• Title/Summary/Keyword: $M_3C$

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$H_{2}$ production of photosynthetic bacteria transferred TOL plasmid from flavobacterium odoratum (Flavobacterium odoratum의 TOL 플라스미드를 전달받은 광합성세균으로부터의 수소 생성)

  • 오순옥;조인성;이희경;민경희
    • Korean Journal of Microbiology
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    • v.29 no.6
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    • pp.408-415
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    • 1991
  • TOL plsmid size of Flavobacterium odoratum SUB53 was estimated as 83 Md and the optimum concentration of m-toluate degradation by TOL plasmid was 5 mM. $H_{2}$ production by Rhodopseudomonas sphaeroides KCTC1425 was largely dependent on nitrogenase activity and showed the highest at 30 mM malate with 7 mM glutamate as nitrogen source. Nitrogenase activities were inhibited by 0.3 mM $NH_{4}^{+}$ions, to be appeared the decrease of $H_{2}$ production. Conjugation of TOL plasmids from F. odoratum SUB53 and Pseudomonas putida mt-2 to R. sphaeroides showed the optimum at the exponential stage of recipient cells in presence of helper plasmid pRK2013. According to the investigation of catechol-1,2-oxygenase (C-1, 2-O) and catechol-2,3-oxygenase (C-2,3-O) activities of R. sphaeroides C1 (TOL SUB53) and C2 (TOL mt-2), the gene for C-2,3-O is located on TOL plasmid and gene for C-1, 2-O on the chromosome of R. sphaeroides. m-Toluate was biodegraded by TOL plasmid in R. sphaeroides C1 and C2, presumably to be produced $H_{2}$ gas from the secondary metabolites of m-toluate.e.

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Antioxidative Activities and Inhibitory Effects on Lipid Accumulation of Extracts from Different Parts of Morus alba and Cudrania tricuspidata (뽕나무(Morus alba)와 꾸지뽕나무(Cudrania tricuspidata)의 부위에 따른 항산화 활성 및 3T3-L1세포 지방축적 억제 효과)

  • Kim, Gun-Hee;Kim, Eunhyang
    • The Korean Journal of Food And Nutrition
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    • v.32 no.2
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    • pp.138-147
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    • 2019
  • In this study, we examined antioxidative effects and the anti-adipogenesis effect of different parts of Cudrania tricuspidata (C), and Morus alba (M). Total polyphenol contents were highest in M-root ($34.56{\pm}0.045mg\;GAE/g$), and there was no significant difference, between C-root and M-leaf. Total flavonoid contents of C-root were highest ($23.07{\pm}0.004mg\;QE/g$). To examine antioxidant activities of C and M extracts, DPPH and ABTS radical scavenging activity, and FRAP assay, was used. Results show that antioxidant activities of C and M extracts increased, in a dose-dependent manner. Adipocytes are generated by preadipocyte differentiation, during adipogenesis. Matured adipocytes accumulate in abnormal and cause obesity. We investigated effects of leaf and root extracts of C and M, on lipid accumulation, in 3T3-L1 adipocytes. Changes in cell morphology, and degrees of lipid accumulation in adipocytes, were evaluated by Oil Red O staining. Root extracts of C and M, reduced lipid content in a dose-dependent manner. Therefore, root extracts of C and M, may be good candidates for managing obesity.

R-Curve Behavior and Mechanical Properties of Al2O3 Composites Containing SiC and TiC Particles (SiC와 TiC 입자를 함유하는 Al2O3 입자복합체의 균열저항거동과 기계적 성질)

  • Na, Sang-Woong;Lee, Jae-Hyung
    • Journal of the Korean Ceramic Society
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    • v.39 no.4
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    • pp.413-419
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    • 2002
  • Particulate composites of $Al_2O_3$/TiC/SiC, $Al_2O_3$/TiC and $Al_2O_3$/SiC have been fabricated by hot pressing and their R-curve behaviors and mechanical properties were investigated. $Al_2O_3$ containing 30 vol% TiC particles showed higher toughness by 8% than that for monolithic alumina and its fracture strength was increased significantly by approximately 30%. On the other hand, the addition of 30 vol% SiC of $3{\mu}m$ in $Al_2O_3$ decreased the fracture strength slightly but induced a rising R-curve behavior owing to the strong crack bridging of SiC particles. In case of $Al_2O_3$/TiC/SiC, arising R-curve behavior was also observed and the fracture toughness reached 6.6 MPa${\cdot}\sqrt{m}$ at the crack length of $1000{\mu}m$, which was lower than that of $Al_2O_3$/SiC, however, while the fracture strength was higher by about 20%. The fracture toughness seemed to be decreased as smaller TiC particles roughened the SiC interface and pullout of the SiC particles for crack bridging became less active.

A study on polycrystalline 3C-SiC etching with magnetron applied reactive ion etching for M/NEMS applications (마그네트론 RIE을 이용한 M/NEMS용 다결정 3C-SiC 식각 연구)

  • Chung, Gwiy-Sang;Ohn, Chang-Min;Nam, Chang-Woo
    • Journal of Sensor Science and Technology
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    • v.16 no.3
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    • pp.197-201
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    • 2007
  • The magnetron reactive ion etching (RIE) characteristics of polycrystalline (poly) 3C-SiC grown on $SiO_{2}$/Si substrate by APCVD were investigated. Poly 3C-SiC was etched by $CHF_{3}$ gas, which can form a polymer as a function of side wall protective layers, with additive $O_{2}$ and Ar gases. Especially, it was performed in magnetron RIE, which can etch SiC at a lower ion energy than a commercial RIE system. Stable etching was achieved at 70 W and the poly 3C-SiC was undamaged. The etch rate could be controlled from $20\;{\AA}/min$ to $400\;{\AA}/min$ by the manipulation of gas flow rates, chamber pressure, RF power, and electrode gap. The best vertical structure was improved by the addition of 40 % $O_{2}$ and 16 % Ar with the $CHF_{3}$ reactive gas. Therefore, poly 3C-SiC etched by magnetron RIE can expect to be applied to M/NEMS applications.

Characteristics of polycrystalline 3C-SiC micro resonator (다결정 3C-SiC 마이크로 공진기의 특성)

  • Lee, Tae-Won;Chung, Gwiy-Sang
    • Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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    • 2008.06a
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    • pp.69-70
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    • 2008
  • Micro resonators have been actively investigated for bio/chemical sensors and RF M/NEMS devices. Among various materials, SiC is a very promising material for micro/nano resonators since the ratio of its Young's modulus, E, to mass density, $\rho$, is significantly higher than other semiconductor materials, such as, Si and GaAs. Polycrystalline 3C-SiC cantilever with different lengths were fabricated using a surface micromachining technique. Polycrystalline 3C-SiC micro resonators were actuated by piezoelectric element and its fundamental resonance was measured by a laser vibrometer in air and vacuum at room temperature, respectively. For the cantilever with $100{\mu}m$ length, $10{\mu}m$width and $1.3{\mu}m$ thickness, the fundamental frequency appeared at 147.2 kHz.

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Disposal Possibility of Raw Food Wastes Using Earthworm, Eisenia andrei (지렁이를 이용한 생 음식물쓰레기 체리 가능성)

  • Na Young Eun;Nam Hong Shik;Han Min Su;Bang Hae Sun;So Kyu Ho;Bae Yun Hwan;Ahn Yong Joon
    • The Korean Journal of Soil Zoology
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    • v.8 no.1_2
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    • pp.13-16
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    • 2003
  • Disposal possibility of raw food wastes which have various characters without any washing and composting process was tested directly using the earthworm, Eisenia andrei. The amount of feeding treatment a day by the earthworm was investigated according to input amount of 1.5 kg/m$^2$ or 3.0 kg/m$^2$ of fifteen food wastes with the different pH and EC. Earthworm disposed an average of 0.87 kg/m$^2$/day of food wastes at the constant temperature of 15$^{\circ}C$ and 1.01 kg/m$^2$/day at 2$0^{\circ}C$. The most disposal among fifteen food wastes was the food waste with pH 5.3 and EC 17.7 as 1.59 kg/m$^2$/day at 15$^{\circ}C$ and as 1.63 kg/m$^2$/day at 2$0^{\circ}C$. The least disposal was the food waste with pH 3.9 and EC 17.7 as 0.31 kg/m$^2$/day at 15$^{\circ}C$ and as 0.53 kg/m$^2$/day at 2$0^{\circ}C$. It took an average 4 days to dispose the amount of 3 kg raw food wastes at 15$^{\circ}C$ and 3.3 days at 2$0^{\circ}C$.

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A study on the characteristics and crystal growth of GaSb (GaSb결정 성장과 특성에 관한 연구)

  • 이재구;오장섭;정성훈;송복식;문동찬;김선태
    • Electrical & Electronic Materials
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    • v.9 no.9
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    • pp.885-890
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    • 1996
  • Undoped p-type and Te doped n-type GaSb crystals were grown by the vertical Bridgman method. The lattice constant of the GaSb crystals was 6.096.+-.000373.angs.. The carrier concentration, the resistivity, and the carrier mobility measured by the van der Pauw method were p.iden.8*10$^{16}$ c $m^{-3}$ , .rho..iden.0.20 .ohm.-cm, .mu.$_{p}$ .iden.400c $m^{2}$ $V^{-1}$ se $c^{-1}$ for p-type, n.iden.1*10$^{17}$ c $m^{-3}$ , .rho..iden.0.15 .ohm.-cm, .mu.$_{n}$ .iden.500c $m^{2}$ $V^{-1}$ se $c^{-1}$ for n-type at 300K. In case of treatment with metal ion of R $u^{+3}$, P $t^{+4}$, the carrier concentration, resistivity and carrier mobility of the GaSb crystals were p.iden.2*10$^{17}$ c $m^{-3}$ , .rho..iden.0.08.ohm.-cm, .mu.$_{p}$ .iden.420c $m^{2}$ $V^{-1}$ se $c^{-1}$ for p-type, n.iden.2.5*10$^{17}$ c $m^{-3}$ , .rho..iden.0.07.ohm.-cm, .mu.$_{n}$ .iden.520c $m^{2}$ $V^{-1}$ se $c^{-1}$ for n-type respectively. GaSb crystals had a tendency to lower resistivity and higher mobility, for surface treatment with metal ion effectively diminished surface recombination centers.s.

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Creep Rupture Due to Molybdenum Rich $M_6C$ Carbide in 1.0Cr-1.0Mo-0.25V Steel Weldment (1.0Cr-1.0Mo-0.25V강 용접부의 $M_6C$ 탄화물에 의한 크립 파단)

  • O, Yeong-Geun;Kim, Byeong-Cheol;Gang, Gye-Myeong;Min, Tae-Guk
    • Korean Journal of Materials Research
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    • v.6 no.12
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    • pp.1257-1262
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    • 1996
  • 1.0Cr-1.0Mo-0.25V강 용접부의 크립 파단 시험시 파단 발생 원인에 관한 연구가 시행되었다. 파괴는 Intercritical Heat Affected Zone에서 발생하였으며 파단면에서 구상의조대한 M6C탄화물이 발견되었다. 모재는 molybdenum 주성분의 M2C, vanadium 주성분의 M4C3 및 chromium 주성분의 M23C6와 M7C3 탄화물이 존재하였다. 모의 실험 결과 준안정 상태인 M2C 탄화물은 85$0^{\circ}C$, 10oh에서 안정한 M6C탄화물로 변태하였다. M6C 탄화물은 주변의 molybdenum 농도를 떨어뜨려 강도의 저하를 가져오며 크립 기공의 발생 원인을 제공하였다.

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Consolidation Behavior of Soft Ground by Prefabricated Vertical Drains (연직드레인 공법에 의한 연약지반의 압밀거동)

  • 이달원;강예묵
    • Proceedings of the Korean Society of Agricultural Engineers Conference
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    • 1998.10a
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    • pp.376-381
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    • 1998
  • The large scaled field test by prefabricated vertical drains was performed to evaluate the superiority of vertical discharge capacity for drain materials through compare and analyze the time-settlement behavior with drain spacing and the compression index and consolidation coefficient obtained by laboratory experiments and field monitoring system 1. The relation of measurement settlement( $S_{m}$) versus design settlement( $S_{t}$) and measurement consolidation ratio( $U_{m}$) versus design consolidation ratio( $U_{t}$) were shown $S_{m}$=(1.0~l.1) $S_{t}$, $U_{m}$=(1.13~l.17) $U_{t}$, at 1.0m drain spacing and $S_{m}$=(0.7~0.8) $S_{t}$, $U_{m}$=(0.92~0.99) $U_{t}$ at 1.5m drain spacing, respectively. 2. The relation of field compression index( $C_{cfield}$) and virgin compression index( $V_{cclab}$) was shown $C_{cfield}$=(1.0~1.2) $V_{cclab}$, But it was nearly same value when considered the error with determination method of virgin compression index and prediction method of total settlement. 3. field consolidation coefficient was larger than laboratory consolidation coefficient, and the consolidation coefficient ratio( $C_{h}$/ $C_{v}$) were $C_{h}$=(2.4 ~ 3.0) $C_{v}$. $C_{h}$=(3.5 ~ 4.3) $C_{v}$ at 1.0m and 1.5m drain spacing and increased with increasing of drain spacingngasing of drain spacingng spacingng

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Studies on the survival Rate after Slow and Ultrarapid Frozen-Thawing of Porcine Embryos (돼지 수정란의 완만 및 초급속 동결 융해후의 생존성에 관한 연구)

  • 이봉구;김상근;이규승
    • Korean Journal of Animal Reproduction
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    • v.16 no.2
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    • pp.117-123
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    • 1992
  • This Study was carried out ot investigate the effects of concentration and equilibration time of cryoprotective aagents on survival rate of slowly and ultrarapidly frozen porcine embryos. The porcine embryos following dehydration by cryoprotective agents and 0.25M sucrose were slowly freezed(from 2$0^{\circ}C$ to -7$^{\circ}C$/-1$^{\circ}C$/min., from -7$^{\circ}C$ to -35$^{\circ}C$/-0.2$^{\circ}C$/min., from -35$^{\circ}C$ to -38$^{\circ}C$/-0.3$^{\circ}C$/min.) by Cell Freezer and directly plunged into liquid nitrogen and thawed in 38$^{\circ}C$ water bath. Survival rate was defined as development rate to the morula and blastocyst stage after in vitro culture or by FDA test. The results are summarized as follows : 1. The survival rates of porcine embryos after slow frozen-thawing in the freezing medium of 0.25M sucrose added 2.0M glycerol, 3.0M DMSO, 2.0M propanediol or 2.0M glycerol+2.0M propanediol was 80.6, 84.7, 75.0 or 78.8%, respectively. 2. The survival rates of porcine embryos after slow frozen-thawing in the freezing medium of 0.50M sucrose added 2.0M glycerol, 3.0M DMSO, 2.0M propanediol or 2.0M glycerol+2.0M propanediol was 80.9, 82.4, 73.1 or 77.1%, respectively. 3. The survival rates of porcine embryos after ultrarapid frozen-thawing in the freezing medium of 0.25M sucroese added 2.0M glycerol, 3.0M DMSO, 2.0M propanediol or 2.0M glycerol+2.0M propanediol was 65.3, 68.6, 63.2 or 59.9%, respectively. 4. The survival rates of porcine embryos after ultrapid frozen-thawing in the freezing medium of 0.50M sucrose added 2.0M glycerol, 3.0M DMSO, 2.0 propanediol or 2.0M glycerol+2.0M propanediol was 67.5, 62.9, 56.9, or 62.8%, respectively. 5. The higher survival rate of porcine embryos was attained at the short period ofequilibration time(5min.) in the freezing medium added 0.25M sucrose and 3.0 DMSO compared to those of 10 or 20min. equilibration time in the same condition.

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