• Journal of the Korean Society of Food Science and Nutrition
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• v.43 no.12
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• pp.1801-1807
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• 2014

Blue mussels (Mytilus edulis) are widely distributed among the world's oceans in various habitats. The purpose of this study was to investigate the effects of freeze-drying on the antioxidant and antigenotoxic activities of blue mussels collected in the Gyeongnam coast area of Korea. Raw (RM) and freeze-dried blue mussel flesh (FRM) were extracted with ethanol, methanol, and water. Antioxidant activities were evaluated on the basis of DPPH radical scavenging activity, oxygen radical absorbance capacity (ORAC), cellular antioxidant capacity (CAC), and antigenotoxic activity (comet assay). Except for the water extract, RM and FRM showed DPPH radical scavenging activities, which increased upon freeze-drying in MeOH extract. The highest ORAC value was observed in water extract of RM and MeOH extract of FRM. CAC was protected against AAPH-induced oxidative stress in HepG2 cells by both RM and FRM extracts. Freeze-drying lowered ORAC value of water extract, whereas it increased CAC activity, suggesting that antioxidant activities varied according to the generated radicals. All extracts from RM and FRM showed antigenotoxic activities by reducing $H_2O_2$-induced DNA damage in human leukocytes. Freeze-drying had no effect on antigenotoxicity of blue mussels. Taken together, these results indicate that blue mussels possess antioxidant and antigenotoxic properties, and freeze-drying might be a useful processing method for blue mussels to retain their maximum physiological potential as a functional food.

• Food Science and Preservation
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• v.5 no.4
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• pp.326-330
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• 1998

Freeze storage technique is widely used for food processing to keep freshness and quality of the product. This technique was applied to fresh, unshelled groundnut to develop a new type of product which could maintain fresh taste and nutritive values even after several months of storage. The groundnut varieties, Daepungtangkong, Daekwangtangkong and Sindaekwangtangkong were grown at the experimental field of NCES in 1996. Immatured pods or groundnut were harvested around 20 to 3o days before full maturity, washed, and steamed at 100$^{\circ}C$ for 5 min. to stop enzyme activity. After vacuum packing (at -760mmHg for 10 min.) with 0.08mm polyvinyldichloride film, the pods were immediately frozen at -70$^{\circ}C$ for 24h and transfered at -20$^{\circ}C$ for long-term storage. Physico-chemical properties of frozen vegetable groundnut were investigated at 2 months after storage and compared to those of conventionally dried groundnut. After 2 months storage, the thawed kernels were very palatable with softness and fresh taste. Acid value and hardness (measured as the compression force on the probe of a texture analyzer) were much lower in frozen vegetable groundnut than those in the air-dried ones. Presence of free sugars is one of the important factors affecting groundnut taste, and the free sugar contents were considerably decreased in the frozen vegetable groundnut compared to freshly harvested groundnut. But in dried groundnut no free sugar was detected.

• Journal of Life Science
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• v.21 no.1
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• pp.62-67
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• 2011

This study was performed to investigate the physiological activities of stings of Gleditsia sinensis extracts. Antioxidant activity was evaluated by measuring total phenolic contents (TPC), comet assay, and 2.2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity (RSA). Anti-diabetic activity was measured by inhibition activities on $\alpha$-glucosidase. Stings of Gleditsia sinensis extracts were prepared by extracting them with methanol and ethanol. The methanolic extracts showed the highest phenol content (1.12 g/100 g gallic acid equivalents). The $\alpha$-glucosidase inhibitory activity of methanol extracts were 17.9% higher, and that of ethanol extracts were 10.3% higher at a concentration of 1 mg/ml. These results indicate that stings of Gleditsia sinensis might be potential candidates as antioxidant and anti-diabetic agents.

• Journal of Pharmaceutical Investigation
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• v.29 no.3
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• pp.217-225
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• 1999

These studies were designed to determine the effect of hydroalcoholic gel system (lower alkanol concentration: 40-60%) compared to general hydrogel system (lower alkanol concentration: 10-35%) on transdermal delivery of piroxicam and its anti-inflammatory activity. Piroxicam was incorporated into a hydroalcoholic gel and a hydrogel containing polymers, solvents, and cosolvents. The pH of gel was about 6.3-7.3 and the solvent mixtures were composed of water and various concentrations of ethanol (35, 40, 50, and 60%). For the in vitro study, the skin permeation of piroxicam from the gel formulations was investigated using Franz modified diffusion cells fitted with hairless mouse skin. For the in vivo study, the anti-inflammatory activity of hydroalcoholic gel was compared to other commercial products (piroxicam hydrogel and ketoprofen hydrogel) in rat and human. The anti-inflammatory activity was determined using carrageenan induced foot edema model in rat. For the clinical study, it was evaluated from determining efficacy and acceptability with 98 patients suffering from musculoskeletal pain. A novel piroxicam hydroalcoholic gel was successfully formulated in the range of 40-50% of ethanol as solvent, more than 10% of propylene glycol, 5% of $Transcutol^{\circledR}$ and 1 % of benzyl alcohol. The skin permeation of piroxicam using hydroalcoholic gel system was greater than that of general hydrogel system $(flux\;:\;139.1-148.2\;{\mu}g/cm^2/hr\;vs.43.0-84.5 {\mu}g/cm^2/hr)$ in vitro. In carrageenan-induced edema model, the anti-inflammatory activity of hydroalcoholic gel was better than that of piroxicam hydrogel for edema inhibition (75.1 % vs. 62.9%, p

• Proceedings of the Korean Society for Agricultural Machinery Conference
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• 2002.02a
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• pp.457-463
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• 2002

이 연구는 x-선 투과영상을 이용하여 홍삼의 내공을 검출하기 위하여 수행되었다. 이를 위하여 x-선관에 조사되는 x-ray 양의 차이에 따른 위치별 밝기 값 차이를 보정하고 내공 검출에 적합한 영상처리 알고리즘을 개발하였다. 주요연구 결과를 요약하면 다음과 같다. 1) x-선관에 조사되는 x-ray양은 중심에서 가장자리로 갈수록 감소하며, 이것은 위치에 따른 gray 값의 분포가 달라지게 되는 원인이 되었다. 2) 홍삼을 2치화 하기 위해서는 동일한 x-선 조사강도에서 입력된 원 영상에서 빈 영상을 뺀 감산 영상을 만들어 줌으로써 가능하였다. 3) 조사강도 별 감산영상처리 결과 36kV/4.15mA와 39kV/4.15mA에서는 조사량이 많아서 홈삼 지근 부분의 영상이 손실되는 경우가 발생하였다. 4) 내공 검출을 위해서는 정상부분과 내공부분의 자기 값 차이를 크게 하는 전처리 영상을 만들 필요가 있었고, multiple 감산영상에서 뚜렷한 골짜기가 나타났으나 주근의 내공부분의 밝기값 보다 지근의 정상부분의 자기 값이 더 낮게 나타나 이를 보정하기 위하여 홍삼 위치에 따라 부분 보정된 알고리즘을 개발하였다. 5) x-선관의 놓인 위치에 따라서 내공판정시험 결과 중심에 있을 때에 비해 가장자리에 있을 때는 영상이 일부 손실되었으며, 중심 위치에서 서로 다른 굵기의 홍삼에 대해서는 모두 양호한 결과가 나타났다. 6) 완전한 홈삼으로 내공판정시험 결과 내공주위의 정상부분가지도 일부 내공으로 잘못 검출되었으나 이것을 재차 line profile에 의해 한 라인씩 문턱 값을 설정하여 내공만을 정확하게 판정하는 알고리즘을 개발하였다.양체의 접종작업은 모든 배양실이 인력에 의존하였으며, 배양체를 배지와 분리하여 불필요한 부분을 제거하고 배양작물에 따라 생육정도를 2~3등급으로 구분하여 배양용기의 배지 위에 치상하는 과정으로 수행되었으며, 작업능률은 호접란의 경우 배양병에 25본을 접종하는데 시간당 6병, 심비디움은 원형 플라스크에 25본을 접종하는데 시간당 10병 정도였다. 바. 식물체의 대량증식에 사용되는 플라스크, 배양병, PE용기 등 배양용기의 세척작업은 농원의 1개배양실에서 간이식 세척기, 이 외의 9개배양실은 모두 물에 담겨 두었다가 세제와 브러쉬 등을 사용하여 인력으로 세척하고 있어 생력화 기술개발이 요구되었다.도가 빠를수록 건조속도가 빨라졌으며, 건조에너지도 1,334kcal/kg.water로 비슷하게 소요되었다. 마. 시험구와 대비구의 건감률은 시험구에서 1.08~1.36w.b./h로 나타나 대비구보다 약 9.9~18.3%가 높게 나타났고, 건조에너지는 10.2~14.6%가 절감되었다. 발아율은 열풍온도가 낮을수록 높게 나타났고 시험구가 대비구보다 발아율이 낮게 나타났으며, 동할률 증가량도 원적외선.열풍 복합건조방법이 높게 나타나 이것은 곡물 표면에 원적외선 방사에의한 복사열이 전달되어 열장해를 받았기 때문으로 판단되며, 금후 더 연구하여 적정 열풍온도 및 방사체 크기를 구명해야 할 것이다.으로 보여진다 따라서 옻나무 유래 F는 포유동물의 생식기능에 중요하게 작용하는 것으로 사료된다.된다.정량 분석한 결과이다. 시편의 조성은 33.6 at% U, 66.4 at% O의 결과를 얻었다. 산화물 핵연료의 표면 관찰 및 정량 분석 시험시 시편 표면을 전도성

• Proceedings of the Korean Society for Agricultural Machinery Conference
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• 2002.02a
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• pp.438-443
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• 2002

본 연구는 수도작에서 토양과 식물체의 분광반사특성과 영상처리를 이용한 기계시각 잡초검출 센서를 개발하기 위한 기초연구로서 분류하고자 하는 대상체들의 분광반사율을 조사하여 주요한 파장을 선정하고 선정된 파장을 이용한 판별분석을 통해 각 대상체에 대한 분류 정확도를 중심으로 잡초검출 가능성을 조사하기 위하여 수행하였으며, 실험으로부터 얻은 결론은 다음과 같다. 1. 토양과 식물체를 구분하는데 효과적인 파장은 마른 토양의 경우 680 nm, 배수 토양에 있어서는 810 nm로 선정하였고, 토양을 배제한 후 벼와 잡초를 구분하기에 효과적인 파장은 580, 680 nm로 선정하였다. 2. 토양과 식물체를 구분하기 위한 판별분석 결과 2가지 토양상태 모두 식물체와 완전히 구분 가능한 것으로 나타났다. 벼와 잡초를 구분하기 위한 실험에서, 벼는 98%의 분류정확도로 구분이 가능하였고, 잡초는 83%의 분류정확도로 구분이 되는 것으로 나타났다. 따라서 차후 분광학적 원리를 이용한 센서를 제작할 때 본 연구에서 선택한 주요 파장과 판별함수를 이용하여 장치를 구성하고 알고리즘을 제작한다면 벼, 잡초, 토양을 효과적으로 구분이 가능할 것으로 판단되었다. 3. 컬러 CCD 카메라를 사용하는 경우에 있어 식물체와 토양을 구분하기 위해 3 종의 파장 중 630 nm 파장만의 이용을 고려하여 그 분류성능을 분석한 결과, 식물체와 토양은 소수의 관측치를 제외하고 완전히 구분이 가능했고, 벼와 잡초를 구분한 결과에서는 비교적 높은 분류능력을 가진 것으로 나타나 차후 컬러 CCD 카메라를 이용하여 장치를 구성하는데 좋은 기초가 될 것으로 판단된다. 배양체의 접종작업은 모든 배양실이 인력에 의존하였으며, 배양체를 배지와 분리하여 불필요한 부분을 제거하고 배양작물에 따라 생육정도를 2~3등급으로 구분하여 배양용기의 배지 위에 치상하는 과정으로 수행되었으며, 작업능률은 호접란의 경우 배양병에 25본을 접종하는데 시간당 6병, 심비디움은 원형 플라스크에 25본을 접종하는데 시간당 10병 정도였다. 바. 식물체의 대량증식에 사용되는 플라스크, 배양병, PE용기 등 배양용기의 세척작업은 농원의 1개배양실에서 간이식 세척기, 이 외의 9개배양실은 모두 물에 담겨 두었다가 세제와 브러쉬 등을 사용하여 인력으로 세척하고 있어 생력화 기술개발이 요구되었다.도가 빠를수록 건조속도가 빨라졌으며, 건조에너지도 1,334kcal/kg.water로 비슷하게 소요되었다. 마. 시험구와 대비구의 건감률은 시험구에서 1.08~1.36w.b./h로 나타나 대비구보다 약 9.9~18.3%가 높게 나타났고, 건조에너지는 10.2~14.6%가 절감되었다. 발아율은 열풍온도가 낮을수록 높게 나타났고 시험구가 대비구보다 발아율이 낮게 나타났으며, 동할률 증가량도 원적외선.열풍 복합건조방법이 높게 나타나 이것은 곡물 표면에 원적외선 방사에의한 복사열이 전달되어 열장해를 받았기 때문으로 판단되며, 금후 더 연구하여 적정 열풍온도 및 방사체 크기를 구명해야 할 것이다.으로 보여진다 따라서 옻나무 유래 F는 포유동물의 생식기능에 중요하게 작용하는 것으로 사료된다.된다.정량 분석한 결과이다. 시편의 조성은 33.6 at% U, 66.4 at% O의 결과를 얻었다. 산화물 핵연료의 표면 관찰 및 정량 분석 시험시 시편 표면을

• The Korean Journal of Pharmacology
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• v.31 no.2
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• pp.195-206
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• 1995

The study was undertaken to examine the possibility of the involvement of $K^+$ channels in the mechanism of relaxant-action of imipramine on the isolated canine detrusor muscle strips. Canine urinary bladder were isolated, and smooth muscle strips of 15 mm long and 2 mm wide from the mid-portion of anterior wall were made in the Tyrode solution of $0{\sim}4^{\circ}C$. The strips were prepared for isometric myography in Biancani's isolated muscle chamber containing 1 ml of Tyrode solution, which was maintained with pH 7.4 by aeration with $95%\;O_2/5%CO_2\;at\;37^{\circ}C$. RP 52891, a non-specific $K^+$ channel opener, concentration-dependently suppressed the spontaneous phasic contractions of the detrusor strips. Imipramine, a tricyclic antidepressant, also reduced the spontaneous contractions in a concentration-dependent manner. RP 52891 was more potent than imipramine(p<0.05), and Imipramine was more efficient than RP 52891(p<0.05).Procaine, a voltage-dependent $K^+$ channel blocker, glibenclamide, an ATP-dependent $K^+$ channel blocker, and apamin, a calcium-dependent $K^+$ channel blocker antagonized the relaxant effect of RP 52891, but not of imipramine. Imipramine reduced the electric field stimulation (EFS) -induced contractions concentration-dependently. None of the $K^+$ channel blockers employed for this study, procaine, glibenclamide or apamin antagonized the inhibitory action of imipramine on the EFS-induced contraction. These results suggest that in canine detrusor, the $K^+$ channels of the characteristics of voltage-dependent, ATP-dependent and/or calcium-dependent are exist, and the inhibitory action of imipramine on the contractility of the detrusor is independent from the $K^+$ channels.

• Applied Biological Chemistry
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• v.49 no.3
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• pp.215-220
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• 2006

Response surface methodology was implemented to determine an optimal extraction condition in Phellinus linteus water extract. Extraction was performed on 10 experimental conditions including independent variables such as extraction time $(1{\sim}5\;hrs)$ and water volume over sample (sample : $H_2O$ = 1 : $40{\sim}200$, W/V), color browning, reducing and total sugar, that were based on the significant levels of 10% of central composition design. Color browning, reducing and total sugar contents were found to be more affected when the water volume was increased rather than extraction time. Maximum extraction condition was acquired at extraction time of $3.0{\sim}4.5\;hrs$ and water volume of $40{\sim}58.2\;ml$. Being extracted at the optimal extraction condition two of the free sugars, sucrose (0.126%) and glucose (0.012%), were detected. Total content of the free amino acids was found to be $503.26\;{\mu}g%$. Among them, essential amino acid contents were revealed as 5.4%. One major peak from gel permeation chromatography contained polysaccharide(s) with the molecular weights of 10 KDa.

• Restorative Dentistry and Endodontics
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• v.25 no.1
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• pp.144-152
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• 2000

Intracoronal bleaching is currently disregarded by many clinicians because of the potential consequence of cervical resorption. To prevent this complication it is recommended that intra coronal barrier materials be placed over the root canal obturation and sodium perborate be used with water rather than with hydrogen peroxide. The purpose of this study was to evaluate the amount of the hydrogen peroxide penetration according to the difference in intracanal base materials and sodium perborate preparation. Fifty extracted intact premolars were instrumented, and filled with gutta-percha. And then the outer surface of the teeth was sealed with wax exposing the CEJ. The prepared teeth were placed in plastic tubes containing 1.5ml distilled water with their entire root submerged into the solution, The teeth were divided into the following five groups. In the first two groups gutta-percha was removed without placement of barrier, and then water or superoxole(30% $H_2O_2$) with sodium perborate were used respectively for bleaching. In the other three groups, after removal of gutta-percha, an intracanal isolating barrier(ZPC, IRM, Fuji II LC) was placed and then bleached with sodium perborate and superoxole. The bleaching procedure was performed 4 times with 1 week interval. The results were as follows : 1. All the groups showed a tendency of increasing penetration amount with increasing treatment times(P<0.05). 2. After the 1st and 2nd treatments, there was no significant difference in microleakage among the groups. 3. After the 3rd bleaching with superoxole and sodium perborate, there was no significant difference in microleakage between gutta-percha alone group and gutta-percha with ZPC, Fuji II LC barrier group. But significant difference was found between IRM barrier group and other groups(P<0.01). 4. After the 4th bleaching with superoxole and sodium perborate, there was no significant difference between gutta-percha alone group and gutta-percha with barrier groups. 5. After the 4th treatment, the group bleached with sodium perborate and water without barrier showed lower hydrogen peroxide penetration than that of other groups(P<0.01).

• Korean Journal of Food Science and Technology
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• v.48 no.1
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• pp.36-41
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• 2016

In this study, the effects of intense pulsed light (IPL) treatment on microbial inactivation and quality in rapeseed sprouts were investigated. Untreated rapeseed sprouts exhibit a high level of total aerobic bacteria (TAB) ($1.2{\times}10^7CFU/g$), coliform bacteria (coliform) ($3.3{\times}10^6CFU/g$), and pathogenic E. coli (PE) ($2.1{\times}10^5CFU/g$). The microorganisms found on rapeseed sprouts decreased with exposure to increasing light intensity and treatment time. The greatest reduction in microbial content was observed with a treatment of 1000 V, 5 pps for 10 min, where TAB, coliform, and PE levels decreased to 1.0 log CFU/g, 1.6 log CFU/g, and 1.8 log CFU/g, respectively. In agreement with these data, the microbial inactivation rate increased with the increase in the distance between the light source and the samples during IPL treatment. After IPL treatment of rapeseed sprouts, water content and vitamin C content decreased.