• The Journal of Korean Academy of Prosthodontics
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• v.35 no.4
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• pp.647-661
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• 1997

The resin modified glass ionomer cements(RMGICs) have been used for years since 1989. Recently it has been developed for luting of fixed restorations. To evaluate the bond strength and marginal leakage of RMGICs for luting usage, the 80 extracted human molars which had uniform area of exposed dentin were cemented with 3 types of RMGICs(Fuji Duet, Advance, Vitremer), a conventional Glass Ionomer Cement(GIC-Fuji I), and a resin cement (Panavia 21) to base metal alloy(Ni-Cr-Be). After thermocycling the specimens were immersed in basic fuschin dye for measuring marginal leakage. The shear bond strength was measured with Instron and the maximum dye penetration was measured to 0.1mm. The types of fractured patterns were determined with stereoscope(${\times}7.5$). The results are as follows. 1. The difference between 3 RMGICs and a conventional GIC in shear bond strength was not statistically significant. It seemed that RMGICs had lower shear bond strength than resin cement. (p<0.01) 2. The mean scores of marginal leakage had no significant difference between the resin cement and 3 RMGICs but it was much higher in conventional GIC than the RMGICs (p<0.05) 3. It was determined that the manufacturer and the methods of dentin pretreatment determined the pattern of fracture surfaces and the frequency of adhesive failure between teeth and 3 RMGICs standed as in following order - Vitremer, Advance, Fuji Duet. (p<0. 01) When the fracture pattern was analyzed, it could be said that the materials and the method of dentin pretreatment have much effect on bonding states. This means that the dentin bonding agents should be improved. But the limited products in this experiment can not evaluate the physical properties of the entire RMGICs. Therefore a further study which can evaluate various RMGICs should be in progress to develope better cements.

• Journal of Adhesion and Interface
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• v.2 no.3
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• pp.9-15
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• 2001

To estimate the effect of the surface state of the EVA foam on an adhesion, the surface was treated with solvents having different values of a solubility parameter and a surface tension. The morphology and the contact angle were measured by SEM and contact angle tester, respectively. The value of the critical surface tension(${\gamma}_{c,0}$) of the EVA foam calculated by Zismann plot was 27.08 dyne/cm. The surface state and the ${\gamma}_c$ of the EVA foam were changed by solvent treatment. The swelling ratio of EVA foam was influenced by solubility parameter of a solvent, on the other hand the degree of change in the surface state was dependent on a surface tension of a solvent. When a surface tension of solvent. When a surface tension of solvent is lower than ${\gamma}_{c,0}$ of the EVA foam, the wettability of a solvent was good and the surface state was greatly changed. The adhesion strength of EVA foam was greatly improved to above 600% as compared to that of the untreated one. As the ${\gamma}_c$ of EVA was closed to the surface tension of the primer, the good adhesion strength was obtained.

• Analytical Science and Technology
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• v.9 no.3
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• pp.279-291
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• 1996

The new chelating resins, XAD-2, 4, 16-TAC and XAD-2, 4, 16-TAO were synthesized by Amberlite XAD-2, XAD-4, and XAD-16 macroreticular resins with 2-(2-thiazolylazo)-p-cresol(TAC) and 4-(2-thiazolylazo)orcinol(TAO) as functional groups and were characterized by elemental analysis and FT-IR spectrometry. It was found that the content of functional group in chelating resin was 0.60mmol/g in XAD-16-TAC and 0.68mmol/g in XAD-16-TAO respectively. The chelating resins were stable in acidic and alkaline solution and can be reused over 10 times. The sorption behavior of some metalions to two chelating resins was investigated by batch method, which included batch equilibrium, effect of pH, coexisting ions and masking agent. For the optimum condition of sorption, the time required for equilibrium was about 1 hour and optimum pH was 5. In the presence of anions such as ${SO_4}^{2-}$ and $CH_3COO^-$, the sorption of U(VI) ion was slightly reduced but other anions such as $Cl^-$ and $NO{_3}^-$ revealed no interference effect. Also, sorption capacity of U(VI) ion was decreased by addition of $CO{_3}^{2-}$ ion because of complex formation of $[UO_2(CO_3)_3]^{4-}$, but alkali metals and alkali earth metals including Na(I), K(I), Mg(II), and Ca(II) were not affected for the sorption extent. Masking agent, NTA showed better separation efficiency of U(VI) ion from coexisting metal ions such as Th(IV), Zr(IV), Hf(IV), Cu(II), Cd(II), Pb(II), Ni(II), Zn(II) and Mn(II) than EDTA, CDTA.

• Korean Journal of Microbiology
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• v.39 no.3
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• pp.166-174
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• 2003

Microcystin produced by cyanobacteria in surface waters, such as eutrophic lake and river, is a kind of serious environmental problems due to its toxicity to human and wild animals. Microcystin is synthesized nonribosomally by the large modular multi-functional enzyme complex known as microcystin synthetase encoded by the mcy gene cluster. Amplification of mcy genes by PCR from cultures and environmental samples is a simple and efficient method to detect the toxigenic Microcystis. In order to evaluate primers designed to detect toxic microcystin-producing strains, 17 cyanobacterial strains and 20 environmental samples were examined by PCR with 7 pairs of primers. Some microcystin-producing cyanobacteria were not detected with FAA-RAA, TOX4F-TOX4R and FP-RP primers. The fragment of unexpected size was amplified with NSZW2-NSZW1 primers in Microcystis strains isolated from the lakes in Korea. TOX1P-TOX1F primers failed in amplification of toxin-producing strains. Only MSF-MSR and TOX2P- TOX2F primers amplified the fragments of mcy genes from 11 strains of microcystin-producing Microcystis. The water samples taken from 20 lakes in Korea were analyzed by PCR using each of the primers. In all the water samples, cyanobacteria capable of producing microcystin were detected by the PCR with TOX2P-TOX2F primers. These results indicate that TOX2P-TOX2F primers are better than the other primers for detection of microcystin-producing Microcystis strains in Korea. The nucleotide sequences of mcy gene in Microcystis aeruginosa NIER10010 suggest genetic diversity of Korean isolates.

• The Journal of the Petrological Society of Korea
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• v.20 no.3
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• pp.161-172
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• 2011

Open beaker digestion method is routinely used as the sample preparation technique for trace element determination of rock samples by inductively coupled plasma mass spectrometry, With this method, however, dissolution of Zr and Hf is not always guaranteed especially when the samples contain refractory minerals. In this study, glass bead digestion technique was compared with conventional open beaker digestion technique for the sample preparation of three USGS rock standards such as AGV-2, BHVO-2, and G-3. Thirty trace elements including rare earth elements were analysed by ICP-MS and ICP-AES. There were no clear differences in analytical results for the AGV-2 and BHVO-2 standards between the two techniques, but Zr, Hf, Y, and middle- to heavy- rare earth element concentrations of the G-3 standard prepared by open beaker digestion technique were significantly lower than the recommended values. This can be attributed to the presence of refractory mineral zircon. On the contrary, all the analytical results of the G-3 standard prepared by glass bead digestion technique were in good agreement with the recommended values, indicating complete dissolution of zircon. The analytical results show that the volatile elements such as Pb and Zn were not lost during the preparation of glass bead. Low dilution glass bead digestion technique described here will be very helpful to enhance precision and accuracy of trace element analysis for geological samples containing refractory minerals.

• Korean Journal of Soil Science and Fertilizer
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• v.34 no.1
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• pp.1-7
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• 2001

Total upland area for cultivating the vegetable crops in the Alpine soils of Northeastern South Korea has been extending its limit to meet the increasing demand of vegetable food in recent decades. About 70% of these alpine soils are located in over 7% of the slope and most of vegetable crops have been cultivated intensively without practicing the best management systems. Thus, soil erosion and continuous cropping system have degenerated the soil fertility and shown detrimental effects on water quality. We initiated an intensive and extensive investigation to characterize the fertility problems encountered in these uplands. Objectives of this paper were to characterize the fertility status in the Alpine soils cultivated with vegetable crops for many years and to provide the recommendations for adequate soil management measures including fertilization and erosion control. Soils in general have good drainage with textural classes of loam or sandy loam. Their topographical characteristics tended to lead them to shallow plow layers, and the steepness of the terrain created erosion hazard. Of the soils examined, about 11% of uplands over 30% gradient was found in need of an urgent reforestation. A high content of gravel and firm hardness of soil attributed to inhibit the utilization of farm machinery and plant-root development. The average soil pH 5.6 was slightly low relative to pH 5.70 of the national average. Organic matter content was high compared with 2.0% of national average, but decreased with the prolonged cultivation periods. Available $P_2O_5$ concentration was unusually high due to the consequence of over dose application with chemical and organic fertilizers. Exchangeable cations as Ca, Mg, and K were appeared to be decreased in these regions with prolonging the cultivation periods. There were no significant differences in cation exchange capacity (CEC) and electrical conductivity (EC) among locations. Heavy metal contents were mostly lower than the threshold of danger level designated by Soil Environment Conservation Law of South Korea. Results indicated that a proper countermeasure and the best management practice should be immediately implemented to conserve the top soil and fertility in the Alpine regions.

• Economic and Environmental Geology
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• v.42 no.2
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• pp.121-131
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• 2009

The distribution characteristics of heavy metals for surface sediments in east oceanic dumping area (EDA) and west oceanic dumping area (WDA) are evaluated by grain sizes, minerals, sedimentation rates and compositions of heavy metals. The mean grain sizes in EDA and WDA range from $7.95{\Phi}$ to $8.51{\Phi}$ and $7.42{\Phi}$ to $8.15{\Phi}$, respectively. These are mostly belonging to the M (mud) type. Minerals in the surface sediments consist of illite with chlorite, smectite, and kaolinite. Sedimentation rates estimated by $^{210}Pb$ method in EDA and WDA are 1.11 mm/yr$\sim$1.73 mm/yr and 1.87 mm/yr, respectively. According to the interrelationship, concentrations of Ni, Cu, Cr, and Zn are closely associated with mean grain size, Al, and Fe, whereas concentrations of Cd and Pb are poorly associated with ones. The enrichment factors of these elements are higher than 1.5, suggesting that the concentrations of Cd and Pb in the surface sediments are affected by anthropogenic sources. The $I_{geo}$-class numbers of Cd and Pb in the surface sediments are mostly classified in 2 to 4, showing moderate to strongly polluted. These numbers in EDA are higher than that of WDA, and the highest number is 4, indicative of the strongly polluted class. Our results show that the disposed wastes at EDA include mineralogical wastes, dredged materials from sewage disposals, and sludges from constructions having materials of WDA. The annual amount of oceanic dumping in EDA is double than that in WDA.

• Korean Journal of Heritage: History & Science
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• v.44 no.2
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• pp.4-25
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• 2011

This study was performed in 2010 to examine the flora and vegetation structure and chemical characteristics of soil in the growing community of Abeliophyllum distichum, located in the Byeonsanbando National Park. This Abeliophyllum distichum community has more individual numbers in Cheongrim-ni and Jungkye-ri, Byeonsan-myeon, and Buan-gun area, which is designated as a Natural Monument (No. 370), and also where the habitat conditions for Abeliophyllum distichum is more favorable. The authors recorded 100 taxa with 45 families, 82 genus, 93 species, 4 varieties, and 3 forms. Among them, species such as Abeliophyllum distichum (critically endangered), Asarum maculatum (near threatened) and Chionanthus retusa (near threatened), which are categorized as rare plants, were recorded. According to the list of Korean endemic plants, 4 taxa, particularly Philadelphus schrenckii, Abeliophyllum distichum, Weigela subsessilis, and Lonicera subsessili, were recorded. The community of Abeliophyllum distichum is located in the northwest slope of Baekcheon watershed and the community is comprised of healthy soil. The community structure was classified into three: the Castanea crenata community, Zelkova serrata community, and Quercus serrata community. The Castanea crenata community is composed of the Cornus walteri, Platycarya strobilacea, Zelkova serrata, Rhamnella frangulioides, arranged in terms of importance percentage. The Zelkova serrata community is composed of Celtis sinensis, Quercus aliena, Styrax japonica, and Acer pseudo-sieboldianum, also according to importance percentage. As for the Quercus serrata community, it is composed of Quercus variabilis, Castanea crenata, and Prunus sargentii, also arranged in terms of importance percentage. The importance percentage of Abeliophyllum distichum is 6.6% in the Castanea crenata community, 5.6% in the Zelkova serrata community and 5.1% in the Quercus serrata community. Moreover, in order of chemical characteristics of soil pH, electrical conductivity, available phosphoric, organic matter, and exchangeable cation (K, Ca, Mg) are analyzed. The No. 3 site was relatively higher than other districts of the same chemical characteristics of soil.

• Microbiology and Biotechnology Letters
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• v.44 no.3
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• pp.355-362
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• 2016

The phosphomannomutase/phosphoglucomutase gene (pmm/pgm) of Vibrio anguillarum (the causative agent of fish vibriosis) was cloned, and the open reading frame corresponded to a protein with 446 amino acids. The pmm/pgm gene showed a significant degree of sequence homology with the previously reported genes from V. mimicus, V. vulnificus, V. splendidus, and V. harveyi, with 92.3%, 91.4%, 89.9%, and 89.9% amino acid identity, respectively. By reverse transcriptase-polymerase chain reaction, we found that the pmm/pgm gene was upregulated under cold stress condition. The PMM/PGM protein is known to catalyze the interconversion between mannose-1-phosphate and mannose-6-phosphate or glucose-1-phosphate and glucose-6-phosphate, which are important intermediates for lipopolysaccharide (LPS) biosynthesis. To confirm the role of PMM/PGM in the LPS biosynthetic pathway, we constructed a knock out mutant by homologous recombination. The respective LPSs were isolated from the V. anguillarum wild-type and mutant strains, and changes were compared by subjecting them to sodium dodecyl sulfate polyacrylamide gel electrophoresis. Based on the different patterns of the LPSs, we expect the pmm/pgm gene to have an important role in LPS biosynthesis. The pmm/pgm-deficient mutant of V. anguillarum will contribute to further studies about the role of LPS in V. anguillarum pathogenesis.