• The Korean Journal of Soil Zoology
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• v.5 no.2
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• pp.85-96
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• 2000

Four new species of predatory nematodes belonging to the Actinolaimoidea, Dorylaimida are described. Neoactinolaimus gosungensis n. sp. is 2.2-2.4 mm long, b = 4.3-4.9; c = 10.7-11.2; species 60-62 ${\mu}{\textrm}{m}$ long and is distinguishable by low lip region, asymmetrical odontostyle and its wider lumen. Egtitus arcuatus n. sp. is 1.5-1.8 mm long, b = 3.6-4.2; c = 14-15 and is characterized by having dorsally arcuate odontostyle and presence of cardiac glands at base of oesophagus. Paractinolaimus tuberculantus n. sp. is 1.9-2.5 mm long, b = 3.3-4.0, c = 7.2-8.8, spicules 66-69 ${\mu}{\textrm}{m}$ long and is unique in having coiled body posture and a tubercle at the base of oesophagus. Carcharolaimus koriensis n. sp. is 1.7-1.9 mm long, b = 3.6-4.5, c = 65-76 and is characterized by having very thick body cuticle and larger odontostyle aperture.

• Applied Biological Chemistry
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• v.40 no.5
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• pp.369-374
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• 1997

An endochitosanase-producing bacterium was isolated from soil and identified as a strain of Bacillus sp. The isolate was gram positive, rod shape $(0.4-0.6{\times}1.6-2.2{\mu}m)$, endospore-forming, catalase positive, and mobility positive, and grown at pH 4.5-11.0 and upto $42^{\circ}C$ in the medium containing 2% NaCl. RAPD analysis of the DNA purified from the strain was also performed, and the chitosanase-producing strain was named as Bacillus sp. P16. The culture supernatant of the strain showed strong liquefaction activity and rapidly decreased viscosity of chitosan solution. By TLC and HPLC, chitooligosaccharides of DP 2-7 were separated and identified from the enzyme hydrolyzates of chitosan. The chitosanase from Bacillus sp. P16 was thus regarded as an endo-splitting type.

• Korean Journal of Soil Science and Fertilizer
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• v.40 no.6
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• pp.442-446
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• 2007

From the rhizoplane and rhizosphere of pepper, tomato, lettuce, pasture, and grass, unsoluble inorganic phosphate solubilizing bacterial strains were isolated using plate base assay on Pikovskaya's medium. Two strains, CL-1 and CL-2, which produced largest halo on plates (indicative of phosphate solubilization)were selected for further studies. Based on these biochemical and 16S rRNA analysis strains CL-1, CL-2 were found to be as species of Pseudomonas sp. and Kluyvera sp., respectively. In broth assay Pseudomonas sp. CL-1 and Kluyvera sp. CL-2 solubilized insoluble phosphate by 193.4 mg and $493.6P\;mg\;L^{-1}$, respectively after $3^{rd}$ day inoculation. These effecient phosphate solubilizing bacteria have a potential to be developed as microbial based fertilizer in future.

• Journal of Life Science
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• v.22 no.2
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• pp.167-176
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• 2012

Three protease-producing halophilic bacteria were isolated from fermenting anchovy. Isolated FAM 10, FAM 114, and FAM 115 were found to grow optimally at salt concentrations of 2-4%, 10%, and 6%, respectively, and could grow in salinity of up to 18-22%. The salinity conditions for optimum protease production were 6% in FAM 10 and 10% in FAM 114 and FAM 115. The protease activity of FAM 10 was gradually inhibited by the addition of NaCl up to 10%, and was not evident at 14%, whereas FAM 114 and FAM 115 displayed protease activity at 14% NaCl and could not be measured at 18%. These results demonstrated that the three isolated strains belong to protease-producing, moderately halophilic bacteria. Strain FAM 10, FAM 114, and FAM 115 were identified as Salinivibrio sp., Halobacillus sp., and Halobacillus sp. respectively, based on comparative analyses of the 16S rRNA gene and the 16S-23S intergenic space sequence (IGS), biochemical testing, and Gram staining. Salinivibrio sp. FAM 10 had two 16S rDNAs containing different sequences at position 191 and four IGSs that harbored no tRNA gene and tRNA genes for isoleucine, alanine, glutamate, lysine, and/or valine. Halobacillus sp. FAM 114 and FAM 115 had completely identical 16S rRNA gene sequences and showed 99% identity to the sequences of various Halobacillus strains. The three IGSs found in the genome of both strains displayed 99% sequence identity with Halobacillus aidingensis and Halobacillus sp. JM-Hb, and had $IGS^0$ with no tRNA gene and $IGS^{IA}$ with tRNA genes for isoleucine and alanine.

• Journal of Life Science
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• v.29 no.2
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• pp.158-163
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• 2019

The purpose of this study was to isolate a new marine agarase-producing bacterium. Agarase can hydrolyze agar and agarose to produce agarooligosaccharides or neoagarooligosaccharides, which possess many physiological functions. Strain DH-3 was isolated from seawater collected from the coast of Yeosu at Jeollanam province, Korea. A 16S rDNA sequence analysis showed this strain to be Persicobacter sp. DH-3. Extracellular agarase was prepared from culture media of Persicobacter sp. DH-3 and used for characterization. Relative activities at 20, 30, 40, 50, 60, and $70^{\circ}C$ were 50, 55, 70, 100, 90, and 50%, respectively. Relative activities at pH 5, 6, 7, and 8 were 75, 100, 90, and 75%, respectively. The enzyme showed maximum activity at $50^{\circ}C$ in a 20 mM Tris-HCl buffer at pH 6. This enzyme could be useful, as agar is in liquid state at $50^{\circ}C$. Agarase activities were maintained at 80% or more for 2 hr at 20, 30, and $40^{\circ}C$. Thin layer chromatography analysis suggested that Persicobacter sp. DH-3 produced extracellular ${\beta}$-agarases as it hydrolyzed agarose to produce neoagarohexaose and neoagarotetraose. In addition, zymogram analysis confirmed that Persicobacter sp. DH-3 produces at least three agar-degrading enzymes with molecular weights of 45, 70, and 140 kDa. Therefore, it is expected that agarases from Persicobacter sp. DH-3 could be used to produce functional neoagarooligosaccharides.

• Journal of Nutrition and Health
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• v.55 no.1
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• pp.59-69
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• 2022

Purpose: Natural medicinal plant extracts have recently attracted attention as health beneficial foods and potential therapeutic agents for prevention of various diseases. This study was undertaken to measure the anti-inflammatory effect of the ethanol-water fraction obtained from the above-ground portion of Spiraea prunifolia var. simpliciflora, a wild-growing plant in Korea. The final fraction used in this study was the H₂O-EtOH (40:60) fraction (SP60), which had the highest antioxidant activity, as determined in previous studies. Methods: The amounts of nitric oxide (NO), tumor necrosis factor (TNF)-α, interleukin (IL)-6 and IL-1β production were measured in lipopolysaccharide (LPS)-stimulated RAW 264.7 cells exposed to SP60. Western blot was performed to measure the expressions of inducible nitric oxide synthase (iNOS), cyclooxygenase (COX)-2, and the activation of nuclear factor (NF)-κB. Results: SP60 exerted no cytotoxicity up to concentrations of 125 ㎍/mL. The levels of inflammatory cytokines, such as NO, TNF-α, IL-6, and IL-1β, were significantly decreased in LPS-stimulated RAW264.7 cells exposed to SP60. In addition, the expression levels of iNOS, COX-2, and phosphorylated p65 showed a concentration-dependent decrease subsequent to SP60 treatment. These results indicate that SP60 inhibits the LPS-induced production of inflammatory cytokines, iNOS, and COX-2, by inhibiting the activation of NF-κB, which is responsible for the expression of inflammatory mediators. Conclusion: The results presented in this study indicate that the H₂O-EtOH (40:60) fraction (SP60) extracted from the above-ground portion of Spiraea prunifolia var. simpliciflora has the potential to be developed as a medicine or healthcare food and functional material possessing anti-inflammatory effects. However, it is necessary to first confirm the anti-inflammatory effects of SP60 in in vivo models.

• The Korean Journal of Mycology
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• v.9 no.4
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• pp.193-197
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• 1981

Nematode destroying-fungi from ginseng field were isolated and then identified on 2% wateragar and malt extract agar media. 1. Six strains of Arthrobotrys sp. and three strains of Harposporium sp. were isolated and identified. 2. Arthrobotrys sp. formed trapping apparatus when they were cultured with Nematode and appeared to be destroying fungi. 3. Harposporium sp. appeared to be endoparasitic fungi. 4. Both Arthrobotrys sp. and Harposporium sp. were grown well on nutrient agar and malt extract agar.

• Microbiology and Biotechnology Letters
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• v.30 no.3
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• pp.253-257
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• 2002

The time-dependent changes of red pigments production in solid-state plant scale fermentor using barley cultured with Monascus sp., instead of rice which was traditionally used, were investigated in this study. A steady increase in the yield of red pigments in barley occurred between the 3rd and 6th days. The optimized conditions (inoculation volume = 6∼8%, initial pH = 6, air supply = 0.6∼0.8 m) promoted the production of red pigments. Short-time steaming of barley (< 20 min) decreased fungal growth and pigments production due to the insufficient gelatiniza-tion. The optical density of the red pigments under the optimized conditions was 120 at 500 ]nm per gram of barley. In addition, the metabolites from the fermented barley with Monucus sp. showed antibacterial effects against Escherichia coli and Salmonella typhimurium. Barley was shown to be one of the best grain sources for solid-state fermentation with Monascu sp., fur obtaining natural pigments and also functional food materials.

• Korean Journal of Environmental Agriculture
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• v.25 no.2
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• pp.124-128
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• 2006

To reducing offensive odor form compost UIW-6 mutant obtained by UV treatment from sulfur-oxidizing bacteria, Thiobacillus sp. IW. The UIW-6 mutant was found 1.6 times faster in growth than the parent strain on thiosulfate medium (TM) at 36 h after incubation. Initial pH, temperature and agitation for the optimum growth of UIW-6 were 6.5, $35^{\circ}C$ and 200 rpm, respectively. The UIW-6 mutant growth was two times higher than parent strain at 6 h culture in TM liquid medium containing 50 mM sodium thiosulfate. The UIW-6 mutant used fructose and sucrose as carbon sources and yeast extract> tryptone> peptone as nitrogen ones. It was found that the growth of UIW-6 was increased in addition of 0.2% yeast extract.

• Korean journal of applied entomology
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• v.35 no.4
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• pp.273-279
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• 1996

Based on the material in the Center for Insect Systematics, Chuncheon, Korea and Institute of Biology and Pedology, Vladivostok, Russia, 4 species of tineids are described as new to science: Morophaga parabucepkala sp. nov., Ceratutzcus orientatzus sp. nov., Gerontha ampliptera sp. nov., and G. namhaensis sp. nov. Furthermore 6 species are recognized new to Korea: , and 4 species new to Russian Far East: