• Development and Reproduction
• /
• v.12 no.1
• /
• pp.1-8
• /
• 2008

Specific protocols to increase the differentiation of neuronal cells from embryonic stem (ES) cells have been well established, such as retinoic acid induction and lineage selection of neuronal cells. For the neuropathological studies, ES-derived neurons (ES neurons) must show normal physiological characteristics related to cell death and survival and should be maintained in vitro for a sufficient time to show insults-specific cell death without spontaneous death. When mouse ES cells were plated onto astrocytes monolayer after retinoic acid induction, most ES cells differentiated into neuronal cells, which were confirmed by the presence of specific neuronal markers, and the cultures were viable for at least four weeks. When these cultures were examined for vulnerability to glutamate excitotoxicity, ES neurons were vulnerable to excitotoxic insults mediated by agonist-specific receptors. The vulnerability to excitotoxic death increased with developmental age of ES neurons in vitro. Specific receptors for Neurotrophin and GDNF family ligands were present in ES neurons. GDNF and NT-3 could modulate the survival and excitotoxic vulnerability of ES neurons. The vulnerability and resistance to toxic insults, which are essential requirements of model culture systems for neuropathological studies, make ES neurons to a useful model culture system. Especially ES cell are highly amenable to genetic modification unlikely to primary neuronal cells, which will give us a chance to answer more complicated neurophysiological questions. Recently there was an outstanding attempt to explore the cellular toxicity using human ES cells (Schrattenholz & Klemm, 2007) and it suggested that ES cells could be a new model system for neurophysiological studies soon and go further a large-scale screening system for pharmacological compounds in the future.

• KOREAN JOURNAL OF CROP SCIENCE
• /
• v.65 no.2
• /
• pp.93-103
• /
• 2020

The average growth day of 11 maize varieties from planting to silking in the first cropping (FC) was 89.5 days and in the second cropping (SC) was 46.7 days, which was 43 days faster than in the FC. The average 100-kernel weight (100 KW) in the FC was 28.4 g and 18.3 g in the SC, which was approximately 36.4% lower than that in the FC. The average crude oil content of FC was 3.97% and SC was 3.08%, which was about 0.89% lower than that of FC. The composition of stearic and oleic acid was significantly higher in FC, whereas palmitic and linoleic acid were higher in SC; however, linolenic acid was not statistically different between the two crops. The crude oil content was negatively correlated with linoleic acid (FC -0.264^ns, SC -0.504**) and positively correlated with linolenic acid (0.526**). Unsaturated fatty acid (USFA) composition showed a significant difference between FC (83.48%) and SC (82.96%). Total phytosterol content was 598.3 mg/100 g and 701.9 mg/100 g in FC and SC, respectively, and showed significant difference by planting dates. The β-sitosterol content showed no statistical difference between the planting dates, but campesterol and stigmasterol were significantly higher in SC than in FC. Therefore, it was considered that the temperature condition during the ripening period affects the 100 KW of maize, and this leads to the variation in phytosterol content. However, among phytosterols, β-sitosterol was relatively little affected by the planting dates. USFA composition showed a significantly negative correlation with phytosterol content. Considering the results, the relatively high phytosterol content in SC was mainly because of the delay in progress of starch accumulation as daily air temperature decreased from the middle of the ripening period, whereas progress of phytosterol accumulation in the maize kernel was considered relatively faster than that of starch accumulation.

• Archives of design research
• /
• v.16 no.2
• /
• pp.179-188
• /
• 2003

We can discover some facts that vernacular design products with a property of volunteer culture coexist with ready-made products as an useful objects in the post-industrial society. Currently, few attempts had been made at studies of the vernacular design objects with understanding cultural context in post-industrial society, it is necessary to illuminate the relationships between the user with a role of design producer and the product viewed in culturally. In chapter 2, by examining documentary, t have been understood the definition, apprehensions, attributes linking creative use's culture with vernacular design culture, and distinctions between vernacular design and main stream design system. In chapter 3, by verifying products of everyday street and construction site, have been investigated the public characters and the differences compared with the past. In chapter 4, as a subtle example, have been analyzed design feathers and cultural characters of the products which children and infants culture generates by reflecting the socio-economic backgrounds and the culture from 60c and 70c to now according to the phases of the times. In conclusion, first, it seems quite probably that all of artificial objects exist a state of ready-made but that is not appropriate to correspond with user's whole behaviors. supplementally, it causes the phenomenon of vernacular design products. Secondly, it is reasonable to suppose that such main-stream design system and vernacular design go forward continually and crossly with coexistent relationships as high culture and sub culture. Third, three concepts: long-life design, ecology design and recycling revealed in vernacular design are useful distinguishing marks for the future design directions, moreover instant-response, fast and flexible process, three distinctions have a possibility of alternative process which can overcome the control-based process such as the systematic approach and the planning MTG in present. Finally, in children and infants culture, vernacular design products have changed the pure style products in the past into the modified techno playing products in the present. These should De a substitute product that supplyes variousness to ready-made design system and a design culture that maintains continually as a independent culture.

• Journal of Environmental Impact Assessment
• /
• v.25 no.6
• /
• pp.477-486
• /
• 2016

We investigated the growth pattern of Red-tongued viper snakes (Gloydius ussuriensis), which were captured from the islet of the Jeju Island, Gapado between April, 2006 and November, 2009. The results indicated that there were some snakes that grew relatively fast, but most snakes either almost did not grow or grew around 10mm in snout-vent length during one year period. High growth rates was April and June. Since the growth rate of snakes is highly correlated with their foods, these results implied that the feeding activity of Red-tongued viper snakes is high during this period compared to other months. In female, difference in body condition between good-conditioned and bad-conditioned snakes became large as time elapsed from April to June. The body condition of the male Red-tongued viper snakes improved with the progression of time from April till June. Many of the Red-tongued viper snakes were captured between April and June, while they were rarely captured between July and September. Some of the Red-tongued viper snakes were captured during the autumn season. This tendency was because snakes were rarely active during hibernation and peak summer seasons. Thus, Red-tongued viper snakes are active between April and June and between September and November. They then go into hibernation as the temperature dropped in November. Furthermore, the limitation of the movement period of the Red-tongued viper snakes restricted their feeding activities while foods became scarce, which ultimately restricted their overall growth rate. The growth rate of the snakes decreased with age. The snout-vent length of the Red-tongued viper snakes and growth rate showed a negative correlation (r = -0.591), however, it was not statistically significant due to small sample size. The findings from this study could provide meaningful information in the further study of the life cycle of Red-tongued viper snakes.

• 한국방재학회:학술대회논문집
• /
• 2011.02a
• /
• pp.28-28
• /
• 2011

국내의 건축물에 관한 통계정보는 국토해양부에 의해서 공표된 통계연보로서 2002년부터 매년마다 제공되고 있다.(국토해양통계누리, https : //stat.mltm.go.kr/potal/stat/yearReport.do) 건축물의 통계연보는 과거부터 2002년까지 매 3년마다 공표된 것으로 알려지고 있다. 이 통계연보에는 건축물 현황과 건축허가착공 통계가 수록되어 있다. 건축물 현황에는 2009년 말을 기준으로 기존 건축물이 전체 6,618,131동으로서 용도별, 층수와 연면적별 그리고 소유구분별 등의 구분에 따라 건축물의 동수에 관한 통계자료가 포함돼 있다. 건축허가 및 착공통계에는 용도별과 구조재료별의 분류에 따라 건축물의 동수에 관한 자료가 신축, 증축 개축 이전 대수선, 용도변경 등으로 분류되어 수록돼 있다. 한편 국내에서 건축물에 대한 내진설계기준이 1988년에 제정됨에 따라 법제화되었다. 이때 내진설계의 의무 대상건축물은 6층 이상 또는 연면적 1만$m^2$이상인 건축물로 규정되었다. 그 이후 내진설계 대상 건축물이 1996년부터 아파트는 5층 이상으로, 판매시설은 연면적 5천$m^2$이상으로 확대되었고, 2000년부터 숙박시설 오피스텔 및 기숙사는 5층 이상으로 확대되었으며, 2005년부터 내진설계 의무화 대상이 3층 이상 또는 1천$m^2$이상으로 확대되었고, 2009년부터 3층 이상 건축물의 구조안전의 확인을 위한 세부절차를 규정하여 내진설계의 실효성을 확보하고 있다. 이와 같이 내진설계의 대상 건축물이 내진설계기준을 제정한 이후 현재까지 시대의 흐름에 따라 6층 이상에서 5층 이상으로 다시 3층 이상으로 계속 확대되어왔다. 이런 환경에서 현재 시점에서 사용 중인 기존 건축물 중에 내진설계가 적용되지 아니한 건축물은 1988년 3월 1일 이전에 건축허가 된 건축물과 그 이후에 건축허가 된 3층 내지 5층 이하인 저층 건축물의 두 가지로 구분할 수 있다. 이들의 내진설계가 미적용 된 건축물에 대해서는 원칙적으로 내진보강 대책 수립 및 추진이 필요한 실정이다. 국내에서는 현재 지진재해대책법에 따라 지진재해로부터 국민의 생명과 재산을 보호하기 위하여 기존 시설물에 대한 내진대책을 추진하는 정책이 시행되고 있다. 앞으로는 이 정책의 일환으로 기존 건축물의 내진성능 확보를 위한 내진보강 대책이 구체적으로 추진될 전망이다. 이와 같이 기존 건축물에 대한 내진보강 대책을 수립하는 데는 무엇보다 그 대상 건축물의 수와 구조형식에 관한 정보가 필요하다. 이는 내진보강의 방법과 소요비용이 건축물의 층수 및 구조형식별 동수에 따라 크게 달라지기 때문이다. 이런 관점에서 살펴볼 때 내진대책의 수립에 필수인 기존 저층 건축물의 층수 및 구조형식별 동수에 관한 통계자료를 현재 건축물의 현황통계에서 손쉽게 찾아 볼 수 있으면 좋겠는데 현실은 그렇지 못한 실정이다. 현재 건축물의 현황통계에는 저층 건축물에 해당하는 층수에 관한 구분이 연대에 따라 다르고 구체적인 층수를 구분하기 어렵게 불분명한 항목으로 구성된 것과 구조형식별 분류항목이 없는 형편이다. 반면에 건축허가 및 착공통계자료에는 구조재료별 건축물 동수와 연면적에 관한 자료가 수록되어있고, 건축물 층수에 따라 분류된 통계자료는 없다. 이 연구에서는, 기존 건축물에 대한 내진보강 대책을 수립하는데 필요한 저층 건축물의 층수 및 구조재료별 동수 등에 관한 구체적인 정보를 파악하기 위하여, 건축물 통계연보에 수록된 건축물 현황통계자료에서 불명확하거나 결여된 정보를 건축허가 및 착공 통계자료로부터 얻은 정보로 보완과 보충하여 제시하고 있다. 따라서 이 연구는 기존 건축물의 내진보강 대책 수립에 필요한 5층 이하의 저층 건축물에 관한 층수별 및 구조형식별 동수에 관한 연도별 통계자료를 추정하여 제안하는데 그 목적이 있다.

• Journal of The Korean Society of Grassland and Forage Science
• /
• v.23 no.1
• /
• pp.7-12
• /
• 2003

This study was conducted to determine the influence of seeding date (May 3, 13, 23, June 2, 12) on the growth characteristics yield and feed value Azuki bean(Vigna angularis W.F. Wight) from May 3, 2002 to August 3, 2002 in Jeju province. Plant height was the longest (84cm) when seeded on 13 May and was the shortest (48.4cm) when seeded on 12 June. Numbers of branches and leaves per plant, stem diameter and weight of leaves and stems per plant were the same trend with plant height. Fresh forage, fry matter, crude protein and TDN(total digestible nutrient) yield per ha at were increased to 54.6MT, 7.7MT, 1.4MT and 4.5MT, respectively, whereas decreased as seeding date was delayed and then at seeding on 12 June were decreased to 11.5MT/ha 2.12MT/ha 0.4MT/ha and 1.3MT/ha, respectively The content of crude protein, crude fat, NFE(nitrogen free extract) and TDN were incresed 17.6%∼21.3%, 3.3%∼4.5%, 34.3%∼41.4% and 56%∼64.8%, respectively, as seeding was delayed from 3 May to 13 June. Whereas the content of crude fiber and crede ash were decreased 33.8%∼23.5% and 11%∼9.3％, respectively.

• Korean Journal of Food Science and Technology
• /
• v.50 no.5
• /
• pp.480-485
• /
• 2018

In this study, to identify single nucleotide polymorphisms (SNPs) associated with meat quality in Berkshire pigs, we performed RNA sequencing. A non-synonymous SNP (nsSNP) in the Complement component 9 (C9) gene was identified, and the association between meat quality traits and the C9 genotype was analyzed. The nsSNP in the C9 gene was located at c.942 G>T. In the dominant model, significant associations were observed between the SNP and meat quality traits such as CIE L, collagen content, moisture level, and $pH_{24h}$, whereas in the co-dominant model, significant associations were observed between the SNP and CIE L, collagen content, and protein content. In the recessive model, a significant association between the C9 genotype and the collagen content was observed. In addition, we identified the significant relationship between the C9 genotype and meat quality according to sex. These results indicate that the C9 SNP can be used as a genetic marker for improving pork quality.

• Korean Journal of Fisheries and Aquatic Sciences
• /
• v.33 no.2
• /
• pp.129-136
• /
• 2000

Differentiation and development of the digestive organ of the black sea bream, AcanHepagus schlegeli were studied by means of histological methods. The hatched lawn if TL(total length) $2.0 mm (n=10)$ had a yolk sac of $1,000{\times}590 {\mu}m$ and simple straight digestive tract, which was composed of cuboidal epithelium. In the pre-larval stage of TL $3.5 mm$, digestive tract could be distinguished into esophagus, stomach and intestine, and the exocrine glands were appeared in the pancreas. In this stage mucosal folds, eosinophilic granule cells and brush border were observed in the posterior intestine. Yolky materials were completely absorbed and the brush border was recognized in the free surface of anterior intestine in TL $3.7 mm$. In the stomach mucosal folds began to appear from TL $4.0 mm$. In this time the zymogen granules were recognized in the cytoplasm of pancreatic exocrine cells. In the post-larval stage ranged from $4.5 to 5.0 mm$ in TL, hepatic cords started to develop, and the mucous secretory cells of PAS positivewere observed at esophagus and intestine. In the post-larval stage ranged from $6.3 to 7.0 mm$ in TL, histological layer of esophagus and intestine could be distinguished into serous membrane, muscular layer, submucosal layer and mucosal layer. From over TL $9.0 mm$, stomach could be distinguished into cardiac, fundic and pyloric portion, and the gastric gland began to appear at mucosal fold of fundic stomach. In the juvenile stage ranged from $10.0 to 11.0 mm$ in TL, histological structures of esophagus and intestine were similar to those of adult. From over TL $15.0 mm$, histological structures of stomach were similar to those of adult. Structural and functional digestive organ of black sea bream was present from the juvenile stage ranged from $15.0 to 17.0 mm$ in TL.

• Journal of the Korea Academia-Industrial cooperation Society
• /
• v.19 no.1
• /
• pp.537-545
• /
• 2018

In this study, transcriptome analysis was conducted to collect various information from Fagopyrum esculentum and Fagopyrum tataricum during the early germination stage. Total RNA was extracted from the seeds and at 12, 24, and 36 hrs after germination of Jeju native Fagopyrum esculentum and Fagopyrum tataricum and sequenced using the Illumina Hiseq 2000 platform. Raw data analysis was conducted using the Dynamic Trim and Lengths ORT programs in the SolexaQA package, and assembly and annotation were performed. Based on RNA-seq raw data, we obtained 16.5 Gb and 16.2 Gb of transcriptome data corresponding to about 84.2% and 81.5% of raw data, respectively. De novo assembly and annotation revealed 43,494 representative transcripts corresponding to 47.5Mb. Among them, 23,165 sequences were shown to have similar sequences with annotation DB. Moreover, Gene Ontology (GO) analysis of buckwheat representative transcripts confirmed that the gene is involved in metabolic processes (49.49%) of biological processes, as well as cell function (46.12%) in metabolic process, and catalytic activity (80.43%) in molecular function In the case of gibberellin receptor GID1C, which is related to germination of seeds, the expression levels increased with time after germination in both F. esculentum and F. tataricum. The expression levels of gibberellin 20-oxidase 1 were increased within 12 hrs of gemination in F. esculentum but continuously until 36 hrs in F. tataricum. This buckwheat transcriptome profile analysis of the early germination stage will help to identify the mechanism causing functional and morphological differences between species.

• Journal of the Korean Society of Marine Environment & Safety
• /
• v.26 no.5
• /
• pp.523-530
• /
• 2020

Based on the results of harmful algal blooms (HABs) monitoring by the National Institute of Fisheries Science and local governments, the effects of changes in the marine environment on HABs are described. Since the beginning of HABs monitoring in 1972, they continued to increase from the 1980s to the 1990s. After the largest number of HAB incidents (109) in 1998; the trend declined until the 2010s. Most HABs in the 1970s were caused by diatoms. In the 1980s, coastal dinoflagellates caused HABs; Cochlodinium polykrikoides blooms have been occurring continuously since 1993. There are three HAB species that cause damage to fisheries in Korea. The high-density bloom of Karenia mikimotoi caused mass mortality in shellfish in Jinhae Bay in 1981. Karenia sp. blooms occurring around Tongyeong in 1992 killed aquaculture fish. Since the occurrence of the largest fisheries damage of KRW 76.6 billion in 1995 caused by C. polykrikoides blooms, they have been occurring continuously. The concentration of nutrients in coastal waters was the highest in the 1980s and has declined since the mid-1990s. This reduction in nutrient concentration is a good explanation for the decreasing number of HABs. Since 2016, a summer high water temperature of 30℃ or more has appeared, and the range and scale of C. polykrikoides blooms have been greatly reduced. In 2016, K. mikimotoi blooms occurred around Wando, Jangheung and Goheung and small scale blooms of C. polykrikoides occurred around Yeosu. There were no C. polykrikoides blooms in 2017; however, Alexandrium affine blooms occurred from Yeosu to Tongyeong. There was a small-scale blooms of C. polykrikoides in 2018 compared to those in the previous years. Our results show that reduction in nutrients and the high water temperature owing to climate change are a good explanation for variation in HABs in Korean coastal waters.