• Proceedings of the Materials Research Society of Korea Conference
• /
• 2009.05a
• /
• pp.44.1-44.1
• /
• 2009

최근 경조직 재생 (hard tissue regeneration) 에 대한 연구가 활발히 진행되고 있다. 그러나 이와같은 연구는 결손도입의 어려움 및 이차적인 골절의 위험성 때문에 대형동물을 중심으로 진행되고 있으며, 그 결과 동물실험에 있어서 시간적 경제적으로 큰 리스크를 수반한다. 그러나 유전자 변형동물의 대부분은 마우스이며, 분자생물학적 관점에서 골재생의 과정을 이해하기 위해서는 마우스를 이용한 골재생 모델의 확립이 필요하다. 따라서 본 연구에서는 마우스를 통해 경조직 재생모델의 제작방법을 검토함과 동시에, 골재생부위에 대한 골질 (bone quality) 및 골양 (bone quantity) 평가의 방법을 수립하는 것을 목적으로 하였다. 골결손은 생후 8주의 마우스에 시술하였다. 치과용 드릴을 이용하여 경골 (tibia) 길이의 30 % 부근의 내측(medial) 면에서 골수강 (marrow cavity) 방향으로 $500\;{\mu}m\varphi$의 원주형 결손을 도입하였다. 시술 후의 골재생과정을 관찰하기 위해 ${\mu}CT$ (SMX-100CT: Simazu) 를 이용하여 주기적으로 촬영하였으며, 골양 (BV/TV) 의 회복과정은3D-bon (RATOC) 을 이용하여 정량적인 해석을 수행하였다. 그리고 재생부의 골질 (아파타이트 배행성; BAp orientation) 평가는 투과형micro-beam XRD (R-AXIS BQ: Rigaku)를 이용하여 수행하였다.

• Korean Journal of Weed Science
• /
• v.15 no.2
• /
• pp.160-165
• /
• 1995

Anthocyanin formation in callus cultures using Populus alba ${\times}$ Populus glandulosa was evaluated on basal MS medium supplemented with various levels of growth regulators, sucrose and nitrate concentrations. The highest yield of anthocyanin from cultured cells was produced under 5% sucrose, 1/8 strength of nitrate(12.5% of basic concentration) and combination of 1.0 mg/l IAA with 2 mg/l BAP, respectively. The high anthocyanin producing cell line no. 11 was selected among 15 cell lines, showing over 80% cells contained anthocyanin producing cells. From these cells, the highly productive red protoplast was isolated and the highest protoplast yield, $6.7{\times}10^6$ was obtained in enzyme combination IV which is composed of 2.0% cellulase, 0.5% macerozyme and 0.1% pectolyase.

• Mycobiology
• /
• v.32 no.3
• /
• pp.134-138
• /
• 2004

Water extracts of six plants, such as Allium sativum, A. cepa, Zingiber officinale, Platycodon grandiflorum, Oenanthe javanica, and Capsella brusapastoris, were tested in vitro for inhibitory activity against mycelial growth of anthracnose fungi, Colletotrichum gloeosporioides, C. dematium, and C. coccodes. Among the plant extracts, an Allium sativum extract has good inhibitory effects in all the fungi. Four phytohormones namely, IAA(indole-3-acetic acid), NAA(a-Naphthyl acetic acid), 2,4-D(2,4-Dichloro phenoxy acetic acid) and BAP(Benzyl adenine purine) were used to find out the role over mycelial growth of these fungi. All the concentrations of BAP have good inhibitory effect against mycelial growth of these fungi than that of other tested plant hormones.

• Journal of Software Assessment and Valuation
• /
• v.17 no.2
• /
• pp.39-46
• /
• 2021

To analyze and defend malware codes, reverse engineering is used as identify function location information. However, the stripped binary is not easy to find information such as function location because function symbol information is removed. To solve this problem, there are various binary analysis tools such as BAP and BitBlaze IDA Pro, but they are based on heuristics method, so they do not perform well in general. In this paper, we propose a technique to extract function information using LSTM-based models by applying algorithms of N-byte method that is extracted binaries corresponding to reverse assembling instruments in a recursive descent method. Through experiments, the proposed techniques were superior to the existing techniques in terms of time and accuracy.

• Anatomy and Cell Biology
• /
• v.56 no.1
• /
• pp.94-108
• /
• 2023

Cancer cell heterogeneity is a serious problem in the control of tumor progression because it can cause chemoresistance and metastasis. Heterogeneity can be generated by various mechanisms, including genetic evolution of cancer cells, cancer stem cells (CSCs), and niche heterogeneity. Because the genetic heterogeneity of CSCs has been poorly characterized, the genetic mutation status of CSCs was examined using Exome-Seq and RNA-Seq data of liver cancer. Here we show that different surface markers for liver cancer stem cells (LCSCs) showed a unique propensity for genetic mutations. Cluster of differentiation 133 (CD133)-positive cells showed frequent mutations in the IRF2, BAP1, and ERBB3 genes. However, leucine-rich repeat-containing G protein-coupled receptor 5-positive cells showed frequent mutations in the CTNNB1, RELN, and ROBO1 genes. In addition, some genetic mutations were frequently observed irrespective of the surface markers for LCSCs. BAP1 mutations was frequently observed in CD133-, CD24-, CD13-, CD90-, epithelial cell adhesion molecule-, or keratin 19-positive LCSCs. ASXL2, ERBB3, IRF2, TLX3, CPS1, and NFATC2 mutations were observed in more than three types of LCSCs, suggesting that common mechanisms for the development of these LCSCs. The present study provides genetic heterogeneity depending on the surface markers for LCSCs. The genetic heterogeneity of LCSCs should be considered in the development of LCSC-targeting therapeutics.

• Journal of the Korean Society of Food Science and Nutrition
• /
• v.42 no.12
• /
• pp.1958-1965
• /
• 2013

This study is conducted to investigate the contents of ${\beta}$-carotene and retinol in bap (e.g. gimbap, bokkeumbap, sushi, deopbap, bibimbap), guk (e.g. sundaeguk, seonjiguk), tang (e.g. galbitang, chueotang), and jjigae (e.g. dongtaejjigae, kimchi jjigae with port) by using saponification extraction and HPLC analysis. The samples were collected from six regions in Korea (Gangwon-do, Gyeonggi-do, Gyeongsang-do, Seoul, Jeonla-do, Chuncheong-do). In bap, the ${\beta}$-carotene contents of kimchi gimbap (234.459~719.180 ${\mu}g/100g$), bibimbap (200.091~489.867 ${\mu}g/100g$) and pork deopbap (228.876~778.591 ${\mu}g/100g$) were higher than that of sushi (0.000~41.234 ${\mu}g/100g$), and jajangbap (4.833~28.141 ${\mu}g/100g$). The retinol contents of bap was 0.000~60.418 ${\mu}g/100g$, among which, omelet rice (13.974~60.418 ${\mu}g/100g$) showed the highest amount of retinol. Among the analyzed guk (tang) and jjigae, higher contents of ${\beta}$-carotene were observed in chueoutang (346.261~843.947 ${\mu}g/100g$), kimchi jjigae with pork (178.558~352.604 ${\mu}g/100g$) and altang (169.443~175.287 ${\mu}g/100g$). The retinol of guk (tang) and jjigae were not detected to doganitang, gomtang, naejangtang, chueotang and soy sprout haejangguk.

• Journal of Life Science
• /
• v.16 no.3 s.76
• /
• pp.493-499
• /
• 2006

In this study, we investigated the effect of growth regulators, seed priming, and light condition for the germination and early growth in Snapdragon (Antirrhinum majus L.). The optimum concentration of growth regulator for the promotion of germinability turned out to be 250 uM of $GA_3$. The germination enhancement in combination of $GA_3+BAP$ was not significant. The optimum priming condition for the maximum germinability was 2 day treatment of 200 mM of $KNO_3$. which increased germination by 14% and shortened the day to germination by 3.5 days. The optimum temperature for germination was $15{\sim}25^{\circ}C$. The germination frequency was decreased to lower than 20% at $30^{\circ}C$, which showed that seeds of Snapdragon germinates better at low temperature than high temperature. The germination frequency was different at light condition; it was low at dark condition, but was increased by $15{\sim}20%$ at red light condition. The combination of $GA_3$ and seed priming showed better germinability than the single treatment of $GA_3$ and seed priming.

• Korean Journal of Medicinal Crop Science
• /
• v.2 no.3
• /
• pp.219-226
• /
• 1994

Immature ovule of intergeneric $F_1$ hybrid between Platycodon grandiflorum x Codonopsis lanceolata for producing embryogenic callus. somatic embryos and plant regeneration were cultured in vitro on various medium as well as MT(Murashige Tucker)medium treated with different concentration of plant growth regulators. Embryogenic callus induction was highest in the treatment of NAA 0.5 $mg/{\ell}$ and zeatin 0.01 $mg/{\ell}$ added on MT medium, whereas it was lower in treatments with auxins alone. MT medium were more effective in production of somatic embryos from incubated embryogenic callus. Most favorable plant growgh regulator for producing somatic embryos was 2. 4-D 0.5 $mg/{\ell}$ and zeation. BAP 0.01$mg/{\ell}$, but hormone-free and auxins alone were less effective. NAA 0.01$mg/{\ell}$ added with zeation 0.5 $mg/{\ell}$ was effective as high as NAA 0.01 $mg/{\ell}$ alone in normal plant regeneration from somatic embryo.

• Korean journal of food and cookery science
• /
• v.23 no.4 s.100
• /
• pp.511-529
• /
• 2007

This study was conducted to investigate traditional Kyunggi area Korean foods suitable for standardization and commercialization as well as to investigate the perceptions of experts about these foods, and to analyze the important value factor characteristics according to classified traditional food groups by residents of the Kyunggi(n=104)areas of Korea. We determined the degree of value of various traditional foods by asking respondents to 13 items based on a 5 point Likert scale, and used the total scores of the items to determine their value. Based on the results of the total scores and frequently chosen foods, we attempted to select the foods suitable for standardization and commercialization. The results are summarized as follows. For main dishes Ogok-bap was selected as the primary suitable for standardization and commercialization. Next were Incheon yeong yang bap, Hobak-beombek, Hobak-juk, Gesungpyeonsu, Tteokguk, Kimchibap, Kimchimandu, and bap, respectively. Of the side dishes Bossam-kimchi was selected as the primary suitable food for standardization and commercialization followed by Bindaetteok, Sondubu, Samgyetang, Baekkimchi, Doenjang-jjigae, Suwongalbi, Japchae, and Cheonggukjang-jjigae, respectively. Finally, for favorite foods, Duteop-tteok was selected as the primary food suitable for standardization and commercialization. Next were Jeungpyeon, Yakgwa, Yaksik, Maejakgwa, Ssuk-tteok, Yeongeunjeonggwa, Songpyeon, and Osaekdasik, respectively. This study shows that a resident's evaluation of a traditional Kyunggi Korean food is affected by the their sense of value of the food, as well as by the social structure, which includes the population, economic condition, food policies, industrial technology, and culture. This research was conducted to preserve traditional Kyunggi Korean foods and to improve this area's regional income from the prospect of sellable goods. We expect that this research to be used as basic data in the development of new commercial traditional foods by the food industry, by offering a better understanding of diverse consumer sense and to also be used with dietary education data relative to following traditional meal patterns for diverse age groups.

• Korean Journal of Plant Tissue Culture
• /
• v.24 no.2
• /
• pp.71-75
• /
• 1997

For the establishment of early selection of heat-tolerant clones through in vitro tuberization of true potato seeds, different temperature treatments for in vitro tuberization were investigated. The ratios of tuberization at 2$0^{\circ}C$ on var. Superior and DTO-33 treated with 5 mg/L of BAP and 500 mg/L of CCC, were 85% and 92%, respectively. At 3$0^{\circ}C$, the ratio of tuberization on DTO-33 was 37%, which revealed strongly heat-tolerant clone. In culture system of in vitro tuberization, the number of tubers per flask at 2$0^{\circ}C$ on non-subculture incubation was more than that on subculture incubation. The condition of non-subculture and short-day treatment for 4 weeks was good for production as 10.6 tubers per flask, which was very similar to that of long-day treatment. On the other hand, tuber diameter on long-day treatment was greater as 11.2 mm than on short-day treatment. Therefore, in vitro tuberization from true potato seeds could be induced under the condition of long-day treatment at darkness.