• BMB Reports
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• v.45 no.6
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• pp.371-376
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• 2012

To investigate the therapeutic effect of a Korean herbal medicine Pulsatilla koreana as an anti-septic agent, anti-inflammatory effects of the herbal medicine were determined in lipopolysaccharide (LPS)-exposed rats. Treatment with a methanol extract from Pulsatilla koreana significantly inhibited LPS-induced inflammatory responses. Results from ELISA analysis showed that Pulsatilla koreana decreased the plasma and hepatic levels of pro-inflammatory cytokines such as IL-$1{\beta}$, IL-6, TNF-${\alpha}$ while increased the level of anti-inflammatory cytokine IL-10 in LPS-exposed rats. Pulsatilla koreana also decreased the plasma levels of other inflammatory mediators such as $NO_3{^-}/NO_2{^-}$, ICAM-1, $PGE_2$, and CINC-1 in LPS-exposed rats. Although no significant effects were observed in the phagocytic activities, the distribution of lymphocyte population was significantly shifted by the treatment with Pulsatilla koreana. All together, Pulsatilla koreana exerts anti-inflammatory activities in the immune-challenged animals implicating that this Korean herbal medicine is therapeutically useful for the treatment of inflammatory diseases like sepsis.

• Journal of Physiology & Pathology in Korean Medicine
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• v.27 no.4
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• pp.416-421
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• 2013

Cirsium japonicum var. ussuriense is often used in treatment of human disease such as hemorrhage, blood congestion and inflammation. The present study has been undertaken to investigate the effect of the leaf extract from C. japonicum var. ussuriense (CLE) on the development of collagen-induced arthritis (CIA) in DBA1/J mice. CLE administration suppressed markedly the arthritis incidence and arthritis score in CIA mice. Also, CLE significantly suppressed the release of $PGE_2$, TNF-${\alpha}$, IL-4 and IL-6 in CIA mice. However, CLE significantly increased the production of IL-10, but not IL-4. These results suggest that CLE suppress inflammatory mediators and regulates Th1 and Th2 cytokines. These properties may contribute to the anti-arthritis action of CLE.

• Korean Journal of Plant Resources
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• v.23 no.6
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• pp.487-497
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• 2010

ROS have been associated with pathogenic processes including carcinogenesis through direct effect on DNA and play an important role in the pathogenesis of inflammation. Because of many types of phenolic acid derivatives and flavonoids, apples have been one of the human diet since ancient times and are one of the most commonly consumed fruits in worldwide. In this study, catechin, chlorogenic acid and phlorizin dihydrate were purified and identified by HPLC and GC/MS. The contents of catechin, chlorogenic acid and phlorizin dihydrate were 1.01 mg, 7.01 mg and 3.67 mg/ kg wet weight, respectively. Catechin and phlorizin dihydrate were found to significantly inhibit oxidative DNA damage, while chlorogenic did not affect. Also, catechin inhibits NO and $PGE_2$ production via suppressing iNOS and COX-2 expression. However, chlorogenic acid and phlorizin dihydrate did not affect. Our results show that catechin may be the most active phenolic compound in anti-oxidative damage and anti-inflammatory effect.

• Journal of Oriental Neuropsychiatry
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• v.21 no.4
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• pp.41-51
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• 2010

Objectives : Samul-tang(SM). Sagunja-tang(SG). Pamul-tang(PM) and Sipjeondaebo-tang(SJ) was used many diseases such as sterility. menstrual disorder. general prostration. recruitment in Korea. We investigated the immune stimulatory activities of SG. SM. PM and SJ in in vitro. Methods: For comparision for effective of SM. SG. PM and SJ. this study examined anti-inflammation(NO. PGE2 assay). anti-oxidation(DPPH assay) and immune response in in vitro assay. For immune response activities. this study used NO synthesis on RAW 264.7 cells, splenocyte proliferation and cytokine assay(IL-2, IL-4) in splenocyte. Results: The results showed that SG. SM. PM and SJ were no significant effect anti-oxidation and anti-inflammatory effects. For immune response. they showed the splenocyte proliferation and macrophage proliferation. We confirmed that they synthesised NO a dose-dependent manner significantly and secreted the IL-4. Conclusions : These results suggested the SG, SM, PM and SJ had immune stimulatory activity. A efficacy of immune response in them had produced similar results.

• Journal of Physiology & Pathology in Korean Medicine
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• v.24 no.1
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• pp.61-66
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• 2010

Inflammation is a double-edge sword, functioning as a response against external stimuli, but excessive inflammation can lead to a harmful result. Inflammation is important in the development of vascular diseases, such as atherosclerosis and restenosis. Sohamhyung-tang(SHHT) is one of major herbal prescriptions from "Sanghanron", treating congested heat in thorax. It can be seen as an origin of several prescriptions for Hyungbi(胸痺, thoracic congestion), with its major component Trichosanthes semen. This study was done to determine whether SHHT reduces the production of nitrite, index of NO, and pro-inflammatory cytokines in Lipopolysaccharide(LPS)-induced RAW 264.7 cells. The results show that SHHT inhibited LPS-induced production of NO and PGE2, and iNOS and COX-2 expression, meaning SHHT has a significant anti-inflammatory effect. Based on these results, we suggest that SHTT can be used for prevention and treatment of diseases which starts from chronic inflammatory reactions.

• Journal of Food Hygiene and Safety
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• v.8 no.4
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• pp.181-188
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• 1993

It is well known that bacterial lipopolysaccharide (LPS) stimulates the prostaglandin (PG) synthesis in various experimental system, but the mechanism and the detailed nature of its action are yet to be understood. Thus, this study was designed to characterize LPS induced PG synthesis in rat alveolar macrophage. Although results were not so much prominent, LPS stimulated PGE2 synthesis in macrophage with short term exposure, and this was thought to be mainly due to the activation of phopholipase A2+ But there was a burst in the PG synthesis 6 hours after the LPS treatment and this was accompanied with the increase of cyclooxygenase activity. This effect was not mediated by tumor necrosis factor (TNF) or platelet activating factor (PAF), and the existence of serum was prerequisite for its action. Growth factors such as epidermal growth factor (EGF) and platelet derived growth factor (PDGF) themselves did not stimulate PG synthesis and the showed stimulatory activities to some extent. Normal rat serum was more effective for the elicitation of the LPS action than growth factors. Thus, considering the amounts of growth fafctors contained in normal serum, it was suggested that another factors like LPS binding protein (LBP) might be involved in the serum effect on LPS action. Conclusively. it was thought that LPS could stimulate PG synthesis through interaction with serum factors such as EGF, PDGF and/or LBP.

• Archives of Pharmacal Research
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• v.29 no.2
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• pp.165-171
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• 2006

Osteoarthritis is thought to be induced by the ageing-related loss of homeostatic balance between degeneration and repair mechanism around cartilage tissue in which inflammatory mediators such as reactive oxygen species, cytokines and prostaglandins are prone to overproduction under undesirable physiological conditions. Phlorotannins are unique polyphenolic compounds bearing dibenzo-1,4-dioxin skeleton which are not found in terrestrial plants but found only in some brown algal species such as Ecklonia and Eisenia families. Phlorotanninrich extracts of Ecklonia cava including LAD103 showed significant antioxidant activities such as DPPH radical scavenging, ferric ion reduction, peroxynitrite scavenging, and inhibition of LDL oxidation, indicating their possible antioxidative interference both in onset and downstream consequences of osteoarthritis. LAD103 also showed significant down regulation of $PGE_2$ generation in LPS-treated RAW 246.7 cells, and significant inhibition of human recombinant interleukin-$1{\alpha}$-induced proteoglycan degradation, indicating its beneficial involvement in pathophysiological consequences of osteoarthritis, the mechanism of which needs further investigation. Since LAD103 showed strong therapeutic potentials in arthritic treatment through several in vitro experiments, it is highly encouraged to perform further mechanistic and efficacy studies.

• Journal of Acupuncture Research
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• v.22 no.2
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• pp.133-139
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• 2005

Cobrotoxin, a venom of Vipera lebetina turanica, is a group of basic peptidescomposed of 233 amino acids with six disulfide bonds formed by twelve cysteins. NF-kB is activated by subsequent release of inhibitory IkB and translocation of p50. Since sulfhydryl group is present in kinase domain of p50 subunit of NF-kB, cobrotoxin could modify NF-kB activity by protein-protein interaction. We therefore examined effect of cobrotoxin on NF-kB activities in lipopolysaccharide (LPS) and sodium nitroprusside (SNP)-stimulated Raw 264.7 mouse macrophages. Cobrotoxin suppressed the LPS and SNP-induced release of IkB and p50 translocation resulted in inhibition of DNA binding activity of NF-kB. Inhibition of NF-kB resulted in reduction of the LPS and SNP-induced production of inflammatory mediators NO and PGE2 generation. The inhibitory effect of cobrotoxin on the NF-kB activity were blocked by addition of reducing agents dithiothreitol and glutathione. These results demonstrate that cobrotoxin inhibits activation of NF-kB, and suggest that pico to nanomolar range of cobrotoxin could inhibit the expression of genes in the NF-kB signal pathway.

• Journal of The Korean Society of Grassland and Forage Science
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• v.12 no.3
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• pp.70-73
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• 1992

사료작물 저온발아성(低溫發芽性)은 수수 $\times$ 수단그라스잡종들이 좋았고 옥수수와 수단그라스는 비교적 저조하였고 PGE에 종자침지처리가 이탈리안라이그라스의 저온발아에 효과적이었다. 간척지의 수수파종시 염해 방지에는 모래를 파종후 피복하여 제염(除鹽) 효과를 얻을수 있었다. 수수류의 토양수분조건에 대한 광합성 연구는 포장용수량의 $60\sim80%$의 조건에서 광합성이 가장 왕성하였고 건물수량도 동일한 결과를 나타내었다. 옥수수의 광이용과 초형과의 관계연구는 이삭상부 엽각의 직립화는 밀식 조건에서 광이용성이 증가되었고 건물 및 종실수량에서 종수가 있었다. 또 밀식화에 따라 직립화 경향이 현저하였으며 옥수수의 다수성초형의 육성이 요청되었다. 질소시비에 대한 청예류의 생장 반응 연구들은 수단그라스에서 10a당 40Kg까지는 건물수량생산을 증가시켰으나 80Kg/10a 사용시는 질산태질소함량이 중독위험치를 초과하였다. 이러한 질산태질소의 중독위험은 6엽기까지 존재하였고 청산중독의 위험농도는 4엽기 이전까지, 또 18/$8^{\circ}C$의 저온조건에서 나타났다. 옥수수의 적정재식 밀도는 10a당 16.600본까지 밀식할수록 증가되었으나 가소화수량과 종실수량은 5.500본과 10Kg/10a 에서 최고치를 나타내었고 질소 추비 효과는 대관령 같은 고산지대에서는 나타나지 않았다. 맥류의 총체(總體)사료 이용성은 호밀, 밀, 보리 순(順)으로 저하하였고 파종시기가 빠를수록 월동성이 향상되었으며 수확적기는 출수후 24일경이었다. 호밀의 청예이용에는 월동후 2회예취와 12cm높이가 건물수량과 TDN수량을 높힐 수 있었다. 수수$\times$ 수단잡종의 출수기 이전 예취는 고사 경비율을 줄일 수 있었고 년 2회 예취와 예취높이 10cm가 가장 좋았다. 유채는 생육기간이 길어질수록 수량은 증가하였고 소화율의 감소도 현저하지 않았다.

• Biomolecules & Therapeutics
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• v.14 no.4
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• pp.216-219
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• 2006

In order to establish the anti-inflammatory cellular mechanism of the paeony root(Paeonia lactiflora, Pall, Paeoniaceae), the constituents including paeoniflorin, albiflorin, (+)-catechin, paeonol, benzoic acid and methyl gallate were evaluated for their effects on arachidonate and NO metabolism. Among the compounds tested, only paeonol weakly inhibited cyclooxygenase-2-mediated $PGE_2$ production from LPS-treated RAW 264.7 cells. (+)-Catechin and methyl gallate weakly inhibited inducible nitric oxide synthase-mediated NO production from the same cell line. In particular, methyl gallate significantly inhibited 5-lipoxygenase from RBL-l cells with an $IC_{50}$ of 8.4 ${\mu}M$. These results suggest that the inhibition of these components on arachidonate and NO metabolism may contribute at least in part to anti-inflammatory mechanism of the paeony root.