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Effect of Samsoeum on Cytokine Regulation of Mouse T cell (삼소음 알코올 추출물이 생쥐 면역세포의 시토킨 조절에 미치는 효과)

  • Ryu, Nae-Hyung;Park, Sung-Min;Kang, Hee;Shim, Bum-Sang;Kim, Sung-Hoon;Choi, Seung-Hoon;Ahn, Kyoo-Seok
    • Journal of Physiology & Pathology in Korean Medicine
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    • v.22 no.4
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    • pp.856-862
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    • 2008
  • The purpose of this study was to evaluate the effect of Samsoeum(SSE) on cytokine regulation of mouse T cells. The proliferation of mouse CD4 T cells under the influence of SSE extract was measured. When mouse CD4 T cell were stimulated with anti-CD3 and anti-CD28 in various concentrations of SSE extract, it increased proliferation of CD4 cells by 30% in $50{\mu}g/m{\ell}$ concentration. The proliferation of CD4 cells increased in $100{\mu}g/m{\ell}$ and $200{\mu}g/m{\ell}$. Treatment of CD4+ T cells stimulated by anti-CD3e and anti-CD28 with SSE resulted in reduction of $IFN-{\gamma}$ and IL-4 levels. SSE has dose-dependent inhibitory effect on $IFN-{\gamma}$ and decreased IL-4 by 70% at 50, $200{\mu}g/m{\ell}$. Oral administration of SSE resulted in increase of CD8+ T cell population in Balb/c mice by 8%. CD4+ T cells under Th1/Th2 polarizing conditions for 3 days with SSE resulted in decrease of $IFN-{\gamma}$ level in Th1 cells by 44% and increase of IL-4 level in Th2 cells by 60%. Experimental results of this study show that SSE induces mouse T-cell to transform into Th2, and increases T-cell population and activation.

Effects of weir on the change of sediments (하천의 보가 퇴적물에 미치는 영향)

  • Cho, Yong-Chul;Kim, Kyung-Hee;Woo, Hyo-Seop;Oh, Jong-Min
    • Proceedings of the Korea Water Resources Association Conference
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    • 2006.05a
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    • pp.1083-1087
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    • 2006
  • 과거 50 년 동안 국내 보 설치 수는 매년 증가하여 왔으나, 그 증가비율은 근래 들어 감소하는 추세로, 이는 보 보다는 용수공급능력이 큰 양수장, 저수지 등 타 시설물에 의존하는 것으로 판단 할 수 있다. 더욱이 인구의 도시 집중화에 따른 농경지의 도시화, 경작 방식의 변화에 의한 비닐하우스 단지로의 변화, 시설의 노후화 등으로 매년 $50{\sim}150$개 정도의 보가 폐기되고 있다. 그러나 이렇게 폐기되는 보는 하천에 그대로 존치되어 하천생태통로의 단절, 수질악화, 수변 서식처 악화 등의 문제를 지속적으로 야기 시키고 있다. 실험은 문산천 검전보, 안양천 안양대교보, 기철거된 경안천 무명보에서 자연시료를 채취하여 실행 하였다. 문산천 검전보는 하천환경이 안정되고 양호한 대조구간으로 선정하였다. 안양 대교보는 도심화로 인하여 원기능을 상실하였으며 수차례에 걸친 개수가 이루어졌고 수년전까지만 해도 러브보로 인한 수질정화장치가 있었으나 효능이 약화되어 철거할 계획을 가지고 있는 것으로 알려져 있다. 경안천 무명보는 현재는 완전 철거된 상태이다. 경안천 무명보는 기 철거된 보의 대조구간으 로 선정하였다. 보 상류의 수질 오염도가 높은 것은 정체구역에 의한 용존산소 부족과 오염저니에 의한 것으로 판단되므로, 각각의 대상보의 퇴적물 오염도를 조사하였다. 각각의 조사 지점은 검전보의 경우, 만장교를 최상류 지점으로, 검전보 상/하류, 광탄교를 최하류 지점으로 선정하여, 총 4 지점에서 채취했으며, 안양대교보의 경우 최상류지점, 보 상/하류 지점으로 총 3지점, 무명보의 경우 보 상/하류로 총 2 지점에서 채취했으며, 실험은 $\ulcorner$Soil Method$\lrcorner$에 준하여, 각각 입도분포, 함수율, 강열감량, COD, T-N, T-P 등을 시행하였다. 문산천 검전보 퇴적물 오염도 조사 결과, 강열감량, COD, T-N, T-P 항목에서 보 상류가 보 하류에 비해 높게 나타났으며, 각각 하류에 비해 상류가 30.76%, 16.69%, 73.13%, 18.91% 더 높게 나타났다. 안양대교보 퇴적물 조사 결과, 검전보와 마찬가지로, 강열감량, COD, T-N, T-P 항목에서 보 상류가 보 하류에 비해 33.33%, 9.23%, 24.78%, 18.56% 높게 나타났다. 기 철거된 무명보의 경우, 무명보 상류, 무명보 하류 2개 지점을 채취하였으며, 강열감량, COD, T-N 항목에서 보 상류가 보 하류에 비해 18.75%, 412.6%, 25.75% 높게 나타났다. 퇴적물 조사 결과, 강열감량, COD, T-N 항목에서 검전보, 안양대교보, 무명보 모두 보 상류의 오염도가 높게 나타났으며, 무명보를 제외한 검전보, 안양대교보는 T-P 항목에서도 보 상류의 오염도가 높게 나타나, 보 상류의 퇴적물 오염농도가 심화된 것으로 나타났다. 그러나 기철거된 무명보의 경우, 강열감량, COD, T-N, 항목에서 보 상류가 높게 나타났으나, 철거되지 않은 검전보나 안양대교보에 비해 그 차이가 크지 않은 것으로 나타났다.

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Effect of Cattle Manure Application on Forage Productivity and Soil Characteristics of Pasture (방목초지에서 우분 퇴비 시용이 목초생산 및 토양에 미치는 영향)

  • Hwang, Kyung-Jun;Ko, Seo-Bong;Park, Hyung-Soo;Park, Nam-Gun;Ko, Moon-Suck;Kim, Moon-Chul;Song, Sang-Teak
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.27 no.1
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    • pp.45-52
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    • 2007
  • This study was conducted from 2003 to 2005 at crop field of National Institute of Subtropical Agriculture, RDA, in Jeju. The treatment consisted of chemical fertilizer 100%(CF 100%, T1), cattle manure 100%(CM 100%, T2), CF 50%+CM 50%(T3), CF 25%+CM 75%(T4), CM 100%(1st yr.)+CF 100%(2nd yr,)+CM(3rd yr)(T5), CM 100%(1st yr.)+CF 100%(2nd yr.)+CF(3rd yr.)(T6). The highest substitution effect of chemical fertilizer for cattle manure indicated that the highest yield(Avg. 11,169 kg/ha) obtained from CM 100%(1 yr.)+CF 100%(2 yr.) treatment. The forage yield of CM 100%(1 yr.)+CF 100%(2 yr.) was 4% more than CM 100% application whereas was 3% less than CF 100% application. Changes of physical and chemical properties of soils for applications of CF 100% and CM 100% was clearly in cattle manure application. Especially, cattle manure application in pasture were superior to chemical fertilizer application with respect to organic matter(OM, %), total nitrogen(T-N, %), and avaliable phosphorous($P_2O_5$, %) contents of soils. In this present study, cattle manure application recorded slightly lower forage yields than CF 100% application but the combined use of cattle manure and chemical fertilizer was attribute to reduction of chemical fertilizer application rate as well as maintaining soil health on long-term basis.

The Effects of Team-Based Learning on Fundamentals of Nursing (기본간호학 수업에서 팀기반 학습 적용 효과)

  • Kim, Soon-Ok;Kim, Mi-sook
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.17 no.12
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    • pp.107-119
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    • 2016
  • This research was explored the effects of the application of the team-based classes on the self-directed learning, the academic self-efficacy, and the level of learning satisfaction of the nursing students. Study subjects are 104 nursing students from one university from G region. The data were analyzed by t-test, one-way ANOVA, paired t-test, Pearson's correlation coefficients. With regard to the differences between the levels of the learning satisfaction, the differences were shown (t=4.410, p<.05). Regarding the self-directed learning, it rose to the average of 3.50 points after the application of the team-based learning from the average of 3.39 points. It showed a difference (t=-2.083, p=<.05). And the academic self-efficacy rose to 3.65 points from the 3.12 points after the application of the self-based learning. And it showed a difference (t=-14.175, p=<.001). The level of the learning satisfaction was shown to be over the middle with the average of 3.73 points. And the self-directed learning after the application of the team-based learning showed an academic self-efficacy (r=.512, p<.001), a level of the learning satisfaction (r=.421, p<.001), and a positive relationship. It was proven that the team-based learning can cultivate the capabilities for effectively solving the clinical problems.

Characterization of Trichosporonoides madida and Evaluation of Virulence in Laboratory Animals (Trichosporonoides madida 균주의 성상 및 실험동물에 대한 병원성)

  • Jong-Bae Kim;Young-Mi Kim;Sang-Wook Park
    • Biomedical Science Letters
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    • v.3 no.2
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    • pp.77-82
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    • 1997
  • One of the most important prerequisites of the industrial microorganism is that it should not be virulent to humans or economically important animals or plants. In this investigation, the microbiological characterization of T. madida N-5-3 strain was performed. And then, the virulence of the test strain in mouse model was examined systematically. The microbiological characteristics of the test strain were found to be fully consistent with those of typical T. madida. The i.p. lethal dose(LD)$_{50}$ of the test strain was greater than 1$\times$10$^8$, because there was no dead animal with the challenge doses upto the level of 1$\times$10$^8$. When 1$\times$10$^8$ yeast cells were challenged to the laboratory mice, T. madida N-5-3 strain was completely cleared from the liver and spleen in 4 days after challenge. And no pathological changes in the histological examination of the internal organs from challenged mice was observed. Above results can provide the predictability of the safety of T. madida N-5-3 strain for the industrial use in the view point of the public health aspect.

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A Study on the Relationship of Infection Control Performance in a Long Term Care Hospital Caregivers (일개 지역 요양병원 간병인의 감염관리수행도 관련성 연구)

  • Hong, Na-Kyung;Kang, Kyung-Ja
    • Journal of Digital Convergence
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    • v.18 no.1
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    • pp.187-198
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    • 2020
  • The purpose of the study is to investigate infection management status of long term care hospitals and infection prevention knowledge of caregivers. This is descriptive study to investigate the factors affecting caregivers' infection control performance in long term care hospitals. The data collection period was from August, 2018, and a total of 197 caregivers from 8 long term care hospitals. The data analysis were done Win SPSS 20.0 with t-test, ANOVA, pearson's correlation coefficient, and hierarchical multiple regression analysis. Infection prevention knowledge was positively correlated with infection control performance (p=<.001). The factors influencing performance in a long term care hospital caregivers were over seventy years old (t=2.50, p=.013), eight-hour working time (t=-2.62, p=.010), nursing staffing First grades (t=2.48, p=.014), and infection prevention knowledge (t=2.96 p=.003), which explained 12.9% (F=6.70 p<.001). In summary, we recommend to develop infection education interventions to improve caregivers' infection control performance in long term care hospitals and to improve of the function in long term care hospitals.

Study on Optical Characteristics of Organic Light-emitting Diodes Using Two Fluorescence Dopants in Single Emissive Layer (2개의 형광 도판트를 적용한 단일발광층 유기발광소자의 광학적 특성 연구)

  • Kim, Tae-Gu;Oh, Hwan-Sool;Kim, You-Hyun;Kim, Woo-Young
    • Journal of the Korean Vacuum Society
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    • v.19 no.3
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    • pp.184-189
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    • 2010
  • Organic light-emitting diodes (OLEDs) with single emissive layer structures using two fluorescent dopants were fabricated and the device was composed of ITO / NPB ($700{\AA}$) / MADN : C545T - 1.0% : DCJTB - 0.3% ($300{\AA}$) / Bphen ($300{\AA}$) / LiF ($10{\AA}$) /Al ($1,000{\AA}$). C545T and DCJTB were functioned as green fluorescent dye and red fluorescent dye under MADN as host material. Concentrations of C545T and DCJTB was changed in emissive layer of MADN. Optimized OLED device using two fluorescence dopants shows emission efficiency of 8.42 cd/A and luminescence of 3169 cd/$m^2$at 6 V with CIE color coordinate, (0.43, 0.50). Electroluminescence of optimized OLED showed two peak at 500 and 564 nm according to C545T and DCJTB. These results indicate that F$\ddot{o}$ster energy transfer energy transfer was from MADN to C545T and rather than to DCJTB continuously.

Isolation and Degradation Activity of a TBTCl (Tributyltin Chloride) Resistant Bacteriain Gwangyang Bay (광양만에서 TBTCl (Tributyltin Chloride) 내성세균의 분리 및 분해활성)

  • Jeong, Seong-Yun;Son, Hong-Joo;Jeoung, Nam-Ho
    • Korean Journal of Environmental Agriculture
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    • v.30 no.4
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    • pp.424-431
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    • 2011
  • BACKGROUND: Tributyltin chloride is among the most toxic compounds known for aquatic ecosystems. Microorganisms are responsible for removal of TBTCl. Nevertheless, only a limited number of marine bacteria were investigated for biodegradation of TBTCl in Korea. METHODS AND RESULTS: The number of TBTCl resistant bacteria ranged from $2.5{\times}10^3$ to $3.8{\times}10^3$ cfu/mL in the seawater, and ranged from $3.2{\times}10^5$ to $9.1{\times}10^5$ cfu/g in the surface sediment, respectively. The morphological, physiological, and biochemical characteristics of TBTCl resistant bacteria were investigated by API 20NE and other tests. The most abundant species of TBTCl resistant bacteria were Vibrio spp. (19.2%), Bacillus spp. (16.2%), Aeromonas spp. (15.2%), and Pseudomonas spp. (13.1%), etc. Eleven TBTCl resistant isolates also had a resistance to heavy metals (Cd, Cu, Hg, and Zn). Among them, isolate T7 showing the strong TBTCl-resistance was selected. This isolate was identified as the genus Pantoea by 16S rRNA gene sequencing and designated as Pantoea sp. T7. In addition, this bacterium was cultivated up to the growth of 50.7% after 60 hrs at TBTCl concentration of $500{\mu}M$. TBTCl-degrading activity of Pantoea sp. T7 was measured by GC-FPD analysis. As a result of biological TBTCl-degradation at TBTCl concentration of $100{\mu}M$, TBTCl-removal efficiency of Pantoeasp. T7 was 62.7% after 40 hrs. CONCLUSION(S): These results suggest that Pantoea sp. T7 is potentially useful for the bioremediation of TBT contamination.

The effect of Plantaginis asiaticae Folium on lipid accumulation in 3T3-L1 adipocytes by extraction conditions (추출조건에 따른 차전초 추출물이 3T3-L1 세포의 지방 축적에 미치는 영향)

  • Jeong, Mi-Jin;Kim, So-Young;Kim, Yoo-Jin;Lee, Eun-Tag;Choo, Sung-Tae;Kim, Han-Hyuk;Kim, Mi Ryeo
    • The Korea Journal of Herbology
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    • v.33 no.2
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    • pp.53-58
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    • 2018
  • Objectives : Plantaginis asiaticae Folium (PA) has been widely used in Korean medicine for treatment of liver disease, stomach troubles and inflammation. We investigated the effect of PA on lipid accumulation in 3T3-L1 according to extraction conditions. Methods : The MTT assay was employed to evaluate the cytotoxicity of PA extracted by different solvents (water, 50% ethanol, and 95% ethanol) on 3T3-L1 preadipocytes. Oil red O staining was used to identify intracellular lipid accumulation in 3T3-L1. 3T3-L1 adipocytes were treated with PA at concentration ranging in 0.1, 0.2 and $0.4mg/m{\ell}$. PA was extracted by different extraction conditions such as extraction solvents, extraction time, and extraction temperature. In addition, UPLC analysis was used for determination of candidates of active ingredients in PA. Results : 3T3-L1 preadipocytes were treated with PA extracted by different solvents (water, 50% ethanol, and 95% ethanol) and there was no cytotoxicity. Oil red O staining was employed to identify the effect of PA on lipid accumulation in 3T3-L1. In the present study, PA water extraction at $70^{\circ}C$ for 6 hours decreased greatly in lipid accumulation. The range of concentrations was 0.1, 0.2 and $0.4mg/m{\ell}$. Concentration at $0.2mg/m{\ell}$ was the most effective one among them. Candidates of active ingredients in PA were shown plantamajoside and acteoside through UPLC. Conclusions : These results suggest that the effect of PA water extraction at $70^{\circ}C$ on lipid accumulation in 3T3-L1 is superior to other extraction conditions. We suppose that plantamajoside and acteoside may be candidates of active ingredients in PA.

Effect of Cryoprotectants on Quality Properties of Chicken Breast Surimi Manufactured by pH Adjustment (냉동변성방지제 조건에 따른 닭가슴살 수리미의 저장특성)

  • Jin, S.K.;Kim, I.S.;Choi, Y.J.;Park, G.B.;Yang, H.S.;Kim, B.G.
    • Journal of Animal Science and Technology
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    • v.50 no.1
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    • pp.99-110
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    • 2008
  • This study was conducted to determine the effect of pH adjustment and the addition of cryoprotectants on the storage properties of chicken breast surimi. Surimi as a control was prepared with Alaska pollack by two times of washing treatment and addition of cryoprotectants(4% sugar, 5% sorbitol and 0.3% polyphosphate). Three types of surimi were manufactured by different cryoprotectant conditions(T1: 5% sorbitol+0.3% polyphosphate, T2: 4% sugar+5% sorbitol+0.3% polyphosphate, T3: 2% salt+4% sugar+5% sorbitol+0.3% polyphosphate) and frozen after adjusting the surimi to pH 11.0. The amino acid and saturated fatty acid composition were significantly higher in the control. EAA(essential amino acid), FAA(amino acid in relation to flavor), SAAA(amino acid in relation to saccarinity) and FRAA (fragrant amino acid) were significantly higher in the control. The TBARS(thiobarbituric acid reactive substances) and VBN(volatile basic nitrogen) were increased with storage times. TBARS and VBN values were significantly higher in the control and T3 than the other treatments. The VBN was significantly higher in control and T3 than other surimi samples. Chicken breast surimi adjusted to pH 11.0 had higher in microorganisms than the control, and T1 sample had the highest total plate count. Sensory evaluations were significantly higher in the control and T3 than the other samples. Especially, the overall acceptability of T3 was similar to the control.