• 대한상한금궤의학회지
• /
• v.4 no.1
• /
• pp.75-82
• /
• 2012

Objective : We considered Astragali Radix(黃耆) can be used to cure serious atopic dermatitis. so we treated this patient with prescriptions contained Astragali Radix(黃耆). By this case, we got effective results and report this case and index of choice Astragali Radix(黃耆) Method : After treatment with prescription contained Astragali Radix(黃耆), we observe the changes and analyze results. Results : 1. Atopic dermatitis can be cured with Astragali Radix's efficiency on Sanghanron(傷寒論) 2. We considered Astragali Radix(黃耆) can be used when Chronic damage to the nasal mucosa is appeared by rhinoscopy and Sneezing, runny nose that lasts all year round is shown Conclusion : Gaeji-Ka-Hwangki-Tang(桂枝加黃耆湯) has most effect on this case. So, We need to study combination Astragali Radix(黃耆) with Glycyrrhizae Radix(甘草) and the efficacy of herbs belonged to leguminosae.

• The Korea Journal of Herbology
• /
• v.31 no.1
• /
• pp.77-82
• /
• 2016

Objectives : The study was designed to observe the effect of Polygalae Radix Preparata Cum Glycyrrhizae Radix on 4-Hydroxynonenal (4-HNE)-induced apoptosis in PC-12 cell.Methods : A cytotoxic test on Polygalae Radix Preparata Cum Glycyrrhizae Radix (PG) was conducted and another MTT assay was conducted to observe the cytoprotective effect against 4-HNE that cause oxidative stress. In addition, in order to observe the expression of Bax, Bcl-2, Caspase-3 and TNF-α protein involved with apoptosis, western blot was conducted.Results : The groups treated with 25 ㎍, 50 ㎍ and 100 ㎍ of PG water extract had no toxicity for PC-12 cell. The groups treated with 25 ㎍, 50 ㎍ and 100 ㎍ of PG water extract showed a significant increase of cell survival rate in comparison with the control group injected by only 4-HNE. The groups treated with 25 ㎍ and 50 ㎍ of PG water extract showed a significant supression on increase of Bax protein expression in the control group. The group treated with 100 ㎍ of PG water extract showed a significant promotion on decrease of Bcl-2 protein expression in the control group. The group treated with 50 ㎍ of PG water extract showed a significant supression on increase of Caspase-3 protein expression in the control group. The group treated with 25 ㎍ of PG water extract showed a significant supression on increase of TNF-α protein expression in the control group.Conclusions : These results suggest that Polygalae Radix Preparata Cum Glycyrrhizae Radix is effective in reducing apoptosis by 4-HNE-dameged cell.

• The Korea Journal of Herbology
• /
• v.30 no.1
• /
• pp.59-65
• /
• 2015

Objectives : The purpose of this study was to observe the effects of Polygalae Radix(PR) on 4-HNE-induced apoptosis in PC-12 cell. Methods : A MTT assay was conducted to observe the cytotoxicity of Polygalae Radix on the cell viability and the cytoprotective effect of Polygalae Radix against 4-HNE that causes oxidative stress-induced cytotoxicity, and then a western blot was conducted to observe the expression of $TNF-{\alpha}$, caspase-3, Bax and Bcl-2 protein that are important factors involved with apoptosis signaling pathway. Results : The Polygalae Radix water extract $25{\mu}g$, $50{\mu}g$, $100{\mu}g$ and $200{\mu}g/mL$ had no cytotoxicity on the PC-12 cell. The Polygalae Radix water extract $25{\mu}g$, $50{\mu}g$ and $100{\mu}g/mL$ had the cytoprotective effect against 4-HNE that causes cytotoxicity on the PC-12 cell. The Polygalae Radix water extract $50{\mu}g/mL$ significantly suppressed the increase in $TNF-{\alpha}$ protein expression in PC-12 cell. The Polygalae Radix water extract $25{\mu}g$ and $50{\mu}g/mL$ significantly suppressed the increase in caspase-3 protein expression in PC-12 cell. The Polygalae Radix water extract $25{\mu}g$, $50{\mu}g$ and $100{\mu}g/mL$ suppressed the increase in Bax protein expression in PC-12 cell but had no significance. The Polygalae Radix water extract $25{\mu}g$ and $100{\mu}g/mL$ significantly prevented the decrease in Bcl-2 protein expression in PC-12 cell, Conclusions : These results suggest that the Polygalae Radix water extract is effective in inhibiting apoptosis.

• Korean Journal of Oriental Medicine
• /
• v.16 no.1
• /
• pp.173-178
• /
• 2010

The purpose of this study was investigation of quantitative analysis of marker substances in solid fermented Angelicae Gigantis Radix by High performance liquid chromatography(HPLC). HPLC was performed for determination of nodakenin and decursin in solid fermented Angelicae Gigantis Radix extract, the separation method was performed on C18 column ($250\;mm\;{\times}\;4.6\;mm$, $5\;{\mu}m$, RS tech) using gradient solvent mixtures of water-acetonitrile with photodiode array detector (330 nm). The flow rate was 1.0 ml/min. Retention time of nodakenin and decursin was about 11.47, 46.79 min and linearity of calibration was showed good result(r2=0.9999, 0.9999), respectively. Content of nodakenin was $0.76\;{\pm}\;0.02%$ in control, $0.31\;{\pm}\;0.00%$ in Angelicae Gigantis Radix extract fermented with Paecilomyces japonica(SDT)(p<0.01), $0.51\;{\pm}\;0.02%$ in Angelicae Gigantis Radix extract fermented with Ganoderma lucidum(SYT)(p<0.01), $0.82\;{\pm}\;0.03%$ in Angelicae Gigantis Radix extract fermented with honey(SST)(p<0.05) and $0.88\;{\pm}\;0.01%$ in Angelicae Gigantis Radix extract fermented with Nuruk(SNT)(p<0.01). Content of decursin was $4.50\;{\pm}\;0.08%$ in control, $2.90\;{\pm}\;0.05%$ in Angelicae Gigantis Radix extract fermented with Paecilomyces japonica(SDT)(p<0.01), $2.65\;{\pm}\;0.08%$ in Angelicae Gigantis Radix extract fermented with Ganoderma lucidum(SYT)(p<0.01), $4.46\;{\pm}\;0.11%$ in Angelicae Gigantis Radix extract fermented with honey(SST) and $4.73\;{\pm}\;0.04%$ in Angelicae Gigantis Radix extract fermented with Nuruk(SNT)(p<0.05), respectively.

• The Korea Journal of Herbology
• /
• v.25 no.4
• /
• pp.7-10
• /
• 2010

Objectives : The purpose of this study was to investigate the nodakenin and decursin contents in each fermented Angelicae gigantis Radix by 4 species of ferment strains. Methods : The strains of fermented Angelicae gigantis Radix were Lactobacillus plantarum(SE1), L. acidophilus(AC), Bacillus subtilis(B2) and B. licheniformis(BL2). The fermentative changes of nodakenin and decursin were analyzed using HPLC. Results : All of 4 species strains reduced nodakenin and decursin concentration in Angelicae gigantis Radix by fermentation. But fermentability were difference between strains. The nodakenin concentration of AC was lower than B2. The decursin concentrations of SE1 and AC were lower than B2 and BL2. Conclusions : 4 species of fermental strain were difference decomposable rate with nodakenin and decursin in fermented Angelicae gigantis Radix.

• The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology
• /
• v.21 no.3
• /
• pp.10-19
• /
• 2008

Objective : To investigate the effects of Radix Ophiopogon japonieus on atopic dermatitis, I prepared DNCB(2,4-dinitrochlorobenzen) induced atopic dermatitis NC/Nga mice and observed the mice by four ways; eye observation, the number of skin behavior times, histological changes of skin and cytokine(Total IgE, IL-4, $IFN-{\gamma}$). Methods : After prepare Radix Ophiopogon japonicus extract, DNCB induced atopic dermatitis NC/Nga mice were divided into three groups. The first is Control group which was intact group. The second is Medication group which was orally medicated Radix Ophiopogon japonicus extract one time a day for consecutive 5 days. The third group is Application group which was applied Radix Ophiopogon japonicus extract externally one time a day for consecutive 5 days. After that, the effect of Radix Ophiopogon japonicus on atopic dermatitis was observed. Statistical analysis was performed by using Kmskal-Wallis test and statistical significance was set at less than 5%. Results : 1. Radix Ophiopogon japonicus showed some in both Medication group and Application At observation of skin morphologic change, effects to prevent erythema reaction on skin group. 2. At the number of scratching behavior times, Radix Ophiopogon japonicus showed an effect to decrease scratching behavior times, but there was no statistical significance among three groups. 3. At skin tissue H-E stain, Radix Ophiopogon japonicus showed an effect to prevent skin epidermal tissue damages and also showed that it could keep the skin healthy in both Medication group and Application group. Especially in Application group, the skin of mouse showed almost normal recovery. 4. At cytokines, there was no statistical significance among three groups in IgE and IL-4. But Radix Ophiopogon japonicus showed an significant effect to suppress $IFN-{\gamma}$ in both Medication group and Application group. There was no significant difference between two groups. Conclusion : Radix Ophiopogon japonicus has some effects on atopic dermatitis in both internal medication and external application.

• Journal of Pharmacopuncture
• /
• v.4 no.2
• /
• pp.87-93
• /
• 2001

Objectives : Through the literature on the effect of Aconiti Cliare Tuber, Radix Aconiti, we are finding out the clinical posibility and revealing the more effective to intractable disease. Methods : We inverstigated the literatures of Oriental Medicine and experimental reports about Aconiti Cliare Tuber, Radix Aconiti. Results : 1. The taste of Aconiti Cliare Tuber, Radix Aconiti is hot, sweet, bitter, warm and hot, and the effect is dehumidification, warm up and relieve the pain, so it can be used for arthritis, hemiplegia, carpopedal spasm, sciatica, cancer, numbness. 2. A toxic constituent of Aconiti Cliare Tuber, Radix Aconitiis is induced by aconitine alkaloid, develope toxic symptoms and result in death. So it needs suitabe treatment for safety. 3. It is known that the toxicopathy due to Radix Aconiti was 3-30g(dosage for adult) and Aconiti Ciliare Tuber was 1-9g. But only using aconitine alkaloid to oral feeding, the toxicopathy due to 0.2mg/kg and lethal dose is 3-4mg. So we using this for treating, we must be careful and need more varialble study about toxicopathy, lethal dose. 4. On clinical treatment, we thought Aconiti Cliare Tuber, Radix Aconiti is so effective to intractable disease after control the toxicity, it may be need variable study on toxicity and clinical effects.

• Journal of the Institute of Electronics Engineers of Korea SP
• /
• v.45 no.3
• /
• pp.103-110
• /
• 2008

In this paper, an efficient butterfly structure for 8K/2K-Point Radix-4 FFT algorithm using CORDIC(coordinate rotation digital computer) is proposed. It is shown that CORDIC can be efficiently used in twiddle factor calculation of the Radix-4 FFT algorithm. The Verilog-HDL coding results for the proposed CORDIC butterfly structure show 36.9% cell area reduction comparison with those of the conventional multiplier butterfly structure. Furthermore, the 8K/2K-point Radix-4 pipeline structure using the proposed butterfly and delay commutators is compared with other conventional structures. Implementation coding results show 11.6% cell area reduction. Due to its efficient processing scheme, the proposed FFT structure can be widely used in large size of FFT like OFDM Modem.

• Herbal Formula Science
• /
• v.14 no.1
• /
• pp.42-51
• /
• 2006

This study was investigated to make sure the range of Radix Asparagi treatment. the nature of disease. the pathology and the dosage of it in Dongeuybogam. The consequences were as follows: 1. The Radix Asparagi is used in 11 fields of treatment such as exhaustion, tussis and phlegm. blood diseases. etc 2. The Radix Asparagi is primarily used in the treatment of heart and lung disease, weak and infirm. and controlling hydrate and fever as well as tussis and phlegm. 3. The Radix Asparagi is used for nature of disease which of transmitting between sap and fever. cardiac disease caused by stress. floating fever. debility of blood, malfunction of heart and kidney. and deficiency of vitality. 4. The Radix Asparagi is used in a range of $2g{\sim}16g$ per dose with 4g being the typical dose for most treatments. 5. The Radix Asparagi is used as a fundamental prescription with several herb remedies for the pathology. The Radix Asparagi has been used to moisten the dryness and to keep the balance among fever of heart, lung, and kidney. According to the results. I suggest to use Radix Asparagi in a various pathogenic fields. The Radix Asparagi is able to use to improve not only fatigue. but also diabetes, cataract, and debility of blood.

• The Journal of Korean Medicine
• /
• v.23 no.4
• /
• pp.113-124
• /
• 2002

Objects: This research was conducted to investigate the protective effect of Bupleuri Radix against ischemic damage using PC12 cells and global ischemia in gerbils, Methods: To observe the protective effect of Bupleuri Radixon ischemic damage, viability and changes in activities of superoxide dismutase (SOD), glutathione peroxidase (GPx), catalase and production of malondialdehyde (MDA) were observed after treating PC12 cells with Bupleuri Radix during ischemic damage. Gerbils were divided into three groups: a normal group, a 5-minute two-vessel occlusion (2VO) group and a Bupleun Radix administered group after 2VO. The CCAs were occluded by microclip for 5 minutes, Bupleuri Radix was administered orally for 7 days after 2VO. Histological analysis was performed on the 7th day. For histological analysis, the brain tissue was stained with 1 % of cresyl violet solution. Results: 1. Bupleuri Radix has a protective effect against ischemia in the CA1 area of the gerbil's hippocampus 7 days after 5-minute occlusion. 2. In the hypoxia/reperfusion model using PC12 cells, the Bupleuri Radix has a protective effect against ischemia in the dose of 0.2{\;}\mu\textrm{g}/ml,2{\;}\mu\textrm{g}/ml{\;}and{\;} 20{\;}\mu\textrm{g}/ml$. 3. Bupleuri Radix increased the activities of glutathione peroxidase and catalase. 4. The increased activity of superoxidedismutase (SOD) by ischemic damage might have been induced as an act of self-protection. This study suggests that Bupleuri Radix has some neuroprotective effect against neuronal damage following cerebral ischemia in vivo with a widely used experimental model of cerebral ischemia in Mongolian gerbils. Bupleuri Radix also has protective effect on a hypoxia/reperfusion cell culture model using PC12 cells. Conclusions: Bupleuri Radix has protective effect against ischemic brain damage during the early stages of ischemia.