• Journal of Plant Biotechnology
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• v.5 no.4
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• pp.215-219
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• 2003

Optimal shoot regeneration and transformation conditions of root type chicory (Cichorium intybus L. var. sativus cv Cesare) were studied. Leaf explants were co-cultured with Agrobacterium tumefaciens, which contained NPTII as a selectable marker and a rice homeotic gene, OsMADS1, that encodes a MADS-domain-containing transcription factor. After one day of co-cultivation, explants were transferred to selection media consisting of MS basal medium supplemented with 0.5 mg/L BAP, 0.1 mg/L IAA, 70 mg/L kanamycin, and 250 mg/L cefotaxime. PCR and Southern blot analyses revealed stable integration of the OsMADS1 gene in the chicory genome. Four-teen original transgenic plants ($T_o$ plants) were acclimatized in the greenhouse and examined for their morphological characters. Most of the transgenic plants showed altered morphologies, such as short, bushy, and early-flowering phenotypes with reduced apical dominance. Additionally, half of the transgenic plants exhibited altered leaf shapes, and 4 out of 14 plants were sterile. These phenotypes were inherited by the next generation. Northern blot analysis confirmed expression of the OsMADS1 gene in both floral and vegetative organs.

• Korean journal of food and cookery science
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• v.30 no.2
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• pp.170-182
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• 2014

The study surveyed the dietary behavior, food intake, and food preference for breakfast of elementary, middle, or high school students, a total of 354 boys and 305 girls. The students skipped breakfast due to no appetite, followed by no time. Elementary school students ate grains and potatoes more often than middle or high school students(p<0.05), and bap was the most frequently consumed cereals, with juk and tteok as the least. High school students ate meat, ham, sausage and boiled fish cake (eomuk) more often than the elementary or middle school students(p<0.05). Middle school students ate milk and dairy products as well as vegetables and fruits more often(p<0.05) than other foods. Preference for fish and fresh vegetables and salad was significantly high in middle school students. The score of dietary behavior was the highest in elementary school students, followed by middle and high school students(p<0.001). As the students advanced to the upper grade schools, breakfast skipping increased, with lowered dietary behavior scores. The results suggest that a variety of breakfast menus and recipes should be developed at home and schools by considering the preference of students in order to reduce breakfast skipping and to improve their satisfaction with breakfast.

• Korean Journal of Plant Tissue Culture
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• v.22 no.3
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• pp.127-130
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• 1995

Japanese pepper (Zanthoxylum piperitum Dc.) tree of selected genotype was propagated by a two-step method. Among the media tested, BTM promoted shoot height growth and DKW revealed as superior to micro-canopy increment Multiple shoot formation was greatest when the single shoot were subcultured on medium containing 0.89 $\mu$M BA alone. The numbers of survival shoots could be markedly increased by acclimatization of the multiplied shoots itself in plastic Petridish (punctured with pin on the top) for two weeks and subsequently transplanting each shoot onto peat plug system. After transplanting the micropropagules onto pots, prickliness characteristics seem to be transmitted to all plane produced from selected genotype.

• Korean Journal of Plant Tissue Culture
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• v.27 no.6
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• pp.429-434
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• 2000

Isolated protoplasts from ginseng (Panax ginseng C. A. Meyer) callus tissue were cultured in modified MS media supplemented with various concentrations of dimethylsulfoxide (DMSO). The cell wall regeneration rate and cell division efficiency of the protoplasts were increased significantly by 1% DMSO treatment. However, there was no difference in the viability of protoplasts between the DMSO treatment and non-treatment. Transmission electron microscopy revealed that the microtubules were oriented in parallel manner to the plasmalemma after 3 days of culture in medium with 1% DMSO. Further, interconnected cellulose microfibrils were observed on the outer surface of the 3-day-cultured protoplasts by scanning electron microscopy These structures shown by electron microscopy were not observed in protoplasts cultured on DMSO-free media. This studies indicates that DMSO supplemented in culture media seemed to stimulate the cell wall regeneration and cell divisions of protoplasts by forming microtubule organizing centers (MTOC).

• Korean Journal of Plant Tissue Culture
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• v.22 no.3
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• pp.157-164
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• 1995

Intact mature zygotic embryos or their excised cotyledons of ginseng, were cultured on media containing various growth regulators such as auxin (2,4D, IAA) and cytokinin(BAP kinetin). In the culture of intact zygotic embryos, auxin inhibited germination but cytokinin did not Somatic embryogenesis occurred only from those of ungerminated embryos. In the culture of cotyledon segment, medium without growth regulators was the most appropriate to somatic embryogenesis. Somatic embryos were produced sporadically over the surfaces of zygotic embryos on medium containing auxin, while on medium without growth regulators, or media containing cytokinin, somatic embryos formed only on the proximal region of cotyledon. on medium containing 2,4-D, somatic embryos originated from multiple cells which comprised epidermal and subepidermal layers of cotyledon, which resulted in poly-somatic embryogenesis. When these somatic embryos were cultured on the same medium, the primary somatic embryos procured secondary embryos, which arose from epidermal or subepidermal single cells.

• Journal of Plant Biotechnology
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• v.47 no.3
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• pp.235-241
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• 2020

This is the first study to establish a complete protocol for micropropagation of Rehmannia glutinosa from root segments. The study involved investigating the effect of plant growth regulators on in vitro shoot regeneration and rooting and identifying substrates supporting survival and growth performance of ex vitro seedlings. A Murashige and Skoog (MS) medium containing 30 g/L sucrose for shoot induction and 0.2 mg/L indole-3-acetic acid (IAA), 1 mg/L 6-benzylaminopurine (BAP), and 1 g/L polyvinylpyrrolidone (PVP) for shoot multiplication resulted in the highest number of shoots per explant and shoot height. Applying a medium containing 0.5 mg/L IAA and 1 g/L PVP yielded optimal rooting of the shoots grown in vitro. Compost enriched with microbial inoculants and perlite enhanced seedling growth better than that with organic biofertilizer-free substrates (soil and sand). We recommend the continuous production of micropropagated R. glutinosa seedlings from root segments under the aforementioned conditions as a possible propagation technique for crops of this species.

• Biomedical Science Letters
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• v.9 no.1
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• pp.9-13
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• 2003

Currently bone biochemical markers are considered to be the best indicators of present and the future state of bone turnover. A recent study has reported that chlorella increases the bone mineral density (BMD) on postmenopausal women, but presently there are no studies on bone biochemical markers treated with chlorella dietary supplementation. The purpose of the present study was to assess the bone biochemical markers for the short term and long term treatment groups, and non-treatment group as a control. Twenty two postmenopausal woman were treated for four months and eighteen for one year with 4 gm of chlorella dietary supplementation per day, and then assessed bone biochemical markers from serum and urine samples. Bone turnover rates calculated with Osteocalcin (OC), bone specific alkaline phosphatase (BAP) as a bone formation markers and deoxypyridinoline (DP), cross-linked N-telopeptides of type I collagen (NTx) as a bone resorption markers, showed 1131$\pm$87% for control group, 61$\pm$11% for short term treated group and 190$\pm$101% for long term treated group. We conclude that chlorella dietary supplementation enhances the bone formation, and NTx as a single markers, OC/Dp as a single markers of bone turnover rate were very useful tools for determine the effectiveness of chlorella dietary supplementation (or the postmenopausal women.

• Journal of the Korean Institute of Landscape Architecture
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• v.35 no.4
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• pp.40-47
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• 2007

The objective of this study is to analyze the propriety of the Biotope Area Factor's(BAF) application and propose an improvement plan. The BAP system, initially started by Seoul metropolitan city, is being settled in Korea. The BAF originated from the Biotop $Fl{\ddot{a}}chen$ Faktor(BFF) system of Berlin Germany. It was established as part of the Landscape Plan for the ecological function recovery in the high density built-up area with a sense of environmentally friendly urban management. The study compared the BAF's present condition of Ganddong-Gu, Seoul with Seoul's BAF system. Some problems appeared from the system application. Firstly, it may cause ecological damage if the site ranges are more than current BAF system limits. Secondly, the application of the current BAF system has the possibility of general redevelopment, but the partial improvement considering current standards of the high density built-up area's paving section is impossible. Lastly, division of the application object and application type are not divided well. In addition, the Seoul BAF which is currently applied across the board is based on the destruction of the natural area and low density built-up area. Accordingly, to improve these problems requires a complementary system protecting the ecological function prior to the application of the BAF and with restricted application to high density of BAF system built-up area.

• Journal of the Korean Society for Marine Environment & Energy
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• v.4 no.3
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• pp.65-73
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• 2001

Herein, a theoretical method based on the catenary model Is applied to obtain the tension and drag forces acting on the trash curtain which is deployed at river for the prevention of floating debris inflow into the ocean. Under the assumption that fluid drag is perpendicular to the trash curtain, the tension and drag forces are uniform along the trash curtain. As a numerical model, the trash curtain is moored both symmetrically and asymmetrically with respect to the flow. The tension and drag forces on the trash curtain are investigated according to the change of Bap ratio and inclined angle of the trash curtain. Numerical results show that tension parameter is increased as the gap ratio is increased. It is found that tension parameter is reduced as the inclined angle is increased in the case of asymmetric deployment. The numerical model is applied to the specific problem for the trash curtain (200m) deployed at the Tancheon on the Han river. The maximum inflow velocity that anchor system can endure is 2m/sec.

• Journal of the Korean Society of Food Culture
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• v.29 no.1
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• pp.44-53
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• 2014

Various Korean healthy foods containing Deoduk were developed and standardized recipe. Sensory evaluations of Koreans and foreigners for these developed foods were performed by using the 5-point hedonic scale. The results of the sensory test on foods containing Deoduk were as follows: Deoduk bap (2.90~3.91), Deoduk seolgidduk (3.19~4.40), Deoduk gangjeong (3.00~4.60), riasted Deoduk with red pepper paste (2.73~4.20), Deoduk pine nut juice salad (2.40~3.96), and Seopsansam (3.91~3.81). Deoduk seolgidduk, developed by using a recipe for Sansambyeong which in a Chosun dynasty cook-book, was highly preferred by Africans, Japanese, and Koreans. In particular, all foreigners except Southeast Asians liked Deoduk gangjeong, and both Koreans and foreigners liked Seopsansam. Foreigners' experience of eating foods containing Deoduk was high at 'No' (68.9%), whereas Japanese (60.0%) had previous experience. Exactly 91.6% of Koreans responded 'Yes' to the question of whether or not Deoduk food is good for health, whereas the proportion of foreign respondents who said 'Yes' was 43.2%, 'No' was 5.4%, and 'I don't know' was 51.4%. Deoduk seolgidduk was preferred by Japanese, and most foreigners preferred Deoduk gangjeong and Seopsansam. Therefore, Deoduk could be developed as a healthy food for globalization of Korean food. However, as foreigners' awareness of Deoduk is low, we must positively promote the health function of traditional food ingredients and develop various menus suitable forforeigners' tastes for the globalization of Korean food.