the specimens (n = 14 per group) were mouth-motion-fatigued (1.2 million cycles, 1.6 Hz, 200 N/ 5 - 55℃, groups named G2-F, G1.5-F, G1-F, G0.8-F, and G0.5-F). Single-load to failure was performed using a universal testing-machine. Fracture modes were analyzed. Data were statistically analyzed using a Weibull 2-parameter distribution (90% CI) to determine the characteristic strength and Weibull modulus differences among the groups. RESULTS. Three crowns (21%) of G0.8 and five crowns (36%) of G0.5 showed cracks after fatigue. Characteristic strength was the highest for G2, followed by G1.5. Intermediate values were observed for G1 and G1-F, followed by significantly lower values for G0.8, G0.8-F, and G0.5, and the lowest for G0.5-F. Weibull modulus was the lowest for G0.8, intermediate for G0.8-F and G0.5, and significantly higher for the remaining groups. Fatigue only affected G0.5-F. CONCLUSION. Reduced crown thickness lead to reduced characteristic strength, even under failure loads that exceed physiological chewing forces. Fatigue significantly reduced the failure load of 0.5 mm monolithic 3Y-TZP crowns.

Emission of Airbone Pollutants from Traditional Korean Fireplace (아궁이 사용에 의한 대기오염물질의 배출 특성)

  • Park, Sung Kyu;Lyu, Kun Jung;Choi, Sang Jin;Kim, Dae keun;Kim, Dong Young;Jang, Young Kee
    • Journal of Climate Change Research
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    • v.6 no.2
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    • pp.113-119
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    • 2015
  • Emission from the traditional Korean fireplace, or the under-floor heating and cooking device, can contribute significantly to airborne pollutants inventories. This study has systematically measured emissions of airborne pollutants from the fireplace when used different fuels such as firewood, agricultural crop residuals, household wastes. The results show that emission factors of airborne pollutants through the primary combustion of firewood were 3.22 g/kg for TSP, 2.93 g/kg for $PM_{10}$, 2.65 g/kg for $PM_{2.5}$, 174.19 g/kg for CO, 7.77 g/kg for NO, 0.15 g/kg for $SO_2$, 40.53 g/kg for TVOC and 0.03 g/kg for $NH_3$; from burning of agricultural crop residues, 2.85 g/kg for TSP, 1.38 g/kg for $PM_{10}$, 1.14 g/kg for $PM_{2.5}$, 126.47 g/kg for CO, 12.60 g/kg for NO, 0.20 g/kg for $SO_2$, 33.73 g/kg for TVOC and 0.02 g/kg for $NH_3$; and for household wastes, 10.52 g/kg for TSP, 8.52 g/kg for $PM_{10}$, 6.23 g/kg for $PM_{2.5}$, 72.86 g/kg for CO, 11.73 g/kg for NO, 0.20 g/kg for $SO_2$, 47.10 g/kg for TVOC and 0.20 g/kg for $NH_3$.

Comparison of the Food Quality of Hybrid Grouper Epinephelus fuyscoguttatus×E. labnceolatus and Hybrid Longtooth Grouper E. moara×E. lanceolatus (대왕범바리(Epinephelus fuscoguttatus×E. lanceolatus)와 대왕자바리(E. moara×E. lanceolatus)의 식품학적 품질비교)

  • Lee, Woo Jin;An, Byoung Kyu;In, Jung Jin;Han, Hyeong Gu;Park, Jong-Yeon;Bang, In-Chul;Shim, Kil Bo
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.55 no.2
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    • pp.129-136
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    • 2022
  • The nutrient composition of the muscle of the hybrid grouper (Epinephelus fuscoguttatus×E. lanceolatus, HG) and the hybrid longtooth grouper (E. moara×E. lanceolatus, HLG) was measured as a function of body weight in this study. The crude protein and lipid levels in HG were 21.0-21.2 g/100 g and 1.9-3.4 g/100 g, respectively. HLG contained 20.6-20.7 g/100 g protein, and 3.9-5.5 g/100 g crude lipid. The total amino acid contents of HG were 18,829.7-18,980.2 mg/100 g and that of HLG was 17,793.7-19,293.7 mg/100 g. The mean saturated fatty acid content was 0.61-1.09 g/100 g for HG and 1.27-1.77 g/100 g for HLG. Monounsaturated and polyunsaturated fatty acid levels were 0.59-1.02 g/100 g (Monoene), 0.67-1.11 g/100 g (Polyene) in HG, which were lower than the 1.21-1.78 (Monoene), 1.22-1.69 g/100 g (Polyene) found in HLG. The highest mineral content (K and P) of HG was 510.13-517.05 mg/100g and 231.59-247.67 mg/100 g, while that of HLG was 518.81-523.59 mg/100 g and 257.51-248.84 mg/100 g, respectively. In conclusion, there is potential for expanding the commercial utilization of HG and HLG as food resources as they are both nutrient-rich.

Study on the Elimination of Heavy Metal Pollutants in the Soil by Vascular Plant in the River Area (하천유역의 관속식물을 이용한 토양내의 중금속 오염원 제거에 관한 연구)

  • 강경홍;김인성
    • Journal of Korea Soil Environment Society
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    • v.1 no.2
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    • pp.23-34
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    • 1996
  • The biological magnification of Pb, Cu, Zn and Fe in vascular plant, Persicaria thunbergii, Trapa japonica and Nymphoides peltata natually grown at the river and riverside and relationship between the concentration of heavy metals in these vascular plants and in the soil were investigated in the Chonju river, Chonju city. The biological magnification values of these metals in P thunbergii were founded as follows ; Pb, Cu, Zn and Fe were 80.4~254.6$mu\textrm{g}$/g, 284.6~688.4$mu\textrm{g}$/g, 635.5~1979.4$mu\textrm{g}$/g and 1160.0~3590.9$mu\textrm{g}$/g respectively. In the case of T. japonica, Pb, Cu, Zn and Fe were 107.8~306.0$mu\textrm{g}$/g, 282.7~963.0p$mu\textrm{g}$/g, 1328.3~3546.$mu\textrm{g}$/g and 656.8~9944.0$mu\textrm{g}$/g and in N. peltata, 140.1~ 193.9$mu\textrm{g}$/g, 187.7~327.3$mu\textrm{g}$/g, 1126.6~1723.6$mu\textrm{g}$/g and 611.7~1914.6$mu\textrm{g}$/g respectively. The amount of the biological magnification of heavy metals in the leaf, stem and root of these plants was generally increased in the sequence of leaf

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