• 한국어병학회지
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• 제35권1호
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• pp.27-40
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• 2022

2019년부터 2020년도까지 국내로 수입 및 유통되고 있는 냉동 새우에 대하여 갑각류 전염병인 전염성피하및조혈기괴사증(infectious hypodermal andhematopoietic necrosis, IHHN), 노란머리병(yellow head disease, YHD), 타우라증후군(taura syndrome, TS), 전염성근괴사증(infectious myonecrosis, IMN)의 병원체 유전자 검출 여부를 조사하였다. Polymerase chain reaction (PCR) 분석을 수행한 결과 5개 국가에서 수입된 총 10개 그룹의 시료에서 YHV (1/10), IHHNV (2/10)가 검출되었으며 TSV와 IMNV는 검출되지 않았다. 또한, 검출된 IHHNV의 경우 type A IHHNV related gene을 증폭시킬 수 있는 MG831F/R primer를 이용한 PCR 결과에서도 양성으로 확인되었다. 유전자가 검출된 시료에 대해 염기서열 및 계통학적 분석 결과 YHV는 genotype 3 (YHV3)로 분류되었으며 IHHNV는 infectious IHHNV type 2 로 분류되었다. PCR 양성 조직 시료를 마쇄 후 흰다리새우에 인위 감염 실험을 수행한 결과, 폐사는 나타나지 않았으나 접종한 바이러스 유전자는 검출되었다. 이러한 결과는 YHV3와 IHHNV가 냉동 새우의 수입 과정에서 국내로 유입되고 있음을 시사한다. 본 연구에서 검출된 YHV3와 IHHNV는 병원성이 없는 유전형 또는 수입 및 유통 과정에서 불활화에 따라 흰다리새우에 폐사를 유발하기에는 감염성이 낮은 수준의 형태로 존재하고 있을 것으로 사료된다.

• Animal Bioscience
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• 제36권3호
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• pp.484-491
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• 2023

Objective: The aim of this trial was to investigate the effect of supplementation and withdrawal of selenium-enriched kale sprouts (SeKS) on productivity and egg Se concentration of laying hens. Selenium from commercial Se-enriched yeast (SeY) was used as a comparative Se source. Methods: One-hundred and eighty 61-week-old laying hens were randomly divided into 5 treatment groups with 4 replicates (9 hens each) in a 2×2+1 Augmented Factorial Experiment in a completely randomized design. The experimental diets were basal diet, basal diet supplemented with 0.2 and 0.4 mg Se/kg from SeKS and SeY, respectively. The 8-week feeding trial was divided into 2 periods, namely the Se supplemental period (week 1 to 4) and the Se withdrawal period (week 5 to 8). Results: Productive performance, egg quality and egg Se concentration of laying hens were not affected by sources of Se (SeKS and SeY) during both, the Se supplemental and withdrawal periods. Egg production and egg Se concentration increased (p<0.05) with increasing levels of Se supplementation. The egg Se concentration increased and reached a peak 1 week after Se supplementation. However, concentration of Se in eggs of hens fed Se from both sources decreased rapidly from the second week of the Se withdrawal period to reach the same egg Se concentration of hens fed the basal diet by the fourth week of the Se withdrawal period. Conclusion: The efficacy of Se from SeKS on productivity and egg Se concentration in laying hens was comparable to commercial SeY. Thus, SeKS can provide an alternate organic Se source for production of Se-enriched eggs.

• 한국엔터테인먼트산업학회논문지
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• 제13권1호
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• pp.199-206
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• 2019

연구목적은 동작관찰훈련이 뇌졸중 환자의 인지기능 향상에 영향을 미치는가를 알아보고자 하였다. 연구방법으로는 2018년 4월 23일부터 5월 18일까지 경인지역 병원에 입원 중인 재활환자 7명을 대상으로 MMSE-K와 LICA를 사용하여 평가를 진행하였고, 동작관찰프로그램으로는 상지 ROM 댄스, 풀 오버형 옷정리, 운동화 끈 묶기, 수건 개기, 양말 개기, 종이접기, 넥타이 매는 법 총 7가지 과제를 적용하였다. 통계프로그램으로는 SPSS version 20을 사용하였으며 기술통계, Wilcoxon signed ranks을 사용하여 값을 구하였다. 동작관찰훈련을 적용한 결과 MMSE-K 점수는 3.29±1.38의 변화량을 보여 p값 0.001로 유의한 값을 보였고, LICA 점수는 기억력에서 12.16±6.73으로 가장 유의한 값이 나왔다. 결론은 동작관찰훈련이 뇌졸중 환자의 인지기능 영역 중 기억력에서 가장 많은 영향을 미쳤으며, 다른 영역에서도 점수 향상은 있었으나 통계적으로 유의하지는 않았다. 앞으로 동작관찰훈련이 많이 사용되어 추후에는 뇌손상 부위별로 어느 영역에서 효과가 있는지에 대한 후속 연구가 이루어지길 바란다.

• 한국발생생물학회:학술대회논문집
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• 한국발생생물학회 2003년도 제3회 국제심포지움 및 학술대회
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• pp.84-84
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• 2003

DNA methylation is involved in epigenetic processes such as X-chromosome inactivation, imprinting and silencing of transposons. DNA methylation is a highly plastic and critical component of mammalian development The DNA methyltransferases (Dnmts) are responsible for the generation of genomic methylation patterns, which lead to transcriptional silencing. The maintenance DNA methyltransferase enzyme, Dnmt 1, and the de novo methyltransferase, Dnmt3a and Dnmt3b, are indispensable for development because mice homozygous for the targeted disruption of any of these genes are not viable. The occurrence of DNA methylation is not random, and it can result in gene silencing The mechanisms underlying these processes are poorly understood. It is well established that DNA methylation and histone deacetylation operate along a common mechanistic pathway to repress transcription through the action of methyl-binding domain proteins (MBDs), which are components of, or recruit, histone deacetylase (HDAC) complexes to methylated DNA. As a basis for future studies on the role of the DNA-methyl-transferase in porcine development, we have isolated and characterized a partial cDNA coding for the porcine Dnmt1. Total RNA of testis, lung and ovary was isolated with TRlzol according to the manufacture's specifications. 5 ug of total RNA was reverse transcribed with Super Script II in the presence of porcine Dnmt 1 specific primers. Standard PCRs were performed in a total volume of 50 ul with cDNA as template. Two DNA fragmenets in different position were produced about 700bp, 1500bp and were cloned into pCR II-TOPO according to the manufacture's specification. Assembly of all sequences resulted in a cDNA from 158bp of 5'to 4861bp of 3'compare with the known human maintenance methyltransferase. Now, we are cloning the unknown Dnmt 1 region by 5'-RACE method and expression of Dnmt 1 in tissues from adult porcine animals.

• 대한본초학회지
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• 제24권4호
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• pp.115-120
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• 2009

Objectives : Due to the morphological similarity and frequent occurrence of intermediate forms as well as morphological variations of aerial part, the correct identification between Rhei Radix et Rhizoma and Rhei Undulatai Rhizoma is very difficult. To develop a reliable method for correct identification and improving the quality standards of Rhei Radix et Rhizoma and Rhei Undulatai Rhizoma, we analyzed RAPD and developed SCAR marker. Methods : To amplify target DNA at the genomic level, 32 Operon 10-mer random primers were applied with four Rheum species, R. officinale, R. palmatum, R. tanguticum and R. undulatum. The nucleotide sequences were determined and species-specific primers were prepared depending on the species-specific RAPD amplicons after subcloned into the pGEM-Teasy vector. To develop the SCAR markers, species-specific PCR amplification and multiplex-PCR were carried out using the single species-specific primer pairs and combinations of them, respectively. Results : We used RAPD analysis of four Rheum plant species to obtain several species-specific RAPD amplicons. From nucleotide sequences of these RAPD amplicons, we developed two SCAR markers that amplified 314 bp and 390 bp DNA fragments in only R. undulatum but not in R. officinale, R. palmatum, R. tanguticum and R. undulatum, for distinguishing Rhei Undulatai Rhizoma and Rhei Radix et Rhizoma. Furthermore, we established SCAR markers for the simultaneous discrimination of the three species within a single reaction by using multiplex-PCR. Conclusions : These genetic markers can be used for the efficient discrimination of plants species and commercial herbal medicines between Rhei Undulatai Rhizoma and Rhei Radix et Rhizoma, to ultimately prevent indiscriminate distribution and prescription of these herbal medicines.

• 한국지반공학회논문집
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• 제24권2호
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• pp.87-97
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• 2008

단단한 모래 입자와 연약한 고무 입자로 이루어진 Engineered soils을 고결화 시킨 후 $K_o$ 상태에서의 거동 특성을 분석하였다. 고결화 효과에 따른 영향 및 모래부피비에 따른 영향을 파악하기 위하여 다양한 모래부피비를 가지는 비고결화 및 고결화 시료를 준비하여, 수직 응력에 따른 변형 및 탄성파 속도를 측정하였다. 탄성파 속도 측정은 벤더 엘리먼트와 PZT 엘리먼트를 이용하였다. 고결화 이 후 응력에 따른 수직 변형율의 기울기는 이중 선형 관계를 보이며 고결화 결합 파괴 이후에는 비고결화 시료와 비슷한 기울기를 가진다. 정규화된 수직 변형량은 응력에 따라 capillary force, cementation, decementation 구간으로 나눌 수 있다. 근접장 내에서 측정된 전단파 신호의 첫 번째 움직임은 압축파의 도달과 일치하였다. 고결화에 의해 탄성파 속도는 수직 응력의 증가 없이 급격한 증가를 보였으며, 고결화 이후 추가적인 응력 증가에도 일정한 값을 보였다. 고결화 파괴 후 지속적인 수직 응력의 증가에 따라 탄성파속도는 증가하였다. 고결화는 비고결화 시료에서 나타나는 유사고무, 유사모래, 전이 3가지의 거동을 방해한다. 고무-모래 혼합재의 고결화 결합의 파괴 메커니즘은 모래부피비에 따라 다르며 낮은 모래부피비의 시료는 입자 모양의 변화가, 높은 모래부피비 시료에서는 입자 구조의 변화가 고결화 결합의 파괴가 주요한 원인이다. 본 연구를 통해 연약한 고무 입자와 단단한 모래 입자의 혼합재인 Engineered soils의 거동은 고결화 및 고결화 파괴에 따라 비고결화 시료와 구분됨을 알 수 있었다.

• Journal of the Korean Association of Oral and Maxillofacial Surgeons
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• 제49권3호
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• pp.125-134
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• 2023

Objectives: The aim of the study was to quantify and compare craniofacial asymmetry in subjects with and without symptoms of temporomandibular joint disorders (TMDs). Materials and Methods: A total of 126 adult subjects were categorized into two groups (63 with a TMDs and 63 without a TMDs), based on detection of symptoms using the Temporomandibular Joint Disorder-Diagnostic Index (TMD-DI) questionnaire. Posteroanterior cephalograms of each subject were traced manually and 17 linear and angular measurements were analyzed. Craniofacial asymmetry was quantified by calculating the asymmetry index (AI) of bilateral parameters for both groups. Results: Intra- and intergroup comparisons were analyzed using independent t-test and Mann-Whitney U test, respectively, with a P<0.05 considered statistically significant. An AI for each linear and angular bilateral parameter was calculated; higher asymmetry was found in TMD-positive patients compared with TMD-negative patients. An intergroup comparison of AIs found highly significant differences for the parameters of antegonial notch to horizontal plane distance, jugular point to horizontal plane distance, antegonial notch to menton distance, antegonial notch to vertical plane distance, condylion to vertical plane distance, and angle formed by vertical plane, O point and antegonial notch. Significant deviation of the menton distance from the facial midline was also evident. Conclusion: Greater facial asymmetry was seen in the TMD-positive group compared with the TMD-negative group. The mandibular region was characterized by asymmetries of greater magnitude compared with the maxilla. Patients with facial asymmetry often require management of temporomandibular joint (TMJ) pathology to achieve a stable, functional, and esthetic result. Ignoring the TMJ during treatment or failing to provide proper management of the TMJ and performing only orthognathic surgery may result in worsening of TMJ-associated symptoms (jaw dysfunction and pain) and re-occurrence of asymmetry and malocclusion. Assessments of facial asymmetry should take into account TMJ disorders to improve diagnostic accuracy and treatment outcomes.

• Animal Bioscience
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• 제35권9호
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• pp.1327-1339
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• 2022

Objective: Fat deposition in poultry is an important factor in production performance and meat quality research. miRNAs also play important roles in regulating adipocyte differentiation process. This study was to investigate the expression patterns of miRNAs in duck adipocytes after differentiation and explore the role of miR-214 in regulating carnitine palmitoyltransferases 2 (CPT2) gene expression during duck adipocyte differentiation. Methods: Successful systems for the isolation, culture, and induction of duck primary fat cells was developed in the experiment. Using Illumina next-generation sequencing, the miRNAs libraries of duck adipocytes were established. miRanda was used to predict differentially expressed (DE) miRNAs and their target genes. The expression patterns of miR-214 and CPT2 during the differentiation were verified by quantitative real-time polymerase chain reaction and western blot. Luciferase reporter assays were used to explore the specific regions of CPT2 targeted by miR-214. We used a miR-214 over-expression strategy in vitro to further investigate its effect on differentiation process and CPT2 gene transcription. Results: There were 481 miRNAs identified in duck adipocytes, included 57 DE miRNA candidates. And the 1,046 targets genes of DE miRNAs were mainly involved in p53 signaling, FoxO signaling, and fatty acid metabolism pathways. miR-214 and CPT2 showed contrasting expression patterns before and after differentiation, and they were selected for further research. The expression of miR-214 was decreased during the first 3 days of duck adipocytes differentiation, and then increased, while the expression of CPT2 increased both in the transcriptional and protein level. The luciferase assay suggested that miR-214 targets the 3'untranslated region of CPT2. Overexpression of miR-214 not only promoted the formation of lipid droplets but also decreased the protein abundance of CPT2. Conclusion: Current study reports the expression profile of miRNAs in duck adipocytes differentiated for 4 days. And miR-214 has been proved to have the regulator potential for fat deposition in duck.

• 한국환경보건학회지
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• 제49권5호
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• pp.251-261
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• 2023

Background: This paper deals with the study of natural radioactivity in rocks from Ogun State in Southwestern Nigeria. The aim is to determine radiation emissions from rocks in order to estimate radiation hazard indices. Objectives: The following objectives were targeted: 1. To determine radiation emissions from each type of rocks; 2. To estimate radiation hazard indices based on the rocks; 3. To correlate the activity concentrations of radionuclides with major oxides. Methods: The samples were analyzed using a NaI (Tl) gamma ray spectrometric detector and PerkinElmer AAnalyst 400 AAS spectrometer. Results: The activity of ⁴⁰K, ²²⁶Ra, and ²³²Th were found in order of decreasing magnitude from pegmatite>granite>migmatite. In contrast, lower concentrations were found in shale, phosphate, clay stone, sandstone and limestone. The mean absorbed doses were 125±23 nGyh^-1 (migmatite), 74±13 nGy/h (granite), 72±13 nGyh^-1 (pegmatite), 64±09 nGyh^-1 (quartzite), 45±16 nGyh^-1 (shale), 41±09 nGyh^-1 (limestone), 41±11 nGyh^-1 (clay stone), 24±03 nGyh^-1 (phosphate), and 21±10 nGyh^-1 (sandstone). The outdoor effective dose rates in all rock samples were slightly higher than the world average dose value of 0.34 mSvy^-1. The percentage composition of SiO₂ in the rock samples was above 50 wt% except for in the limestone, shale and phosphate. Al₂O₃ ranged from 4.10~21.24 wt%, Fe₂O₃ from 0.39~7.5 wt%, and CaO from 0.09-46.6 wt%. In addition, Na₂O and K₂O were present in at least 5 wt%. Other major oxides, including TiO₂, P₂O₅, K₂O, MnO, MgO and Na₂O were depleted. Conclusions: The findings suggest that Ogun State may be described as a region with elevated background radiation. It is recommended that houses should be constructed with good cross ventilation and residences should use home radiation monitoring instruments to monitor radon emanating from walls.

• 대한족부족관절학회지
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• 제27권4호
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• pp.123-130
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• 2023

Purpose: "Hallux valgus" is a common disease encountered in clinical practice and is accompanied by foot deformities. Conservative treatment is commonly used in the early stages of hallux valgus. On the other hand, surgical treatment often becomes necessary as the deformity progresses. Surgical treatments involve various osteotomy methods or joint fusion procedures combined with soft tissue release, and outcomes from these surgical treatments are generally favorable. This study compared two soft tissue release techniques in the hallux region. Materials and Methods: This study conducted a retrospective cohort study on 48 participants who underwent surgical treatment for hallux valgus at a single institution from March 1, 2018, to March 31, 2023. A scarf osteotomy was performed in all cases, and the "Modified Mcbride procedure" or "Trans-articular approach" was done for soft tissue release. Hallux valgus angle (HVA), intermetatarsal angle (IMA), and the degree of subluxation of the lateral sesamoid were measured through simple foot radiographs taken before surgery and one year after surgery. Results: In the Modified Mcbride procedure group, HVA, IMA, and the sesamoid position grade decreased from 34.94° to 9.98°, 15.64° to 5.44°, and 2.47 to 0.44, respectively. In the trans-articular approach group, HVA, IMA, and the sesamoid position grade decreased from 33.42° to 7.34°, 15.06° to 6.03°, and 2.17 to 0.58, respectively. There was no significant difference in these changes between the preoperative and one-year postoperative measurements for both techniques (p-value>0.05). Conclusion: A radiological assessment of soft tissue release through the Modified Mcbride procedure and trans-articular approach in hallux valgus did not show significant differences. Therefore, both surgical techniques can be considered in the distal soft tissue release for a hallux valgus correction.