• Title/Summary/Keyword: $Zn^{+2}/Fe^{+2}$ 이온

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Characteristics of Metallic and Ionic Concentration in $PM_{10}$ at Inland and Seashore in Busan (부산지역 내륙과 해안의 $PM_{10}$ 중의 금속농도와 이온농도 특성)

  • Jeon, Byung-Il;Hwang, Yong-Sik;Oh, Kwang-Joong
    • Journal of Environmental Impact Assessment
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    • v.19 no.3
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    • pp.323-333
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    • 2010
  • $PM_{10}$ mass were measured in Gwaebeopdong (inland) and Dongsamdong (seashore) of Busan in summer and fall, 2007 and the 24-hour averaged samples were analyzed to investigate temporal and spatial variability of metallic elements and water-soluble ions in $PM_{10}$. Overall average concentrations of $PM_{10}$ mass during the study period were 72.7 ${\mu}g/M^3$ and 64.3 ${\mu}g/M^3$ in Gwaebeopdong and Dongsamdong, respectively. As for metal elements, averaged concentrations of crustal components, Ca, Fe, K, Mn, and Ti, in Gwaebeopdong exhibited enhancement relative to Dongsamdong. Non-crustal elements, Pb and Cu, displayed elevated levels in Gwaebeopdong while Ni and Zn were observed to be high in Dongsamdong. Averaged nitrate concentration in Gwaebeopdong (6.36 ${\mu}g/M^3$) was greater than in Dongsamdong(5.68 ${\mu}g/M^3$) and both areas had higher level of nitrate in summer than in fall. Averaged sulfate concentrations in Dongsamdong (25.4%) exhibited elevated level relative to Gwaebeopdong (19.4%). Overall average contribution of water-soluble ions to $PM_{10}$ in Dongsamdong (47.5%) was higher than in Gwaebeopdong (37.8%). The average mass fractions of secondary ions in $PM_{10}$ were elevated in Dongsamdong (37.1%) as compared to Gwaebeopdong (31.4%). Equivalent ratio of [${SO_4}^{2-}/NO_3{^-}$] was seen to be lower in Gwaebeopdong (1.39) than that in Dongsamdong (1.79) and consistently higher in summer than in fall for both areas.

Asynchronous development of young gifted children by parents′ perception (부모의 지각에 따른 유아영재의 비동시적 발달특성)

  • 윤형주;윤여홍
    • Journal of Gifted/Talented Education
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    • v.13 no.1
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    • pp.65-80
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    • 2003
  • The purpose of this study was to investigate the Asynchronous development of young gifted children by parents' perception. Total 3 groups of 145 subjects from age 30 months to 6 years 10 months old young gifted children's parents participated. The major findings were as follows: (1) The mean developmental characteristics was at a high average level. The developmental subscales tended to be high. The level of verbal understanding/expression tended to be high. It reared as followed; intellectual capacity, emotional maturity, visual-motor coordination, morality, self-behavior control, emotion control, physical development, social development, peer relationship, leadership ability. (2) There were significant differences between intellectual capacity, verbal understanding /expression and physical, social development, self-behavior control, emotion control. There were significant differences between physical development, self-behavior control and emotion control as children got lower. There were significant differences between verbal understanding/expression and visual-motor coordination as children got older. There were significant differences between social development, peer relationship and self-behavior control, emotion control as children got older. Also, there were significant differences between leadership ability and self-behavior control, emotion control as children got older. There were significant differences between morality and self-behavior control as children got older. These findings suggested that young gifted children were in the special needs because of the developmental differences.

Purification and Characterization of Exo-xylanase from Escherichia coli Cells Harboring the Recombinant Plasmid pMGl (재조합 균주 Escherichia coli가 생산하는 Bacillus stearothermophilus Exo-xylanase의 정제 및 특성)

  • 문애란;최용진
    • Microbiology and Biotechnology Letters
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    • v.20 no.5
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    • pp.574-582
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    • 1992
  • Exo-xylanase encoded by the xylA gene of Bacillus stearothermoPhillus was produced from Escherichia coli ]M109 carrying a recombinant plasmid pMGL Synthesis of the enzyme was observed to be cell-associated, and about 94% of the enzyme synthesized was located in the cytoplasmic region. The maximum production was attained when the E. coli strain was grown at $37^{\circ}C$ for 8 hours on the medium containing 0.5% fructose, 1.0% tryptone, 1.0% sodium chloride, and 0.5% yeast extract. The exo-xylanase was purified to homogeneity using a combination of salting out with ammonium sulfate, DEAE-Sepharose CL-6B ion exchange chromatography, Sephadex G-IOO gel filtration, and Sephadex G-150 gel filtration. The' purified enzyme was most active at pH 6.0 and $45^{\circ}C$. $Ca^{2+}$ and $Co^{2+}$ activated the exo-xylanase activity by about 20% while $Ag^{2+}$, $Fe^{2+}$, $Mg^{2+}$ and $Zn^{2+}$ inhibited the enzyme activity by up to 60%. The $K_m$, value on p-nitrophenyl-$\beta$-D-xylanopyranoside was 2.75 mM. The enzyme had a pI value of 4.7. The estimated molecular weight of the native protein was 200,000 daL SDS-polyacrylamide gel electrophoresis analysis suggested that the native enzyme was a trimer composed of three identical 66,000 da!. polypeptides. The purified enzyme efficiently converted all the xylo-oligosaccharides tested to xylose. It was also confirmed that the enzyme split xylans in an exo-manner even though the degree of hydrolysis was fairly low. The xylanolytic enzyme was, therefore, classified to be one of the few bacterial exo-xylanases lacking transferase activity.

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Nutrient Contents of Bracken (Pteridium aquilinum L.) and Soil Chemical Properties of Its Habitat in the Coastal Area (남서해안 고사리 생육지의 토양화학적 특성과 고사리식물체의 무기성분 함량)

  • Lee, Soo-Young;Park, Kang-Yong;Park, Yang-Ho
    • Korean Journal of Soil Science and Fertilizer
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    • v.43 no.5
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    • pp.631-636
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    • 2010
  • One experiment was carried out to investigate the soil chemical properties of bracken growth and the inorganic element contents of plant. To the results of soil analysis in native bracken (Pteridium aquilinum L.) growth, soil pH was 5.2, organic matter was 19 g $kg^{-1}$ and available phosphate was 20 mg $kg^{-1}$, and exchangeable potassium, calcium, magnesium were 0.32, 2.0 and 1.3 $cmol_c\;kg^{-1}$, respectively. In the bracken cultivation soil, pH was 5.7, organic matter was 13 g $kg^{-1}$ and available phosphate 367 mg $kg^{-1}$, and exchangeable potassium, calcium and magnesium were 0.81, 4.0 and 1.4 $cmol_c\;kg^{-1}$, respectively. The soil pH, available phosphate and exchangeable calcium were much lower in bracken native soil than those of cultivation soil, while organic matter was a little higher in native soil than that of cultivation soil. In native bracken plants, three major elements of nitrogen, phosphorus and potassium, were 4.40, 0.55 and 3.40%, calcium and magnesium were 0.22 and 0.32%, and microelements of iron (Fe), manganese (Mn), copper (Cu), zinc (Zn) and boron were 126, 210, 23, 75 and 11 mg $kg^{-1}$, respectively. In cultivation bracken, three major elements of nitrogen, phosphorus and potassium, were 5.50, 0.73 and 3.55%, calcium and magnesium were 0.17 and 0.28%, and microelement contents of iron (Fe), manganese (Mn), copper (Cu), zinc (Zn) and boron (B) were 120, 252, 19, 72 and 20 mg $kg^{-1}$, respectively.

Food Components Characteristic of Oysters Produced in Korea (국내 산지별 굴의 성분 특성)

  • Choi, Jong-Duck;Hwang, Seok-Min;Kang, Jin-Young;Kim, Sang-Hyun;Kim, Jeong-Gyun;Oh, Kwang-Soo
    • Journal of agriculture & life science
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    • v.46 no.6
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    • pp.105-115
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    • 2012
  • The detailed proximate, fatty/amino acid, mineral composition, texture, color, chemical and taste compounds of six oysters (four kinds of cultured oysters and two kinds of wild oysters) in Korea were investigated. Length and weight of the shell removed cultured and wild oysters were 4.7~5.1 and 3.0~4.2 cm, and 5.9~9.1 and 2.6~5.5 g, respectively. The proximate compositions were not significantly different between cultured and wild oysters. Amino nitrogen and volatile basic nitrogen content of these ones were 232.8~258.2 and 160.5~213.9 mg/100 g, 9.5~12.0 and 7.8~9.5 mg/100 g, respectively. As a texture characteristic of muscle, shearing force were 95~114 and 105~132 g. Amounts of total amino acids of cultured and wild oysters were 9,004~10,198 and 8,165~8,942 mg/100 g, respectively. Major amino acids and inorganic ions were aspartic acid, glutamic acid, proline, alanine, leucine, phenylalanine, lysine, arginine and K, Na, Ca, Fe, S, P, Zn. Major fatty acids of these ones were 16:0, 18:0, 16:1n-9, 18:1n-9, 22:1n-9, 16:4n-3, 20:5n-3 and 22:6n-3, and there was little difference between the two groups. Amounts of free amino acids of cultured and wild oysters extracts were 1,444~1,620 and 1,017~1,277 mg/100 g, respectively, and major ones were taurine, glutamic acid, glycine, alanine, tryptophan, ornithine and lysine. There is a little difference in glycine, tryptophan, ornithine and arginine contents, but TMAO and TMA contents were low in amount, and were not significantly different between the two groups.

Impact of Application Rates of Pre-planting Liming Fertilizers on Changes in Soil Chemical Properties and Growth of 'Melody Yellow' Pansy in Plug Production (팬지 'Melody Yellow'의 플러그 육묘시 석회질 비료의 시비수준이 토양화학성 변화와 식물생장에 미치는 영향)

  • Lee, Poong-Ok;Lee, Jong-Suk;Choi, Jong-Myung
    • Horticultural Science & Technology
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    • v.28 no.5
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    • pp.735-742
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    • 2010
  • Objective of this research was to investigate the influence of liming fertilizers on changes in soil chemical properties and growth of pansy 'Melody Yellow' in plug production. To achieve this, dolomite (DO) with 0, 1.0, 3.5, 8.0 or $13.0g{\cdot}L^{-1}$ and calcium carbonate (CC) with 0, 2.0, 2.5, 3.0, 3.5, or $4.0g{\cdot}L^{-1}$ in application rate were incorporated into peatmoss + vermiculite (1:1, v/v) during the formulation. The treatments of $3.5g{\cdot}L^{-1}$ of DO and 2.5 or $3.0g{\cdot}L^{-1}$ of CC had acceptable range of soil solution pH such as 5.6-6.2. Faster rising of pH was observed in root media containing CC rather than those of DO, indicating higher solubility of CC. The soil Ca concentrations in all treatments of CC were 2 times as high as those of DO. The treatments of 3.5 or $8.0g{\cdot}L^{-1}$ of DO had the highest soil Mg concentrations, but all treatments of CC had lower soil Mg concentrations than control indicating that additional applications of Mg fertilizers are required. The elevated application rate of DO or CC resulted in the increase of fresh and dry weight; however, plant heights were not influenced by application of liming fertilizers. The results of tissue analysis showed that application of DO or CC influenced the Ca and Mg contents, but did not influence the contents of other nutrients such as N, P, Fe, Mn, Zn and Cu.

Removal of Red Tide Organisms -2. Flocculation of Red Tide Organisms by Using Loess- (적조생물의 구제 -2. 황토에 의한 적조생물의 응집제거-)

  • KIM Sung-Jae
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.33 no.5
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    • pp.455-462
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    • 2000
  • The objective of this study was to examine the physicochemical characteristics of coagulation reaction between loess and red tide organisms (RTO) and its feasibility, in developing a technology for the removal of RTO bloom in coastal sea. The physicochemical characteristics of loess were examined for a particle size distribution, surface characteristics by scanning electron microscope, zeta potential, and alkalinity and pH variations in sea water. Two kinds of RTO that were used in this study, Cylindrothen closterium and Skeietonema costatum, were sampled in Masan bay and were cultured in laboratory. Coagulation experiments were conducted using various concentrations of loess, RTO, and a jar tester. The supernatant and RTO culture solution were analyzed for pH, alkalinity, RTO cell number. A negative zeta potential of loess increased with increasing pH at $10^(-3)M$ NaCl solution and had -71.3 mV at pH 9.36. Loess had a positive zeta potential of +1,8 mV at pH 1.98, which resulted in a characteristic of material having an amphoteric surface charge. In NaCl and $CaCl_2$, solutions, loess had a decreasing negative zeta potential with increasing $Na^+\;and\;Ca^(+2)$ ion concentration and then didn't result in a charge reversal due to not occurring specific adsorption for $Na^+$ ion while resulted in a charge reversal due to occurring specific adsorption for $Ca^(+2)$ ion. In sea water, loess and RTO showed the similar zeta potential values of -112,1 and -9.2 mV, respectively and sea sand powder showed the highest zeta potential value of -25.7 mV in the clays. EDLs (electrical double-layers) of loess and RTO were extremely compressed due to high concentration of salts included in sea water, As a result, there didn't almost exist EDL repulsive force between loess and RTO approaching each other and then LVDW (London-yan der Waals) attractive force was always larger than EDL repulsive force to easily form a floe. Removal rates of RTO exponentially increased with increasing a loess concentration. The removal rates steeply increased until $800 mg/l$ of loess, and reached $100{\%}$ at 6,400 mg/l of loess. Removal rates of RTO exponentially increased with increasing a G-value. This indicated that mixing (i.e., collision among particles) was very important for a coagulation reaction. Loess showed the highest RTO removal rates in the clays.

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The Effect of Long-term Application of Different Organic Material Sources on Chemical Properties of Upland Soil (유기물원이 다른 퇴비연용이 밭토양의 화학성 변화에 미치는 영향)

  • Kim, Jong-Gu;Lee, Kyeong-Bo;Lee, Sang-Bok;Lee, Deog-Bae;Kim, Seong-Jo
    • Korean Journal of Soil Science and Fertilizer
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    • v.32 no.3
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    • pp.239-253
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    • 1999
  • The objective of this study was to determine the effects of various kinds of composts on the change of soil chemical properties in upland soils. Field experiments were conducted in the loam and sandy loam soils. Various kinds of composts such as poultry manure compost(PMC), cow manure compost(CMC), human excrement sludge(HES), and food industrial sludge compost(FISC) were applied annually at rates of 0, 40, and $80Mg\;ha^{-1}$ to soils grown with soybean and maize plants for 4 years during 1994 to 1997. The results of this study were as follows : The continuous application of human excrement sludge decreased soil pH up to 4.4~5.0, while other compost treatments increased soil pH compared with control plot. The EC increased initially and showed their maximum values at 20days after compost application, and then decreased up to 40 days, thereafter kept a certain level. The available phosphorous accumulated at 0~20cm depth in loam soil, and 0~50cm in sandy loam soil. Annual accumulation rates were 17% higher in sandy loam soil than loam soil. The more compost application rates and times, the higher base saturation percentage increased in upland soils. Four year's application at a rate of $80Mg\;ha^{-1}$ per year increased the base saturation percentage to 87~91% compared with 45% at control plot in the loam soil. While in sandy loam soil only three year's application of same rate increased the base saturation percentage to 81~92% compared with 30.4% at control plot. The average annual increasing rate of base saturation percentage at the same application rates of composts were higher in sandy loam soil by 2.0~3.7 times than in loam soil. The application of compost increased the exchangeable Ca, Mg, and K contents of soils by 2, 2~3, and 3~5 times, respectively, compared with the control. The contents of exchangeable canons were high in surface soil. and decreased with increase of soil depths. In the case of heavy metal content, there were no difference at the application of PMC and CMC but Ni. Fe, Zn, Cu was increased a little when the HES applied, and Ni and Cr was increased application with FISC.

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The Effect of Long-term Application of Different Organic Material Sources on Chemical Properties of Upland Soil (유기물원이 다른 퇴비연용이 밭토양의 화학성 변화에 미치는 영향)

  • Kim, Jong-Gu;Lee, Kyeong-Bo;Lee, Sang-Bok;Lee, Deog-Bae;Kim, Seong-Jo
    • Korean Journal of Soil Science and Fertilizer
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    • v.33 no.6
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    • pp.416-431
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    • 2000
  • The objective of this study was to determine the effects of various kinds of composts on the change of soil chemical properties in upland soils. Field experiments were conducted in the loam and sandy loam soils. Various kinds of composts such as poultry manure compost(PMC), cow manure compost(CMC), human excrement sludge(HES), and food industrial sludge compost(FISC) were applied annually at rates of 0, 40, and $80Mg\;ha^{-1}$ to soils grown with soybean and maize plants for 4 years during 1994 to 1997. The results of this study were as follows : The continuous application of human excrement sludge decreased soil pH up to 4.4~5.0, while other compost treatments increased soil pH compared with control plot. The EC increased initially and showed their maximum values at 20days after compost application, and then decreased up to 40 days, thereafter kept a certain level. The available phosphorous accumulated at 0~20cm depth in loam soil, and 0~50cm in sandy loam soil. Annual accumulation rates were 17% higher in sandy loam soil than loam soil. The more compost application rates and times, the higher base saturation percentage increased in upland soils. Four year's application at a rate of $80Mg\;ha^{-1}$ per year increased the base saturation percentage to 87~97% compared with 45% at control plot in the loam soil. While in sandy loam soil only three year's application of same rate increased the base saturation percentage to 81~92% compared with 30.4% at control plot. The average annual increasing rate of base saturation percentage at the same application rates of composts were higher in sandy loam soil by 2.0~3.7 times than in loam soil. The application of compost increased the exchangeable Ca, Mg, and K contents of soils by 2, 2~3, and 3~5 times, respectively, compared with the control. The contents of exchangeable cations were high in surface soil, and decreased with increase of soil depths. In the case of heavy metal content, there were no difference at the application of PMC and CMC but Ni, Fe, Zn, Cu was increased a little when the HES applied, and Ni and Cr was increased application with FISC.

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${\beta}$-1,4-Xylosidase Activity of Leuconostoc Lactic Acid Bacteria Isolated from Kimchi (김치에서 분리된 Leuconostoc 속 젖산균의 ${\beta}$-1,4-xylosidase 효소생산 특성)

  • Jang, Mi-Hee;Kim, Myoung-Dong
    • Korean Journal of Food Science and Technology
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    • v.43 no.2
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    • pp.169-175
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    • 2011
  • The ${\beta}$-xylosidase (EC 3.2.1.37) production capabilities of lactic acid bacteria in the genus Leuconostoc, isolated from a variety of kimchi (fermented vegetables), were examined. The intracellular levels of ${\beta}$-xylosidase were similar to the extracellular levels, when most Leuconostoc lactic acid bacteria were grown in a medium containing xylose as the carbon source. Intracellular ${\beta}$-xylosidase with a maximum activity of $1.2{\pm}0.1units/mL$ (mean${\pm}$standard error) was obtained from Leuconostoc lactis KCTC 13344, which was isolated from fermented Chinese cabbage. The optimum reaction conditions for Leu. lactis KCTC 13344 ${\beta}$-xylosidase activity were pH 6.0 and $30^{\circ}C$, and the addition of most divalent cations, except zinc, to the reaction mixture resulted in a slight increase in enzyme activity. Compared with a media containing other carbon sources, the ${\beta}$-xylosidase activity was 5 times higher when Leu. lactis KCTC 13344 was grown in a medium containing xylose as carbon source. Zymographic analysis indicated that the synthesis of Leu. lactis KCTC 13344 ${\beta}$-xylosidase (approximate size, 64 kDa) is induced by xylose. A maximum intracellular ${\beta}$-xylosidase activity of $7.1{\pm}0.3units/mL$ was obtained in a batch cultivation in an MRS medium containing 30 g/L xylose.