• Journal of Microbiology and Biotechnology
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• v.16 no.11
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• pp.1753-1759
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• 2006

Paenibacillus sp. HY-8 isolated from the digestive tracts of the longicorn beetle, Moechotypa diphysis, produced an extracellular endoxylanase with a molecular weight of 20 kDa estimated by SDS-PAGE. The xylanase was purified to near electrophoretic homogeneity from the culture supernatant after ammonium sulfate precipitation, gel filtration, and ionexchange chromatography. The purified xylanase exhibited the highest activities at pH 6.0 and $50^{\circ}C$. The $K_m\;and\;V_{max}$ values were 7.2 mg/ml and 16.3 U/mg, respectively, for birchwood xylan as the substrate. Nucleotide sequence of the PCR-cloned gene was determined to have the open reading frame encoding a polypeptide of 212 amino acids. The N-terminal amino acid sequence and the nucleotide sequence analyses predicted that the precursor xylanase contained a signal peptide composed of 28 amino acids and a catalytically active 19.9-kDa peptide fragment. The deduced amino acid sequence shared extensive similarity with those of the glycoside hydrolase family 11 of xylanases from other bacteria. The predicted amino acid sequence contained two glutamate residues, previously identified as essential and conserved for active sites in other xylanases of the glycoside hydrolase family 11.

• Journal of Microbiology and Biotechnology
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• v.25 no.11
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• pp.1863-1870
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• 2015

Fibrinolytic enzyme genes (aprE2, aprE176, and aprE179) were introduced into the Bacillus subtilis 168 chromosome without any antibiotic resistance gene. An integration vector, pDG1662, was used to deliver the genes into the amyE site of B. subtilis 168. Integrants, SJ3-5nc, SJ176nc, and SJ179nc, were obtained after two successive homologous recombinations. The integration of each fibrinolytic gene into the middle of the amyE site was confirmed by phenotypes (Amy-, Spec^S) and colony PCR results for these strains. The fibrinolytic activities of the integrants were higher than that of B. subtilis 168 by at least 3.2-fold when grown in LB broth. Cheonggukjang was prepared by inoculating each of B. subtilis 168, SJ3-5nc, SJ176nc, and SJ179nc, and the fibrinolytic activity of cheonggukjang was 4.6 ± 0.7, 10.8 ± 0.9, 7.0 ± 0.6, and 8.0 ± 0.2 (U/g of cheonggukjang), respectively at 72 h. These results showed that construction of B. subtilis strains with enhanced fibrinolytic activities is possible by integration of a strong fibrinolytic gene via a marker-free manner.

• Journal of Microbiology and Biotechnology
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• v.28 no.5
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• pp.776-783
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• 2018

The agarase gene gaa16a was identified from a draft genome sequence of Gilvimarinus agarilyticus JEA5, an agar-utilizing marine bacterium. Recently, three agarase-producing bacteria, G. chinensis, G. polysaccharolyticus, and G. agarilyticus, in the genus Gilvimarinus were reported. However, there have been no reports of the molecular characteristics and biochemical properties of these agarases. In this study, we analyzed the molecular characteristics and biochemical properties of agarases in Gilvimarinus. Gaa16A comprised a 1,323-bp open reading frame encoding 441 amino acids. The predicted molecular mass and isoelectric point were 49 kDa and 4.9, respectively. The amino acid sequence of Gaa16A showed features typical of glycosyl hydrolase family 16 (GH16) ${\beta}$-agarases, including a GH16 domain, carbohydrate-binding region (RICIN domain), and signal peptide. Recombinant Gaa16A (excluding the signal peptide and carbohydrate-binding region, rGaa16A) was expressed as a fused protein with maltose-binding protein at its N-terminus in Escherichia coli. rGaa16A had maximum activity at $55^{\circ}C$ and pH 7.0 and 103 U/mg of specific activity in the presence of 2.5 mM $CaCl_2$. The enzyme hydrolyzed agarose to yield neoagarotetraose as the main product. This enzyme may be useful for industrial production of functional neoagaro-oligosaccharides.

• Korean Journal of Oriental Medicine
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• v.18 no.3
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• pp.119-126
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• 2012

1. Objectives Sasang constitutional medicine is unique in Korean traditional medicine. It diagnoses and treats patients based on his/her Sasang constitution (SC). Skin properties have been used as an effective diagnostic component in the classification of SC types in clinics. In this paper, we investigated the SC-based health relevance of skin elasticity and wrinkle properties. 2. Methods The skin elasticity and wrinkle of forearm and dorsal hand were measured in 299 elderly female subjects. To determine the subject's Sasang constitution, we adopted the classification results from a newly developed SC diagnostic tool. The health states of the subjects were scored by two Korean traditional medical doctors, by whom each subject was categorized either into the healthy state or the unhealthy state. 3. Results As a result, the elasticity hysteresis of forearm (E_HYS), the visco-elasticity (VE_MEAN), and the wrinkle frequency energy of backhand (W_HAND) showed significant differences between Taeum-in group and Soeum-in group. In case of the Soeum-in on unhealthy state, VE_MEAN was decreased significantly (p<.05). W_HAND and W_ARM_H of the healthy Taeum-in were less than those of the unhealthy Taem-in. 4. Conclusions In this study we showed that, for an elderly female population, skin elasticity and viscosity were significantly different not only between each SC type but also between healthy group and unhealthy group in each constitution. In particular, Soeum-in subjects were inferred to be superior in retaining skin softness when they were healthy, and Taeum-in subjects were easy to lose their firmness of skin surface when they became unhealthy.

• Journal of Trauma and Injury
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• v.33 no.2
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• pp.96-103
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• 2020

Purpose: In patients with trauma, rhabdomyolysis (RM) can lead to fatal complications resulting from muscle damage. Thus, RM must be immediately diagnosed and treated to prevent complications. Creatine kinase (CK) is the most sensitive marker for diagnosing RM. However, relying on CK tests may result in delayed treatment, as it takes approximately 1 hour to obtain CK blood test results. Hence, this study investigated whether the neutrophil-to-lymphocyte ratio (NLR) could predict RM at an earlier time point in patients with trauma, since NLR results can be obtained within 10 minutes. Methods: This retrospective study included 130 patients with severe trauma who were admitted to the emergency room of a tertiary institution between January 2017 and April 2020. RM was defined as a CK level ≥1,000 U/L at the time of arrival. Patients with severe trauma were categorized into non-RM and RM groups, and their characteristics and blood test results were analyzed. Statistical analysis was performed using SPSS version 26.0 for Windows. Results: Of the 130 patients with severe trauma, 50 presented with RM. In the multivariate analysis, the NLR (odds ratio [OR], 1.252; 95% confidence interval [CI], 1.130-1.386), pH level (OR, 0.006; 95% CI, 0.000-0.198), presence of acute kidney injury (OR, 3.009; 95% CI, 1.140-7.941), and extremity Abbreviated Injury Scale score (OR, 1.819; 95% CI, 1.111-2.980) significantly differed between the non-RM and RM groups. A receiver operating characteristic analysis revealed that a cut-off NLR value of 3.64 was the best for predicting RM. Conclusions: In patients with trauma, the NLR at the time of arrival at the hospital is a useful biochemical marker for predicting RM.

• Journal of Environmental Health Sciences
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• v.11 no.2
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• pp.17-27
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• 1985

The objectives of this study is to examine efficiency of swinery wastewater treatment by trickling filters' pilot plant. The results of this study are as follows: 1. The characteristics of sample. The BOD$_5$ was from 2,450 to 2,880mg/l, COD(KMnO$_4$ acid method) was from 910 to 1,064mg/l, and SS was from 920 to 990mg/l. The pH of influent was from 7.3 to 7.6, and the temperature of water was from 17.0$\circ$C to 22.5$\circ$C. 2. For experiment by recirculation, the BODs removal efficiency is 65.2% at recirculation ratio (r)=0, and 70.7% at r=1. The ramoval efficiency of this study is higher than NRC formula of U.S.A.. The recirculation is not significant effect on removal efficiency. 3. For experiment by hydraulic load, the BOD$_5$ removal rate decreased from 73.1% at $3.1m^3/m^2\cdot d$ to 65.3% at $9.2m^3/m^2\cdot d$. The design formula of this study which shows the removal rate of soluble BOD is $Le/Li =10^{-0.24} D/Q^{0.24}$ (Q: hydraulic load, D: depth of filter). 4. For experiment by organic load, the BOD$_5$ removal rate is increased from 70.2% at $0.77kg/m^3\cdot d$ to 75.4% at $4.28kg/m^3\cdot d$. We can obtain the straight line y=0.749 x (y: removed BOD, x :applied BOD) by the least squares method. 5. We can know that trickling filters is strong with the hydraulic load and the organic matter shock load. Here, we can judge that trickling filters is a good method for the treatment of swinery wastewater which containing high concentrated organic matter.

• Journal of Technologic Dentistry
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• v.23 no.2
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• pp.211-221
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• 2002

The purpose of this study was to analyze the commercial paste opaque products currently available in domestic of foreign as well as domestic, such as Duceram Plus(DU; Ducera Dental GmbH, Germany), VMK 95(VM; Vita Co., Germany), Noritake EX-3(EX; Noritake Co., Japan) and Ceramax(CE; Alphadent Co., Korea). They were characterized in thermal expansion coefficient, particle size distribution, viscosity and solvent using thermomechanical analyzer, particle size analyzer, rheometer and infrared spectrophotometer. Experimental results are as follows; Firstly, thermal expansion coefficients were determined $13.9{\times}10-6/^{\circ}C$ for DU, $14.3{\times}10-6/^{\circ}C$ for VM, $13.3{\times}10-6/^{\circ}C$ for EX, and $14.0{\times}10-6/^{\circ}C$ for CE. Secondly, percent of partice size below $1{\mu}m$ were 12% for DU, VM and CE, and 13% for EX, percent between $1{\mu}m$ and $5{\mu}m$ were 42% for DU, 42% for VM, 38% for EX, and 61 % for CE, percent between $5{\mu}m$ and $10{\mu}m$ were 21 % for DU, 24% for VM, 20% for EX, and 18% for CE, and over $10{\mu}m$ were 25% for DU, 22% for VM, 29% for EX, 9% for CE. Thirdly, the basic composition of the solvent in all of the commercial paste opaques were determined as ethylene glycol from FT-IR investigation. Lastly, measured viscosities were 1798 cp for DU, 536 cp for VM, 1110 cp for EX, and 721 cp for CE.

• Preventive Nutrition and Food Science
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• v.6 no.1
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• pp.43-50
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• 2001

The effects of cardiac ischemia-reperfusion and $\beta$-adrenergic stimulation on metabolic function and energetics were investigated in Lan gendorff-perfused spontaneously hypertensive rat (SHR) hearts. Sarcoplasmic reticulum {TEX}$Ca^{2+}${/TEX}-dependent ATPase and cardiac lactate dehydrogenase (LDH) are additionally studied. The perfusion medium (1.0 mM {TEX}$Ca^{2+}${/TEX}) contained 5 mM glucose(+5 U/L insulin) and 2 mM pyruvate as substrates. Global ischemia was induced by reducing perfusion pressure of 100 to 40 cm {TEX}$H_{2}${/TEX}O, followed by 20 min reperfusin. Isoproterenol (ISO, 1$\mu$M) was infused for 10 min. Coronary vascular resistance and myocardial oxygen consumption ({TEX}$MVO_{2}${/TEX}) of SHR were increased in parallel with enhanced venous lactate during ischemia and reperfusion compared to those of Sprague Dawley (SD) hearts. Although ischemia-induced increase in venous lactate and combined adenosine plus inosine was abolished, coronary vasodilation produced in SD during reperfusion. In SHR, depressed reactive hyperemia was associated with a fall in cardiac ATP and CrP/Pi ratio and a rise in intracellular lactate/Pyruvate ratio. On the other hand, ISO produced coronary functional hyperemia and an increase in {TEX}$MVO_{2}${/TEX}. However, these responses were less than those in SHR hearts. The ATPase activity of SHR was attenuated in free {TEX}$Ca^{2+}${/TEX} concentrations used under basal condition and with ISO compared to that of SD. Venous lactate output and cardiac LDH activity were augmented in SHR as influenced by ISO. These results demonstrate that coronary reactive and functional hyperemia was dpressed in SHR, which cold be explained by alterations in the cytosolic phosphorylation potential and the cytosolic redox state manipulated by LDH, and by abnormal free calcium handling.

• Journal of the Korean Crystal Growth and Crystal Technology
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• v.6 no.2
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• pp.263-274
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• 1996

Titanium dioxide ($TiO_{2}$) film has been widely used as anti-reflection coating for solar cells but not as masking oxide for metallisation and diffusion of impurities. In this paper we have investigated the properties of $TiO_{2}$ for possible incorporation into solar cell processing sequence. Thus the use of a spray deposition system to form the $TiO_{2}$ film and the characterisation of this film to ascertain its suitability to solar cell processing. The spray-on $TiO_{2}$ film was found to be resistant to all the chemicals used in conjunction with solar cell processing. The high temperature anealing (in oxygen ambient) of the spray-on $TiO_{2}$ film resulted in an increased refractive index, which indicated the growth of an underlying thin film of $SiO_{2}$ film for the passivation of silicon surface which would reduce the recombination activities of the fabricated device. Most importantly, the successful incorporation of the $TiO{2}$ film will lead to the reduction of the many high temperature processing steps of solar cell to only one.

• Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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• 2010.06a
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• pp.374-374
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• 2010

본 연구는 Roll-to-Roll Sputtering 장비를 사용하여 제작된 Flexible ITO electrode 필름의 방사선 검출기로의 적용가능성을 알아보기 위해 기존의 Glass ITO electrode의 전기적 특성을 비교 평가하였다. 본 연구는 Flexible ITO electrode와 Glass ITO electrode을 하부전극으로 형성하고, 최근에 X-ray 변환체로 활발히 연구되고 있는 Powder 형태의 반도체물질인 HgI2 와 PbI2를 Binder와 일정한 비율로 혼합하여 3-Rolls-Miller를 사용하여 Powder를 일정한 미세크기로 만들고, 대면적 제작이 용이한 Screen-Printing method을 이용하여 시편을 제작하였다. 제작된 필름은 하부전극의 종류에 따른 X-ray 입사 후의 전기적신호의 차이를 측정하고, HgI2와 PbI2 중 Flexible ITO electrode와 더욱 효율적으로 반응하여 기존의 Glass ITO electrode를 대체할 수 있는 전극을 발견하여 진단용 의료영상의 왜곡 현상을 제거할 수 있는 Flexible 방사선 검출기의 제작의 초석을 제공하는 연구를 목적으로 한다. SEM(Scanning Electron Microscope) 통하여 반도체 물질의 결정구조와 크기를 알아보았고, 하부 전극의 종류에 따른 전기적 신호검출을 위해 제작된 필름의 암전류(Dark current) 와 민감도(Sensitivity)를 측정한 후, SNR (Signal -to- Noise)을 계산하여 평가하였다.