Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Effect of 18 Irradiation on Neurotransmitters in the Brains of Goldfish Carassius auratus

18 방사성동위원소 피폭에 의한 금붕어(Carassius auratus)뇌의 신경전달물질 변화

  • Park, Nam-Gyu (Department of Biotechnology, Pukyong National University) ;
  • Go, Hye-Jin (Department of Biotechnology, Pukyong National University) ;
  • Kim, Gun-Do (Department of Microbiology, Pukyong National University) ;
  • Lee, Jong-Kyu (Department of Physics, Pukyong National University) ;
  • Kil, Sang-Hyeong (Department of Nuclear Medicine, Pusan National University Yangsan Hospital) ;
  • Lee, Byung-Woo (Department of Materials System Engineering, Pukyong National University)
  • 박남규 (부경대학교 수산과학대학 생물공학과) ;
  • 고혜진 (부경대학교 수산과학대학 생물공학과) ;
  • 김군도 (부경대학교 자연과학대학 미생물학과) ;
  • 이종규 (부경대학교 자연과학대학 물리학과) ;
  • 길상형 (양산부산대학교병원 핵의학과) ;
  • 이병우 (부경대학교 공과대학 신소재시스템공학과)
  • Received : 2012.05.23
  • Accepted : 2012.07.06
  • Published : 2012.08.30
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Abstract

In order to investigate the changes in bioactive materials induced in goldfish brains by $^{18}F$ irradiation, the variations in the neurotransmitter levels in the whole brain were studied. The distance between the goldfish and 580 mCi of $^{18}F$ was about 4 cm, and the exposure lasted for 4 hrs. The absorption level calculated based on the distance, exposure time, and half-life of $^{18}F$ was approximately 2 Gy. After sacrifice by $^{18}F$ irradiation or untreated conditions, ten brains were dissected or immediately frozen, respectively. The tissues were extracted in acetic acid. After lyophilization, the samples were dissolved in distilled water and were further purified on a reverse-phase HPLC column. There were no differences in the intensities of the bioactive materials between $^{18}F$-exposed goldfish and control goldfish, while the only peak corresponded to 13 min, which indicated a significant increase in the irradiated brains. Our analysis has found that this compound is tryptophan. This result suggests that $^{18}F$ leads to changes in a classical neurotransmitter, tryptophan, in both the brains of control goldfish and goldfish contaminated by irradiation.

$^{18}F$ 방사성동위원소 피폭으로 금붕어 뇌에서 유도된 생리활성물질을 조사하기 위해 금붕어 뇌에 존재하는 신경전달물질의 생성 변화에 관한 연구를 수행하였다. $^{18}F$ 580 mCi를 납으로 밀폐된 공간에 두고 선원으로부터 4 cm 떨어진 위치에 금붕어들을 수조에 넣어 4시간 동안 노출시켰다. 거리, 노출시간 및 $^{18}F$의 반감기로부터 계산된 흡수선량은 약 2 Gy이었다. $^{18}F$에 의해 피폭된 금붕어 뇌와 피폭되지 않은 정상 금붕어의 뇌를 각각 10마리씩 절개하여 즉시 냉동 보관 하였다. 각각의 조직들은 초산으로 추출하였으며, 동결건조 후 샘플들은 증류수로 녹여 HPLC를 사용하여 물질을 정제하였다. $^{18}F$로 피폭된 금붕어와 피폭되지 않은 금붕어의 뇌에 존재하는 생리활성물질의 흡광도의 세기는 전체적으로 커다란 차이점은 없었지만, 방사선에 노출된 금붕어 뇌 추출물의 경우 13분대에 해당하는 물질 피크만이 정상 금붕어 뇌 추출물에 비해 매우 크게 증가하였다. 분석 결과, 이 물질은 트립토판(tryptophan, Trp)으로 밝혀졌다. 따라서 금붕어의 뇌에 존재하는 신경전달물질인 트립토판은 $^{18}F$ 방사성동위원소 피폭으로 금붕어 뇌에서 농도 변화가 유도된다는 것을 이 결과는 나타내고 있다.

Keywords

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