• Journal of Ginseng Research
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• 제13권2호
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• pp.254-259
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• 1989

A radioprotective ginseng protein fraction was obtained from Korean white ginseng powder by the following isolation and purification procedures: Tris-HCI buffer extraction, 70% ammonium sulfate fractionation, CM-rellulosr column chromatography, heat inactivation and Sephadex G-75 column chromatography. This fraction was further purified by Sepharose 4B and Sephadex G-150 column chromatographies. Three fractions obtained were subjected to Native-PAGE and SDS-PAGE using gradient gels and the silver staining method. Molecular weights of the native proteins and their subunits were estimated.

• 한국식품과학회지
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• 제36권2호
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• pp.361-364
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• 2004

은 이온이 불포화 지방산과의 착화합물을 만든다는 이론에 근거하여 은 이온 교환수지(SER)를 제조하였다. 제조한 SER을 비롯하여 silica gel, 질산은 함침 silica gel, 은 이온 제올라이트를 column 충전물로 사용하여 EPA와 DHA를 분리 농축하고 그 결과를 비교 분석하였다. SER과 silica gel의 9 : 1(w/w)혼합물을 충전물로 사용하였을 때 결과가 가장 좋았으며 이 경우 EPA와 DHA는 각각 27.9%와 49.1%로 농축되었고 수율은 각각 86.0%, 87.3%로 나타났다. SER만을 사용한 경우 EPA와 DHA는 각각 23.5%와 42.1%로 농축되었으며 이는 SER과 silica gel의 혼합 충전물 사용 시 보다 다소 낮은 결과이었다. 질산은 함침 silica gel의 경우 다른 충전물과 비교하여 농축율과 수율이 그다지 좋지 않았으나 EPA와 DHA의 분리 측면에서는 가장 우수한 결과를 나타내었다. SER은 재사용이 가능하고, 사용한 은 이온수지 자체도 쉽게 재생할 수 있을 뿐 아니라 사용한 은 이온도 AgCl 침전이나 $AgNO_3$로 회수가 용이하다는 점에서 다른 농축과정과 비교하여 훨씬 경제적이라 할 수 있다.

• 한국식품영양과학회지
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• 제26권6호
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• pp.1028-1032
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• 1997

Low-temperature crystallization method silver nitrate-impregnated silicic acid column chromatography were applied for the isolation of pure $\alpha$-linolenic acid(ALA) from perilla seed oil. ALA or 78% in purity(HALA; yield, 83%) was obtained from the fatty acid mixture(ALA, 65.7%) derived from perilla oil by the low-temperature crystallization method, when the mixture was frozen at -8$0^{\circ}C$ for 210min. ALA over 90% in purity(yield, 71%) was also obtained from HALA ethyl esters(ALA, 78%) by the silver nitrate-impregnated silicic acid column(100cm$\times$10cm, i.d.) chromatography. In addition, the silver nitrate-impregnated silicic acid could be semipermanently used for isolation of ALA, because $Ag^{+}$ ion was not dissociated from the stationary phase.

• Journal of the Korean Wood Science and Technology
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• 제21권3호
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• pp.22-29
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• 1993

나뭇잎으로부터 발생되는 저분자량 유기산을 이온크로마토그래피를 이용하여 분석하였다. 이 방법은 자연상태의 나뭇잎 (needle litter)을 유기용매의 추출 없이 그대로 이용할 수 있어서 사용하기에 편리하다. 본 논문에서는 이온 크로마토그래피의 사용방법과 침엽수잎(Pacific Silver Fir)의 부후정도에 따른 유기산의 종류와 농도의 변이를 간략하게 소개한다. 초기의 침엽수잎 여과액으로부터는 $C_2$에서 $C_4$까지의 아세트산(acetic acid), 프로피온산(propionic acid), 옥살산(oxalic acid) 그리고 포름산(formic acid) 등이 주종을 이루지만 부후정도가 커짐에 따라 방향핵을 가진 방향핵 유기산(aromatic organic acid)이 발생한다.

• Bulletin of the Korean Chemical Society
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• 제33권1호
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• pp.60-64
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• 2012

Silver(I) [bis(alkylthio)methylene]malonates were synthesized from the reaction of silver nitrate and potassium [bis(alkylthio)methylene]malonates. The structures of the Ag complexes were characterized with nuclear magnetic resonance (NMR), inductively coupled plasma atomic emission spectrometry (ICP-AES) and elemental analysis. Ag nanoparticles (NPs) were obtained from the decomposition of the Ag complexes in 1,2-dichlorobenzene at $110^{\circ}C$ without an additional surfactant. The average sizes of the Ag NPs are in the range of 5.1-6.3 nm and could be controlled by varying the length of the alkyl chain. The optical properties, crystalline structure and surface composition of Ag NPs were characterized with ultraviolet-visible (UV-visible) spectroscopy, transmission electron microscopy (TEM), X-ray diffraction (XRD), gas chromatography-mass spectrometry (GC-MS), X-ray Photoelectron Spectroscopy (XPS) and thermal gravimetric analysis (TGA).

• Environmental Analysis Health and Toxicology
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• 제12권3_4호
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• pp.15-22
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• 1997

This experiment was performed to examine the immuno-reactive characteristics of fish metal-binding protein, metallothionein (MT), and gain the practical understandings for the proposed use of fish MT as a biomarker. Liver MT induced by Cd in the silver carp was seperated and purified by gel filtration chromatography and ion exchange chromatography. The immuno-reactivity of fish MT was examined with 3 rabbit antisera. Fish MT showed little reactivity with rabbit anti-rat MT antiserum and a weak reactivity with anti-MT peptide antiserum while showed a strong reactivity with rabbit anti-fish MT antiserum. The time-course change of liver MT in the silver carp, after waterborne exposure to 1 ppm of Cd, was checked by Cd-hem method and established competitive ELISA. In both cases, the induction of liver MT showed a good increasing relationship with the exposure days. The results indicate that the fish MT can be developed as a useful biomonitoring means in the toxicological study and for the evaluation of water pollution.

• Environmental Analysis Health and Toxicology
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• 제11권1_2호
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• pp.11-17
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• 1996

The purpose of this experiment is to estabilish the sensitive and specific ELISA (enzyme linked immunosorbent assay) system for the detection of fish metallothionein (MT). Silver carp were injected with CA of 1-8mg/kg body wt. 4 times during 10 days. Silver carp was very tolerant species to CA. Cd induced MT in liver was seperated and purified by gel filtration chromatography and ion exchange chromatography and identified by spectrophotometry, native gel electrophoresis and western blot analysis. The rabbit antiserum was produced by immunizing rabbit with lyophilized MT, and the competitive ELISA system was estabilished for the detection of fish MT. In the present ELISA system, the detection limit was about 33 ng/ml. When this ELISA system was employed to determine the MT level in the supernatant sample of fish liver homogenate, the reaction curve showed a good parallel corelationship with the calibration curve over a certain dilution range. The results indicate that the competitive ELISA can be a useful tool for the detection of fish MT in the toxicological study and the evaluation of water pollution.

• 한국응용과학기술학회지
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• 제19권3호
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• pp.222-244
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• 2002

A mixture of methyl ester derivatives of fatty acids from the oils of pine nuts was well resolved to five fractions differing by degree of unsaturation by silver ion solid-phase extraction column chromatography ($Ag^{+}$-SEC). Polyunsaturated fatty acid with non-methylene interrupted conjugated double bond (NMiDB) radical held more strongly to silver ions in the column than methylene interrupted conjugated double bond (MiDB) one when they had the same number of double bonds. Although both the picolinyl ester and DMOX derivative provided clear mass ion species powerful enough to elucidate the structure of the polyunsaturated fatty acid (PUFA) with NMiDB and/or methylene interrupted conjugated double bond (MiDB) radical in the oils, the picolinyl ester of PUFA with NMiDB radical did not provide a cluster of mass ions neighboring diagnostic mass ions induced by the double bond in the proximal to the carboxyl group. However, the DMOX derivative of PUFA with NMiDB group as well as MiDB showed abundant mass ion species differing by gaps of 12 amu, which made it possible with greater ease to locate the double bonds in the molecule. The oil contained $C_{18:2{\omega}6}$ (46.2 %) and $C_{18:1{\omega}9}$ (25.4 %) as main components, and considerable amounts of PUFAs with NMiDB radical such as ${\Delta}^{5.\;9.\;12}-C_{18:3}$ (16.0 %), ${\Delta}^{5.\;9}-C_{18:2}$ (2.3 %) and ${\Delta}^{5.\;11.\;14}-C_{20:3}$ (0.8 %).

• 한국응용과학기술학회지
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• 제14권1호
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• pp.61-76
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• 1997

Triacylglycerols of the seeds of Ginkgo biloba have been resolved by high-performace liquid chromatography(HPLC} in the silver-ion and reverse-phase modes. The fatty acids were identified by a combination of capillary gas chromatography and gas-chromatography /mass spectrometry as the methyl and /or picolinyl ester. The main components are $C_{18:2{\omega}6}$(39.0mol%), $C_{18:1{\omega}7}$(asclepic acid 21.5mol%), and $C_{18:1{\omega}9}$(oleic acid, 13.8mol%). Considerable amounts of unusual acid such as $C_{20:3{\Delta}^{5,11,14}$ (5.7mol%), $C_{18:2{\Delta}^{5,9}$(2.8mol%), and $C_{18:3}{\Delta}^{5,9,12}$(1.6mol%), were checked. In addition, an anteiso-branched fatty acid, 14-methylhexadecanoic acid, was also present as a minor component(0.9 mol%). The triacylglycerols were separated into 17 fractions by reverse-phase HPLC, and the fractionation was achieved according to the partition numnber(PN) in which a ${\Delta}^5$-non methylene interrupted double bond($^5$-NMDB) showed different behaviour from a methylene interrupted double bond in a molecule with a given cahinlength. Silver-ion HPLC exhibited excellent resolution in which fractions(23 fractions) were resolved on the basis of the number and configuration of double bonds. In this instance, the strength of interaction of a ${\Delta}^5$-NMDB system with silver ions seemed to be weaker than a methylene interrupted double bond system. The principal triacylglycerol species are as follows ; $(C_{18:2{\omega}6)2}/C_{18:1{\omega}7}$, $C_{18:1{\omega}9}/C_{18:1{\omega}7}/C_{18:2{\omega}6}$, $(C_{18:1{\omega}7)2}/C_{18:2{\omega}6}$, $C_{16:1{\omega}7}/C_{18:1{\omega}9}/C_{20:3}{\Delta}^{5,11,14}$, $C_{16:1{\omega}7}/C_{18:1{\omega}7}/C_{20:3}{\Delta}^{5,11,14}$, $C_{18:1{\omega}9}/C_{18:1{\omega}7}/C_{18:2{\omega}6}$, $C_{18:1{\omega}9}/C_{18:2}{\Delta}^{5,5}/C_{20:3}{\Delta}^{5,11,14}$, $(C_{18:1{\omega}7)2}/C_{18:2{\omega}6}$ and $(C_{18:1{\omega}9)2}/C_{18:2{\omega}6}$, while simple triacylglycerols without $C_{18:2{\omega}6})_3$ were not present. Stereospecific analysis showed that fatty acids with ${\Delta}^5$-NMDB system and saturated chains were predominantly located at the site of sn-3 carbon of glycerol backbones. It is evident that there is asymmetry in the distribution of fatty acids in the TG molecules of Ginkgo nut oils.

• Bulletin of the Korean Chemical Society
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• 제17권2호
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• pp.143-146
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• 1996

A continuous potential pulse is applied to a silver-working electrode on a pulsed amperometric detector (PAD) for detection of free cyanide and sulfide. The moving phase is 0.1 M sodium hydroxide, 0.5 M sodium acetate and 5% (v/v) ethylenediamine mixture, and the flow rate is 0.7 mL/min. Optimized pulse conditions include a -200 mV (vs. Ag/AgCl reference electrode) detection potential(Ed) for 60 msec and 50 mV cleaning potential (Ec) for 120 msec. The silver working electrode surface is not poisoned by cyanide or sulfide, and the PAD maintains long-term stability without loss of sensitivity and reproducibility at these pulse conditions. The detection limit of cyanide and sulfide separated by ion chromatography using an anion exchange column is 0.1 ppm and 0.05 ppm, respectively.