• Title/Summary/Keyword: $K_m$ value

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Isolation and Characterization of α-Glucosidase Inhibitor Produced by Bacillus sp. SKU31-1 Strain (Bacillus sp. SKU31-1가 생산하는 α-Glucosidase 저해제 분리 및 특성 조사)

  • Kim, Shin-Duk
    • Korean Journal of Microbiology
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    • v.50 no.4
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    • pp.381-383
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    • 2014
  • In the course of screening for ${\alpha}$-glucosidase inhibitor produced by microorganism, the active compound was isolated from the culture filtrate of Bacillus sp. SKU31-1 using a series of chromatography procedures. The structure of the active compound was elucidated as 5-amino-1-hydroxymethyl-1, 2, 3, 4-cyclohexanetetrol on the basis of spectroscopic evidence obtained and comparison with data from the literature. The active compound showed potent inhibitory activity against ${\alpha}$-glucosidase with an $IC_{50}$ value of $1.9{\mu}M$ for maltose and 4.9 mM for sucrose. A Lineweaver-Burk plot indicated that its inhibition of ${\alpha}$-glucosidase was competitive, with a $K_i$ value of 0.15 mM.

Improvement of Cooperation Charge on Conservation of Ecosystem Reflected Natural Capitals Valuation - Focused on Forest Area - (자연자산의 가치를 반영한 생태계보전협력금 제도 개선 방안 - 산림지역을 중심으로 -)

  • Park, Jin-Han;Lee, Dong-Kun;Tanaka, Riwako;Kim, Jung-Taek;Jeon, Seong-Woo;Lee, Woo-Kyun;Kim, Joon-Soon;Jung, Tae-Yong
    • Journal of the Korean Society of Environmental Restoration Technology
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    • v.17 no.1
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    • pp.203-213
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    • 2014
  • In Korea, a cooperation charge on conservation of ecosystem is imposed to prevent indiscriminate development. The total amount of this charge is determined by multiplying the size of the destroyed area by a value per unit area and the area index within 5 billion won. Since 2001, the charge per unit area has been determined to be $250won/m^2$. In this study, we estimated the unit value of ecosystem services per year using benefit transfer method, with a focus on forest resources. According to our results, forest resources have a value of about $3,500won/m^2$ per year. When the non-use value is subtracted, that figure becomes approximately $1,300won/m^2$. If this value incorporates the unit value of the cooperation charge on conservation of ecosystem, it will increase. To comply with the original intent of the cooperation charge on conservation of ecosystem, the fund must be used to improve ecosystem services, including the restoration of a destroyed area or the purchase of new land.

Investigation on the Distnbution of the Groundwater at paho-Dong Area (지하수 수자원 분포에 관한 조사연구 (경북 달성군 파호동을 중심으로))

  • 나인엽
    • Water for future
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    • v.12 no.2
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    • pp.36-42
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    • 1979
  • This study has been conducted to find out the location and amount of the subterrain water body developed in the alluvial stratum in Paho-Dong, Sungsee-Myo-n, Dalsung-Kun are. An earlier test drilling was done in this area by R.O.K. Agricultural Promotion Corporation. The area consists of a small river basin and surrounding low hills developed around the junction of the Nakdong and Kumho Rivers. The strata of this area are made of Paldal gravel, Bokhyundong and Banyawol layers which were formed in the cretaceous period of the Meso-saicera or acid dikes and covered with-irregular alluvial layers. The alluvial layer in this area is composed of rather minute particles and proportional electric resistance tests on this layer show $10^2\;-\;10^3\;\Omega/cm$. The drillings up to 12meters deep showed only the sand layer (Form 3 to 26meters in thickness) contains water. The sand layers can not be considered a good water trapping one. Applying the data from the drillings to A.Hazen's equation, $K\;=\;{cd_e}^2\;(0.7\;+0.03t)$ to get the theoretical value of the water infilterated, I calculated it as K=13.92m/day. And again the value was set to Dupuit equation, (equation omitted) to acquire the pumping water amount the result was $Q_1\;=\;77.20\;\textrm{m}^3/day$. When the data-applied to the equation for pumping water amount, (equation omitted), the results were $Q_2\;=\;122.39\;\textrm{m}^3/day$ and K = 38m/day $Q_1\;and;Q_2$ (tow types of pumping water amount) represent proper value decrease and maximum value decrease respectively. Therefore, $Q_2$ is the least amount of water we can pump. The area covers about $1,555,000\;\textrm{m}^2$ and the maximum water needed in this area amounts to $155,000\textrm{m}^3$. That means we have to drill 1,406 pumping wells. It is concluded that undertaking the project in this area is irrational or even desperate and surface water should be developed more favorably.

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Control of Potato Late blight (Phytophthora infestans) with Postassium Phosphonate (아인산염의 감자 역병 방제효과)

  • Hong, Soon-Yeong;Lee, Kwang-Seok;Kang, Yong-Kil;Jee, Hyeong-Jin
    • Research in Plant Disease
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    • v.9 no.3
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    • pp.179-182
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    • 2003
  • Effect of potassium phosphonate on control of potato late blight was evaluated at two fields in Jeju island. The chemical showed 82.5% control value in field located at low seacoast with 100 m elevation, while dimethomorph copper oxychloride showed 75.9 % control value. However, its control value was only 40% in another field located at mid-hilly area with 300 m elevation, in which environmental conditions of high hummudity and often rainfall were favorable to the disease development. Application intervals of the phosphonate from 7 to 15 effectively suppressed the disease and did not show statistically different control values among the spraying intervals. Results indicated that phosphonate similarly or more effectively controls potato late blight than fungicide, however, its control value could be vared according to enviromental conditions for the disease development and 15-d spraying intervals ware sufficient to suppress ther disease.

OPTIMAL CONTROL OF A QUEUEING SYSTEM WITH $P^M_{\lambda}$-SERVICE POLICY

  • Kim, Sung-Gon;Bae, Jong-Ho
    • East Asian mathematical journal
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    • v.24 no.1
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    • pp.45-55
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    • 2008
  • We consider an M/G/1 queue with $P^M_{\lambda}$-service policy, which is a two-stage service policy. The server starts to serve with rate 1 if a job arrives to the sever in idle state. If the workload of the system upcrosses $\lambda$, then the service rate is changed to M and this rate continues until the system is empty. It costs to change the service rate to M and maintaining the rate. When the expectation of the stationary workload is supposed to be less than a given value, we derive the optimal value of M.

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Cytyotoxicity and Anti-Malassezia Activity of Limonene (Limonene의 세포독성과 항Malassezia 활성)

  • Lee, Jeong-Hyun;Lee, Jae-Sug
    • Microbiology and Biotechnology Letters
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    • v.39 no.4
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    • pp.387-389
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    • 2011
  • A previous study of ours indicated that Citrus auranifoli oil possesses antifungal activity against Malassezia furfur and Malassezia pachydermatis. In this study, we evaluated the anti-M. furfur and M. pachydermatis activities of limonene, which is a major component of C. aurantifolia oil, using the disk diffusion method. We also examined cytotoxicity against human normal epithelial (Beas-2B) cells using the cytopathic effect reduction (CPE) method. The results revealed that the minimum fungicidal concentration (MFC) value of limonene is lower than the value for itraconazole. The MFC value of limonene was seen to be 7.81 ${\mu}g$/mL against M. furfur and 3.90 ${\mu}g$/mL against M. pachydermatis. MFC values of itraconazole against M. furfur and M. pachydermatis were 62.50 ${\mu}g$/mL and 31.25 ${\mu}g$/mL, respectively. In addition, it was noted that limonene was not toxic to Beas-2B cells with normal morphology at a concentration of 100 ${\mu}g$/mL. However, itraconazole exhibited weak toxicity at the same concentration. Therefore, our results indicate that limonene could potentially be effective at controlling M. furfur and M. pachydermatis infections with no cytotoxicity.

Effect of Austenitizing Temperatures on the Mechanical Properties in 420J2 Martensitic Stainless Steel (420J2 마르텐사이트계 스테인레스강의 오스테나이트화 온도가 기계적 성질에 미치는 영향)

  • Kim, Y.G.;Kim, Y.J.;Kim, G.D.;Sung, J.H.
    • Journal of the Korean Society for Heat Treatment
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    • v.5 no.2
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    • pp.111-121
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    • 1992
  • For the purpose of investigating the effect of austenitizing temperatures on the mechanical properties of 0.23% C-13.6%Cr martensitic stainless steel, tensile properties, hardness, impact value and carbide extraction were examined after changing the austenitizing temperatures and tempering temperatures. The results obtained are summerized as follows. The carbide laminations formed from hot rolling before austenitization could not be eliminated after austenitizing at $950^{\circ}C$. With increasing austenitizing temperature, hardness increased and showed maximum value at $1050^{\circ}C$ and then slightly decreased. With increasing tempering temperature up to $500^{\circ}C$, impact value and elongation appeard to be decreased but hardness showed nearly unchanged at austenitizing temperature of $1150^{\circ}C$ due to the fine $M_7C_3$ carbides precipitation. The abrupt increase in impact value, hardness and elongation above the tempering temperature of $500^{\circ}C$ appeared to change in carbide structure from fine $M_7C_3$ to coarse $M_{23}C_6$.

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Dietary Value and Cryopreservation of the Isochrysis galbana used Shellfish Artificial Seed Production (패류 인공종묘 생산시 이용되는 Isochrysis galbana의 냉동보존 및 먹이효과)

  • Kang, Kyoung-Ho;Kim, Young-Hun;An, Seung-Jin;Han, Chan-Bae;Park, Hae-Joong
    • The Korean Journal of Malacology
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    • v.23 no.1
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    • pp.51-57
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    • 2007
  • This study examined the possibility on the dietary value and cryopreservation of the marine microalga, Isochrysis galbana. Four cryoprotectants, dimethyl sulfoxide (DMSO), ethylene glycol (EG), glycerol (Gly) and 1.2-propanedial (PD) were tested at the concentrations of 1.0, 1.5, 2.0, 2.5 and 3.0 M respectively. The highest survival rates were obtained with 1.5 and 2.0 M of four cryoprotectants yielding a survival rate of 80%. Cell concentration of $30\;{\times}\;10^4\;cell/ml$ at the initial point of the experiment was increased to $365\;{\times}\;10^4\;cell/ml$ in 1.5 M of dimethyl sulfoxide, $298\;{\times}\;10^4\;cell/ml$ in 1.5 M of ethylene glycol, $512\;{\times}\;10^4\;cell/ml$ in 1.5 M of glycerol, and $385\;{\times}\;10^4\;cell/ml$ in 1.5 M of 1.2-propanedial after five days, respectively. In dietary value experiment, survival rate and growth were not significantly different.

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POSITIVE SOLUTIONS FOR MULTIPOINT BOUNDARY VALUE PROBLEMS WITH ONE-DIMENSIONAL p-LAPLACIAN OPERATOR

  • Xu, Fuyi;Meng, Zhaowei;Zhao, Wenling
    • Journal of applied mathematics & informatics
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    • v.26 no.3_4
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    • pp.457-469
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    • 2008
  • In this paper, we study the existence of positive solutions for the following nonlinear m-point boundary value problem with p-Laplacian: $\{{{{(\phi_p(u'))'\;+\;f(t,u(t))=0, \;0<t<1,} \atop u'(0)={\sum}{^{m-2}_{i=1}}\;a_iu'(\xi_i),} \atop u(1)={\sum}{^k_{i=1}}\;b_iu(\xi_i)\;-\;{\sum}{^s_{i=k+1}}\;b_iu(\xi_i)\;-\;{\sum}{^{m-2}_{i=s+1}}\;b_iu'(xi_i),}$ where ${\phi}_p(s)$ is p-Laplacian operator, i.e., ${\phi}_p(s)=\mid s\mid^{p-2}s$, p>1, ${\phi}_q\;=\;({\phi}_p)^{-1}$, $\frac{1}{p}+\frac{1}{q}=1$, $1\;{\leq}\;k\;{\leq}\;s\;{\leq}m\;-\;2$, $b_i\;{\in}\;(0,+{\infty})$ with $0\;<\;{\sum}{^k_{k=1}}\;b_i\;-\;{\sum}{^s_{i=k+1}}\;b_i\;<\;1$, $0\;<\;{\sum}{^{m-2}_{i=1}}\la_i\;<\;1$, $0\;<\;{\xi}_1\;<\;{\xi}_2\;<\;{\cdots}\;<\;{\xi}_{m-2}\;<\;1$, $f\;{\in}\;C([0,\;1]\;{\times}\;[0,\;+{\infty}),\;[0,\;+{\infty}))$. We show that there exists one or two positive solutions by using fixed-point theorem for operator on a cone. The conclusions in this paper essentially extend and improve the known results.

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