• Proceedings of the Korea Water Resources Association Conference
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• 2015.05a
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• pp.403-403
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• 2015

본 연구에서는 강우시 도로지역에서 발생하는 비점오염물질 유출 및 수질특성을 살펴보고자 도로지역(국도 46호선)을 대상으로 총 16회의 강우사상에 대해 분석하였다. 모니터링 기간(2014년 5월~9월)동안 연구대상지역에는 3.2~80.3 mm의 강우가 발생하였으며, 강우강도는 0.39~11.29 mm/hr의 범위로 나타났다. 선행무강우일수는 0.3~20.1일이며, 총 유출량은 0.1~6.8 ㎥, 유출율은 0.24~0.85(평균 0.6)의 범위로 나타났다. 강우 모니터링 결과, 유량가중평균농도(Event Mean Concentration, EMC)는 TOC 4.9~56.2 mg/L(평균 18.0 mg/L), BOD 3.1~21.5 mg/L(평균 7.5 mg/L), COD 6.7~58.6 mg/L(평균 23.6 mg/L), SS 2.1~281.9 mg/L(평균 59.4 mg/L), T-N 1.07~13.46 mg/L(평균 4.89 mg/L) 그리고 T-P 0.065~0.680 mg/L(평균 0.23 mg/L)의 범위로 나타났으며, 강우계급별로 살펴보면 0~10 mm일 때 BOD 9.3 mg/L, COD 30.5 mg/L, SS 84.1 mg/L, T-N 5.42 mg/L, T-P 0.27 mg/L로, 10~30 mm일 때 BOD 6.6 mg/L, COD 22.0 mg/L, SS 29.0 mg/L, T-N 4.9 mg/L, T-P 0.20 mg/L로, 50 mm 이상의 강우에서는 BOD 3.6 mg/L, COD 7.1 mg/L, SS 46.4 mg/L, T-N 3.42 mg/L, T-P 0.10 mg/L로 강우계급별 EMC는 대부분 수질항목에 있어 10 mm 이하 계급의 평균 EMC가 높은 수준으로 나타났다. 그리고 각 강우사상에 대한 단위면적당 오염부하는 TOC 0.06~3.5 kg/ha, $BOD_5$ 0.03~1.6 kg/ha, CODMn 0.09~4.74 kg/ha, SS 0.09~35.99 kg/ha, T-N 0.012~2.600 kg/ha, T-P 0.001~0.062 kg/ha의 범위로 산정되었다. 도로지역은 불투수층 면적비율이 높아 누적강우량 10 mm 이하에서도 유출이 발생하는 것으로 분석되었으며, 작은 강우에도 초기유출이 발생하고 유출되는 오염물질 농도가 높은 경향을 나타내었다.

• KSBB Journal
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• v.19 no.5
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• pp.352-357
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• 2004

Human ferritin H- and L-chain genes (hfH and hfL) were cloned into the yeast shuttle vector YEp352 containing the GAL1 (galactokinase) and GAL10 (epimerase) divergent promoters and the vectors constructed were used to transform Saccharomyces cerevisiae 2805. SDS-PAGE displayed expression of the introduced hfH and hfL in both recombinant strains of Y1H10L and Y1L10H. The ferritin subunits, that represented ca. $22\%$ and $15\%$ of the soluble proteins in Y1H10L and Y1L10H, were spontaneously assembled into active ferritin heteropolymers. The H subunit content of the purified recombinant human ferritin heteropolymers was proven to reflect the relative expression yield of the subunits. When the cells of 2d culture were incubated with 14.3 mM Fe(2), the cellular iron concentration of Y1H10L and Y1L10H was 1.7 and 2.0 times, respectively, that of the control strain. It is assumed that increase in the iron uptake of the recombinant yeasts is closely related to ferritin expression and H subunit content.

• The Korean Journal of Physiology and Pharmacology
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• v.4 no.2
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• pp.99-104
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• 2000

We investigated the action of NOS inhibitors on NOS in rats. Both of nitric oxide synthase inhibitors, $N^G$-monomethyl-L-arginine $(L-NMMA,\;3\;{\mu}M)$ or $N^G$-nitro-L-arginine methylester $(L-NAME,\;30\;{\mu}M),$ augmented phenylephrine $(PE,\;10^{-7}\;M)-induced$ contraction which was inhibited by acetylcholine (ACh) in rat thoracic aorta. This augmentation by L-NAME or L-NMMA was attenuated with the treatment of NO precursor, arginine. ACh, however, decreased the augmentation induced by L-NMMA, but not by L-NAME. Superoxide dismutase (SOD, 50 u/ml) potentiated an inhibitory effect of ACh on the PE $(10^{-7}\;M)-induced$ contraction. It has been known that platelet activating factor itself induces iNOS. Platelet activating factor $(PAF,\;10^{-7}\;M)$ inhibited PE $(10^{-7}\;M)-induced$ contraction. Pretreatment with L-NMMA (30 mM) or L-NAME (30 mM) significantly blocked the inhibitory action of PAF on PE-induced contraction. L-NMMA (100 mM) or L-NAME (100 mM) reduced nerve conduction velocity (NCV) relevant to nNOS in rat sciatic nerve. ACh attenuated the reduction of NCV by L-NMMA-, but not by L-NAME-induced reduction of NCV. These results suggest that L-NMMA and/or L-NAME have different action on three types of NOS in rats.

• BMB Reports
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• v.53 no.8
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• pp.431-436
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• 2020

Alpha-fetoprotein (AFP) is one of the most commonly used and reliable biomarkers for Hepatocellular carcinoma（HCC). However, the underlying mechanism of AFP expression in HCC is poorly understood. In this study, we found that TCP10L, a gene specifically expressed in the liver, is down-regulated in HCC and that its expression inversely correlates with AFP expression. Moreover, overexpression of TCP10L suppresses AFP expression whereas knockdown of TCP10L increases AFP expression, suggesting that TCP10L might be a negative regulator of AFP. We found that TCP10L is associated with the AFP promoter and inhibits AFP promoter-driven transcriptional activity. Taken together, these results indicate that TCP10L negatively regulates AFP expression in HCC and that it could be a potential prognostic marker and therapeutic target for HCC.

• Journal of Life Science
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• v.13 no.4
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• pp.390-399
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• 2003

Four species of the genus of Listeria were isolated from seawater and sea food in Kyungnam province, South Korea. These isolated strains were classified into Listeria sp. from different samples by appropriate cultivation conditions and biochemical tests including serological test. In a day enrichment cultivation, the following strains were found out of 100 samples: L. innocua (35%), L. ivanovii (4%), L. monocytogenes (4%), and L. welshimeri (1%). For seven days enrichment culture, L. innocua (38%), L. ivanoii (5%), L. monocytogenes (7%), and L. welshimeri (1%) were isolated. From these results, Listeria species were more efficiently isolated in seven day enrichment broth than in one day enrichment. However, these isolated Listeria species were less grown in the selective medium than in the enrichment medium. Isolation rates of Listeria species showed differency for each sample and Listeria species were more abundantly isolated in shrimps (80%) and crayfishes (80%) than little neck clams (50%), seawater (25%) and mussels (20%). From the results of serological classes for the seven L. monocytogenes, two strains were defined as type I and the other five strains as type IV.

• The Journal of The Korea Institute of Intelligent Transport Systems
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• v.14 no.5
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• pp.38-43
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• 2015

In this paper, we present novel dual-band microstrip antennas using inverted-L-shaped parasitic elements vertically at radiation apertures for GPS L1(1.575 GHz) and L2(1.227 GHz) bands. For making dual band which has large interval, the inverted-L-shaped parasitic element was loaded at the radiation aperture of a half-wavelength patch antenna(GPS L1) in opposite direction of the feeding point for receiving the low frequency(GPS L2). The low frequency occurs by perturbation and coupling between the patch and parasitic. Next, due to use circular polarizations at the GPS applications, two inverted-L-shaped parasitic elements were loaded at radiation apertures of each polarizations and the feeding point was moved at diagonal part of the patch. The dimensions of the designed circularly polarized antenna were $88.5{\times}79{\times}10.4mm^3$ ($0.36{\lambda}L{\times}0.32{\lambda}L{\times}0.04{\lambda}L$, ${\lambda}L$ is the free-space wavelength at 1.227 GHz). Measured -10 dB bandwidths were 116.3 MHz(7.4%) and 64.3 MHz(5.2%) at GPS L1 and L2 bands, respectively. All of these cover the respective required system bandwidths. The measured 3 dB axial ratio bandwidths were 11.7 MHz(0.74%) and 14 MHz(1.14%), respectively. Within each of the designed bands, broadside radiation patterns were observed.

• Tuberculosis and Respiratory Diseases
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• v.45 no.2
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• pp.301-310
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• 1998

Background: Cell mediated immune response mediated by interaction between CD4+ T lymphocytes and macrophagies is thought to play an important role in tuberculous pleurisy. This interaction is dependent on the interplay of various cytokines. The immunologic response of tuberculous pleurisy is thought to depend on the balance between helper T cell(Th1) cytokine Interleukin-12, Interferon gamma and Th2 cytokine IL-4, IL-10. To understand immunologic mechanism in tuberculous pleurisy and evaluate diagnostic value of these cytokines, the concentrations of Th1 cytokine IL-12, IFN -$\gamma$ and Th2 cytokine IL-10 were measured in tuberculous pleurisy and malignant pleural effusion group. Material and Methods: The concentrations of IL-10, IL-12 and IFN-$\gamma$ were measured by ELISA method in pleural fluids and serums of 20 patients with tuberculous pleurisy and 20 patients with malignant pleural effusion ADA activities were measured by spetrophotomery in pleural fluids of both groups. Results: In tuberculous pleurisy, the mean concentrations of IL-10, IL-12 and IFN-$\gamma$ of pleural fluids showed $121.3{\pm}83.7$ pg/mL, $571.4{\pm}472.7$ pg/mL and $420.4{\pm}285.9$ pg/mL. These were significantly higher than that of serum, $21.2{\pm}60.9$ pg/mL, 194.5 pg/mL, $30.1{\pm}18.3$ pg/mL respectively(p< 0.01). In malignant pleural effusion, the mean concentrations of IL-10, IL-12 and IFN-$\gamma$ of pleural fluids showed $88.4{\pm}40.4$ pg/mL, $306.5{\pm}271.1$ pg/mL and $30.5{\pm}54.8$ pg/mL respectively. Compared with that of serum ($43.4{\pm}67.2$ pg/mL, $206.8{\pm}160.6$ pg/mL, $14.6{\pm}3.3$ pg/mL), only IL-10 was significantly higher (p<0.001), but IL-12, IFN-$\gamma$ were not significant. In tuberculous pleural effusion compared with malignant pleural effusion, the concentration of IL-12, IFN-$\gamma$, ADA were significantly higher (p=value 0.046, <0.001, <0.001), but IL-10 was not significant. For differential diagnosis of tuberculous pleurisy from malignant pleural effusion, using cut-off value of IL-12, IFN-$\gamma$, ADA as 300 pg/mL. 100 pg/mL, 45 U/L, the sensitivity/specificity were 60%/70%, 90%/87.5%, 85%/90% respectively. Conclusion: In tuberculous pleurisy, IL-10, IL-12 and IFN-$\gamma$ were selectively concentrated highly in pleural space than serum. Compared with malignant pleural effusion, IL-12 and IFN-$\gamma$ were significantly higher, but IL-10 were not in tuberculous pleural effusion. The results suggest that Th1 pathway contributes to immune resistant mechanism in tuberculous pleurisy. IFN-$\gamma$ and ADA revealed useful methods of differential diagnosis in tuberculous pleurisy from malignant pleural effusion.

• Journal of Practical Agriculture & Fisheries Research
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• v.4 no.1
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• pp.80-88
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• 2002

This study was carried out to investigate the native distribution and flowering characteristics of Lycoris genus which is endemic species in Asia. This study was summarized as fellows: Native distribution of Lycoris genus was situated in latitude 37- 24 degrees with high humidity of coastline. Mininum temperature of native area was at -10℃ during winter season. The leaf of L. squamigera, L. koreana, L. sangunea, L. sprengeri, L. incanata and L. flavescens emergenced in spring. The leaf of L. radiata, L. rdiata var pumila, L. aurea, L. traubii, L. albiflora and L. houdyshelli emergenced in autum. Bulb of Lycoris genus show a sympodial branching system which is composed of 14-23 scales and 2.8-5.2 leaves per each bulb at flowering time. The flower shape of L. squamigera, L. Koreana, L, aurea, L. incanata, L. sprengeri, L. sanguinea and L. flavescens was trumpet. The flower of L. radiata. L. radiata var pumila, L. albiflora, L. houdchelli and L. traubii. was spider.

• Food Science and Preservation
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• v.30 no.1
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• pp.132-145
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• 2023

In this study, culture conditions were optimized to confirm the feasibility of Acetobacter pasteurianus as a starter for fermentation vinegar. Acetobacter pasteurianus strain can be used as a food ingredient. The optimal temperature and pH conditions of the selected Acetobacter pasteurianus SRCM101388 were 28℃ and pH 6.00, respectively. The response surface methodology (RSM) was used to optimize the composition of the medium, and Plackett-Burman design (PBD) was used to obtain the effective selection of culture medium, resulting in that glucose, sucrose, and yeast extract had the highest effect on increasing biomass. The optimal concentration, which was performed by central composite design (CCD), were determined to be 10.73 g/L of glucose, 3.98 g/L of sucrose, and 18.73 g/L of yeast extract, respectively. The optimal concentrations of trace elements for the production of biomass were found to be 1 g/L of ammonium sulfate, 0.5 g/L of magnesium sulfate, 2 g/L of sodium phosphate monobasic, 2 g/L of sodium phosphate dibasic, and the final optimized medium was pH 6.10. When incubated in a 5 L jar fermenter, the SRCM101388 strain showed a faster-dissolved oxygen (DO) reduction at a lower agitation rate (rpm), and it was able to grow even at reduced DO level when aeration was maintained. The amount of final biomass produced was 2.53±0.12×10⁹ CFU/mL (9.40±0.02 log CFU/mL) when incubated for 18 hours at 150 rpm, 0.5 vvm, pH 6.0, and 28℃.

• Journal of Wetlands Research
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• v.14 no.1
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• pp.131-137
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• 2012

This study was investigated to characterize the stormwater runoff pollutant materials from the urban area mixed with industrial area. Almost the similar rainfall pattern is shown during the 5 years, and the yearly precipitation was 5.2~6.6 mm. The first flushing effect during the early-stage rainfall-run off was observed in some events. EMC ranges are 19.3~39.9 mg/L for BOD, 45.2~190 mg/L for CODcr, 67.2~351 mg/L for TSS, 3.6~10.3 mg/L for TN, 1.2~2.5 mg/L for TP. Heavy metal are not detected except Zn which is observed at only one event. The particle size was distributed to 10 ${\mu}m$ at the 3% weight volume and the 50% cumulative weight percent was shown at 12 ${\mu}m$.