• The Journal of Advanced Prosthodontics
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• 제7권3호
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• pp.183-190
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• 2015

PURPOSE. This study evaluated the fracture load of customized zirconia abutments with titanium insert according to preparation depths, with or without 5-year artificial aging. MATERIALS AND METHODS. Thirty-six identical lithium disilicate crowns (IPS e.max press) were fabricated to replace a maxillary right central incisor and cemented to the customized zirconia abutment with titanium insert on a $4.5{\times}10$ mm titanium fixture. Abutments were fabricated with 3 preparation depths (0.5 mm, 0.7 mm, and 0.9 mm). Half of the samples were then processed using thermocycling (temperature: $5-55^{\circ}C$, dwelling time: 120s) and chewing simulation (1,200,000 cycles, 49 N load). All specimens were classified into 6 groups depending on the preparation depth and artificial aging (non-artificial aging groups: N5, N7, N9; artificial aging groups: A5, A7, A9). Static load was applied at 135 degrees to the implant axis in a universal testing machine. Statistical analyses of the results were performed using 1-way ANOVA, 2-way ANOVA, independent t-test and multiple linear regression. RESULTS. The fracture loads were $539.28{\pm}63.11$ N (N5), $406.56{\pm}28.94$ N (N7), $366.66{\pm}30.19$ N (N9), $392.61{\pm}50.57$ N (A5), $317.94{\pm}30.05$ N (A7), and $292.74{\pm}37.15$ N (A9). The fracture load of group N5 was significantly higher than those of group N7 and N9 (P<.017). Consequently, the fracture load of group A5 was also significantly higher than those of group A7 and A9 (P<.05). After artificial aging, the fracture load was significantly decreased in all groups with various preparation depths (P<.05). CONCLUSION. The fracture load of a single anterior implant restored with lithium disilicate crown on zirconia abutment with titanium insert differed depending on the preparation depths. After 5-year artificial aging, the fracture loads of all preparation groups decreased significantly.

• 대한토목학회논문집
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• 제35권4호
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• pp.863-874
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• 2015

본 연구에서는 보강날개로 보강된 수중잠제 지지말뚝의 보강효과를 분석하기 위하여 하중전이거동을 분석하였다. 먼저 3차원 유한요소해석을 통해 말뚝의 변위 및 하중전이곡선(지반반력) 결과로부터 보강효과를 확인하고, 하중전이곡선에서 극한저항력 비율로 보강말뚝의 수평 및 인발하중에 대한 보강계수를 산정함으로써 보강효과를 정량화 하였다. 산정된 보강계수를 쌍곡선 하중전이함수의 극한저항력 $p_u$와 $t_{max}$에 곱해서 보정상수(fitting parameter)로 적용하여 보강효과를 고려한 하중전이함수를 제안하였다. 제안된 하중전이함수가 수치해석 결과를 잘 반영하고 있는지 확인하기 위해 3차원 유한요소해석을 비교하여 보강말뚝의 하중전이거동을 분석하였다. 제안된 하중전이함수를 하중전이법 해석에 적용하여, 실제 수중잠제를 해석한 결과 보강말뚝의 전단력, 휨 모멘트, 변위가 무보강말뚝보다 작게 발생하고, 지반반력은 더 크게 발생하여 비교적 경제적인 설계가 가능할 것으로 판단된다.

• Biomolecules & Therapeutics
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• 제18권4호
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• pp.469-476
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• 2010

This study was to investigate the effect of apigenin on the bioavailability of paclitaxel after oral and intravenous administration in rats. The effect of apigenin on P-glycoprotein (P-gp), cytochrome P450 (CYP)3A4 activity was evaluated. The pharmacokinetic parameters of paclitaxel were determined in rats after oral (40 mg/kg) or intravenous (5 mg/kg) administration of paclitaxel with apigenin (0.4, 2 and 8 mg/kg) to rats. Apigenin inhibited CYP3A4 activity with 50% inhibition concentration ($IC_{50}$) of 1.8 ${\mu}M$. In addition, apigenin significantly inhibited P-gp activity. Compared to the control group, apigenin significantly increased the area under the plasma concentration-time curve (AUC, p<0.05 by 2 mg/kg, 59.0% higher; p<0.01 by 8 mg/kg, 87% higher) of oral paclitaxel. Apigenin also significantly (p<0.05 by 2 mg/kg, 37.2% higher; p<0.01 by 8 mg/kg, 59.3% higher) increased the peak plasma concentration ($C_{max}$) of oral paclitaxel. Apigenin significantly increased the terminal half-life ($t_{1/2}$, p<0.05 by 8 mg/kg, 34.5%) of oral paclitaxel. Consequently, the absolute bioavailability (A.B.) of paclitaxel was significantly (p<0.05 by 2 mg/kg, p<0.01 by 8 mg/kg) increased by apigenin compared to that in the control group, and the relative bioavailability (R.B.) of oral paclitaxel was increased by 1.14- to 1.87-fold. The pharmacokinetics of intravenous paclitaxel were not affected by the concurrent use of apigenin in contrast to the oral administration of paclitaxel. Accordingly, the enhanced oral bioavailability by apigenin may be mainly due to increased intestinal absorption caused via P-gp inhibition by apigenin rather than to reduced renal and hepatic elimination of paclitaxel. The increase in the oral bioavailability might be mainly attributed to enhanced absorption in the gastrointestinal tract via the inhibition of P-gp and reduced first-pass metabolism of paclitaxel via the inhibition of the CYP3A subfamily in the small intestine and/or in the liver by apigenin. It appears that the development of oral paclitaxel preparations as a combination therapy is possible, which will be more convenient than the i.v. dosage form.

• 한국진공학회:학술대회논문집
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• 한국진공학회 2013년도 제44회 동계 정기학술대회 초록집
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• pp.176-177
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• 2013

Surface plasmon polaritons (SPPs) have attracted the attention of scientists and engineers involved in a wide area of research, microscopy, diagnostics and sensing. SPPs are waves that propagate along the surface of a conductor, usually metals. These are essentially light waves that are trapped on the surface because of their interaction with the free electrons of conductor. In this interaction, the free electrons respond collectively by oscillating in resonance with the light wave. The resonant interaction between the surface charge oscillation and the electromagnetic field of the light constitutes the SPPs and gives rise to its unique properties. In this papers, we studied theoretical and experimental extraordinary transmittance (T) and reflectance (R) of 2 dimensional metal hole array (2D-MHA) on GaAs in consideration of the diffraction orders. The 2d-MHAs was fabricated using ultra-violet photolithography, electron-beam evaporation and standard lift-off process with pitches ranging from 1.8 to $3.2{\mu}m$ and diameter of half of pitch, and was deposited 5-nm thick layer of titanium (Ti) as an adhesion layer and 50-nm thick layer of gold (Au) on the semiinsulating GaAs substrate. We employed both the commercial software (CST Microwave Studio: Computer Simulation Technology GmbH, Darmstadt, Germany) based on a finite integration technique (FIT) and a rigorous coupled wave analysis (RCWA) to calculate transmittance and reflectance. The transmittance was measured at a normal incident, and the reflectance was measured at variable incident angle of range between $30^{\circ}{\sim}80^{\circ}$ with a Nicolet Fourier transmission infrared (FTIR) spectrometer with a KBr beam splitter and a MCT detector. For MHAs of pitch (P), the peaks ${\lambda}$ max in the normal incidence transmittance spectra can be indentified approximately from SP dispersion relation, that is frequency-dependent SP wave vector (ksp). Shown in Fig. 1 is the transmission of P=2.2 um sample at normal incidence. We attribute the observation to be a result of FTIR system may be able to collect the transmitted light with higher diffraction order than 0th order. This is confirmed by calculations: for the MHAs, diffraction efficiency in (0, 0) diffracted orders is lower than in the (${\pm}x$, ${\pm}y$) diffracted orders. To further investigate the result, we calculated the angular dependent transmission of P=2.2 um sample (Fig. 2). The incident angle varies from 30o to 70o with a 10o increment. We also found the splitting character on reflectance measurement. The splitting effect is considered a results of SPPs assisted diffraction process by oblique incidence.

• Journal of Pharmaceutical Investigation
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• 제39권1호
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• pp.73-78
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• 2009

A simple LC-MS/MS method of rabeprazole in human plasma was developed and validated. Rabeprazole and Internal standard (I.S), omeprazole, were extracted from human plasma by liquid liquid extraction, chromatographic separation of rabaprazole in plasma was achieved at $45^{\circ}C$ with a Shiseido UG120 $C_{18}$ column and methanol-10 mM ammonium acetate buffer (pH 9.42 with ammonium water), as mobile phase. Rabeprazole produced a protonated precursor ion [$(M+H)^+$] at m/z 360.10 and corresponding product ion at m/z 242.21. Internal standard produced a protonated precursor ion [$(M+H)^+$] at 346.09 and corresponding product ion at m/z 198.09. This method showed linear response over the concentration range of $1{\sim}500\;ng/mL$ with correalation coefficient greater than 0.99. The lower limit of quantitation (LLOQ) using 0.2 mL plasma was 1 ng/mL, which was sensitive enough for pharmacokinetics studies. The method was specific and validated with a limit of quantitation of 1 ng/mL. The intra-day and inter-day precision and accuracy were acceptable for all samples including the LLOQ. The applicability of the method was demonstrated by analysis of plasma after administration of a single 10 mg dose to 36 healthy subject. From the plasma rabeprazole concentration versus time curves, the mean $AUC_t$ (The area under the plasma concentration-time curve from time 0 to 12 hr ) was $691.36{\pm}321.88\;ng{\cdot}hr/mL$, $C_{max}$ (maximum plasma drug concentration) of $353.21{\pm}131.52\;ng/mL$ reached $3.4{\pm}1.1\;hr$ after adiministration. The mean biological half-life of rabeprazole was $1.37{\pm}0.75\;hr$. Based on the results, this simple method could readily be used in pharmacokinetics studies.

• 수산해양기술연구
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• 제38권2호
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• pp.110-118
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• 2002

본 논문에서는 무요소 이론을 정식화하였고 이를 이용한 1차원 및 2차원 EFG 프로그램을 Visual Basic과 C언어를 이용하여 작성해 보았다. 그리고 각각의 EFG 수치해석의 예를 작성된 프로그램을 이용하여 해를 구하였다. 해석결과는 다른 문헌의 결과와 일치하였으며 해석결과에서 나타나듯이 무요소 해의 정도는 영향영역의 비례축소인자 dmax와 가중함수의 종류, 절점 배치형태에 의해 좌우된다는 사실을 알 수 있었다. 특히 1, 2차원 EFG 해석결과에서 가장 최적의 해를 보이며 정해(exact solution)에 가장 근접한 조건은 dmax = 2 이고 가중함수가 3차 Spline형일 때로 나타났으며 유한요소법과 마찬가지로 절점의 수가 많을 수록 그리고 절점을 균일하게 배치할수록 높은 정도를 나타내는 것을 알 수 있었다. 특히 2차원의 경우 3차 Spline형 이외의 다른 가중함수를 사용할 경우에 상당히 큰 오차를 나타내는 점은 1차원 EFG 해석의 결과와는 다른 점이었지만 그 외 대부분 같은 결과를 나타내었다. 1차원에서 절점을 임의로 배치한 경우는 비교적 균일하게 배치한 경우가 해에 근접하는 형태를 나타내었으며 절점 간격이 상대적으로 적은 곳에서 큰 오차를 나타내었다. 그리고 절점을 임의로 선택할 때 변위가 모두 ‘0’의 값을 가지는 경우를 볼 수 있는데, 이는 화면상의 좌표계산에서 생긴 미소한 오차가 절점들에 의해 반복됨으로서 발생하는 것으로 보인다. 또한 탄성계수 값이 클 경우 dmax 에서 계산이 제대로 수행되지 못하는 경우가 있는데, 이는 수치가 double형의 크기를 초과하기 때문인 것으로 보인다. 결과에서 나타나듯이 무요소법에서 적당한 가중함수와 비례축소 인자를 사용하면 정해에 가까운 우수한 해를 얻을 수 있다는 것을 알 수 있다. 비록 프로그래밍 과정이나 이론의 정식화가 유한요소법에 비해 상당히 어려운 점은 있으나 무요소법은 요소의 정보를 필요치 않으므로 사용자 입장에서는 매우 편리할 것이다. 앞으로 경계조건을 효과적으로 만족시키는 문제를 해결하고 효과적인 알고리즘이 개발된다면 실용적으로 유한요소법을 대신할 수 있는 좋은 대안이 될 수 있을 것이라 생각된다.

• 한국균학회지
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• 제25권4호통권83호
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• pp.330-339
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• 1997

식물 병원진균 Botrytis cinerea가 분비하는 polygalacturonase의 동위효소 중에서 endo-type의 polygalacturonase를 Phenyl-Toyopearl column chromatography와 DEAE-, Mono Q-, heparin-high performance liquid chromatography법으로 약 68배 정제하였고 sodium dodecylsulfate(SDS)를 포함하는 polyacrylamide gel electrophoresis로 분석하여 단일 띠를 확인하였다. 정제한 효소의 분자량은 SDS-polyacrylamide gel에서 37 kDa로 측정되었으며 약산성인 조건과 $30^{\circ}C$ 이하에서 안정하였으며 최고 활성은 pH 4.5와 $55^{\circ}C$의 온도에서 관찰되었다. 정제한 효소는 polygalacturonic acid를 endo-type으로 가수분해하였고, polygalacturonic acid에 대한 $K_m$값과 $V_{max}$값은 각각 1.1 mg/ml과 250 nmol/min이었다. N-말단의 아미노산 서열을 결정하여 본 결과, 식물이나 다른 진균류에서 보고된 아미노산 서열과 유사성이 없었다.

• 생명과학회지
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• 제19권9호
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• pp.1299-1303
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• 2009

뇌졸중 환 6명을 대상으로 발병후 3개월 지난 시기에 1차 실험을 실시하였고, 6개월 지난 시기에 2차 실험을 하여 뇌졸중환자들을 대상으로 운동학적변인, 각운동학적변인 그리고 지면반력과 발바닥의 압력분포를 알아보았다. 운동학적변인의 결과 통계적으로 유의한 차이는 나타나지 않았으나 6개월간 꾸준히 재활운동에 참가한 환자는 3개월 때 보다 훨씬 보폭(step length)이 크게 나타났으며(11.18 cm, 12.25 cm, p=0.58), 보폭의 빈도(step time) 또한 빠르게 나타났다(1.20 sec, 1.18 sec, p=0.53). 환측의 발만 지지면에 닿아 있는 시간(single support time)의 연구에서는 재활운동에 꾸준히 참가한 환자가 발을 지면으로부터 빨리 떼는 것으로 나타났다(34.97%, 29.02%, p=0.74). 환측다리 움직임의 빠르기를 나타내는 변인(cadence)에서는 1분당 움직임의 회수가 6개월 운동에 참가한 사람의 보폭회수가 3개월 운동에 참가한 사람의 보폭회수 보다 훨씬 빠르게 나타났다(21.04 step/min, 28.89 step/min, 0.27). 각 운동학변인에서는 통계적으로 모든 변인에서 유의한 차이는 나타나지 않았으나 평균의 비교에서 꾸준하게 6개월 동안 재활운동에 참가한 환자는 발목관절, 무릎관절 그리고 엉덩관절 모두의 가동범위가 크게 나타났다. 걷기동작에서 상해의 원인이되는 최초 접촉 순간의 충격력(impact force)의 비교에서는 6개월간 꾸준히 재활운동에 참가한 환자는 충격력이 작게 나타났다(1.20 N/kg, 1.13 N/kg, p=0.45). 전방으로의 추진력(anterior propulsion force)과 후방으로의 감속력(posterior braking force)은 통계적으로 유의한 차이(p=0.28, p=0.39)를 나타내지 않았으나 재활운동에 꾸준히 참가한 환자들은 전후방의 힘(0.10 N/kg, 0.15 N/kg; -0.12 N/kg, -0.18 N/kg)이 좋아지는 것을 볼 수 있었다. 걷기 동작 시 환측발의 최대 압력분포값은 통계적으로 유의한 차이(p=0.24)는 없었으나 6개월 재활훈련참가자(15.51 N/$cm^2$)가 3개월 재활훈련참가자(13.99 N/$cm^2$) 보다 지면을 더욱 가볍게 밟고 지나가는 것으로 나타났다. 위의 연구결과들을 바탕으로 볼 때 뇌졸중 환자들은 꾸준하게 재활운동에 참가하여야 하며 주위 사람들의 관심이 항상 필요하다고 사료된다.

• The Journal of Advanced Prosthodontics
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• 제3권4호
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• pp.216-220
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• 2011

PURPOSE. Heat of composite polymerization (HP) indicates setting efficacy and temperature increase of composite in clinical procedures. The purpose of this in vitro experimental study was to evaluate the effects of 5 temperatures on HP of two new composites. MATERIALS AND METHODS. From each material (Core Max II [CM] and King Dental [KD]), 5 groups of 5 specimens each were prepared and their total HPs (J/gr) were measured and recorded, at one of the constant temperatures $0^{\circ}C$, $15^{\circ}C$, $23^{\circ}C$, $37^{\circ}C$ and $60^{\circ}C$ ($2{\times}5{\times}5$ specimens) using a differential scanning calorimetry (DSC) analyzer. The data were analyzed using a two-way ANOVA, a Tukey's test, an independent-samples ttest, and a linear regression analysis (${\alpha}=0.05$). RESULTS. No polymerization reactions occurred at $0^{\circ}C$; then this temperature was excluded from statistical analyses. The mean HP of the remaining 20 KD specimens was $20.5{\pm}14.9$ J/gr, while it was $40.7{\pm}12.9$ J/gr for CM. The independent-samples t-test showed that there were significant differences between the HP of the two materials at the temperatures $15^{\circ}C$ (P=.0001), $23^{\circ}C$ (P=.0163), $37^{\circ}C$ (P=.0039), and $60^{\circ}C$ (P=.0106). Linear regression analysis showed statistically significant correlations between environment temperatures and HP of CM ($R^2=0.777$). CONCLUSION. Using CM is advantageous over conventional composite because of its better polymerization capacity. However due to its high HP, further studies should assess its temperature increase in vivo. Preheating KD is recommended. Refrigerating composites can negatively affect their polymerization potential.

• 천문학회지
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• 제53권1호
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• pp.9-26
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• 2020

At q = 1.81 ± 0.20 × 10^-5, KMT-2018-BLG-0029Lb has the lowest planet-host mass ratio q of any microlensing planet to date by more than a factor of two. Hence, it is the first planet that probes below the apparent "pile-up" at q = 5-10 ×10^-5. The event was observed by Spitzer, yielding a microlens-parallax π_E measurement. Combined with a measurement of the Einstein radius θ_E from finite-source effects during the caustic crossings, these measurements imply masses of the host M_host = 1.14^+0.10_-0.12 M_⊙ and planet M_planet = 7.59^+0.75_-0.69 M_⊕, system distance D_L = 3.38^+0.22_-0.26 kpc and projected separation a_⊥ = 4.27^+0.21_-0.23 AU. The blended light, which is substantially brighter than the microlensed source, is plausibly due to the lens and could be observed at high resolution immediately.