• The Korean Journal of Ecology
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• 제21권5_1호
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• pp.457.1-463
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• 1998

the research has been made for alkali and metal element concentrations in top soils and plants from the abandoned coal mine, Keumsan, Chungnam Province. Samples of the top soil and plant (Miscanthus sinensis and Pinus rigida) were collected from the mine area in which was divided into t재 regions the polluted region influenced by the coal mining and the non-polluted region. pH of the top soils was 3.16-4.33 in the polluted region. Ca, Sr and P concentrations were high in the polluted soils, and Al and Ba concentrations were high in the non-polluted soils. No differences were found in K, Na and Ti concentrations. M. sinensis was higher in the element concentrations than P. rigida. In the average concentration of the alkali and metal element, M. sinensis showed high Cs and Na in the polluted region, and high Ba, Ca, K, Sr and concentrations in the non-polluted region. P. rigida had high Cs, Na and Rb concentrations in the polluted region. M. sinensis and P. rigida were higher in the root than above-ground part in the most element, but Ca and K. Ca, K and Na concentrations within both plants had higher than those of soils.

• Journal of Microbiology
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• 제37권4호
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• pp.229-233
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• 1999

The mitochondrial plasmid pMLP1 from a white-rot fungus, Pleurotus ostreatus, is a double-stranded DNA containing 381 bp terminal inverted repeat (TIR) whose 5'-ends are covalently bound by terminal proteins. The plasmid contains two major open reading frames (ORFs), encoding putative DNA and RNA polymerases, and a minor ORF encoding a small, highly basic protein. To identify the DNA binding activity that recognizes the TIR region of pMLP1, gel retardation assays were performed with mitochondrial extracts. A specific protein binding to a region between 123 and 248 nt within TIR was observed. We examined whether the gene product of mORF1 bindes to this region specifically. E. coli cell extract which contains an overproduced mORF1 protein formed a complex specific to the region between 123 and 248 nt. Inclusion of mORF1 protein in the specific complex formed between P. ostreatus mitochondrial extract and TIR was confirmed by a supershift assay using polyclonal antibodies against the mORF1 protein. Our result suggest that the product of mORF1 may function as a terminal region recognition factor (TRF), recognizing an internal region in TIR.

• Journal of Microbiology and Biotechnology
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• 제16권1호
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• pp.118-125
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• 2006

The region responsible for replication of the megaplasmid pAtC58 in the nopaline-type Agrobacterium tumefaciens strain C58 was determined. A derivative ofa Co1E1 vector, pBluscript SK-, incapable of autonomous replication in Agrobacterium spp, was cloned with a 7.6-kb Bg1II-HindIII fragment from a cosmid clone of pAtC58, which contains a region adjacent to the operon for the utilization of deoxyfructosyl glutamine (DFG). The resulting plasmid conferred resistance to carbenicillin on the A. tumefaciens strain UIA5 that is a plasmidfree derivative of C58. The plasmid was stably maintained in the strain even after consecutive cultures for generations. Analysis of nested deletions of the 7.6-kb fragment showed that a 4.3-kb BglII-XhoI region sufficiently confers replication of the derivative of the ColE1 vector on UIA5. The region comprises three ORFs, which have high homologies with repA, repB, and repC of plasm ids in virulent Agrobacterium spp. including pTiC58, pTiB6S3, pTi-SAKURA, and pRiA4b as well as those of symbiotic plasmids from Rhizobium spp. Phylogenie analysis showed that rep genes in pAtC58 are more closely related to those in pRiA4 than to pTi plasmids including pTiC58, suggesting that the two inborn plasmids, pTiC58 and pAtC58, harbored in C58 evolved from distinct origins.

• 대한환경공학회지
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• 제38권5호
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• pp.228-235
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• 2016

아질산화 반응을 통한 nitrite 축적은 단축질소제거 혹은 anammox 공정 수립을 위해 필수적이고 이 반응의 속도가 전체 질소제거공정의 효율에 큰 영향을 미칠 수 있다. 본 연구는 부유 미생물 연속류 반응기에서 pH 농도가 암모니아 폐수(2,000 mgN/L) 처리에 주는 복잡하고 다양한 영향 들을 modeling과 실험을 통해 종합적으로 분석하였다. modeling 연구 결과 반응의 안정성(stability)은 pH에 의해 지대한 영향을 받으며, free ammonia 저해가 심해지는 알칼리성 환경일수록 안정적 운전 영역(stable region)은 축소되었다. 기질과 pH의 좌표 상에서 stable region과 unstable region을 가르는 경계(stability ridge) 근처에서 안정적인 최대반응속도를 얻을 수 있고, 이 운전조건에서 아질산 축적 가능성도 최대가 되었다. stability ridge 근처의 조건에서 반응기를 운전한 결과 아질산화속도는 안정적으로 약 $6kgN/m^3-d$까지 얻을 수 있었고, 아질산축적율은 약 99% 이었다. 그러나 unstable region에서는 부하증가를 통한 반복된 교란 결과 유출수 암모니아 농도가 회복 불가능한 상태로 상승하였다. Modeling 결과 고유(intrinsic) 최적 pH 값을 고정하여도 실험에서 관찰되는 최적 운전 pH는 사용 기질의 농도가 높을수록 낮아지는 것으로 나타났으며, 이는 문헌에서 보고된 경향과 일치 하였다. 본 연구의 modeling 조건에서 95% 아질산화(5%는 암모니아로 잔존)를 위한 최적 운전 pH는 ~8.0인 것으로 예측되었으나, anammox 유입수 생산을 위해 55% 아질산화하려 할 때의 최적 운전 pH는 ~7.2로 낮아 졌다.

• 한국임상수의학회지
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• 제25권2호
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• pp.90-95
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• 2008

One fresh equine cadaver (two forelimbs) and five non-lamed thoroughbreds (ten sound forelimbs) were examined ultrasonographically through 5.0 MHz linear array transducer with a stand-off pad in palmar pastern region. The normal transverse ultrasonographic images of the superficial digital flexor tendon (SDFT), deep digital flexor tendon (DDFT), straight sesamoidean ligament (SSL), oblique sesamoidean ligament (OSL), and medium scutum could be identified at the region. The mean $\pm$ SD (min.$\sim$max. $mm^2$) of SDFT cross-sectional areas at P1A, P1B, P1C in the region were $110.00{\pm}5.38$ ($100{\sim}128$), $100.00{\pm}5.02$ ($90{\sim}111$), $114.00{\pm}3.33$ ($104{\sim}124$), respectively. The mean $\pm$ SD (min.$\sim$max. $mm^2$) of DDFT cross-sectional areas at each phalanx (P1A, P1B, P1C, P2A, P2B) were $136.00{\pm}4.83$ ($125{\sim}147$), $94.00{\pm}5.43$ ($85{\sim}108$), $99.00{\pm}4.87$ ($90{\sim}111$), $115.00{\pm}3.67$ ($108{\sim}124$), $135.00{\pm}3.65$ ($125{\sim}145$), respectively. The mean ratio of SDFT of P1B to DDFT was 0.74, 1.06, 1.01, 0.87, 0.74 at P1A, P1B, P1C, P2A, P2B, respectively.

• 미생물학회지
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• 제25권4호
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• pp.262-273
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• 1987

pBR 322와 pBD9을 이용하여 Staphylococcus aureus에서 분리된 chloramphenicol 저항성(Cmr) plasmid인 pSBK 203상의 ori 부위를 cloning하였다. 또한 E. coli 내에서도 발현하는 pSBK 203상의 Cm 저항성 부위 및 cloning 된 ori 부위를 pBR 322에 재조합시켜 E. coli와 그람양성균인 Bacillus subtilis 양쪽 모두에서 복제되고 또 항생물질에 대한 저항성도 각각 발현되는 shuttle vector 구성을 시도하였다.

• 한국콘텐츠학회논문지
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• 제12권8호
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• pp.47-58
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• 2012

본 논문에서는 MP2P(Mobile Peer-to-Peer) 네트워크 환경에서 이동하는 피어들을 대상으로 실시간으로 질의 결과를 갱신하는 연속적인 k-최근접 질의 처리 기법을 제안한다. 제안하는 기법은 질의 피어와 근접한 k개의 피어를 효율적으로 모니터링하기 위하여 모니터링 영역(monitoring region)을 생성하여 배포한다. 모니터링 영역은 주변 피어들의 이동 벡터를 이용하여 특정 시간 범위 내에서 최소 k개의 질의 결과를 보장하기 위해 만들어진 영역이다. 제안하는 기법은 주변 피어들의 벡터를 이용하여 Monitoring Region을 계산하기 때문에 유지 시간을 증가시켜 모니터링 영역의 무효화에 따른 질의 재수행 비용을 감소시킨다. 제안하는 기법의 우수성을 입증하기 위해 기존 기법과 성능 평가를 수행한 결과 제안하는 기법이 기존 기법보다 우수한 성능을 보였다.

• 전기의세계
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• 제27권4호
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• pp.45-51
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• 1978

A new method of realizing the field effect transistor with a sub-.mu. channel width is described. The sub-.mu. channel width is made possible by etching grooves into n$^{+}$ pn$^{[-10]}$ n$^{[-10]}$ structure and using p region at the wall for the channel region of the Metal-Oxide-Semiconductor transistor (MOST), or by diffusing two different types of impurities through the same diffusion mask and using p region at the surface for the channel region of MOST. When the drain voltage is increased at the pn$^{[-10]}$ drainjunction the depletion layer extends into the n$^{[-10]}$ region instead of into p region; this is also the secret of success to realize the sub-.mu. channel width. As the result of the experimental fabrication, a microwave MOST was obtained. The cut-off frequency was calculated to be 15.4 GHz by Linvill's power equation using the measured capacitances and transconductance.

• Journal of Preventive Medicine and Public Health
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• 제27권2호
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• pp.230-241
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• 1994

Though people occupationally exposed to machineries and automation in the industrialized society desire work involving decreased strength, due to the continuous and repetitive activities, a new industrial stress is present. Studies on prevalence of musculoskeletal disease and their related risk factors have evolved. In this study in relation to work tasks, we investigated the differences in musculoskeletal symptoms occurring In each body region. The results of the survey were as follows. 1. When comparing age, level of education, work duration, job satisfaction and leisure time activities according to work task, age in control group was $38.83{\pm}5.5$, in comparison to the other 2 groups was smaller(p<0.05), and level of education in control group was higher (p<0.05). Work duration in the cutting department was $8.04{\pm}4.99$ years longer than the other 2 groups (p<0.05), but there was no difference in the job satisfaction and leisure time activities. 2. The mean of symptom scores of each work task was 1.54 in the cutting department, 1.57 in the press department and 1.59 in the control group, and there was no significant differnce in the 3 groups. The mean of symptom scores for upper extremities in the control group was low but no statistically significant diffrence was shown. 3. When comparing the mean of symptom score according to work task in the each body region, in the shoulder region, the symptom score in the press department which desired strength was higher than the other 2 groups but no significant difference was shown. In the wrist region the cutting department scored 1.01 and in comparison to the other 2 groups was significantly increased (p<0.05). 4. The results of the univariate regression analysis on the major individual risk factors associated with musculoskeletal symptom relating work showed that previous symptom complaints in the same body region was significant risk factor(p<0.001) in the whole body Besides wrist, hip, and knee, psychological problem was shown to be a significant factor(p<0.05). And the body regions which work task was significant risk factor were wrist and neck region (p<0.05). 5. The results of the multiple regression analysis involving significant factors of each body region from the univariate regression analysis showed that previous symptom complaint in whole body region(p<0.001) and psychological problem in the shoulder, elbow and lumbar region (p<0.05) were significant factors, and work task was significant factor in the wrist (p<0.05).

• Asian Pacific Journal of Cancer Prevention
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• 제15권7호
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• pp.3253-3260
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• 2014

Background: A case-control study was conducted to evaluate the effect of household exposure, dietary habits, smoking and Glutathione S-Transferases M1, T1 polymorphisms on lung cancer among women in Mizoram, India. Materials and Methods: We selected 230 newly diagnosed primary lung cases and 460 controls from women in Mizoram. Multivariate logistic regression analysis was performed to estimate adjusted odds ratio (OR). Results: Exposure of cooking oil fumes (p<0.003), wood as heating source for cooking (p=0.004), kitchen inside living room (p=0.001), improper ventilated house (p=0.003), roasting of soda in kitchen (p=0.001), current smokers of tobacco (p=0.043), intake of smoked fish (p=0.006), smoked meat (p=0.001), Soda (p<0.001) and GSTM1 null genotype (p=0.003) were significantly associated with increased risk of lung cancer among women in Mizoram. Significantly protective effect was observed for intake of bamboo shoots (p=<0.001) and egg (p<0.001). A clear increase in dose response gradient was observed for total cooking dish years. Risk for lung cancer tends to increase with collegial effect of indoor environmental sources (p=0.022). Significant correlation was also observed for interaction of GST polymorphisms with some of dietary habits. Conclusions: We confirmed the important role of exposure of cooking oil emission and wood smoke, intake of smoked meat, smoked fish and soda (an alkali preparation used as food additives in Mizoram) and tobacco consumption for increase risk of lung cancer among Women in Mizoram.