• Journal of Power System Engineering
• /
• v.11 no.3
• /
• pp.22-28
• /
• 2007

Liquefied gas vaporizer is a machine to vaporize liquefied gas such as liquid nitrogen($LN_{2}$), liquefied natural gas(LNG), liquid oxygen($LO_{2}$) etc. For the air type vaporizer, the frozen dew is created by temperature drop (below 273 K) on vaporizer surface. The layer of ice make a contractions on vaporizer. The structure analysis on the heat transfer was studied to see the effect of geometric parameters of the vaporizer, which are length 1000 mm of various type vaporizer. Structure analysis result such as temperature variation, thermal stress and thermal strain have high efficiency of heat emission as increase of thermal conductivity. As the result, Frist, With-fin model shows high temperature distribution better than without-fin on the temperature analysis. Second, Without-fin model shows double contractions better then with-fin model under the super low temperature load on the thermal strain analysis. Third, Vaporizer fin can be apply not only heat exchange but also a stiffener of structure. Finally, we confirm that All model vaporizer can be stand for sudden load change because of compressive yield stress shows within 280 MPa on thermal stress analysis.

• Korean Journal of Plant Resources
• /
• v.33 no.6
• /
• pp.689-695
• /
• 2020

This study was conducted to improve and supplement the system of cryopreservation for adventitious bulbs induced by tissue cultured bulb-scales of lily (Lilium spp.) cvs. 'Milky way'. The explants, bulblets and bulb-scale-bulblets, were treated to low temperature (4℃) for 7 days prior to the pre-culture. The adventitious bulbs were pre-cultured in Murashige and Skoog (MS) liquid medium supplemented with sucrose (0.3 and 0.7M). The pre-cultured adventitious bulbs were treated to loading solution (LS1 or LS2, C4 or C6) containing 35% of PVS3 (LS1, C4) or 40% of PVS3 (LS2, C6) for 40 min and exposed to dehydration solution (PVS3, B1) containing 50% glycerol and 50% sucrose for 60 min at 25℃. The adventitious bulbs were moved onto droplets containing 3 µl PVS3 on sterilized aluminum foils, and then soaked into liquid nitrogen (LN) for 60 min. The result of highest regrowth rate as 65.7% was obtained in cold treatment (4℃), osmoprotected with LS1 solution, and cultured in PCM3 medium by using bulb-scale-bulblet for cryopreservation. This result shows that droplet-vitrification could be used as a promising method for long-term storage of lily genetic resource.

• Proceedings of the Plant Resources Society of Korea Conference
• /
• 2021.04a
• /
• pp.35-35
• /
• 2021

This study was conducted to improve and supplement the system of cryopreservation for adventitious bulbs induced by tissue cultured bulb-scales of lily (Lilium spp.) cvs. 'MilkyWay'. The explants, bulblets and bulb-scale-bulblets, were treated to low temperature (4℃) for 7 days prior to the pre-culture. The adventitious bulbs were pre-cultured in Murashige and Skoog (MS) liquid medium supplemented with sucrose (0.3 and 0.7M). The pre-cultured adventitious bulbs were treated to loading solution (LS1 or LS2, C4 or C6) containing 35% of PVS3 (LS1, C4) or 40% of PVS3 (LS2, C6) for 40 min and exposed to dehydration solution (PVS3, B1) containing 50% glycerol and 50% sucrose for 60 min at 25℃. The adventitious bulbs were moved onto droplets containing 3 ㎕ PVS3 on sterilized aluminum foils, and then soaked into liquid nitrogen (LN) for 60 min. The result of highest regrowth rate as 65.7% was obtained in cold treatment (4℃), osmoprotected with LS1 solution, and cultured in PCM3 medium by using bulb-scale-bulblet for cryopreservation. This result shows that droplet-vitrification could be used as a promising method for long-term storage of lily genetic resource.

• Progress in Superconductivity and Cryogenics
• /
• v.15 no.3
• /
• pp.20-23
• /
• 2013

In the development of high temperature superconducting (HTS) power machines, HTS bushing is one of core technologies. In particular, the insulation body with sheds and electrical insulation at cryogenic temperature have attracted a great deal of interest from the view point of the size, weight and efficiency of bushing. In this study, the electrical and mechanical characteristics of various insulators for body in liquid nitrogen ($LN_2$) were investigated. And the surface discharge distance, collar length of GFRP sheds were studied. To emit bubbles between sheds, the shape and arrangement of shed were studied. The shed structure for 60 kV class HTS bushing were designed with regular arrangement.

• Journal of Embryo Transfer
• /
• v.26 no.3
• /
• pp.187-193
• /
• 2011

This study was carried out to establish most suitable freezing condition, to evaluate the different glycerol concentration of freezing and thawing rates on motility, viability, membrane integrity and acrosome intecrity of frozen Korean Jeju Black Bull spermatozoa, Semen was collected from a Korean Jeju Black Bull using an artificial vagina and transported to the laboratory. The semen was extended gradually 1:5 then cooled slowly for 2 hrs to 4$^{\circ}C$. The semen was diluted 1:1 with cryoprotectant extenders (3%, 5% and 7% glycerol) and equilibrated for 2 hrs at cold chamber and packed to 0.5 ml straws. The semen straws were located above 3 cm of liquid nitrogen for 5 minutes, above 5 cm for 10 min and above 8 cm for 10 min. And then the frozen straw was plunged into LN$_2$. The presented straws were examined the viability and motility after thawed at 37$^{\circ}C$ water bath. The viability and membrane integrity immediately post-thawing were significantly higher in samples frozen in 7% glycerol than 3% and 5% glycerol (p<0.05). After CTC staining to assess acrosome integrity, F pattern was significantly increased, but B pattern was significantly decreased in 7% glycerol (p<0.05). Freezing distance of 5 cm from liquid nitrogen and pre-cooling for 10 min yield better survival and membrane integrity, but not significant difference. However, AR pattern according to CTC staining was significantly decreased in 3 cm for 5 min.

• Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
• /
• 2004.05b
• /
• pp.87-93
• /
• 2004

Laminated Polypropylene Paper (LPP) and Kraft paper were used as ac power insulation for conventional cable as well as high temperature superconducting (HTS) cable because of its prominent insulating characteristics. However, researches on the use of LPP/Kraft paper in HTS cables are thinly scattered. In this paper, the effect of laminate polypropylene paper on the breakdown strength of LPP/Kraft multi-layer sample impregnated with liquid nitrogen (LN2)under ac and impulse applied voltage was studied. In addition, the breakdown strength characteristics of LPP and Kraft multi-layer sample were also investigated. It was found from the experimental data that the LPP has higher breakdown strength value than Kraft paper in ac and impulse. Especially in the ac case, the breakdown strength increases as the component ratio of LPP in the LPP/Kraftsample increases and slightly affected by the inserting position of LPP but in impulse case, the breakdown strength strongly depends on the number of LPP and the relative position of LPP.

• Korean Journal of Plant Resources
• /
• v.15 no.2
• /
• pp.89-95
• /
• 2002

In woody plant germplasms, using prefrozen dormant buds for materials is one way to achieve successful cryopreservation. The protocol of cryopreservation for White birch (Betula platyphylla var. japonica) winter vegetative buds is the following. First, the branches of White birch were collected in January 20, when the vegetative buds were still in a state of quiescence. The winter buds with about 5㎜ of xylem tissue were removed from the branches. They were dehydrated to moisture contents about 44% by air dry treatment. The buds were prefrozen, with the temperature being decreased by 5∼-20$\^{C}$ and then transfered to the LN(liquid nitrogen) maintained below -l96$\^{C}$. After cryopreservation, the vegetative buds were rapidly thawed in a water bath at 40$\pm$5$\^{C}$. In this case, the cell survival rate of samples was about 86%. After sterilization, buds were then cultured on MS medium. These results demonstrate the feasibility for cryopreservation of winter vegetation buds of Betula platyphylla var. japonica.

• Plant Biotechnology Reports
• /
• v.2 no.2
• /
• pp.123-131
• /
• 2008

Vitrification methods are convenient for cryopreserving plant specimens, as the specimens are plunged directly into liquid nitrogen (LN) from ambient temperatures. However, tissues and species with poor survival are still not uncommon. The development of vitrification solutions with high survival that cover a range of materials is important. We attempted to develop new vitrification solutions using bromegrass cells and found that VSL, comprising 20% (w/v) glycerol, 30% (w/v) ethylene glycol, 5% (w/v) sucrose, 10% (w/v) DMSO and 10 mM $CaCl_2$, gave the highest survival following cryopreservation, as determined by fluorescein diacetate staining. However, the cryopreserved cells showed little regrowth, for unknown reasons. To check its applicability, VSL was used to cryopreserve gentian axillary buds and the performance was compared with those of conventional vitrification solutions. Excised gentian stem segments with axillary buds (shoot apices) were two-step precultured with sucrose to induce osmotic tolerance prior to cryopreservation. Gentian axillary buds cryopreserved using VSL following the appropriate preculturing approach exhibited 78% survival (determined by the regrowth capacity), which was comparable to PVS2 and PVS1 and far better than PVS3. VSL had a wider optimal incubation time (20-45 min) than PVS2 and was more suitable for cryopreserving gentian buds. The optimal duration of the first step of the preculture was 7-11 days, and preculturing with sucrose and glucose gave a much higher survival than fructose and maltose. VSL was able to vitrify during cooling to LN temperatures, as glass transition and devitrification points were detected in the warming profiles from differential scanning calorimetry. VSL and its derivative, VSL+, seem to have the potential to be good alternatives to PVS2 for the cryopreservation of some materials, as exemplified by gentian buds.

• Korean Journal of Plant Resources
• /
• v.31 no.6
• /
• pp.684-694
• /
• 2018

This study describes the successful establishment of a cryopreservation protocol for Citrus limon cultivars: 'Frost Eureka limon' and 'Cook Eureka limon', using a droplet-vitrification method. The shoot tips that were excised from in vitro grown seedlings of the two cultivars were preserved in liquid nitrogen (LN) and successfully regenerated into whole plants. Excised shoot tips were pre-cultured for 1 or 2 days in 0.3 M and 0.5 M sucrose solutions at $25^{\circ}C$ and incubated in a loading solution (LS) composed of 17.5% glycerol + 17.5% sucrose in Murashige and Skoog (MS) medium for 40 min at $25^{\circ}C$. Prior to direct immersion in LN for 1 h, the shoot tips were dehydrated with plant vitrification solution 2 (PVS2) at $0^{\circ}C$ or PVS3 at $25^{\circ}C$. The frozen shoot tips were re-warmed and unloaded with 1.2 M sucrose in $\text\tiny{^1/_2}$ MS for 30 min at $25^{\circ}C$. Shoot tips were post-cultured overnight on survival medium and then micrografted onto 'trifoliate orange' (Poncirus trifoliate (L.) Raf. seedling rootstocks for recovery and to produce whole plants. The highest regrowth rates were 53.5% and 50.3% for cryopreserved shoot tips of 'Frost Eureka limon' and 'Cook Eureka limon', respectively, when pre-cultured in 0.3 M and 0.5 M sucrose concentrations in a sequencing manner, with LS and treated with PVS2 for 60 min at $0^{\circ}C$. We also investigated whether the ammonium ion concentration on post-culture medium affected the viability of the cryopreserved Citrus shoot tips. The viability of cooled samples, following culturing on woody plant media (WPM) containing $\text\tiny{^1/_4}$ ammonium nitrate overnight before micrografting, was the highest (70.3%) in 'Frost Eureka limon'. The study described here is a cost-effective and safe method to conserve Citrus fruit cultivars, for the improvement and large-scale multiplication of fruit plants and for breeding disease resistance.

• The Transactions of The Korean Institute of Electrical Engineers
• /
• v.57 no.3
• /
• pp.398-402
• /
• 2008

Recently, the development of superconducting fault current limiters (SFCLs) has been required as power demands increase in the power system. A distribution-level prototype resistive SFCL using coated conductor (CC) has been developed by Hyundai Heavy Industries Co., Ltd. and Yonsei University for the first time in the world. The ratings of the SFCL are 13.2kV/630A at normal operating condition. A novel non-inductive winding method is used in fabricating coils so there is almost zero impedance during normal operation. The distribution SFCL is cooled by sub-cooled liquid nitrogen $(LN_2)$ of 65K and 3 bar to enhance cryo-dielectric performance, critical current density, and thermal conductivity. In order to make reliable operation of an SFCL in real power systems, we monitored and controled its operation conditions by using supervisory control and data acquisition (SCADA) method. Thus, a monitoring system for the SFCL employing information technology (IT) is proposed and developed to be on the lookout for the operation conditions such as inside temperature, inside pressure, $LN_2$ level, voltage and current. Since operation temperature should be kept constant, bang-bang control for temperature feedback with a heater attached to the cold head of cryo-cooler is applied to the system. Short-circuit tests with prospective fault current of 10kA and AC dielectric withstand voltage tests up to 143kV for 1 minute were successfully performed at Korea Electrotechnology Research Institute. This paper deals with the development of a distribution level SFCL and its monitoring system for reliable operation.