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Effect of 18 Irradiation on Neurotransmitters in the Brains of Goldfish Carassius auratus (18 방사성동위원소 피폭에 의한 금붕어(Carassius auratus)뇌의 신경전달물질 변화)

  • Park, Nam-Gyu;Go, Hye-Jin;Kim, Gun-Do;Lee, Jong-Kyu;Kil, Sang-Hyeong;Lee, Byung-Woo
    • Journal of Life Science
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    • v.22 no.8
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    • pp.1046-1051
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    • 2012
  • In order to investigate the changes in bioactive materials induced in goldfish brains by $^{18}F$ irradiation, the variations in the neurotransmitter levels in the whole brain were studied. The distance between the goldfish and 580 mCi of $^{18}F$ was about 4 cm, and the exposure lasted for 4 hrs. The absorption level calculated based on the distance, exposure time, and half-life of $^{18}F$ was approximately 2 Gy. After sacrifice by $^{18}F$ irradiation or untreated conditions, ten brains were dissected or immediately frozen, respectively. The tissues were extracted in acetic acid. After lyophilization, the samples were dissolved in distilled water and were further purified on a reverse-phase HPLC column. There were no differences in the intensities of the bioactive materials between $^{18}F$-exposed goldfish and control goldfish, while the only peak corresponded to 13 min, which indicated a significant increase in the irradiated brains. Our analysis has found that this compound is tryptophan. This result suggests that $^{18}F$ leads to changes in a classical neurotransmitter, tryptophan, in both the brains of control goldfish and goldfish contaminated by irradiation.

A Study on the prescriptions of 『Sanbeon-bang(刪繁方)』 (『산번방(刪繁方)』의 의방(醫方)에 관한 연구)

  • Kim, Do-Hoon;Jeong, Chang-Hyun
    • The Journal of Korean Medical History
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    • v.17 no.2
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    • pp.111-127
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    • 2004
  • This paper is mainly on the rsearch of the prescriptions of "Sanbeon-bang(刪 繁方)". For the research of prescription, investigated "Sanbeon-bang" from the side of symptoms of a disease, number of drugstuffs, table of contents, drugstuffs and acupuncture and moxibustion. With these investigation, made out a few tables, and with these tables made an attempt to understand the whole prescriptions of "Sanbeon-bang". "Sanbeon-bang" is of rich contents in internal medicine. Among internal medicine, related to Oro-Yukgeuk-Chilsang(五勞-六極-七傷) was most abundant. So we can guess "Sanbeon-bang" was a medical book specialized in exhaustion syndromes. It also deals with the Samcho-syndromes, as investigate from medical comments, has the perfect system of prescription in relative. From the investigation of the combination of drugstuffs, ascertained that, the number of simple prescription composed of one drugstuff was 38, combinational prescription composed of two drugstuffs was 9, minor prescription composed of three to five drugstuffs was 47, midum prescription composed of six to ten drugstuffs was 95, major prescription composed of eleven to twenty drugstuffs was 45, and mixed prescription composed over twenty drugstuffs was 1. Mentionable unique prescriptions in internal use were the forms of keeping in mouth. In external use they were fumigants, suppositories, powdered medicines, spraying the granular medication into the nasal cavity and eyedrops. And were abundant in soft extract(ointment) and plaster. In addition, there were the recordings of 18 types of cellulitis, types of incurable cellulitis and curing an illness by a charm. By the way, when comparing "Sanbeon-bang" with "Cheongeum-bang", all of which was quoted a lot in "Oedaebiyo-bang", "Cheongeum-bang" rather took medical comments of "Sanbeon-bang" than prescription. Although there were some prescriptons in "Sanbeon-bang" at the same category, "Cheongeum-bang" took another prescriptions which are more complexed than those of "Sanbeon-bang". In the same way, when comparing "Cheongeum-bang" with "Sonjinin-Cheongeum-bang(孫眞人千金方)", which didn't go through the correction of GyojeongUiseoguk of Bug-Song goverment, "Cheongeum-bang" often didn't take the prescriptions of "Sonjinin-Cheongeum-bang". Hence we can guess, "Cheongeumyo-bang" may have added a lot of prescriptions when undergoing the correction of of GyojeongUiseoguk. The total number of species of drugstuffs in "Sanbeon-bang" from the investigation was 284. The plant drugs were 208 species, the animal were 31 species, the minerals were 19 species and the other were 26 species. The prescriptions related to acupuncture and moxibustion in "Sanbeon-bang" were only moxibustional prescriptions. Which appeared one time per exhaustion and steaming of bone syndrome, Oro-Yukgeuk-Chilsang and Samcho syndrome. Appeared six times in muscle syndromes. But I cannot imagine the original form of acupuncture and moxibustion in "Sanbeon-bang" for deficiency of data.

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Differentiation of Vocal Cyst and Polyp by High-Piched Phonation Characteristics (성대낭종과 성대폴립 간의 고음발성 양상의 차이)

  • Lee, Jong-Ik;Jeong, Go-Eun;Kim, Seong-Tae;Kim, Sang-Yeon;Nam, Soon-Yuhl;Kim, Sang-Yoon;Roh, Jong-Lyel;Choi, Seung-Ho
    • Journal of the Korean Society of Laryngology, Phoniatrics and Logopedics
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    • v.23 no.1
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    • pp.48-51
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    • 2012
  • Background and Objectives : Vocal fold cyst is generally treated by surgical resection, it has a difference with vocal fold polyp, treated by conservative management first. Decrease in mucosal waves is known as main diagnostic criteria of vocal fold cyst. Sometimes there is a difficulty for diffrential diagnosis between cyst and polyp only by endoscopic examination. The purpose of the study is to identify the objective features of vocal cyst and polyp on the basis of voice analysis for the proper differential diagnosis, especially at high pitched phonation. Materials and Method : The voice analysis was done in 15 focal fold cyst patients and 42 vocal fold polyp. Parameters of perceptual assessment, acoustic and aerodynamic measure, and voice range profile were compared between two groups. Results : Vocal fold cyst patients showed significantly reduced MPT by acoustic and aerodynamic analysis, narrowed frequency-range and low maximun frequency by voice range profile analysis compared with vocal fold polyp patient. Maximun frequency 381 Hz is established for cut off value, differential diagnosis between cyst and polyp (ROC analysis, sensitivity 60%, specificity 68%). Conclusion : Voice analysis is helpful for differential diagnosis between vocal fold cyst and polyp, especially there is a difficulty for distinguish cyst from polyp at clinical situation by endoscopic examination. The result of decreased maximum frequncy at vocal fold cyst supports incomplete high-pitched phonation and falsetto regester at vocal fold cyst patients due to decreased mucosal wave, compared with vocal fold polyp patients.

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Accuracy Evaluation of Non-prism Total Station for Topographic Surveying (지형측량을 위한 무 프리즘 토털스테이션 정확도 평가)

  • Seo, Dong-Ju
    • Journal of the Korean Society of Surveying, Geodesy, Photogrammetry and Cartography
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    • v.24 no.5
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    • pp.433-441
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    • 2006
  • With a development of electrical technology in recent, it is possible to measure distance without direct contact to object using laser which launched at instrument and reflect from object. Furthermore, the advent of non prism total station brought the increment of application in many fields including not only road, airport, and harbors but also measurement and monitoring of structural displacement in construction fields. In this study, therefore, to evaluate accuracy of non prism total station, accuracy was analyzed by measuring certain distance which classified by both certain materials and angle of reflection. By this method, the derived values were applied to topographical survey for the efficient applicability. According to a study, result value of non prism total station was satisfBctory regardless of material when the angle of reflection was 90 degrees. RMSE increased when the angle of reflection are gradually increased to acute angle. In result of regression analysis using certain distance which classified by both materials and angles of reflection, there is relationship between distance and angle of reflection, but material has no relevance to the result value. When carrying out general topographical survey, proper application of non prism total station will go far conducting safe and prompt survey at the dangerous site such a road which have lots of traffic flow and rock joint which have high angles of inclination.

Effect of Clay Mineral Pegmatite and Vitamin A and Supplements on the Physico-Chemical Characteristics of Fattening Hanwoo Steers (거정석과 비타민 A 급여가 거세한우 등심육의 이화학적 특성에 미치는 영향)

  • Kim Byung-Ki;Go Si-Jang
    • Food Science of Animal Resources
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    • v.25 no.2
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    • pp.156-161
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    • 2005
  • This study was conducted to detennine the effect of dietary supplements of mineral powder (pegmatite) on the physico-chemical properties of M longissimuss for 24 Hanwoo steers. Each eight Hanwoo steers were randomly allotted to three treatments ; Control $(0\%)$, T1 (supplemented with pegmatite $2.0\%$ and vitamin A $0.2\%)$, T2 (supplemented with pegmatite $2.0\%$). Cooking loss was highest in control, while water holding capacity (WHC) was the highest in T2 (56.38) than the others. However, effect of the diets on meat color was not significantly different (p>0.05) Panel test scores of juiciness, tenderness and flavor of M longissinus were the highest for T1 $(4.6\~5.0)$ between the treatment group (p<0.05). The content of total fatty acid in M longissimus was the highest in T2 $(50.80\%)$ among the treatment group. Palmitic acid and oleic acid were siginificantly higher in T1 and control than those for the others(p<0.05). Unsaturated fatty acid (UFA) was highest in T2 $(58.57\%)$ than the others$(55.09\~55.64\%)$ (p<0.05).

Antioxidative Activities and Qualitative Characteristics of Substitute Tea using Salvia plebeia R. Br. (배암차즈기 차 제조와 항산화 활성 및 품질특성)

  • Jeong, Ji-Suk;Kim, Yong-Joo;Ahn, Eun-Kyung;Shin, Ju-Young;Go, Geun-Bae;Son, Byeong-Gil
    • Korean journal of food and cookery science
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    • v.31 no.1
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    • pp.41-52
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    • 2015
  • The substitute tea was manufactured from the leaves of Salvia plebeia R. Br. (SPR) that have anti-inflammatory and antioxidative activities. Total polyphenols and flavonoids in SPR were $71.8{\pm}4.74GAE\;mg/g$ and $38.7{\pm}2.95QUE\;mg/g$, respectively. DPPH radical scavenging activity of SPR water extract had an $RC_{50}$ of $16.9{\mu}g/mL$. SPR inhibited lipopolysaccharide (LPS)-induced nitric oxide (NO) production with an $IC_{50}$ of > $100{\mu}g/mL$. Medium and large leaves were used in the SPR substitute tea (SPRT). The water content was dissipated on roasting within approximately 10 minutes, and the tea obtained from fan-firing for a total 120 minutes was evaluated as most superior. Flavor was positively correlated with the taste and general preference. SPRT maintained its anti-oxidative activity without significant changes on roasting or fan-firing even after the heat treatment. The appropriate weight of a tea bag for one serving was 1.0 g. The study results indicated that SPR is a potential resource for the development of food or ingredients with anti-inflammatory properties.

Antioxidant Activity and DNA Damage Protective Effect of a Robinia pseudoacacia L. Flower Extract (아까시 꽃 추출물의 항산화 활성 및 DNA 손상 억제 효과)

  • Kim, Su-Jung;Seo, Go-Un;Seo, Bo-Young;Park, Eun-Ju;Lee, Seung-Cheol
    • Korean journal of food and cookery science
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    • v.27 no.4
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    • pp.99-106
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    • 2011
  • The antioxidant properties of Robinia pseudoacacia L. water and 70% ethanol extracts were evaluated by determining total phenolic content (TPC), DPPH radical scavenging activity (RSA), and reducing power (RP). The water extract showed higher TPC (9.07 mg/g gallic acid equivalents) and RP than those of ethanol extract, whereas ethanol extract had greater DPPH RSA. The R. pseudoacacia L. extracts also showed antigenotoxic effects for 200 ${\mu}M$ $H_2O_2$-induced DNA damage in human leukocytes. The 200 ${\mu}M$ $H_2O_2$-induced DNA damage decreased following treatment with the water extract. Reductions in DNA damage with 50 ${\mu}g/ml$ of the water and ethanol extracts were 46.5 and 32.4%, respectively.

A Study on Factors Affecting Formation of Social Capital for Volunteer Fire Brigade (의용소방대의 사회적 자본 영향요인 분석)

  • Woo, Seong-Cheon;Chae, Jin;Go, Gi-Bong
    • Fire Science and Engineering
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    • v.28 no.1
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    • pp.64-70
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    • 2014
  • A Volunteer Fire Brigade is a voluntary social services organization that performs various assistance works in fire fighting activities such as fire fighting and rescue of human lives on a part-time basis. Recently, it has also assumed an important role in the creation of security culture of local society. Besides, it provides diverse service activities related to security of local society. However, as this kind of volunteer fire brigade organization is not in a vigorous state, research for an improvement is being carried out in earnest. As part of a vitalization plan of the volunteer fire brigade, an attempt was made in this research to present an activation plan of the volunteer fire brigade through formation of social capital. Accordingly, efforts were made in this research to investigate and analyze an awareness of social capital among the volunteers and to present on this basis a plan for formation of social capital for the volunteer fire brigade of local society. As a result of the research, it turned out that social norm has the major effect on the formation of social capital for volunteer fire brigade. Therefore, it is our belief that an independent statute should be established to nurture and develop volunteer fire brigades. And when strong mutual trust among the volunteers are formed and efforts for devotion to local society are made on this basis, the security level of local society will be enhanced further.

A Numerical Study to Analyze Safety of Pressure Leakage Monitoring System of Gas Extinguishing Agent (가스소화약제 압력누기감시장치의 안전성 분석을 위한 수치적 연구)

  • Go, A-Ra;Lim, Dong-Oh;Son, Bong-Sei
    • Fire Science and Engineering
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    • v.30 no.4
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    • pp.103-110
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    • 2016
  • While the demand for the gas system fire extinguishers increases every year, there are insufficient safety measures for assessing the extinguishing performance, such as system safety and reliability in the preparation of increasing demand, which has emerged as a social problem. One of the most critical causes of accidents occurring with the gas extinguishing system is pressure leakage from the extinguishing agent storage container. This is considered to be one of the critical factors on which the success of fire suppression depends. In this study, its safety measure was studied, Because it was deemed urgently necessary. The newly developed pressure leakage monitoring system is a system monitoring storage condition, pressure, leakage and discharge of the storage container related to agent concentration, which is one of the critical factors for fire suppression. This was developed to be applicable to the $CO_2$ and HFC-23 systems. Therefore, for structural safety analysis, the safety performance was verified by the fluid structure coupling analysis of the safety problems that may occur when the pressure leakage monitoring system is applied to the gas fire extinguisher. For analysis programs, the FloEFD program from Mentor Graphics was used for computational fluid dynamics analysis and ABAQUS from Dassault Systems was used for structural analysis. From the result of numerical analysis, the structure of $CO_2$ did not develop plastic deformation and its safety was verified. However, plastic deformation and deviation issue occurred with the HFC-23 monitoring system and therefore verified the structural safety of pressure leakage monitoring system by data obtained from redesigning and adjusting the condition of numerical interpretation three times.

Surface Modification of Poly(vinylidene fluoride) Membranes using Surface Modifying Macromolecules (SMMs) and Their Application to Pervaporation Separation (SMMs을 이용한 고분자막의 표면개질과 이의 투과증발분리 연구)

  • Rhim, Ji-Won;Lee, Byung-Seong;Kim, Dae-Hoon;Lee, Bo-Sung;Yoon, Seok-Won;Im, Hyeon-Soo;Moon, Go-Young;Nam, Sang-Yong;Byun, Hong-Sik
    • Membrane Journal
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    • v.18 no.3
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    • pp.206-213
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    • 2008
  • Poly(vinylidene fluoride) (PVDF) membrane surfaces were modified using surface modifying macromolecules (SMMs). The Zonyl BA-L as SMM was used and the various PVDF membranes containing 0 to 2 wt% SMM were prepared. The resulting membranes were characterized through SEM, contact angle measurements and pervaporation separation of water-ethanol system. SMM layers were created in the surface regions of PVDF membranes by SEM images and the contact angles were increased more than untreated PVDF membranes. The pervaporation was carried out at 50, 60 and $70^{\circ}C$, and the PVDF membranes containing 1 and 2 wt% SMM were used for 10, 20, 50 wt% water in the binary water/ethanol mixtures and pure water. PVDF/2 wt% Zrlnyl BA-L membrane showed the permeability 5.3 $g/m^2hr$ and separation factor 287 at $50^{\circ}C$ for water : ethanol = 10 : 50 solution.