• 한국자원식물학회지
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• 제29권6호
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• pp.635-641
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• 2016

Sweet potato (Ipomoea batatas L.) shoot tips grown in vitro were successfully cryopreserved by encapsulation-vitrification. Encapsulated explants are very easily manipulated, due to the relatively large size of the alginate beads, and a large number of samples can be treated simultaneously. In this study, the effects of sucrose preculture, cryoprotectant preculture, and post-warm recovery media on regrowth, following liquid nitrogen (LN) exposure, were investigated to establish an efficient encapsulation-vitrification protocol for sweet potato. Shoot tips of plants grown in vitro were precultured in 0.3 M sucrose for 2 d before encapsulation. Encapsulated shoot tips were pre-incubated in liquid MS (Murashige and Skoog) medium containing 0.5 M sucrose for 16 h, before preculturing in sucrose-enriched medium (0.7 M sucrose) for 8 h. Shoot tips were osmoprotected with 35% plant vitrification solution 3 (PVS3) for 3 h, before being dehydrated with PVS3 for 2 h at $25^{\circ}C$. The encapsulated and dehydrated shoot tips were transferred to 2 mL cryotubes, suspended in 0.5 mL PVS3, and plunged directly into liquid N. High levels of shoot formation were obtained for the cv. Yeulmi (65.7%) and Yeonwhangmi (80.3%). The regrowth rates of cryopreserved samples in Yeulmi (78.9%) and Yeonwhangmi (91.3%), following culture on ammonium-free MS medium for 5 d, were much higher than those cultured on standard MS medium (65.7% and 80.3%, respectively). This encapsulation-vitrification is a promising method for the long-term preservation of sweet potato.

• 대한기계학회:학술대회논문집
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• 대한기계학회 2007년도 춘계학술대회B
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• pp.2321-2326
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• 2007

To return LNG to a gaseous state, it is fed into a regasification plant. For inland area, where pipelines do not exist or difficult to construct, the LNG is delivered to the inland-receiving terminal available at that area, regasified and delivered to consumers. At inland-receiving terminal, air-heating vaporizer type is usually used. To contribute in developing an efficient air-heating vaporizer, experiment on finned type air-heating vaporizer using 8 fins, 50 mm fin length (8fin50le) and 4 fins, 75 mm fin length (4fin75le) were conducted. The experiments were conducted by varying the ambient condition and the length of the vaporizer. The ambient air was controlled so that it has the same condition with air condition in every season available. $LN_2$ is used to substitute LNG because of safety reason. The results show that characteristics of the finned type 4fin75le vaporizer are comparable to finned type 8fin50le vaporizer at all season.

• Ocean and Polar Research
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• 제33권3호
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• pp.303-308
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• 2011

Polar psychrophiles which thrive under extreme conditions such as cold temperature, high salinity, and high dose ultraviolet light, emerge as novel targets for biotechnology. To prevent genetic drift and the possibility of contamination by subculturing, cryopreservation was employed for two psychrophilic microalgae, Porosira sp. (KOPRI AnM0008) and Pyramimonas sp. (KOPRI AnM0046), which have anti-freeze activities. Five cryoprotectants (dimethyl sulphoxide, ethylene glycol, glycerol, methanol and propylene glycol) showed toxicity at 20-30% (v/v). The optimal cryoprotectant concentration and equilibration time were less than 20% and 10 min, respectively. Cryopreservation was carried out in the presence of cryoprotectants either by direct freezing in liquid nitrogen ($LN_2$) or controlled freezing using a controlled rate freezer followed by storage in the $LN_2$ tank. As a result, Pyramimonas sp. (KOPRI AnM0046), a psychrophilic chlorophyta was revived. Cryopreserved Porosira sp. was not revived from either freezing protocols probably due to the silicic cell wall and its relatively large cell size. In the case of Pyramimonas sp. (KOPRI AnM0046), the controlled freezing method showed higher revival yield than the direct freezing method.

• 한국초전도ㆍ저온공학회논문지
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• 제20권1호
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• pp.19-22
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• 2018

The thermal and electrical properties of the conductor are critical parametersfor the design and optimization of the superconducting magnet. This paper presents simulation code to analyze electrical and thermal stability characteristics of the second generation (2G) high-temperature superconductor (HTS) by varying copper stabilizer thickness. Two types of commercial 2G HTS coated conductor tapes, YBCO and GdBCO were used in this study. These samples were cooled by Liquid Nitrogen ($LN_2$) having boiling at 77.3 K and an equivalent electrical circuit model for them is choosen and analysed in details. Also, an over-current pulse test in which a current exceeding a critical current was performed. From the simulation results, the influences of the copper stabilizer thickness on the stability characteristics of these samples are presented.

• 한국산림과학회지
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• 제95권5호
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• pp.585-590
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• 2006

In vitro-grown axillary shot-tip meristems of Lycium chinense Mill. from cold-acclimated plant were successfully cryopreserved using a vitrification technique. After loading for 15 minutes with a mixture of 2.0 M glycerol and 0.4 M sucrose ($20^{\circ}C$), small segments (1-2 mm, 3-4 mm, and 5-6 mm) were cut from axilary buds and exposed to the cryoprotectant solution containing 30% glycerol, 15% ethylen glycol,15% dimethyl sulfoxide (DMSO), and 0.4 M sucrose at $0^{\circ}C$ for 30-120 minutes prior to direct plunge into liquid nitrogen (LN). After rapid thawing ($40^{\circ}C$), the segments were washed with MS medium containing 1.2 M sucrose for 0-35 minutes, and then transferred onto recovery-growth medium. The highest survival rate (about 90%) was obtained with cold-hardening treatment, and cryopreserved explants were successfully recovered to plantlets. No abnormal morphological changes were observed with the recovered plants after cryopreservation.

• 한국정밀공학회지
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• 제34권4호
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• pp.237-241
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• 2017

Cryogenic machining uses liquid nitrogen (LN2) as a coolant. This machining process can reduce the cutting temperature and increase tool life. Titanium alloys have been widely used in the aerospace and automobile industries because of their high strength-to-weight ratio. However, they are difficult to machine because of their poor thermal properties, which reduce tool life. In this study, we applied cryogenic machining to titanium alloys. Orthogonal cutting experiments were performed at a low cutting speed (1.2 - 2.1 m/min) in three cooling conditions: dry, cryogenic, and cryogenic plus heat. Cutting force and friction coefficients were observed to evaluate the machining characteristics for each cooling condition. For the cryogenic condition, cutting force and friction coefficients increased, but decreased for the cryogenic plus heat condition.

• 대한전기학회:학술대회논문집
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• 대한전기학회 2006년도 제37회 하계학술대회 논문집 B
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• pp.723-724
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• 2006

In this study, superconducting coil arrangements and cryostat concept design were conducted for the development of 13.2kV/630A bifilar winding type high temperature superconducting(HTS) fault current limiter(FCL) with YBCO coated conductor(CC) wire. The coil consists of several layers with unique non-inductive solenoid winding method. Six types of HTS coil arrangements were investigated for the optimal insulation design of HTS FCL. And, conceptual design of cryostat was conducted for the decrement of thermal invasion and the prevention of low voltage insulation breakdown in the LHe which is used as pressurization gas in sub-cooling condition of liquid nitrogen(LN2). As the results, it was found that the modified suspended type cryostat with horizontal coil arrangement is beneficial to the insulation design of 13.2kV level bifilar winding type HTS FCL.

• 한국기계가공학회지
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• 제18권2호
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• pp.96-101
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• 2019

This paper presents a machinability evaluation of Inconel 718 material when using a new uniform-temperature indirect cooling method that incorporates constant-pressure liquid nitrogen (LN2). The flank wear of a TiAlN coated tool used with this indirect cooling system was much lower than that of the tool used with dry machining under all machining conditions. Also, the surface roughness resulting from machining with this indirect method was far less than that of a dry machined surface after the same cutting time. Reduced heat generation and uniform temperature in turning operations play important roles in tool life and surface quality.

• Journal of Plant Biotechnology
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• 제8권1호
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• pp.43-50
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• 2006

The goal of this research was to develop an efficient cryopreservation protocol for germplasms of yam (Diosorea batatas), that were cultivated in Korea. Comparative studies with four other cryogenic techniques and subsequent experiments for shoot regrowth were conducted. in vitro-grown shoot-apices of the D. batatas were successfully cryopreserved by encapsulation-dehydration. The maximum survival of shoot-apices could be achieved when the precultured (with 0.3 M of sucrose for one day) and encapsulated (with a 3%(w/v) Na-alginate solution) apices were dehydrated for $3.5{\sim}4\;h$ prior to direct immersion in LN (liquid nitrogen). The frequency of regrowth rate of cryopreserved apices was not decreased during 3-month storage period. The thawing method markedly affected survival of the cryopreserved apices, and thawing at $40^{\circ}C$ for 3 min produced the best results. When cryopreserved apices were post-cultured on the post-culture medium (MS), supplemented with $0.2mgl^{-1}$ of BA ($N_6$-benzyladenine) and $0.2mgl^{-1}$ of kinetin, they showed direct shooting without callusing.

• Journal of Plant Biotechnology
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• 제45권4호
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• pp.333-339
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• 2018

The mature zygotic embryos of the Hevea brasiliensis were cryopreserved through the use of the vitrification and encapsulation/dehydration techniques. In all the experiments, the zygotic embryos were pre-cultured for three days in the MS medium supplemented with 0.3 M sucrose before they were used for the cryopreservation technique. In the vitrification procedure, the effect of the plant vitrification solutions (PVS2 and PVS3) and exposure time were studied. The highest survival rate (88.87%) and regrowth (66.33%) were achieved when the precultured zygotic embryos were incubated in a loading solution for 20 minutes at $0^{\circ}C$. They were subsequently exposed to PVS2 for 120 minutes at $0^{\circ}C$ and plunged directly into liquid nitrogen. Cryopreservation by the encapsulation-dehydration method was successfully done by leaving the encapsulated zygotic embryos in a laminar flow for 4 hours prior to plunging into a LN. The survival rate and regrowth of the encapsulated zygotic embryos were 37.50% and 27.98%, respectively. The cryopreserved zygotic embryos were able to develop into whole plants.