• 한국수정란이식학회지
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• 제25권2호
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• pp.103-110
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• 2010

The first morphogenetic event of preimplantation development, compaction, was required efficient production of porcine embryos in vitro. Compaction of the porcine embryo, which takes place at post 4-cell stage, is dependent upon the adhesion molecule E-cadherin. The E-cadherin through ${\beta}$-catenin contributes to stable cell-cell adhesion. Rho-associated kinase (ROCK) signaling was found to support the integrity of E-cadherin based cell contacts. In this study, we traced the effects of ROCK-1 on early embryonic development and structural integrity of blastocysts in pigs. Then, in order to gain new insights into the process of compaction, we also examined whether ROCK-1 signaling is involved in the regulation of the compaction mediated by E-cadherin of cellular adhesion molecules. As a result, real-time RT-PCR analysis showed that the expression of ROCK-1 mRNA was presented throughout porcine preimplantation stages, but not expressed as consistent levels. Thus, we investigated the blastocyst formation of porcine embryos treated with LPA and Y27632. Blastocysts formation and their qualities in LPA treated group increased significantly compared to those in the Y27632-treated group (p < 0.05). Then, to determine whether ROCK-1 associates embryonic compaction, we explored the effect of activator and/or inhibitor of ROCK-1 on compaction of embryos in pigs. The rate of compacted morula in LPA treated group was increased compared to that in the Y27632-treated group (39.7 vs 12.0%). Furthermore, we investigated the localization and expression pattern of E-cadherin at 4-cell stage porcine embryos in both LPA- and Y27632-treated groups by immunocytochemical analysis and Western blot analysis. The expression of E-cadherin was increased in LPA-treated group compared to that in the Y27632-treated group. The localization of E-cadherin in LPA-treated group was enriched in part of blastomere contacts compared to that Y27632-treated group. ROCK-1 as a crucial mediator of embryo compaction may plays an important role in regulating compaction through E-cadherin of the cell adhesion during the porcine preimplantation embryo. We concluded that ROCK-1 gene may affect the developmental potential of porcine blastocysts through regulating embryonic compaction.

• 한국전자파학회논문지
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• 제18권7호
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• pp.839-846
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• 2007

본 논문에서는 새로운 구조의 이중 대역 feedforward 선형 전력 증폭기(FFW LPA)의 설계 방법을 제안하였다. 현재까지 이중 대역 FFW LPA 설계의 기술적인 어려움은 단일 대역 FFW LPA에서 사용되는 신호 상쇄기를 이중 대역 동작으로 확장시키기가 쉽지 않았다는 점이다. 따라서 본 논문에서는 이중 대역 동일 군속도 지연 주 신호 상쇄기와 혼변조 왜곡 신호(Intermodulation Distortion: IMD) 상쇄기의 설계 방법, 그리고 이를 이용하여 단일 대역 FFW 기법을 확장한 이중 대역 FFW LPA의 설계 방법을 제안하였다. 제안하는 설계 방식의 효용성을 검증하기 위하여, 동작 대역의 중심 주파수를 기준으로 1.26 GHz 이격된 디지털 셀룰러 대역($f_0=880$ MHz)과 IMT-2000($f_0=2.14$ GHz) 대역 기지국용 이중 대역 feedforward 선형화 시스템을 구현하였다. 각 대역별로 CDMA IS-95A 4FA 신호와 WCDMA 4FA 신호를 인가하여 인접 채널 누설비(Adjacent Channel Leakage Ratio: ACLR)의 개선 정도를 측정한 결과, 디지털 셀룰러 대역에서 평균 출력 전력 41.5 dBm일 때 16.52 dB, IMT-2000 대역에서 평균 출력 전력 40 dBm일 때 18.59 dB의 개선 효과를 동시에 얻을 수 있었다.

• 대한약학회:학술대회논문집
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• 대한약학회 2002년도 Proceedings of the Convention of the Pharmaceutical Society of Korea Vol.2
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• pp.96-97
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• 2002

Lysophosphatidic acid (LPA) and sphingosine 1-phosphate (SIP) are bioactive lysophospholipids (LPs) that act as mediators in various cellular processes, such as cell growth, differentiation, survival, motility, and cytoskeletal reorganization (1,2). LPA and S1P are both abundant in serum and are produced by activated platelets and other cell types. (omitted)

• Journal of Chest Surgery
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• 제47권1호
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• pp.32-34
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• 2014

Anomalous aortic origin of the left subclavian artery (LSCA) from the left pulmonary artery (LPA) is a rare congenital cardiac malformation. We describe a case of LSCA from the LPA via ductus arteriosus in association with a double-outlet right ventricle, which never has been reported previously in Korea.

• 대한의생명과학회지
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• 제12권1호
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• pp.9-16
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• 2006

We investigated the effects of 835-MHz electromagnetic field (EMF), one of the most popular communication frequency band in Korean code-division multiple-access (CDMA) mobile phone system, on cellular signal transduction. For this, we examined the change of intracellular calcium $([Ca^{2+}]_i)$, reactive oxygen species (ROS) and F-actin polymerization after exposure to 835-MHz EMF followed by the treatment of agonists in Rat-2 fibroblast cells. Culture cells were pretreated with serum-tree medium and concomitantly exposed to 835-MHz at specific absorption rate (SAR) of 4.0 W/kg for 24 hr in a specialized designed apparatus based on Transverse Electro Magnetics (TEM) wave theory. Intracellular $Ca^{2+}$ responses to lysophosphatidic acid (LPA) and epidermal growth factor (EGF) in Rat-2 fibroblast after exposure to 835-MHz EMF were shown to be similar pattern as observed in normal cultured cells. However, the LPA-induced calcium spiking was slightly delayed to 7 sec and sustained thereafter to a little higher ground level under 835-MHz EMF radiation compared to unexposed cells. ROS production level by LPA in the exposed cells was not different from that in control. Furthermore, LPA induced the production of stress fibers with no significant difference in the exposed and unexposed cells. These results suggest that mobile phone radiation (835-MHz, SAR 4.0 W/kg) may not be directly related to signal transduction in Rat-2 fibroblasts except the slight effect of calcium spiking in LPA-induced cells but remain to be further elucidated for possible indirect intervention.

• 창의정보문화연구
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• 제5권2호
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• pp.117-134
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• 2019

본 연구는 발명영재학생과 일반학생의 기질 및 성격을 비교하고, 나아가 발명영재집단의 기질 및 성격에 대한 LPA 분석을 통해 하위집단별 특성도 파악해 보고자 하였다. 이를 위해 본 연구는 다음과 같은 연구문제를 설정하였다. 첫째, 발명영재와 일반학생의 기질과 성격에는 차이가 있는가? 둘째, 발명영재들의 기질 및 성격 특성에 기반을 둔 잠재적 계층분류와 그 분류에 따른 특성은 무엇인가? 본 연구의 결과는 다음과 같다. 첫째, TCI 아동용 검사(JTCI)의 결과를 비교한 결과, 발명과학교육원에 입학한 학생들은 일반학생들보다 창조적이고 수용적이며, 대체로 긍정적인 감정을 자주 느끼는 것을 알 수 있었다. 특히 발명영재는 개인적인 욕구 이상의 의미 있는 목표와 가치를 추구하려는 동기가 높다는 것을 확인하였다. 둘째, 잠재계층프로파일분석(LPA)을 통해 발명영재 집단을 두 그룹으로 구분할 수 있었다. 한 그룹은 기질의 하위척도인 '자극추구'의 점수가 월등히 높았고, 성격의 하위척도인 '자율성'에서 약간 높은 점수를 나타냈다. 다른 그룹은 '자극추구'의 점수가 일반학생과 비슷하면서 '자율성'에서 월등히 높은 점수를 나타냈다.

• 한국전자파학회논문지
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• 제11권8호
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• pp.1407-1413
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• 2000

본 논문에서는 기지국용 전력 증폭기의 혼 변조 성분들을 극도로 억압하기 위해서 Error feedback 방식을 사용한 Feedforward 선형 전력 증폭기를 설계하였다. 특히, 제안된 선형 전력 증폭기는 기존의 피드백 기술의 단점인 증폭 이득의 손실을 없앤 Error feedback 기술과 Feedforward 기술을 혼합하여 적용하였다. 제안된 선형 전력 증폭기는 HP사의 EEsof ADS ver.1.3을 사용해서 설계.제작하였다. 측정결과, 이득 28 ㏈, P1㏈ 31 ㏈m인 주 전력 증폭기에 -7 ㏈m/tone을 갖는 1850 MHz와 1851.25 MHz의 2-tone 신호를 인가한 결과 전체 IMD가 약 35 ㏈ 개선되었다.

• Journal of Ginseng Research
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• 제45권3호
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• pp.390-400
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• 2021

Background: We recently showed that gintonin, an active ginseng ingredient, exhibits antibrain neurodegenerative disease effects including multiple target mechanisms such as antioxidative stress and antiinflammation via the lysophosphatidic acid (LPA) receptors. Amyotrophic lateral sclerosis (ALS) is a spinal disease characterized by neurodegenerative changes in motor neurons with subsequent skeletal muscle paralysis and death. However, pathophysiological mechanisms of ALS are still elusive, and therapeutic drugs have not yet been developed. We investigate the putative alleviating effects of gintonin in ALS. Methods: The G93A-SOD1 transgenic mouse ALS model was used. Gintonin (50 or 100 mg/kg/day, p.o.) administration started from week seven. We performed histological analyses, immunoblot assays, and behavioral tests. Results: Gintonin extended mouse survival and relieved motor dysfunctions. Histological analyses of spinal cords revealed that gintonin increased the survival of motor neurons, expression of brain-derived neurotrophic factors, choline acetyltransferase, NeuN, and Nissl bodies compared with the vehicle control. Gintonin attenuated elevated spinal NAD(P) quinone oxidoreductase 1 expression and decreased oxidative stress-related ferritin, ionized calcium-binding adapter molecule 1-immunoreactive microglia, S100β-immunoreactive astrocyte, and Olig2-immunoreactive oligodendrocytes compared with the control vehicle. Interestingly, we found that the spinal LPA1 receptor level was decreased, whereas gintonin treatment restored decreased LPA1 receptor expression levels in the G93A-SOD1 transgenic mouse, thereby attenuating neurological symptoms and histological deficits. Conclusion: Gintonin-mediated symptomatic improvements of ALS might be associated with the attenuations of neuronal loss and oxidative stress via the spinal LPA1 receptor regulations. The present results suggest that the spinal LPA1 receptor is engaged in ALS, and gintonin may be useful for relieving ALS symptoms.

• Journal of Ginseng Research
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• 제45권3호
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• pp.401-407
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• 2021

Background: Gintonin is an exogenous ginseng-derived G-protein-coupled lysophosphatidic acid (LPA) receptor ligand. LPA induces in vitro morphological changes and migration through neuronal LPA1 receptor. Recently, we reported that systemic administration of gintonin increases blood-brain barrier (BBB) permeability via the paracellular pathway and its binding to brain neurons. However, little is known about the influences of gintonin on in vivo neuron morphology and migration in the brain. Materials and methods: We examined the effects of gintonin on in vitro migration and morphology using primary hippocampal neural precursor cells (hNPC) and in vivo effects of gintonin on adult brain neurons using real time microscopic analysis and immunohistochemical analysis to observe the morphological and locational changes induced by gintonin treatment. Results: We found that treating hNPCs with gintonin induced morphological changes with a cell rounding following cell aggregation and return to individual neurons with time relapses. However, the in vitro effects of gintonin on hNPCs were blocked by the LPA1/3 receptor antagonist, Ki16425, and Rho kinase inhibitor, Y27632. We also examined the in vivo effects of gintonin on the morphological changes and migration of neurons in adult mouse brains using anti-NeuN and -neurofilament H antibodies. We found that acute intravenous administration of gintonin induced morphological and migrational changes in brain neurons. Gintonin induced some migrations of neurons with shortened neurofilament H in the cortex. The in vivo effects of gintonin were also blocked by Ki16425. Conclusion: The present report raises the possibility that gintonin could enter the brain and exert its influences on the migration and morphology of adult mouse brain neurons and possibly explains the therapeutic effects of neurological diseases behind the gintonin administration.

• 한국환경복원기술학회지
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• 제20권1호
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• pp.131-142
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• 2017

The function of urban parks as wildlife habitats is becoming increasingly important. The urban park serves as a key area for preserving urban biodiversity. The purpose of this study is to estimate the bird species diversity in 30 parks in Cheonan city and quantitatively analyze the influence of vegetation, park structure and landscape connectivity index. As the results, a total of 27 birds species and 1,509 individuals were found at the sites and the largest number of birds were found in the Cheongsa park with 17 species. The optimal regression model was selected as the explanatory variables for the logged park area (LPA), the tree cover ratio (Co_T) and the patch betweenness centrality (PB). LPA and Co_T mean the internal characteristics of the park, and PB was the external environmental variable meaning landscape connectivity. LPA was the most important factor (73.3%) as bird habitat, and the PB could be interpreted as a factor that should be considered as important (26.7%). It will be possible to consider these environmental variables in the park and green area construction and management.