KSBB Journal

The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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Iron Uptake by the Recombinant Yeasts Producing Ferritin Heteropolymers

재조합 효모에 의한 Ferritin 이형집합체의 발현과 철 흡수

  • Chang Yu Jung (Faculty of Biological Sciences, Institute for Molecular Biology and Genetics, Chonbuk National University) ;
  • Park Chung Ung (Faculty of Biological Sciences, Institute for Molecular Biology and Genetics, Chonbuk National University) ;
  • Kim Kyung Suk (Faculty of Biological Sciences, Institute for Molecular Biology and Genetics, Chonbuk National University)
  • 장유정 (전북대학교 자연대 생물과학부 및 유전공학연구소) ;
  • 박충웅 (전북대학교 자연대 생물과학부 및 유전공학연구소) ;
  • 김경숙 (전북대학교 자연대 생물과학부 및 유전공학연구소)
  • Published : 2004.10.01
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Abstract

Human ferritin H- and L-chain genes (hfH and hfL) were cloned into the yeast shuttle vector YEp352 containing the GAL1 (galactokinase) and GAL10 (epimerase) divergent promoters and the vectors constructed were used to transform Saccharomyces cerevisiae 2805. SDS-PAGE displayed expression of the introduced hfH and hfL in both recombinant strains of Y1H10L and Y1L10H. The ferritin subunits, that represented ca. $22\%$ and $15\%$ of the soluble proteins in Y1H10L and Y1L10H, were spontaneously assembled into active ferritin heteropolymers. The H subunit content of the purified recombinant human ferritin heteropolymers was proven to reflect the relative expression yield of the subunits. When the cells of 2d culture were incubated with 14.3 mM Fe(2), the cellular iron concentration of Y1H10L and Y1L10H was 1.7 and 2.0 times, respectively, that of the control strain. It is assumed that increase in the iron uptake of the recombinant yeasts is closely related to ferritin expression and H subunit content.

Keywords

References

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