• Journal of Ginseng Research
• /
• v.44 no.4
• /
• pp.664-671
• /
• 2020

Background: Ginsenoside compound-Mc1 (Mc1) is a member of the deglycosylated ginsenosides obtained from ginseng extract. Although several ginsenosides have a cardioprotective effect, this has not been demonstrated in ginsenoside Mc1. Methods: We treated H9c2 cells with hydrogen peroxide (H₂O₂) and ginsenoside Mc1 to evaluate the antioxidant effects of Mc1. The levels of antioxidant molecules, catalase, and superoxide dismutase 2 (SOD2) were measured, and cell viability was determined using the Bcl2-associated X protein (Bax):B-cell lymphoma-extra large ratio, a cytotoxicity assay, and flow cytometry. We generated mice with high-fat diet (HFD)-induced obesity using ginsenoside Mc1 and assessed their heart tissues to evaluate the antioxidant effect and the fibrosis-reducing capability of ginsenoside Mc1. Results: Ginsenoside Mc1 significantly increased the level of phosphorylated AMP-activated protein kinase (AMPK) in the H9c2 cells. The expression levels of catalase and SOD2 increased significantly after treatment with ginsenoside Mc1, resulting in a decrease in the production of H₂O₂-mediated reactive oxygen species. Treatment with ginsenoside Mc1 also significantly reduced the H₂O₂-mediated elevation of the Bax:Bcl2 ratio and the number of DNA-damaged cells, which was significantly attenuated by treatment with an AMPK inhibitor. Consistent with the in vitro data, ginsenoside Mc1 upregulated the levels of catalase and SOD2 and decreased the Bax:B-cell lymphoma-extra large ratio and caspase-3 activity in the heart tissues of HFD-induced obese mice, resulting in reduced collagen deposition. Conclusion: Ginsenoside Mc1 decreases oxidative stress and increases cell viability in H9c2 cells and the heart tissue isolated from HFD-fed mice via an AMPK-dependent mechanism, suggesting its potential as a novel therapeutic agent for oxidative stress-related cardiac diseases.

• Journal of the Korean Association of Oral and Maxillofacial Surgeons
• /
• v.31 no.6
• /
• pp.461-467
• /
• 2005

Background. 5-(and-6)-carboxy-2',7'-dichlorofluorescein diacetate, succinimidyl ester mixed (CFSE) is the fluorescent labelling agent of living cells and used to trace the cells in vivo after transplatnation of various cells. The CFSE labelled cells can maintain fluorescence for up to 7 days after labelling. The MC3T3-E1 cell line (MC3T3) has been used for many studies about osteoblast, which is well known as a mouse preosteoblast. So the CFSE would be used to trace the transplanted MC3T3. However there are few reports about CFSE labelling of MC3T3. This study is aimed to know about adequate concenturation and incubation time of CFSE to MC3T3. Materials and methods. The MC3T3 was incubated in a humidified atmosphere of 95% air with 5% $CO_2$ at $37^{\circ}C$ using ${\alpha}$-minimal essential medium (${alpha}$-MEM) containing10% FBS and gentamycin. Ten mM CFSE solution in dimethylsulphoxide (DMSO: 1%) was diluted with phosphate buffered saline (PBS) and final concentration of culture medium was, respectively, 5, 10, 15, 20, 25 and 30 ${{\mu}M$. Then the MC3T3 was incubated with CFSE in a humidified atmosphere of 95% air with 5% $CO_2$ at $37^{\circ}C$ for 5, 10, 15, 20, 25, 30, 35, 40 and 45 minutes in each concentration. The fluorescence of CFSE labelled cells was analysed with a inverted fluorescence microscope. The duration of cell labelling was also studied. Trypan blue dye exclusion test was done for cell viability. Results. For concentration between 5 and 10 ${\mu}M$, CFSE did not significantly label the MC3T3 in vitro. The destruction of MC3T3 was observed at the concentration of 20 ${\mu}M$. In the concentration of 15 ${\mu}M$, the best labelling was obtained at an incubation period between 15 and 30 minutes. The MC3T3 labelled with an incubation period of 15 minutes at 15 ${\mu}M$ was still fluorescent 7 days after CFSE labelling. The mean cell viability was 95.93%. Conclusion. These results suggests an incubation period of 15 minutes at 15 ${\mu}M$ of CFSE provides best labelling of MC3T3 in vitro.

• Korean Journal of Environmental Agriculture
• /
• v.21 no.1
• /
• pp.57-68
• /
• 2002

In order to elucidate the effect of phenobarbital sodium (PB) and 3-methylcholanthrene (3-MC) on metabolism of insecticide carbofuran in rat. Carbofuran metabolites and its formation rates were determined when orally administered $^{14}C$-carbofuran alone and its combination with PB or 3-MC to rat. $^{14}C$-carbofuran administered orally, alone or in combination with PB or 3-MC, was secreted rapidly within 48 hrs. That is, 79.9 to 81.1% of the original radioactivity was secreted into the urine and 5.7 to 6.5% into the feces. The secretion rate was faster in the combined administration than that in carbofuran alone. Metabolites of carbofuran in main organs, urine, feces and blood of rat were largely 3-hydroxycarbofuran, 3-ketocarbofuran, 3-hydroxycarbofuran phenol, 3-ketocarbofuran phenol, and carbofuran phenol, the major ones being 3-hydroxycarbofuran and 3-ketocarbofuran, respectively, in all administrations of carbofuran alone, carbofuran+PB and carbofuran+3-MC. In addition, formation rate of the two major metabolites detected in the urine was 17.4% and 12.8%, respectively, when carbofuran alone was administered. Meanwhile, when carbofuran was administered with PB or 3-MC, they were 8.6% and 23.5, repectively. These results indicate that the oral administration of PB or 3-MC can reduce carbofuran toxicity by fastening and stimulating the carbofuran metabolism in rat.

• Journal of electromagnetic engineering and science
• /
• v.7 no.1
• /
• pp.1-6
• /
• 2007

Recently, Mc(Multi-code) modulation/demodulation(modem) technique has been explored for high speed data transmission in wireless environment. The conventional Mc modem generates some side effects such as allocating Walsh codes, which motivates to propose a novel Mc modem method with sub-code. Our proposed system should expanded the size of sub-code to provide high-rate data transmission, which also affect adversely to the performance of the system with high PAPR(Peak to Average Power Ratio). Thus, in this paper, we propose a novel pre-coded Multi-Dimensional HCOC(High Capacity Orthogonal Code) Mc modem technique to reduce the high PAPR, which enables the performance improvement. This proposed system can be easily designed by concatenating HCOC Mc modem with the generic Mc modem. The pre-coding technique that is used in this paper is CAC(Constant Amplitude Coding), that helps the system maintain the constant transmission power and reduce the maximum transmission power.

• Childhood Kidney Diseases
• /
• v.3 no.2
• /
• pp.237-240
• /
• 1999

The presence of polyostotic fibrous dysplasia of bone, hyperpigmented skin macules, and precocious sexual development in children is known as the McCune-Albright syndrome. In addition to the described in McCune-Albright syndrome, other endocrinopathies have been reported including hyperthyroidism, acromegaly, Cushing syndrome and vitamin D resistant rickets. The case describes a 6-year-old boy showing bony deformities due to polyostotic fibrous dysplasia, hyperpigmented skin macules, hyperthyroidism and vitamin D resistant rickets. The purpose of this report is to describe a patient of McCune-Albright syndrome with vitamin D resistant rickets which is very rare.

• Communications of the Korean Mathematical Society
• /
• v.11 no.2
• /
• pp.377-383
• /
• 1996

For a given separable space X which contains no copy of $C_0$ and a weakly compact T, we show that a Dunford integrable function $f : [a,b] \to X$ is intrinsically-separable valued if and only if f is McShane integrable. Also, for a given separable space X which contains no copy of $C_0$, a weakly compact T and a Dunford integrable function f we show that if there exists a sequence $(f_n)$ of McShane integrable functions from [a,b] to X such that for each $x^* \in X^*, x^*f_n \to x^*f$ a.e., then f is McShane integrable. Finally, let X contain no copy of $C_0$. If $f : [a,b] \to X$ is McShane integrable, then F is a countably additive on $\sum$.

• Annual Conference of KIPS
• /
• 2003.05b
• /
• pp.1497-1500
• /
• 2003

본 논문에서는 나카가미 페이딩 채널하에서 MC DS/CDMA 방식에 FH 방식을 적용한 FH MC/DS CDMA 시스템의 성능을 분석하였다. 나카가미 페이딩 지수 m과 MRC 다이버시티의 브랜치 수 L이 증가할 때 호핑 횟수 1과 4에 대한 분석결과 약 2.8dB의 성능차이를 보이고 있음을 앞 수 있었다. 호핑 횟수의 증가는 주파수 다이버시티 효과를 가져옴으로써 FH MC DS/CDMA 시스템에서 수신측의 부담을 줄이면서 BER 성능을 향상시킬 수 있음을 의미하며 따라서 호핑 횟수를 증가시킴으로써 부담 MC/DS CDMA 시스템의 성능을 개선할 수 있음을 입증하였다.

• The Journal of Korean Institute of Electromagnetic Engineering and Science
• /
• v.10 no.4
• /
• pp.487-497
• /
• 1999

In this paper, the performances of MC-CDMA systems for wireless ATM(WATM) is analyzed through computer simulations. The proposed MC-CDMA systems can support the 25 Mbps data rate with about 18.2 MHz bandwidth at 5 GHz carrier frequency, satisfying the specifications of wireless physical layer proposed by Wireless ATM Working Group(WATM WG). For the channel coding strategies, convolutional code, TCM and Turbo TCM(T-TCM) are explored and their performances are compared. From the simulation results, T-TCM becomes superior to the other coding schemes as interative decoding is performed.

• Journal of the Korea Institute of Information and Communication Engineering
• /
• v.7 no.3
• /
• pp.370-377
• /
• 2003

MC-CDMA is a digital modulation technique where a single data symbol is transmitted at multiple subcarriers which are orthogonal to each other. With this technique, frequency diversity can be achieved. The performance of MC-CDMA systems is analyzed, when data is transmitted over multi-path and Doppler fading channel. The performance of this technique, gauged by the average bit error rate, is numerically analyzed for the system with the application of antenna diversity and error control coding.

• Journal of the Korea Institute of Information and Communication Engineering
• /
• v.9 no.3
• /
• pp.504-509
• /
• 2005

This paper evaluates the BER performance of MC-DS-CDMA systems in frequency selective Rayleigh fading channel, considering the carrier frequency offset and the nonlinear high power amplifier. In the uplink MC-DS-CDMA, the BER performance is better than in the downlink. This paper presents the required output back-off of the nonlinear amplifier and value of frequency offset for good BER performance.