• Title/Summary/Keyword: $V_{S30}$

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Studies on the Film forming Yeasts Isolated from Commercial Soy Sauce (제품(製品)간장에서 분리(分離)한 산막효모(産膜酵母)에 관(關)한 연구(硏究))

  • Chu, Young-Ha;Yu, Tai-Jong;Yu, Ju-Hyun
    • Korean Journal of Food Science and Technology
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    • v.7 no.2
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    • pp.61-68
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    • 1975
  • This study was conducted (1) to isolate the film-forming yeast from the commercially available soy sauce, (2) to identify the state of soy sauce fermentation by the use of yeasts, (3) to confirm characteristics of yeasts. The result were as follows. 1. These yeast strains in the soy sauce fermentation test showed full fermentation of whole sugar content, reduction of the pure extract and relative reduction in total nitrogen. Soy sauce color was somehow faded to lower the stability of soy sauce. 2. In anti-fungal activity test butylparaben at a higher level 60 ppm., sodium propionate 2,400 ppm, sodium benzoate 800 ppm., menadion 165 ppm, showed their anti-fungal effect, while alcohol did not show the effect in the 3% additive group. 3. The optimum sodium chloride concentration for these strains in the 2% G.Y.P. medium was 5% and optimum temperature was $30^{\circ}C$. The extinction temperature was $62^{\circ}C$ for strain No-1 and No-3, and was $65^{\circ}C$ for No-2 and No-4. 4. The film-forming soy sauce turned out in the gas chromatogram to possess much flavor of low boiling point as compared with the standard. These flavors were considered due to flavor spoilage of the soy sauce. 5. These isolated yeasts were identified Saccharomyces rouxii (film-forming yeast) in the Lodder's taxanomic study.

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Flora and Phytogeography on Mt. Deokhang (Gangwon-do), Limestone area in Korea (석회암지대인 덕항산 (강원)의 관속식물상과 식물지리)

  • Kim, Jin-Seok;Lee, Byeng-Cheon;Chung, Jae-Min;Pak, Jae-Hong
    • Korean Journal of Plant Taxonomy
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    • v.35 no.4
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    • pp.337-364
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    • 2005
  • The vascular flora of Mt. Deokhang (a.s.l. 1072.5m, $37^{\circ}17^{\prime}{\sim}37^{\circ}20^{\prime}N$, $128^{\circ}09^{\prime}{\sim}129^{\circ}03^{\prime}E$), one of the limestone areas in Korea and is located between Samcheok-si and Taebaek-si, Gangwon-do, is surveyed from 10 Oct. 2003 to 4 Oct. 2005. The characteristics of the flora from the phytogeographical point of view is also discussed. The number of the vascular plant taxa identified in this study is 590 in total which belongs to 90 families, 337 genera, 537 species, 4 subspecies, 46 varieties and 3 forms. Of them, 30 taxa are endemic to Korea, 4 taxa are legally protected ones, and 17 taxa are generally regarded as rare and endangered species in Korea. Naturalized plants are 19 taxa. The phytogeographical importance and the conservation strategy of the flora of Mt. Deokhang focusing on either rare, endemic or phytogeographically important species are discussed.

The Effect of Korean Ganoderma Lucidum Extract on the Fermentation of Lactose-Fermentation Yeast (영지(靈芝) (Ganoderma lucidum) 추출물(抽出物)이 유당(乳糖) 발효성(醱酵性) 효모(酵母)의 발효(醱酵)에 미치는 영향(影響))

  • Kim, Jong Woo;Kim, Ine Tae
    • Korean Journal of Agricultural Science
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    • v.22 no.1
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    • pp.54-61
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    • 1995
  • In order to investigate the effects of Ganoderma lucidum on the fermentation of media by Kluyveromyces fragilis KCCM 35458, with 0%, 0.1%, 0.5%, and 1.0% (w/v) of Ganoderma lucidum extract were added to skin milk and fermented in the suspension culture at $30^{\circ}C$ for 72 hours. The pH, acidity, $CO_2$ evolution, alcohol production, number of yeast cells and lactose content were investigated. The results are summarized as follows; 1. As fermentation time advanced by the pH was decreased and the acidity was increased in the media of 1.0% Gnaoderma lucidum extract as fermentation time advanced. 2. $CO_2$ evolution was increased in the all of Genoderma lucidum extract and significantly effect showed in 1.0% extract medium. 3. During 72 hours culturing, alcohol was detected in all Gandoderma lucidum extract media, 1.0% Ganoderma lucidum extract medium showed extremly high in alcohol and shortened fermentation time. 4. The number of yeast was increased more at logarithmic phase and the growth of yeast was more rapid in all Ganoderma lucidum extract especially 0.5%, 1.0% media samples than control. 5. As fermentation time goes by, the lactose content of media were decreased more rapidly in 1.0% Ganoderma lucidum extract medium than control.

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Studies on Cheese Ripening Part V. Degradation of Nitrogenous Compounds During the Ripening of Gouda-type Cheese (치이즈 숙성(熟成)에 관(關)한 연구(硏究) 제5보(第5報) Gouda-type 치이즈의 질소화합물(窒素化合物)의 변화(變化))

  • Kim, Yong-Kyo;Kim, Jong-Shik
    • Korean Journal of Food Science and Technology
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    • v.8 no.2
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    • pp.107-112
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    • 1976
  • This experiment has been carried out to study the changes of caseins is Gouda-type cheese during ripening by the use of DEAE-cellulose column and polyacrylamide gel electrophoresis. The results obtained were as follow: 1. The amount of water soluble nitrogenous compounds in the cheese ripened for 2 and 4 months was 19.03% and 30.61% for total nitrogen in the cheese, respectively. On the other hand, the amount of 5% NaCl soluble nitrogenous compounds in the cheese increased to 41.13% for 2 months ripening, but it decreased to 22.61% at 4th month. 2. By DEAE-cellulose column, various nitrogenous compounds of Gouda-type cheese were fractionated into 5 major and several minor peaks. The proportion of some fraction areas to the whole increased with the ripening period and the others decreased. 3. Electrophoretic patterns of various nitrogenous compounds in a 4-month-old cheese showed 5 and 8 bands, repectively. 4. Sixteen amino acids were identified in soluble and precipitable compounds at 12% TCA in a 4-month-old cheese.

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Effects of Constitutional Food on Nurse's NK Cell Activity and Stress Reduction (체질푸드가 간호사의 스트레스 감소와 NK세포 활성도에 미치는 영향)

  • Park, Sun-Mi
    • The Journal of the Korea Contents Association
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    • v.19 no.3
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    • pp.500-509
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    • 2019
  • The study looked at the effect of body food on stress reduction and NK cell activity in improving natural healing for nurses, and examined whether it is effective in preventing and curing human health. The study was conducted for 30 days on 22 nurses who currently worked for more than a year at a general hospital in Gyeonggi Province. Stress measurements were conducted through pulse wave measuring instrument, and blood tests were conducted on the activity of NK cells. Treatment of collected data was performed by Paired t-testing through the Stat program SPSS 21 and providing body food suitable for the physical quality of participants after the classification of the body based on the internal diameter of the emperor and scholarship. Studies have shown that the effect on reducing the stress of body food has significant positive effects and that body food is effective for the activity of NK cells. This study has the following significance: First, the main health threat factor for modern people is the increased resistance of natural healing through body food to stress, which has the potential to prevent disease. Second, immunodeficiency in disease prevention and treatment is very important, which can increase the natural healing power of the human body by increasing the activity of NK cells through body food.

Gestational Exposure to Pesticides Induces Oxidative Stress and Lipid Peroxidation in Offspring that Persist at Adult Age in an Animal Model

  • Ndonwi, Elvis Ngwa;Atogho-Tiedeu, Barbara;Lontchi-Yimagou, Eric;Shinkafi, Tijjani S.;Nanfa, Dieudonne;Balti, Eric V.;Indusmita, Routray;Mahmood, Amena;Katte, Jean-Claude;Mbanya, Armand;Matsha, Tandi;Mbanya, Jean Claude;Shakir, Ali;Sobngwi, Eugene
    • Toxicological Research
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    • v.35 no.3
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    • pp.241-248
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    • 2019
  • Pesticide exposure may induce biochemical alterations including oxidative stress and lipid peroxidation. However, in the context of developmental origin of health and disease, putative trans-generational effect of exposure to pesticides are insufficiently studied. We therefore aimed to evaluate the biochemical effect of gestational exposure to four pesticides on female Wistar rats and their offspring at adult age. We studied 30 female nulliparous Wistar rats divided into 5 equal groups. Group 1 served as the control group and received distilled water while group 2, 3, 4 and 5 received orally pesticide 1 (imidacloprid), pesticide 2 (chlorpyrifos), pesticide 3 (imidacloprid + lambda cyhalothrin) and pesticide 4 (oxamyl) respectively once daily throughout gestation at a dose equivalent to 1/10 lethal dose 50. The mothers were followed up until one month post gestation. The offspring were followed up from birth until adult age (12 weeks). In all animals at each time point we evaluated malondialdehyde (MDA), oxidative stress and liver function enzymes. There was similar variation of total body weight in all the groups during and after gestation. However, Female Wistar rats of the exposed groups had significant alterations in liver SOD (-30.8% to +64.1%), catalase (-38.8% to -85.7%) and GSH (-29.2% to -86.5%) and; kidney catalase (> 100%), GSH (> 100%). Moreover, MDA, alanine transaminase (ALT) and aspartate transaminase (AST) levels were significantly higher in pesticide exposed rats compared to the control group. Similar alterations in antioxidant enzymes, MDA and liver function enzymes were observed in offspring of treated rats evidenced at weaning and persisting until adult age. Exposure to pesticides causes oxidative stress and lipid peroxidation in exposed female Wistar rats and their offspring. The persistence in offspring at adult age suggests transgenerational adverse effects.

Development of an apple/pear pomace fermented with Lentinus edodes Mycelia (사과/배 부산물 및 표고버섯균사체를 이용한 발효물 개발)

  • Kim, Jin-Kyeong;Jo, Seong-Wha;Kim, Eun-Ji;Jeong, Do-Youn
    • Korean Journal of Food Science and Technology
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    • v.51 no.3
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    • pp.286-294
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    • 2019
  • The purpose of this study was to investigate the possibility of enhancing the functional compounds in apple and pear pomace (APP) by fermentation with mycelia from the mushroom Lentinula edodes. A 30% (w/v) APP with added rice bran and Biji was fermented with L. edodes at $24^{\circ}C$ and 80% humidity. The cellulase and pectinase activities in the fermented APP (FAPP) were higher than those in the non-fermented control. In addition, the physiological activities of the FAPP, including DPPH, ABTS radical scavenging, and SOD-like activity, as well as the total polyphenol and ${\beta}-glucan$ contents were higher than those in the control. FAPP treatment significantly reduced LPS-induced nitric oxide (NO) levels in Raw 264.7 cell. Therefore, FAPP treatment was considered to more effectively suppress cell injury caused by inflammatory cytokines through inhibition of LPS-induced NO production. These results suggest that the levels of functional components in APP can be increased by fermentation with this mushroom mycelium. However, further studies are needed before it can be used as a functional material.

Phosphorescent Organic Light Emitting Diodes using the Emission Layer of (TCTA/$TCTA_{1/3}TAZ_{2/3}/TAZ):Ir(ppy)_3$ ((TCTA/$TCTA_{1/3}TAZ_{2/3}/TAZ):Ir(ppy)_3$ 발광층을 이용한 녹색 인광소자)

  • Jang, J.G.;Shin, S.B.;Shin, H.K.;Kim, W.K.;Ryu, S.O.;Chang, H.J.;Gong, M.S.;Lee, J.Y.
    • Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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    • 2008.04a
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    • pp.33-35
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    • 2008
  • We have fabricated and evaluated new high efficiency green light emitting phosphorescent devices with an emission layer of $[TCTA_{1/3}TAZ_{2/3}/TAZ]:Ir(ppy)_3$. The whole experimental devices have the basic structure of $2-TNATA(500 {\AA})/NPB(300{\AA})/EML(300{\AA})/BCP(50{\AA})/SFC137(500{\AA})$ between anode and cathode. We have also fabricated conventional phosphorescent devices with emission layers of $(TCTA_{1/3}TAZ_{2/3}):Ir(ppy)_3$ and $(TCTA/TAZ):Ir(ppy)_3$ and compared their electroluminescence characteristics with those of the device with an emission layer of $(TCTA/TCTA_{1/3}TAZ_{2/3}/TAZ):Ir(ppy)_3$. The current density(J), luminance(L), and current efficiency($\eta$) of the device with an emission layer of $(80{\AA}-TCTA/90{\AA}-TCTA_{1/3}TAZ_{2/3}/130{\AA}-TAZ):10%-Ir(ppy)_3$ were 95 $mA/cm^2$, 25000 $cd/m^2$, and 27 cd/A at an applied voltage of 10V, respectively. The maximum current efficiency was 52 cd/A under the luminance of 400 $cd/m^2$. The peak wavelength and FWHM(full width at half maximum) in the electroluminescence spectral were 513nm and 65nm, respectively. The color coordinate was (0.30, 0.62) on the CIE (Commission Internationale de l'Eclairage) chart. Under the luminance of 15000 $cd/m^2$, the current efficiency of the device with an emission layer of $(80{\AA}-TCTA/90{\AA}-TCTA_{1/3}TAZ_{2/3}/130{\AA}-TAZ):10%-Ir(ppy)_3$ was 34 cd/A, which has been improved 1.7 times and 1.4 limes compared to those of the devices with emission layers of $(300{\AA}-TCTA_{1/3}TAZ_{2/3}): 10%-Ir(ppy)_3$ and $(100{\AA}-TCTA/200{\AA}-TAZ):10%-Ir(ppy)_3$, respectively.

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Purification and Characterization of Cytochrome c Oxidase from Photosynthetic Bacterium, Rhodopseudomonas gelatinosa (광합성세균 Rhodopseudomonas gelatinosa 의 시토크롬 c 산화효소의 정제 및 특성)

  • 강대길;최원기
    • Korean Journal of Microbiology
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    • v.30 no.2
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    • pp.101-107
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    • 1992
  • Cytochrome c oxida5e from chemotrophically grown R p , geliitinosu was purified by cytochrome c affinity chromatography and DEAE-Sephacel ion exchange chromatography. The molecular weight of the cytochrome c oxidase was approximately 110.000 Da by sephacryl s-300 gel chromatography and approximately 52, 000 Da by SDS-gel electrophoresis, respectively. Therefore. cytochrolne c oxidase of Rps. gehtinosu seems to be dimer. The cytochrome c oxidasc was very sensitive to temperature. It's Km and Vmax were 20 pM and 44 unitlmg protein for horsc heart cytochrome c as a substrate. respectively, and its optimum pH and temperature were 6.4 and 25$^{\circ}$C. respectively. The absorption peaks of the reduced cytochrome c oxidase showed at 554 nm, 523 nm. and 422 nm. The activiiy of cytochrome c oxidase was inhibited by KCN, and NaN3, but not by CO, antimycir~ A. and myxothiazol. The cytochrome c-551 was produced either in phototrophically or chemotrophically grown Rps. gelaiinosci. The rcduced cytochrome c-551 was oxidized by b-type cytochrome c oxidase from Rp.v. gc.lrtino.sc~. Km and Vmax of cytochrome c oxidase was 26 pM and 31 unitlnlg protein For cytochrome c-551 as a substrate. respectively. Thercfore. thc electron transfer chain of chemotrophically grown Rps. glatinosa seems lo be ubiquinol cytochrome bc, complex -'cytochrome c-55lMb-type cytochrome c oxidase+02.

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Influence of Consumption Patterns and Satisfaction for Convenience Store Private Brand Foods (편의점 PB식품군 구매행동 및 만족도 영향요인 연구)

  • Kim, Min-Jeong
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.17 no.3
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    • pp.629-637
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    • 2016
  • This study examined the purchasing behavior and satisfaction of people with convenience store PB foods. The factors affecting the purchasing behavior and the overall satisfaction with the convenience store PB foods were examined. This study first found that the purchasing motivation of PB foods is the cheaper price than NB foods. Second, age is a statistically effective variable for the visit frequency at the convenience store and gender and age are effective variables for the purchase frequency of PB foods. Third, there is a statistically positive correlation of convenience store brand preference with the PB food brand preference for 6 food categories. Next, in terms of the attribute-level satisfaction, the most satisfied areas are diverse food groups and diverse food taste and the least satisfied areas is price. This is followed by taste and design/packaging influence overall satisfaction. From this analysis, the following suggestions were drawn. First, because consumers are likely to be matched convenience store brand preference and PB food brand preference, advertising is necessary to ensure that the consumers continue to visit the convenience store. Second, product improvement by increasing the quality should be designed to motivate the major consumer group of people in their 20s and 30s. Finally, to boost PB food satisfaction, the industry should improve the taste and design/packaging.