• Journal of Microbiology and Biotechnology
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• v.18 no.8
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• pp.1368-1376
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• 2008

In our previous study, the expression of active H-ferritins in Saccharomyces cerevisiae was found to reduce cell growth and reactive oxygen species (ROS) generation upon exposure to oxidative stress; such expression enhanced that of high-affinity iron transport genes (FET3 and FTR1). The results suggested that the recombinant cells expressing H-ferritins induced cytosolic iron depletion. The present study analyzes metabolic changes under these circumstances via proteomic methods. The YGH2 yeast strain expressing A-ferritin, the YGH2-KG (E62K and H65G) mutant strain, and the YGT control strain were used. Comparative proteomic analysis showed that the synthesis of 34 proteins was at least stimulated in YGH2, whereas the other 37 proteins were repressed. Among these, the 31 major protein spots were analyzed via nano-LC/MS/MS. The increased proteins included major heat-shock proteins and proteins related to endoplasmic reticulum-associated degradation (ERAD). On the other hand, the proteins involved with folate metabolism, purine and methionine biosynthesis, and translation were reduced. In addition, we analyzed the insoluble protein fractions and identified the fragments of Idh1p and Pgk1p, as well as several ribosomal assembly-related proteins. This suggests that intracellular iron depletion induces imperfect translation of proteins. Although the proteins identified above result from changes in iron metabolism (i.e., iron deficiency), definitive evidence for iron-related proteins remains insufficient. Nevertheless, this study is the first to present a molecular model for iron deficiency, and the results may provide valuable information on the regulatory network of iron metabolism.

• Journal of Satellite, Information and Communications
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• v.8 no.4
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• pp.135-141
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• 2013

Subway in Korea, most of a metropolitan public transport use only worth ten million people a day floating population of wireless communication can be considered HOT SOPT. These three operators' 2G/3G/4G / Wi-Fi / T-DMB frequency and the number of services to co-exist, and the subway train passengers and subway party for operation and management of the communication network is also the co-subway passenger safety and for this service for the effects of mutual interference of whether the verification was necessary. This research and testing different frequency / communication interference between services and testing to determine whether, through the subway authorities and mobile operators in each frequency-specific interaction check and there is no interference to the safety and quality of subway passengers communication services that can be verified as the data was found. The verification methods in the metro area, not just from one region to determine whether the interference with a common verification methods can be applied.

• Asian Pacific Journal of Cancer Prevention
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• v.17 no.2
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• pp.581-590
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• 2016

Resistance to anoikis, a cell-detachment induced apoptosis, is one of the malignant phenotypes which support tumor metastasis. Molecular mechanisms underlying the establishment of this phenotype require further investigation. This study aimed at exploring protein expression profiles associated with anoikis resistance of a metastatic breast cancer cell. Cell survival of suspension cultures of non-metastatic MCF-7 and metastatic MDA-MB-231 cells were compared with their adherent cultures. Trypan blue exclusion assays demonstrated a significantly higher percentage of viable cells in MDA-MB-231 than MCF-7 cell cultures, consistent with analysis of annexin V-7-AAD stained cells indicating that MDA-MB-231 possess anti-apoptotic ability 1.7 fold higher than MCF-7 cells. GeLC-MS/MS analysis of protein lysates of MDA-MB-231 and MCF-7 cells grown under both culture conditions identified 925 proteins which are differentially expressed, 54 of which were expressed only in suspended and adherent MDA-MB-231 but not in MCF-7 cells. These proteins have been implicated in various cellular processes, including DNA replication and repair, transcription, translation, protein modification, cytoskeleton, transport and cell signaling. Analysis based on the STITCH database predicted the interaction of phospholipases, PLC and PLD, and 14-3-3 beta/alpha, YWHAB, with the intrinsic and extrinsic apoptotic signaling network, suggesting putative roles in controlling anti-anoikis ability. MDA-MB-231 cells grown in the presence of inhibitors of phospholipase C, U73122, and phospholipase D, FIPI, demonstrated reduced ability to survive in suspension culture, indicating functional roles of PLC and PLD in the process of anti-anoikis. Our study identified intracellular mediators potentially associated with establishment of anoikis resistance of metastatic cells. These proteins require further clarification as prognostic and therapeutic targets for advanced breast cancer.

• Journal of Korean Society for Atmospheric Environment
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• v.33 no.6
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• pp.593-604
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• 2017

The suspended particulate matter(PM) was measured in subway tunnel of Seoul Line 1 to 9 in order to evaluate the pollution degree and characteristics of the PM in the subway tunnel. Also, to analyze the effect of outdoor aerosol concentration on the PM concentration of subway tunnels, the ambient PM concentration around the subway station was extracted by spatial analysis using $PM_{10}$ data of Seoul air pollution monitoring network. Finally, in order to understand pollution pattern in the Seoul subway tunnels, cluster analysis was performed based on input data set such as PM levels in tunnel, tunnel depth, length, curvature radius, outdoor ambient air pollution levels and so on. The average concentration of $PM_{10}$, $PM_{2.5}$, and $PM_1$ on subway tunnels were $98.0{\pm}37.4$, $78.4{\pm}28.7$, and $56.9{\pm}19.2{\mu}g/m^3$, respectively. As a result of the cluster analysis, tunnels from Seoul subway Line-1 to Line-9 were classified into five classes, and the concentrations and physical properties of the tunnels were compared. This study can provide a method to reduce PM concentration in tunnel for each pollution pattern and provide basic information about air quality control in Seoul subway tunnel.

• The KIPS Transactions:PartC
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• v.18C no.5
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• pp.361-368
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• 2011

In this study, we present a method for efficiently aggregating networks state information required to determine feasible paths in transport networks that uses the source routing algorithm for hierarchical QoS routing. It is proposed to transform the full mesh topology whose Service Boundary Line serves as its logical link into the star topology. This is an aggregation method that can be used when there are two or more QoS parameters for the link to be aggregated in an asymmetric network, and it improves the information accuracy of the star topology. For this purpose, the Service Boundary Line's 3 attributes, splitting, joining and integrating, are defined in this study, and they are used to present a topology transformation method. The proposed method is similar to space complexity and time complexity of other known techniques. But simulation results showed that aggregated information accuracy and query response accuracy is more highly than that of other known method.

• Convergence Security Journal
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• v.12 no.4
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• pp.3-8
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• 2012

Smartphones have all the recent technology integration and NFC (Near Field Communication) Technology is one of them and become an essential these days. Despite using smartphones with NFC technology widely, not many security vulnerabilities have been discovered. This paper attempts to identify characteristics and various services in NFC technology, and to present a wide range of security vulnerabilities, prevention, and policies especially in NFC Contactless technology. We describe a security vulnerability and an possible threat based on human vulnerability and traditional malware distribution technic using Peer-to-Peer network on NFC-Enabled smartphones. The vulnerability is as follows: An attacker creates a NFC tag for distributing his or her malicious code to unspecified individuals and apply to hidden spot near by NFC reader in public transport like subway system. The tag will direct smartphone users to a certain website and automatically downloads malicious codes into their smartphones. The infected devices actually help to spread malicious code using P2P mode and finally as traditional DDoS attack, a certain target will be attacked by them at scheduled time.

• IMMUNE NETWORK
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• v.2 no.4
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• pp.248-255
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• 2002

Background: TAP1 and TAP2 are two ABC transporter genes located within the class II region of the human MHC. Their protein products form a heterodimer whose function is to transport peptides from the cytoplasm into the endoplasmic reticulum. This study was performed to examine the polymorphism of TAP genes and the distribution of HLA-TAP haplotypes in the Korean population through family analysis. Methods: The subjects used in this study were 50 healthy Korean families consisting of 233 individuals. TAP1 (codons 333 and 637) and TAP2 (codons 379, 565, 577, 651, 665, and 687) typings were carried out by the PCR-restriction fragment length polymorphism (RFLP) method. HLA-DRB1 and DQB1 genotyping results from a previous study were used for HLA-TAP haplotype analysis. Results: The number (gene frequency) of TAP1 and TAP2 alleles detected were 3 for TAP1 (A 81.5%, B 17.0%, and C 1.5%) and 8 for TAP2 (A1 32.0%, A2 12.5%, B 34.0%, Bky2 6.5%, C 7.0%, D 3.0%, E 4.5%, and G 0.5%). Eleven TAP1-TAP2 haplotypes were observed with $frequency{\geq}1%$, among which 4 haplotypes (A-B, B-A1, A-Bky2, and C-E) showed weak but significant positive linkage disequilibrium (P<0.05). When DRB1-DQB1 haplotypes were extended to TAP1 and TAP2 loci, much diversification of haplotypes was observed: 19 different DRB1-DQB1 haplotypes formed 58 different haplotypes extended to TAP1 and TAP2 loci. These results add more evidence to the view that recombination hotspot is present within and around TAP gene region. Conclusion: The allele frequencies of TAP1 and TAP2 genes and the distribution of TAP1-TAP2 and HLA-TAP haplotypes were studied in Koreans based on a family study.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.29 no.4B
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• pp.411-423
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• 2004

This paper defines test methodologies and performance indices for SIP server system, and describes elements that can have influence on the test of SIP network equipments. Moreover, we implement a tester to evaluate the performance of SIP Servers such as Registrar and Proxy server. The performance indices for testing SIP servers are message processing rate, transaction delay, and call success probability. The parameters that can have an effect on the performance of SIP servers are user population, transport protocol, method of database access, method of DNS, call creation pattern, definition of transactions, and size of packets. We tested several SIP servers that act as Registrar, Proxy, and Redirect server using the implemented SIP tester, and, as a result, verified functions of the tester and performance indices and input parameters defined in this paper. Performance indices and methodologies presented in this paper can be used to evaluate SIP servers in NGN

• Journal of the Korean Institute of Electrical and Electronic Material Engineers
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• v.24 no.1
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• pp.81-88
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• 2011

A real time monitoring system from a PC has been developed which can be accessed through transmitted data, which incorporates an established low powered transport system equipped with a single chip combined with wireless sensor network technology from a three-axis acceleration sensor. In order to distinguish between static posture and dynamic posture, the extracted parameter from the rapidly transmitted data needs differentiation of movement and activity structures and status for an accurate measurement. When results interpret a static formation, statistics referring to each respective formation, known as the K-mean algorithm is utilized to carry out a determination of detailed positioning, and when results alter towards dynamic activity, fuzzy algorithm (fuzzy categorizer), which is the relationship between speed and ISVM, is used to categorize activity levels into 4 stages. Also, the ISVM is calculated with the instrumented acceleration speed on the running machine according to various speeds and its relationship with kinetic energy goes through correlation analysis. With the evaluation of the proposed system, the accuracy level stands at 100% at a static formation and also a 96.79% accuracy with kinetic energy and we can easily determine the energy consumption through the relationship between ISVM and kinetic energy.

• Smart Structures and Systems
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• v.26 no.1
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• pp.35-47
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• 2020

This paper proposes the use of transmissibility functions combined with a machine learning algorithm, Artificial Neural Networks (ANNs), to assess damage in a truss bridge. A new approach method, which makes use of the input parameters calculated from the transmissibility function, is proposed. The network not only can predict the existence of damage, but also can classify the damage types and identity the location of the damage. Sensors are installed in the truss joints in order to measure the bridge vibration responses under train and ambient excitations. A finite element (FE) model is constructed for the bridge and updated using FE software and experimental data. Both single damage and multiple damage cases are simulated in the bridge model with different scenarios. In each scenario, the vibration responses at the considered nodes are recorded and then used to calculate the transmissibility functions. The transmissibility damage indicators are calculated and stored as ANNs inputs. The outputs of the ANNs are the damage type, location and severity. Two machine learning algorithms are used; one for classifying the type and location of damage, whereas the other for finding the severity of damage. The measurements of the Nam O bridge, a truss railway bridge in Vietnam, is used to illustrate the method. The proposed method not only can distinguish the damage type, but also it can accurately identify damage level.