• Title/Summary/Keyword: $Na^+\-Ca^{2+}$ exchange

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Effects of $Cd^{2+}$ on the Contractility in the Antral Circular Muscle of Guinea-pig Stomach

  • Kim, Eui-Yong;Han, Jin
    • The Korean Journal of Physiology
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    • v.26 no.2
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    • pp.129-136
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    • 1992
  • The effects of $Cd^{2+}$ on spontaneous contraction, and the contractures induced by $0mM\;Na^+,\;60mM\;K^+\;and\;10^{-6}\;M$ acetylcholine, 1mM caffeine were studied in order to elucidate diverse actions of $Cd^{2+}$ on the $Ca^{2+}$ mobilization related with contractility in the antral circular muscle of guinea pig stomach. $Cd^{2+}$ inhibited the spontaneous contraction in a does dependent manner $(10^{-6}\;M\;10^{-4}\;M).\;Cd^{2+}\;(3{\times}10^{-5}M)$ suppressed 60 mM $K^+$ induced contracture composed or a phasic and a tonic response and the increased tonic response by the increased external $Ca^{2+}$ concentration. $Cd^{2+}$ also suppressed acetylcholine induced contracture composed of repetitive phasic and a tonic component and the increased tonic response by the increased external $Ca^{2+}$ concentration. Caffeine in the concentration of 1mM evoked contracture but $Cd^{2+}$ suppressed the contracture. $Cd^{2+}$ suppressed the amplitude of the $Na^+$ tee contracture dose dependently and the amplitude of $Na^+$ free contracture almost decreased to 20% of control amplitude in the concentration of $10^{-4}\;M\;Cd^{2+}$. From the above results, it is suggested that $Cd^{2+}$ may inhibit not only $Ca^{2+}$ influx via voltage sensitive, receptor operated $Ca^{2+}$ channel and Na/ca exchange but also intracellular $Ca^{2+}$ release from the sarcoplasmic reticulum in the antral circular muscle of guinea pig stomach.

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Electrical conductivity in oxide glasses subjected to $sodium \leftrightarrow silver$ ion exchange treatment (산화물유리에서의 $Na^+$이온과 $Ag^+$이온 교환에 따른 전기전도도 변화)

  • 한준수;강원호;이효경
    • Electrical & Electronic Materials
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    • v.9 no.3
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    • pp.284-290
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    • 1996
  • The electrical properties of bulk galsses in the system Na$_{2}$O-CaO-Al$_{2}$O$_{3}$-B$_{2}$O$_{3}$-SiO$_{2}$ containing 20 to 30mol% sodium which have been subjected to a sodium .tautm. silver ion exchange reaction for 24, 36 and 48 hrs. were analysed by impedance spectroscopy method. Ion exchanged glasses exhibit activation energy values lower than those of the untreated ones. The electrical conductivity increase with sodium content and ion exchanging time. In this experiment the electrical conductivity exhibits a manximum value of 1.78*10$^{-4}$ S/cm at 200.deg. C which contains 30mol% sodium and subjects ion exchange reaction for 48hrs.

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Influence of the Starting Materials and Sintering Conditions on Composition of a Macroporous Adsorbent as Permeable Reactive Barrier (초기 소재와 소성조건이 투수반응벽체인 대공극흡착제 조상에 미치는 영향)

  • Chung, Doug-Young;Lee, Bong-Han;Jung, Jae-H.
    • Korean Journal of Soil Science and Fertilizer
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    • v.42 no.4
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    • pp.239-248
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    • 2009
  • In this investigation, we observed surface morphology and porosity of a macroporous adsorbent made of Na-bentonite and Ca-bentonite as structure formation materials and grounded waste paper as macropore forming material for the development of a permeable reactive barrier to remove heavy metals in groundwater. Therefore, we selected minerals having higher cation exchange capacity among 2:1 clay minerals and other industrial minerals because sintering can significantly influence cation exchange capacity, resulting in drastic decrease in removal of heavy metals. The results showed that the increasing sintering temperature drastically decreased CEC by less than 10 % of the indigenous CEC carried by the selected minerals. One axial compressibility test results showed that the highest value was obtained from 5% newspaper waste pulp for both structure formation materials of Na-bentonite and Ca-bentonite although there were not much difference in bulk density among treatments. The pore formation influenced by sintering temperature and period contributes removal of heavy metals passing through the sintered macroporous media having different water retention capacity.

Effect of Cations on Resting Potential and Pump Activity of Unfertilized Mouse Eggs (Mouse Egg의 안정막전압과 Pump 활동에 대한 양이온의 효과)

  • Hong, Seong-Geun
    • The Korean Journal of Physiology
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    • v.23 no.1
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    • pp.35-42
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    • 1989
  • The present study was performed to observe the effects of cations on resting membrane potential and pump activity in the unfertilized eggs of ICR strain mice. After an induction of superovulation, the fresh eggs with zona pellucida were collected and the membrane potentials were recorded. Recordings of membrane potential in this study was obtained from the physiological conditions ($37^{\circ}C$ and 4mM Ca in standard solution), differently from the another reports with unphysiological conditions (room temprature and high Ca in standard solution) for a stable and long-lasting observations. Presented data was obtained within 6 hours after collection from the oviduct. The results observed are as follows, 1) Resting potential of the unfertilized eggs was $-25.8{\pm}3.8mV$ $(Mean{\pm}Se,\;n=31)$. 2) As the K ion concentration was increased, resting membrane potential was depolarized but showed hyperpolarization with $K^{+}$ below 25mM. 3) Alteration of the resting membrane potential for the changes of $Na^{+}$ concentration were hardly observed, while resting potential was hyperpolarized as $Ca^{2+}$ concentration was increased. 4) Pump activity as transient or prolonged hyperpolarization was $-2.29{\pm}0.75mV$ $(Mean{\pm}Se,\;n=16)$, the hyperpolarization was increased in both amplitude and duration under the 10mM $Ca^{2+}$ solution. 5) Hyperpolarization due to pump activity was decreased or disappeared by $5{\times}10^{-5}\;M$ ouabain treatment and could not be observed under the both Na-free and Ca-free solutions. 6) Above results are likely to suggest that the resting potential of the mouse unfertilized eggs is affected to mainly by Ca-dependent K conductance and Na-Ca exchange mechanism and that there is pump activity coupling between $K{+}$, $Na^{+}$ and $Ca^{2+}$.

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Measuring of $Na^+-Ca^{2+}$ Exchange Current in Cerebellar Purkinje Cells

  • Cho, Chung-Hyun;Daejong Jeon;Shin, Hee-Sup;Lee, Chin O.
    • Proceedings of the Korean Biophysical Society Conference
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    • 2002.06b
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    • pp.37-37
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    • 2002
  • The Na$^{+}$-Ca$^{2+}$ exchanger (NCX) is known to playa critical role in the regulation of intracellular $Ca^{2+}$ in many tissues and cells. Three isoforms have been cloned (NCXl, NCX2, NCX3). Among the isoforms, NCX2 and NCX3 are expressed at high levels in brain and in a few other tissues. But the differential properties of the isoforms are not yet clearly established.(omitted)

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A review on the softening of the fermented vegetables and the fruits (침채류와 과실의 연화현상에 관한 고찰)

  • 이희섭
    • Journal of the Korean Home Economics Association
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    • v.34 no.4
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    • pp.403-414
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    • 1996
  • The softening of the femented vegetables and the fruits was resulted from the degradation of pectin substances, cellulose, hemicellulose by polygalacturonase(PG), pectinesterase(PE), Cx-cellulase, $\beta$-galctosidase. The conversion of insoluble pectin to soluble pectin in cell wall-middle lamella was a major factor in the changes of firmness. Ca2+ was substantially increased firmness. However, Ca2+ could be removed from cell wall by chelating agents such as oxalic acid and citric acid. And Ca2+ was replaced with Na+ by ion exchange reaction. Ca2+ deficient tissue was vulnerable to attack by PG. Preheating treatment and Ca2+ addition is most effective in inhibiting the vegetable food softening and in increasing middle lamella-cell wall regidity, which PE activation by preheating treatment and Ca2+ addition could created more anionic carboxyl groups for cationic materials binding such as Ca2+ and chitosan and for polypectategel formation. Excessive demethylation by PE was associated with loosening of middle lamella cell wall components and softening.

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Effect of $Ca^{++}$ on High K-induced Contracture of Isolated Frog Ventricular Muscle (적출 심근의 칼륨경축에 대한 칼슘이온 효과)

  • Choi, Youn-Baik;Kim, Ki-Whan
    • The Korean Journal of Physiology
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    • v.20 no.1
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    • pp.31-41
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    • 1986
  • The sufficient myoplasmic $Ca^{++}$ to react with the contractile proteins is necessary to induce contraction of a cardiac muscle. These $Ca^{++}$ for the production of muscle contraction are supplied from the three recognized $Ca^{++}$ sources; internal $Ca^{++}$ release via the sarcoplasmic reticulum(SR), $Ca^{++}$ influx through a gated Ca-channel in the membrane as a Isi, and $Ca^{++}$ transport by the mechanism of Na/ca exchange. However, it is still controversial which $Ca^{++}$ sources act as a main contributor for myoplasmic $Ca^{++}$, Therefore, this study was undertaken in order to examine the $Ca^{++}$ sources for the contraction of frog ventricle. There is evidence that the SR is sparse in frog ventricular fibers, and that T-tubules are absent. Isolated ventricular strips of frog, Rana nigromaculata, were used in this experiment. Isometric tension was recorded by force transducer, and membrane potentials of ventricular muscles were measured through the intracellular glass microelectrodes, which were filled with 3M KCI and had resistance of $30{\pm}50M{\Omega}$. All experiments were performed at room temperature in a tris·buffered Ringer solution which was aerated with 100% $O_2$. Isotonic high K, low Na solution was used to induce K-contracture, K-contracture appeared at the concentration of 20 to 30mM-KCI and was potentiated in parallel with the increase in KCI concentration. The contracture had two components: an initial rapid phasic and a subsequent slow tonic contractile responses. Membrane Potentials measured at normal Ringer solution(2.5mM KCI) was -90 to -100 mV, and decreased linearly as the KCI concentration increased; -55mV at 20mM.KCI, -45mV at 30 mM.KCI, -30 mY at 50 mM.KCI, and -12 mV at 100 mM.KCI. K-contracture was evoked firstly at the membrane potential of -45 mV. The contracture was potentiated by the increase of bathing extracellular $Ca^{++}$ concentration. However, in the absence of $Ca^{++}$ the contracture was almost not induced by 50 mM.KCI solution. Caffeine(20mM) in normal Ringer solution, which is known to release $Ca^{++}$ from SR without substantial effects on the $Ca^{++}$ fluxes across the surface membrane, did not affect membrane potential and also not initiate contracture, but the caffeine in 20 mM-KCI Ringer solution produced a contracture. Above results suggest that the main $Ca^{++}$ source for the K·contracture of frog ventricle is $Ca^{++}$ influx through the voltage-dependent Ca-channel, and that in the K-contracture at the concentration of 100 mM-KCI, the mechanism of Na/ca exchange also partly contributs, in addition to the $Ca^{++}$ influx.

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Comparison of Soil Ion, Plant Nutrient Contents and Growth in Quercus mongolica Forests in Seoul and Its Vicinity (수도권 일대 신갈나무 숲의 토양이온, 식물체 영양염류 함량 및 생장의 비교)

  • 김준호;임병선;김종욱
    • The Korean Journal of Ecology
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    • v.22 no.1
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    • pp.13-19
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    • 1999
  • To investigate and compare the effects of air pollution near Seoul on soil ion, plant nutrient contents and plant growth, 14 Quercus mongolica forests were studied from Mt. Namsan in Seoul to Mt. Maebongsan in Kangwon-Do along the line transect. Soil pH values decreased as approached to Seoul. Concentrations of basic cations such as exchangeable Ca/sup 2+/, Mg/sup 2+/ and Na/sup +/ and effective cation exchange capacity(ECEC) in forest soil decreased as approached to Seoul. Ca/sup 2+/ and Mg/sup 2+/ contents in l-year-old Q. mongolica twigs decreased, but their K/sup +/ and Na/sup +/ contents increased as approached to Seoul. Ca/sup 2+/ contents in l-year-old Q. mongolica leaves decreased, but their K/sup +/ and Mg/sup 2+/ contents increased as approached to Seoul. Length of l-year-old twigs generally decreased near Seoul. Tree-ring analyses of Q. mongolica trees in Mt. Namsan and Mt. Yebongsan showed that mean tree-ring width in Mt. Namsan was narrower than that of Mt. Yebongsan from early 1970's to late 1980's.

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Evaluation of Groundwater Quality in Crystalline Bedrock Site for Disposal of Radioactive Waste (방사성폐기물 처분을 위한 결정질 기반암의 지하수 수질 평가)

  • Lee, Jeong-Hwan;Jung, Haeryong;Cheong, Jae-Yeol;Park, Joo-Wan;Yun, Si-Tae
    • Journal of Nuclear Fuel Cycle and Waste Technology(JNFCWT)
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    • v.12 no.4
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    • pp.275-286
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    • 2014
  • This study evaluated the evolution stage and origin of chemical components of 12 boreholes at crystalline bedrock using multivariate statistical and groundwater quality analyses. Groundwater types are mostly belonged to Na(Ca)-$HCO_3$ and Ca-$HCO_3$ types, indicating that directly reaction of cation exchange ($Ca^{2+}{\rightarrow}Na^+$) prevailed. The degree of groundwater evolution is included the range from low to intermediate stage based on field and laboratory analytical conditions. As a result of multivariate statistical analysis, a typical indicator of groundwater contamination, $NO_3$-, has the positive correlation with $Na^+$ and $Cl^-$. The origin of sea spary ($Cl^-$) has the positive correlation with $Na^+$, $SO{_4}^{2-}$, $Mg^{2+}$, and $K^+$, while not correlation with $Ca^{2+}$, $Fe^{2+}$, $HCO_3{^-}$, $F^-$, and $SiO_2$. The concentration of $Cl^-$ and $NO_3{^-}$ belongs to general quality of groundwater and not exceeds over the Korean standard for drinking water. And the negative values of saturation index of minerals are calculated with chemical components in groundwater. Therefore, most of chemical components of groundwater in the study area are originated from natural process between rock and groundwater, whereas some of components are derived from sea spary and anthropogenic sources related to agricultural activities.

Mineral Chemistry and Thermo-chemical Characterization of Wellsite, a Barrian Zeolite, from the Tertiary Formation in Gampo Area (감포 지역의 제3기 층에서 산출되는 Ba-제올라이트인 웰자이트의 광물화학 및 열화학적 특성)

  • 노진환;김기업
    • Journal of the Mineralogical Society of Korea
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    • v.10 no.2
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    • pp.105-113
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    • 1997
  • Mineral description and mineralogical characterization were made for the wellsite, a barrian zeolite, which found as diagenetic alterations in the Miocene pyroclastic rocks in Gampo area. The wellsite occurs together with clinoptilolite, smectite and apatite as euhedral crystallites (0.2~0.4mm) forming interpenetraion twinning in the vesicles of altered pmice fragments. Compared to other reported wellsites, the wellsite is rather silicic (Si/(Al+Fe): 3.12-3.16) and Ca-rich. Unit cell dimensions and chemical formular determined from XRD, EMPA and TGA data are as follows:a=9.883$\AA$, b=14.204$\AA$, c=8.677$\AA$, $\beta$-124.764$^{\circ}$, (Ba0.57K0.36)(Ca1.18Na0.04)Al3.9Si12.1O32.13.9H2O.The cation composition of the Gampo wellsite, which shows an exchange reaction in the form of Ba2++Ca2+=2(K++Na-), is deviated far from the compositional range of a phillipsite-harmotome series. Due to higher abundance of divalent cations (Ca, Ba) and si in the wellsite, cimpared to those of the phillipsite and harmotome reported in other areas, the zeolite seems to be characteristic of higher water content (18.7 wt%) and higher thermal stability. XRD, chemical and thermo-chemical results of the wellsite reflects that wellsite is rather a Ba- and Ca-rich end member of a phillipsite-harmotome-wellsite series than an intermediate phase of phillipsite-harmotome series or a barrian variety of phillipste.

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