Journal of Ginseng Research
- Volume 13 Issue 2
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- Pages.254-259
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- 1989
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- 1226-8453(pISSN)
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- 2093-4947(eISSN)
Further Purification of Radioprotective Ginseng Protein Fraction by Gel Filtration
Gel filtration에 의한 한방사선 인삼단백 분획의 정제
Abstract
A radioprotective ginseng protein fraction was obtained from Korean white ginseng powder by the following isolation and purification procedures: Tris-HCI buffer extraction, 70% ammonium sulfate fractionation, CM-rellulosr column chromatography, heat inactivation and Sephadex G-75 column chromatography. This fraction was further purified by Sepharose 4B and Sephadex G-150 column chromatographies. Three fractions obtained were subjected to Native-PAGE and SDS-PAGE using gradient gels and the silver staining method. Molecular weights of the native proteins and their subunits were estimated.
Keywords
- Radioprotective ginseng protein fraction;
- Sepharose 4B column chromatography;
- Sephadex G-150 column chromatography;
- native- PAGE;
- SDS- PAGE;
- gradient gel;
- silver staining method