• Title/Summary/Keyword: $Poly({\beta}-hydroxybutyrate-co-3-hydroxyvalerate)$ Hydroxyvalerate content

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Poly$({\beta}-hydroxybutyrate-co-3-hydroxyvalerate)$의 생분해도에 미치는 hydroxyvalerate 함량의 영향

  • Im, Seol-Hui;Jo, Gyeong-Suk;Ryu, Hui-Uk;Choe, Hui-Sik
    • 한국생물공학회:학술대회논문집
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    • 2000.11a
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    • pp.175-178
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    • 2000
  • Biodegradability of the $poly({\beta}-hydroxybutyrate-co-3 -hydroxyvalerate)$ [PHB/V] containing 0, 10 and 15mol% hydroxyvalerate [HV] was studied. Bioderadability of PHB/V was evaluated at $30^{\circ}C$ for 58 days and $55^{\circ}C$ for 33 days by monitoring the time-dependent changes in weight loss(erosion) of aerobic conditions in a temperature-controlled microcosms containing the earthworm cast($30^{\circ}C$) and compost ($55^{\circ}C$). It was found that PHB/V biodegradability occurred with increasing HV monomer concentration from 0 mol% to 15 mol%.

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Isolation and Characterization of a Methylotroph Producing 3-hydroxybutyrate-3-hydroxyvalerate Copolymer

  • JUNG HOE KIM;KIM, PIL;SEON WON KIM;GYUN MIN LEE;HYUN SOO LEE
    • Journal of Microbiology and Biotechnology
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    • v.5 no.3
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    • pp.167-171
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    • 1995
  • A bacterial strain C-02 using methanol as a carbon source was isolated from Gumi Industrial Estate and selected based on its rapid growth and capability of poly-$\beta$-hydroxybutyrate accumulation. Characteristics of strain C-02 showed that it belongs to the Methylococcaceae family, Type II subgroup. Strain C-02 could incorporate valerate into the PHB chain to form 3-hydroxybutyrate and 3-hydroxyvalerate (P(3HB-co-3HV)). Among various nutrient limitation tests, the nitrogen limitation test resulted in the highest content of P(3HB-co-3HV) per dry cell weight, 50$%$. Under the nitrogen limited condition, the average molecular weight of P(3HB-co-3HV) obtained was determined to be approximately $2.8\times 10^5$ daltons.

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