• Title/Summary/Keyword: $I_{ks}$

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Study on the unification between KS I ISO standard and official test method enacted by Korean Ministry of Environment - drinking water and indoor air quality - (환경오염공정시험기준과 KS ISO규격의 일원화에 관한 연구 - 먹는 물 및 실내공기질 -)

  • Lee, Jeong-Il;Lee, Ju-Hee;Lee, Jeong-Hee;Lee, Jun-Hee;Lee, Won-Seok;Kim, Ji-In;Kim, Bo-Kyung;Choi, Sung-Hun
    • Analytical Science and Technology
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    • v.25 no.2
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    • pp.102-113
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    • 2012
  • Our study researched on unification of KS I ISO standard harmonized with ISO and Official Test Method enacted by Korean Ministry of Environment-drinking water and indoor air quality. We reviewed KS methods related to drinking water and indoor air quality for about 23,000 KS methods. KS methods related environmental field are classified as KS I, total 635 methods and 583 KS I methods were harmonized with ISO. For Environmental Standard methods for drinking water, 100 methods were reviewed according to 232 KS methods related to ISO/ TC 147 "Water Quality". Environmental Standard methods for indoor air quality were reviewed according to 95 KS standard methods related to ISO/TC 146 "Air Quality". By reviews and comparison tests for unifiable ES for drinking water and indoor air quality with KS methods harmonized with ISO, it was evaluated that for 100 ES methods for drinking water, 23 ES methods were unification complete, 29 ES methods were unification possible, 12 ES methods were unification impossible, no corresponding methods were found in KS I ISO for 36 ES methods and for 17 ES methods for indoor air quality,1 ES methods were unification complete, 3 ES methods were unification possible, 3 ES methods were impossible, no corresponding methods were found in KS I ISO for 10 ES methods.

Ginseng Gintonin Activates the Human Cardiac Delayed Rectifier K+ Channel: Involvement of Ca2+/Calmodulin Binding Sites

  • Choi, Sun-Hye;Lee, Byung-Hwan;Kim, Hyeon-Joong;Jung, Seok-Won;Kim, Hyun-Sook;Shin, Ho-Chul;Lee, Jun-Hee;Kim, Hyoung-Chun;Rhim, Hyewhon;Hwang, Sung-Hee;Ha, Tal Soo;Kim, Hyun-Ji;Cho, Hana;Nah, Seung-Yeol
    • Molecules and Cells
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    • v.37 no.9
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    • pp.656-663
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    • 2014
  • Gintonin, a novel, ginseng-derived G protein-coupled lysophosphatidic acid (LPA) receptor ligand, elicits $[Ca^{2+}]_i$ transients in neuronal and non-neuronal cells via pertussis toxin-sensitive and pertussis toxin-insensitive G proteins. The slowly activating delayed rectifier $K^+$ ($I_{Ks}$) channel is a cardiac $K^+$ channel composed of KCNQ1 and KCNE1 subunits. The C terminus of the KCNQ1 channel protein has two calmodulin-binding sites that are involved in regulating $I_{Ks}$ channels. In this study, we investigated the molecular mechanisms of gintonin-mediated activation of human $I_{Ks}$ channel activity by expressing human $I_{Ks}$ channels in Xenopus oocytes. We found that gintonin enhances $I_{Ks}$ channel currents in concentration- and voltage-dependent manners. The $EC_{50}$ for the $I_{Ks}$ channel was $0.05{\pm}0.01{\mu}g/ml$. Gintonin-mediated activation 1 of the $I_{Ks}$ channels was blocked by an LPA1/3 receptor antagonist, an active phospholipase C inhibitor, an $IP_3$ receptor antagonist, and the calcium chelator BAPTA. Gintonin-mediated activation of both the $I_{Ks}$ channel was also blocked by the calmodulin (CaM) blocker calmidazolium. Mutations in the KCNQ1 $[Ca^{2+}]_i$/CaM-binding IQ motif sites (S373P, W392R, or R539W)blocked the action of gintonin on $I_{Ks}$ channel. However, gintonin had no effect on hERG $K^+$ channel activity. These results show that gintonin-mediated enhancement of $I_{Ks}$ channel currents is achieved through binding of the $[Ca^{2+}]_i$/CaM complex to the C terminus of KCNQ1 subunit.

Differential effects of ginsenoside metabolites on slowly activating delayed rectifier K+ and KCNQ1 K+ channel currents

  • Choi, Sun-Hye;Lee, Byung-Hwan;Kim, Hyeon-Joong;Jung, Seok-Won;Hwang, Sung-Hee;Nah, Seung-Yeol
    • Journal of Ginseng Research
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    • v.37 no.3
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    • pp.324-331
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    • 2013
  • Channels formed by the co-assembly of the KCNQ1 subunit and the mink (KCNE1) subunit underline the slowly activating delayed rectifier $K^+$ channels ($I_{Ks}$) in the heart. This $K^+$ channel is one of the main pharmacological targets for the development of drugs against cardiovascular disease. Panax ginseng has been shown to exhibit beneficial cardiovascular effects. In a previous study, we showed that ginsenoside Rg3 activates human KCNQ1 $K^+$ channel currents through interactions with the K318 and V319 residues. However, little is known about the effects of ginsenoside metabolites on KCNQ1 $K^+$ alone or the KCNQ1 + KCNE1 $K^+$ ($I_{Ks}$) channels. In the present study, we examined the effect of protopanaxatriol (PPT) and compound K (CK) on KCNQ1 $K^+$ and $I_{Ks}$ channel activity expressed in Xenopus oocytes. PPT more strongly inhibited the $I_{Ks}$ channel currents than the currents of KCNQ1 $K^+$ alone in concentration- and voltage-dependent manners. The $IC_{50}$ values on $I_{Ks}$ and KCNQ1 alone currents for PPT were $5.18{\pm}0.13$ and $10.04{\pm}0.17{\mu}M$, respectively. PPT caused a leftward shift in the activation curve of $I_{Ks}$ channel activity, but minimally affected KCNQ1 alone. CK exhibited slight inhibition on $I_{Ks}$ and KCNQ1 alone $K^+$ channel currents. These results indicate that ginsenoside metabolites show limited effects on $I_{Ks}$ channel activity, depending on the structure of the ginsenoside metabolites.

Inhibition of LPS induced iNOS, COX-2 and cytokines expression by kaempferol-3-O-${\beta}$-D-sophoroside through the $NF{-\kappa}B$ inactivation in RAW 264.7 cells (Kaempferol-3-O-${\beta}$-D-sophoroside의 RAW 264.7 세포에서 $NF{-\kappa}B$ 억제를 통한 LPS에 의해 유도되는 iNOS, COX-2 및 cytokine들의 발현 저해효과)

  • Park, Seung-Jae;Shin, Ji-Sun;Cho, Woong;Cho, Young-Wuk;Ahn, Eun-Mi;Baek, Nam-In;Lee, Kyung-Tae
    • Korean Journal of Pharmacognosy
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    • v.39 no.2
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    • pp.95-103
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    • 2008
  • In the present study, we investigated the anti-inflammatory effects by kaempferol-3-O-${\beta}$-D-sophoroside (KS) isolated from Sophora japonica (Leguminosae) on the lipopolysaccharide (LPS)-induced nitric oxide (NO) and prostaglandin ($PGE_2$) production by RAW 264.7 cell line compared with kaempferol. KS significantly inhibited the LPS-induced NO and $PGE_2$ production. Consistent with these observations, KS reduced the LPS-induced expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) at the protein and mRNA levels in a concentration-dependent manner. In addition, the release and the mRNA expression levels of tumor necrosis $factor-{\alpha}$ ($TNF-{\alpha}$) and interleukin-6 (IL-6) were also reduced by KS. Moreover, KS attenuated the LPS-induced activation of nuclear factor-kappa B ($NF{-\kappa}B$), a transcription factor necessary for pro-inflammatory mediators, iNOS, COX-2, $TNF-{\alpha}$ and IL-6 expression. These results suggest that the down regulation of iNOS, COX-2, $TNF-{\alpha}$, and IL-6 expression by KS are achieved by the downregulation of $NF{-\kappa}B$ activity, and that is also responsible for its anti-inflammatory effects.

A Study on the Conformity of KS Standards according to Agreement on WTO/TBT (WTO/TBT 협정에 따른 KS 규격의 부합화에 관한 연구)

  • 김진규
    • Journal of Korean Society of Industrial and Systems Engineering
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    • v.26 no.4
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    • pp.12-22
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    • 2003
  • The purposes of this study are to investigate the conformity of Korean Standards(KS) according to agreement on WTO/TBT, and to propose systematic frameworks of preparation, adoption, and application for KS in our enterprises. Significant changes in this establishment, revision, and abrogation include the following divisions; i) statistics-vocabulary and symbols, ii) Shewhart control chart, iii) statistical interpretation of data, iv) sampling procedures for inspection by attributes, v) sequential sampling plans for inspection.

Survey on the Application of Standards and Specifications in Munitions Companies (I) (군수업체에서의 표준.규격 활용실태 조사 연구 (I))

  • Choi, Ki-In
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.11 no.12
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    • pp.4774-4779
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    • 2010
  • Based on the survey for munitions companies, we have tried to find current state of the application of Korean military specifications(KDS) and their references for munition products, and how they can be improved for a better management system. On average, Korean munitions companies have 660 of KS, 18 of U.S. military specifications, and 5 of foreign standards, and annually spend about 0.4 million won for KS, but 1.3 million won for foreign standards. In addition, they have emphasized the necessity of the integrated management system for KDS and the related drawings.

Effect of Differences between Korean Ministry of Environment Standard and ISO Standard on Determination of Petroleum Hydrocarbon Content in Soil (토양오염공정시험기준과 국제표준간의 분석방법 차이가 유류 정량에 미치는 영향)

  • Jung, In-Ho;Lee, Goon-Taek;Lee, Won-Seok;Kim, Yong-Hoon;Kim, Bo-Hyun;Kim, Ji-In;Kim, Bo-Kyong
    • Journal of Soil and Groundwater Environment
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    • v.17 no.4
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    • pp.63-72
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    • 2012
  • The main objective of this study is to assess the compatibility between Korean ministry of environment (KME) standard and ISO (KS I ISO) standard for the determination of BTEX and TPH content in soil. We carried out comparison analysis for both methods using CRM and matrix spiked samples. In case of GC-MS analysis for BTEX, we got statistically (significance level: 0.05) the same results from KME standard (ES 07600.1) and ISO standard (KS I ISO 15009). However, it showed statistically (significance level: 0.05) different results when TPH was analyzed by KME standard (ES 07552.1) and ISO standard (KS I ISO 16703). To clarify the reason why both methods produced different results for TPH content, we also did some additional experiments in terms of differences in extraction, clean-up and target hydrocarbon range. Extraction with polar and non-polar compounds mixed solvent (acetone+n-heptane) of KS I ISO 16703 showed higher extraction efficiency than with only non polar solvent (dichloromethane) extraction of ES 07552.1 by about 9%. While column type clean-up of KS I ISO 16703 showed the reduction in TPH content between before and after clean-up, batch type of clean-up of ES 07552.1 did not show any changes in TPH content through clean-up process. The target hydrocarbon range of ES 07552.1 and KS I ISO 16703 is $C_8{\sim}C_{40}$ and $C_{10}{\sim}C_{40}$, respectively. From this point of view, kerosene and JP-8 contaminated soil showed higher RPD (relative producibility deviation) values between results by both method than that of lubricant or diesel contaminated soil. The higher content of hydrocarbon ($C_8{\sim}C_{10}$) in kerosene and JP-8 played an important role in increasing RPD values in addition to the effects caused by different solvents and clean-up method. Consequently, it was concluded that both methods (ES 07552.1 and KS I ISO 16703) were not compatible.

A Study on the Development of a Simulation Model for Predicting Soil Moisture Content and Scheduling Irrigation (토양수분함량 예측 및 계획관개 모의 모형 개발에 관한 연구(I))

  • 김철회;고재군
    • Magazine of the Korean Society of Agricultural Engineers
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    • v.19 no.1
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    • pp.4279-4295
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    • 1977
  • Two types of model were established in order to product the soil moisture content by which information on irrigation could be obtained. Model-I was to represent the soil moisture depletion and was established based on the concept of water balance in a given soil profile. Model-II was a mathematical model derived from the analysis of soil moisture variation curves which were drawn from the observed data. In establishing the Model-I, the method and procedure to estimate parameters for the determination of the variables such as evapotranspirations, effective rainfalls, and drainage amounts were discussed. Empirical equations representing soil moisture variation curves were derived from the observed data as the Model-II. The procedure for forecasting timing and amounts of irrigation under the given soil moisture content was discussed. The established models were checked by comparing the observed data with those predicted by the model. Obtained results are summarized as follows: 1. As a water balance model of a given soil profile, the soil moisture depletion D, could be represented as the equation(2). 2. Among the various empirical formulae for potential evapotranspiration (Etp), Penman's formula was best fit to the data observed with the evaporation pans and tanks in Suweon area. High degree of positive correlation between Penman's predicted data and observed data with a large evaporation pan was confirmed. and the regression enquation was Y=0.7436X+17.2918, where Y represents evaporation rate from large evaporation pan, in mm/10days, and X represents potential evapotranspiration rate estimated by use of Penman's formula. 3. Evapotranspiration, Et, could be estimated from the potential evapotranspiration, Etp, by introducing the consumptive use coefficient, Kc, which was repre sensed by the following relationship: Kc=Kco$.$Ka+Ks‥‥‥(Eq. 6) where Kco : crop coefficient Ka : coefficient depending on the soil moisture content Ks : correction coefficient a. Crop coefficient. Kco. Crop coefficients of barley, bean, and wheat for each growth stage were found to be dependent on the crop. b. Coefficient depending on the soil moisture content, Ka. The values of Ka for clay loam, sandy loam, and loamy sand revealed a similar tendency to those of Pierce type. c. Correction coefficent, Ks. Following relationships were established to estimate Ks values: Ks=Kc-Kco$.$Ka, where Ks=0 if Kc,=Kco$.$K0$\geq$1.0, otherwise Ks=1-Kco$.$Ka 4. Effective rainfall, Re, was estimated by using following relationships : Re=D, if R-D$\geq$0, otherwise, Re=R 5. The difference between rainfall, R, and the soil moisture depletion D, was taken as drainage amount, Wd. {{{{D= SUM from { {i }=1} to n (Et-Re-I+Wd)}}}} if Wd=0, otherwise, {{{{D= SUM from { {i }=tf} to n (Et-Re-I+Wd)}}}} where tf=2∼3 days. 6. The curves and their corresponding empirical equations for the variation of soil moisture depending on the soil types, soil depths are shown on Fig. 8 (a,b.c,d). The general mathematical model on soil moisture variation depending on seasons, weather, and soil types were as follow: {{{{SMC= SUM ( { C}_{i }Exp( { - lambda }_{i } { t}_{i } )+ { Re}_{i } - { Excess}_{i } )}}}} where SMC : soil moisture content C : constant depending on an initial soil moisture content $\lambda$ : constant depending on season t : time Re : effective rainfall Excess : drainage and excess soil moisture other than drainage. The values of $\lambda$ are shown on Table 1. 7. The timing and amount of irrigation could be predicted by the equation (9-a) and (9-b,c), respectively. 8. Under the given conditions, the model for scheduling irrigation was completed. Fig. 9 show computer flow charts of the model. a. To estimate a potential evapotranspiration, Penman's equation was used if a complete observed meteorological data were available, and Jensen-Haise's equation was used if a forecasted meteorological data were available, However none of the observed or forecasted data were available, the equation (15) was used. b. As an input time data, a crop carlender was used, which was made based on the time when the growth stage of the crop shows it's maximum effective leaf coverage. 9. For the purpose of validation of the models, observed data of soil moiture content under various conditions from May, 1975 to July, 1975 were compared to the data predicted by Model-I and Model-II. Model-I shows the relative error of 4.6 to 14.3 percent which is an acceptable range of error in view of engineering purpose. Model-II shows 3 to 16.7 percent of relative error which is a little larger than the one from the Model-I. 10. Comparing two models, the followings are concluded: Model-I established on the theoretical background can predict with a satisfiable reliability far practical use provided that forecasted meteorological data are available. On the other hand, Model-II was superior to Model-I in it's simplicity, but it needs long period and wide scope of observed data to predict acceptable soil moisture content. Further studies are needed on the Model-II to make it acceptable in practical use.

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Development of Automatic Analysis System for Water Repellency of Packaging Paper -Automatic Measurement of Water Repellency Based on KS M 7057- (포장지의 발수도 자동 해석 시스템 개발 -KS M 7057에 근거한 발수도 자동 측정-)

  • 김철환;최경민;강진기;박종열
    • Journal of Korea Technical Association of The Pulp and Paper Industry
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    • v.35 no.1
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    • pp.7-12
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    • 2003
  • The test results for water repellency of paper and paperboard according to KS M 7057 can readily be influenced by an operator's bias. In order to discard such bias, the automatic analysis program of water repellency was developed based on different shape features of a liquid trace formed on a specimen. That is, the shape of a liquid track flowing down on the specimen with an angle of $45^{\circ}$ was evaluated according to width variation between head and tail of the trace($S_{Hr}$), uniformity of a liquid flow($S_{d}$), length of long traces($L_{SHr}$), eccentricity of liquid traces($E_{i}$), and then was recognized as a specific degree of water repellency. Finally, the automatic analysis system of water repellency based upon KS M 7057 made it possible to readily measure water resistance of paper and paperboards classified into R0 to R10.

A Study on the Quality Characteristics of Leather Shoe Adhesives(I) -Ladies' Leather-shoe- (구두용 접착제의 품질 특성에 관한 연구(I) -여성 가죽구두-)

  • 서교택;이상신
    • Journal of Korean Society of Industrial and Systems Engineering
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    • v.23 no.54
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    • pp.137-144
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    • 2000
  • The chloroprene adhesive for ladies' shoe-making applied by the cementing method in KS G 3116 has been evaluated.. For the quality test of adhesive for pump whole cut vamp using chloroprene adhesive, general property and adhesion strength were measured and duration for environmental exposure was tested. Also, a list of experiments for field application was prepared. In case of adhesion-strength measurement,, the KS G 3116 method of peel strength at end of toe was found to be reasonable by measurement through a whole length, toe to heel seat on feather edge.

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