The Korea Journal of Herbology (대한본초학회지)

The Korea Association of Herbology (대한본초학회)
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The effect of Plantaginis asiaticae Folium on lipid accumulation in 3T3-L1 adipocytes by extraction conditions

추출조건에 따른 차전초 추출물이 3T3-L1 세포의 지방 축적에 미치는 영향

  • Jeong, Mi-Jin (Department of Herbal Pharmacology, Daegu Hanny University) ;
  • Kim, So-Young (Department of Herbal Pharmacology, Daegu Hanny University) ;
  • Kim, Yoo-Jin (Department of Herbal Pharmacology, Daegu Hanny University) ;
  • Lee, Eun-Tag (Ebiche co. Ltd.) ;
  • Choo, Sung-Tae (Ebiche co. Ltd.) ;
  • Kim, Han-Hyuk (Medical Convergence Textile Center Reaserch & Certification Team, Gyeongbuk Technopark Foundation) ;
  • Kim, Mi Ryeo (Department of Herbal Pharmacology, Daegu Hanny University)
  • 정미진 (대구한의대학교 한의과대학 본초약리학교실) ;
  • 김소영 (대구한의대학교 한의과대학 본초약리학교실) ;
  • 김유진 (대구한의대학교 한의과대학 본초약리학교실) ;
  • 이은탁 (농업회사법인(주) 이비채) ;
  • 추성태 (농업회사법인(주) 이비채) ;
  • 김한혁 (경북테크노파크 첨단메디컬융합섬유센터) ;
  • 김미려 (대구한의대학교 한의과대학 본초약리학교실)
  • Received : 2018.02.10
  • Accepted : 2018.03.15
  • Published : 2018.03.30
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Abstract

Objectives : Plantaginis asiaticae Folium (PA) has been widely used in Korean medicine for treatment of liver disease, stomach troubles and inflammation. We investigated the effect of PA on lipid accumulation in 3T3-L1 according to extraction conditions. Methods : The MTT assay was employed to evaluate the cytotoxicity of PA extracted by different solvents (water, 50% ethanol, and 95% ethanol) on 3T3-L1 preadipocytes. Oil red O staining was used to identify intracellular lipid accumulation in 3T3-L1. 3T3-L1 adipocytes were treated with PA at concentration ranging in 0.1, 0.2 and $0.4mg/m{\ell}$. PA was extracted by different extraction conditions such as extraction solvents, extraction time, and extraction temperature. In addition, UPLC analysis was used for determination of candidates of active ingredients in PA. Results : 3T3-L1 preadipocytes were treated with PA extracted by different solvents (water, 50% ethanol, and 95% ethanol) and there was no cytotoxicity. Oil red O staining was employed to identify the effect of PA on lipid accumulation in 3T3-L1. In the present study, PA water extraction at $70^{\circ}C$ for 6 hours decreased greatly in lipid accumulation. The range of concentrations was 0.1, 0.2 and $0.4mg/m{\ell}$. Concentration at $0.2mg/m{\ell}$ was the most effective one among them. Candidates of active ingredients in PA were shown plantamajoside and acteoside through UPLC. Conclusions : These results suggest that the effect of PA water extraction at $70^{\circ}C$ on lipid accumulation in 3T3-L1 is superior to other extraction conditions. We suppose that plantamajoside and acteoside may be candidates of active ingredients in PA.

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References

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