• Asian-Australasian Journal of Animal Sciences
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• v.27 no.2
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• pp.155-160
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• 2014

The purpose of this study was to estimate genetic parameters of ultrasound measurements for longissimus dorsi muscle area (LMA), backfat thickness (BFT), and marbling score (MS) in Hanwoo cows (N = 3,062) at the ages between 18 and 42 months. Data were collected from 100 Hanwoo breeding farms in Gyeongbuk province, Korea, in 2007 and 2008. The cows were classified into four different age groups, i.e. 18 to 22 months (the first pregnancy period), 23 to 27 (the first parturition), 28 to 32 (the second pregnancy), and 33 to 42 (the second parturition), respectively. For each age group, a multi-trait animal model was used to estimate variance components and heritabilities of the three traits. The averages of LMA, BFT, and MS measurements across the cows of all age groups were 50.1 $cm^2$, 4.62 mm, and 3.04, respectively and heritability estimates were 0.09, 0.10, and 0.08 for the respective traits. However, when the data were analyzed in different age groups, heritability estimates of LMA and BFT were 0.24 and 0.47, respectively, for the cows of 18 to 22 months of age, and 0.21 for MS in the 28 to 32 months old cows. When the cows of all age groups were used, the estimates of genetic (phenotypic) correlations were 0.43 (0.35), -0.06 (0.34) and 0.21 (0.32) between LMA and BFT, LMA and MS, and BFT and MS, respectively. However, in the cow age group between 28 and 32 (18 and 22) months, the estimates of genetic (phenotypic) correlations were 0.05 (0.29), -0.15 (0.24) and 0.38 (0.24), for the respective pairs of traits. These results suggest that genetic, environmental, and phenotypic variations differ depending on cow age, such that care must be taken when ultrasound measurements are applied to selection of cows for meat quality.

• Analytical Science and Technology
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• v.25 no.6
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• pp.410-415
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• 2012

In this study, a fundamental study was conducted to establish the method for quantitative analysis and pretreatment of triclosan, one of the chemicals to be used as bactericides, detergents, and sterilizers. The extraction efficiency of triclosan was the highest when it was extracted by soxhlet apparatus with n-hexane, and it showed a satisfactory result when the samples were analyzed with both GC/ECD and GC/MS. To evaluate the reproductivity of the results, each 30 reference samples made with paper filter and cotton wool was prepared in this study. From the results with high extraction effectiveness, low analysis deviation, and good statistical analysis data, the method for the extraction and analysis for triclosan were resonable for the paper and textile samples applied in this study.

• International Journal of Concrete Structures and Materials
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• v.9 no.3
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• pp.345-367
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• 2015

Past earthquakes have signaled the increased collapse vulnerability of mainshock-damaged bridge piers and urgent need of repair interventions prior to subsequent cascading hazard events, such as aftershocks, triggered by the mainshock (MS). The overarching goal of this study is to quantify the collapse vulnerability of mainshock-damaged substandard RC bridge piers rehabilitated with different repair jackets (FRP, conventional thick steel and hybrid jacket) under aftershock (AS) attacks of various intensities. The efficacy of repair jackets on post-MS resilience of repaired bridges is quantified for a prototype two-span single-column bridge bent with lap-splice deficiency at column-footing interface. Extensive number of incremental dynamic time history analyses on numerical finite element bridge models with deteriorating properties under back-to-back MS-AS sequences were utilized to evaluate the efficacy of different repair jackets on the post-repair behavior of RC bridges subjected to AS attacks. Results indicate the dramatic impact of repair jacket application on post-MS resilience of damaged bridge piers-up to 45.5 % increase of structural collapse capacity-subjected to aftershocks of multiple intensities. Besides, the efficacy of repair jackets is found to be proportionate to the intensity of AS attacks. Moreover, the steel jacket exhibited to be the most vulnerable repair intervention compared to CFRP, irrespective of the seismic sequence (severe MS-severe or moderate AS) or earthquake type (near-fault or far-fault).

• Journal of the Institute of Electronics Engineers of Korea SD
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• v.49 no.3
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• pp.1-7
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• 2012

A 9b MS/s CMOS cyclic folding A/D converter (ADC) for intelligent battery sensor and battery management systems is proposed. The proposed ADC structure is based on a cyclic architecture to reduce chip area and power consumption. To obtain a high speed ADC performance, further, we use a folding-interpolating structure. The prototype ADC implemented with a 0.35um 2P4M n-well CMOS process shows a measured INL and DNL of maximum 1.5LSB and 1.0LSB, respectively. The ADC demonstrates a maximum SNDR and SFDR of 48dB and 60dB, respectively, and the power consumption is about 110mW at 2MS/s and 3.3V. The occupied active die area is $10mm^2$.

• Proceedings of the Korean Environmental Sciences Society Conference
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• 2006.11a
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• pp.285-289
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• 2006

In screening of algicidal bacteria, we isolated a marine bacterium which had potent algicidal effects on harmful algal bloom (HAB) species. This organism was identified as a strain very close to Bacillus subtilisby 16S rRNA gene sequencing. This bacterium, Bacillus sp. SY-1, produces very active algicidal compounds against the harmful dinoflagellate Cochlodinium polykrikoides. We isolated three algicidal compounds (MS 1056, 1070, 1084) and identified them by amino acid analyses, fast atom bombardment mass spectrometry (FAB-MS), infrared spectroscopy (IR), $^1H$, $^{13}C$, and extensive two-dimensional nuclear magnetic resonance (2D NMR) techniques including $^1H-^{15}N$ HMBC analysis. One of them, MS 1056, contains a b-amino acid residue with an alkyl side chain of $C_{15}$. MS 1056, 1070, and 1084 showed algicidal activities against C. polykrikoides with an $LC_{50}$ (6 hrs) of 2.3, 0.8, $0.6\;{\mu}g/ml$, respectively. These compounds also showed significant algicidal activities against other harmful dinoflagellates and raphidophytes. In contrast, MS 1084 showed no significant growth inhibition against various organisms coexisting with HAB species in natural environments, including bacteria, eukaryotic microalgae, and cyanobacteria, although it inhibited growth of some fungi and yeasts. These observations imply that algicidal bacterium Bacillus sp. SY-1 and its algicidal compounds could play an important role in regulating the onset and development of HABs in the natural environments.

• Asian-Australasian Journal of Animal Sciences
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• v.19 no.1
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• pp.86-90
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• 2006

This study was carried out to determine the effects of free choice feeding of starter feed and whole cereals on the growth of turkey poults. Two hundred-seventy poults (7-d old) were divided into 3 treatment groups each with 3 replications containing 15 males and 15 females. Control poults (C) were fed only on starter feed (280 g CP and 11.7 MJ ME/kg). Treatments for choice-fed turkey poults were offered a choice of a starter feed and wheat (120 g CP and 12.3 MJ ME/kg) for wheat selecting (WS) or maize (83 g CP and 13.9 MJ ME/kg) for maize selecting (MS) group in separate feeders. Final live weight (2,280.7 g for the C vs. 2,374.3 and 2,324.6 g for the WS and MS), daily gain (39.21 g vs. 40.87 and 39.99 g) and total feed intake (95.2 g vs. 103.2 and 97.9 g) in the WS poults were significantly higher (p<0.05) than those of the C group, but feed conversion ratio was not significantly different between the treatments. The proportional intakes of cereals vs. total feed intake were 11% in both the WS and MS group. The amount of protein intake in the C group (280 g CP/kg diet) was significantly (p<0.05) higher than that in WS and MS groups (263 and 259 g CP/kg diet, respectively) whilst the ME intake in the C group (11.7 MJ/kg diet) was significantly (p<0.05) lower than that in MS group (11.9 MJ/kg diet). Our results showed that turkey poults fed starter feed and whole wheat in a free choice feeding system have a higher efficiency in terms of daily gain, final live weight, and an economic advantage may be obtained from free choice feeding due to a reduced protein intake and feed cost.

• Archives of Pharmacal Research
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• v.27 no.2
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• pp.265-272
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• 2004

The pharmacokinetics of CKD-732 (6-0-4-[dimethyl-aminoethoxy)cinnamoyl]-fumagillolㆍhemioxalate) was investigated in male SD rats and beagle dogs after bolus intravenous administration. The parent compound and metabolites obtained from in vitro and in vivo samples were determined by LC/MS. The main metabolite was isolated and identified as an N-oxide form of CKD-732 by NMR and LC/MS/MS. CKD-732 was metabolized into either M11 or others by rapid hydroxylation, demethylation, and hydrolysis. The blood level following the intravenous route declined in first-order kinetics with $T_{1}$2/$\beta$ values of 0.72-0.78 h for CKD-732 and 0.92-1.09 h for M11 in rats at a dose of 7.5-30 mg/kg. In dogs, $T_{1}$2/$\beta$ values of CKD-732 and M11 were 1.54 and 1.79 h, respectively. Moreover, AUC values increased dose dependently for CKD-732 and M11 in rats and dogs. The CLtot and Vdss did not change significantly with increasing dose, indicating linear pharmacokinetic patterns. The excretion patterns through the urine, bile, and feces were also examined in the animals. The total amount excreted in urine, bile, and feces was 2.13% for CKD-732 and 1.29% for M11 in rats, and 1.58% for CKD-732 and 2.28% for M11 in dogs.

• Korean Journal of Community Nutrition
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• v.16 no.6
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• pp.762-770
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• 2011

Osteoprotegerin (OPG) plays a core role in bone reformation by antagonizing the effect of receptor activator of nuclear factor ${\kappa}$-B ligand (RANKL), and mediates vascular calcification in cardiovascular disease patients. Thus, we aimed to examine the relationship between serum OPG levels and cardiovascular factors and inflammatory markers in metabolic syndrome patients (MS). This cross-sectional study included 96 men who visited the diet clinic between May and July 2011. Patients were classified into 2 groups based on NCEP-ATP guidelines: normal and with MS (n = 50 and 46, respectively). Physical measurements, biochemical assay were measured. Serum OPG and IL-6, diponectin and hs-CRP were assessed. MS were aged $50.02{\pm}10.85$ years, and normal patients $52.07{\pm}9.56$ years, with no significant differences. Significant differences were not observed in BMI between the 2 groups. Moreover, significant differences were not observed in serum OPG, however, the serum OPG level ($4.41{\pm}1.86pmol/L$) differed significantly between an overweight MS (BMI > 25) and normal patients. OPG was correlated to age (r = 0.410, p = 0.000), HDL-cholesterol (r = 0.209, p = 0.015), and log adiponectin (r = 0.175, p = 0.042). Multiple regression analyses using the enter method showed that age (${\beta}$ = 0.412, p = 0.000) and BMI (${\beta}$ = 0.265, p = 0.000) considerably affected OPG. In conclusion, out study showed that serum OPG levels are correlated with cardiovascular risk factors, such as BMI, HDL-cholesterol and adiponectin in MS and adiponectin, suggesting that serum OPG has potential as a cardiovascular disease indicator and predictor.

• Journal of Plant Biotechnology
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• v.41 no.1
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• pp.1-9
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• 2014

So far it has been generally considered that proteomic approaches are very useful for studying plant-microbes interaction. In this review, recent studies based on papers published from 2010 to 2013 have investigated proteomics analysis in various interaction during plant-fungal pathogen infection by means of gel-based proteomics coupled with mass spectrometry (MS)-based analysis. In rice, three papers focused on rice-Magnaporthe oryzae interaction were mainly reviewed in this study. Interestingly, another study showed proteomic changes in rice inoculated with Puccinia triticina, which is not only an fungal pathogen in wheat and but also results to the disease resistance with non-host defense manner in rice. Additionally, proteomics analysis has been widely subjected to understand defense mechanism during other crops (wheat, tomato, strawberry and mint) and their fungal pathogen interaction. Crops inoculated are analyzed to identify differentially regulated proteins at various tissues such as leaf and apoplast using 2-DE analysis coupled with various MS approaches such as MALDI-TOF MS, nESI-LC-MS/MS and MudPIT, respectively. Taken together, this review article shows that proteomics is applicable to various organisms to understand plant-fungal pathogen interaction and will contribute to provide important information for crop disease diagnosis and crop protection.

• Journal of the Korean Society of Food Science and Nutrition
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• v.37 no.7
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• pp.834-840
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• 2008

In order to improve the use of pumpkin seed, the present study was performed to isolate compositions of the bitter components which were not seen in pumpkin seed itself but newly biosynthesized during germination of the seed. The compositions isolated were then further purified by TLC and preparative HPLC in which a fraction with Rf 0.73 and RT 10.3 was obtained. Cucurbitacin E with molecular weight of 557 from the fraction was finally identified by subsequent structural analysis of LC-MS/MS. The production of cucurbitacin E peaked with 224.7 mg/kg at 4 days of germination at $20^{\circ}C$ with the water supply at ntervals of 48 hrs in the darkness, while that of cucurbitacin E reached 146.7 mg/kg in the brightness. In vitro-cell based assays demonstrated that the isolated and purified cucurbitacin E inhibited proliferation of A549 lung cancer cells and suppressed expression of the IL-$1{\beta}$- or PMA-induced cyclooxygenase-2, an inflammatory protein in A549 cells, suggesting its anti-proliferative and anti-inflammatory activities.