• Journal of the Korean Chemical Society
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• v.63 no.2
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• pp.85-93
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• 2019

A panel of chalcone-sulphonamide hybrids has been designed by tethering appropriate sulphonamide scaffold with substituted chalcones as a multi-target drug for anticancer screening. Chalcones were prepared by Claisen-Schmidt condensation reaction of a substituted aldehyde with para aminoacetophenone. All the synthesized compounds were evaluated against selected five cancer cell lines, MCF-7 (Breast cancer), DU-145 (Human prostate Carcinoma), HCT-15 (Colon cancer), NCIH-522 (stage 2, adenocarcinoma; non-small cell lung cancer) and HT-3 (Human cervical cancer). Most of the synthesized chalcone-sulphonamide hybrids showed amended cytotoxic activity against various cancer cell lines which may be attributed to the linkage of sulphonamide with chalcone skeleton. The synthesized compounds were characterized by FT-IR, $^1H$ NMR, $^{13}C$ NMR and HR-LCMS and spectral study assert the structures of synthesized sulphonamide-chalcone hybrids.

• Journal of Korean Medicine Rehabilitation
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• v.19 no.1
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• pp.145-154
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• 2009

Objectives : The aim of this study was to investigate the characteristic of ryodoraku in the chronic low back pain patients who visited department of oriental rehabilitation medicine. Methods : Subjects were 32 patients who visited department of oriental rehabilitation medicine(<60 years old). The low back pain patients were measured points of twelve meridians by ryodoraku diagnosis and checked the duration of low back pain. We compared the mean ryodoraku and the percentage of each points beyond the physiological range. Results : The score of H1(LU9), H2(PC7), H3(HT7), H5(TE4) and F5(GB40) had statistical differences compared with mean ryodoraku. And the correlation between duration of pain and mean ryodoraku showed statistically significant negative correlation(r=-0.353). Conclusions : These results suggest that ryodoraku maybe used to evaluate chronic low back pain patients.

• Korean Journal of Oriental Medicine
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• v.11 no.2
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• pp.113-140
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• 2005

1. 백렴 추출물은 부분적으로 종양세포인 HT1080, Hep G2, MCF-7 cell에 대해 세포독성을 보였으나, 섬유세포인 7250 cells과 종양세포인 CT-26 cells에서는 세포독성은 나타나지 않았다. 2. 백렴 추출물은 macrophage에서 생산되는 NO production을 부분적으로 증가시켰다. 3. 백렴 추출물은 macrophage 활성화와 관련 있는 IL-1과 iNOS의 유전자 발현을 증가시켰다. 4. 백렴 추출물은 NK cell의 cytotoxicity를 활성화시켰다. 5. 백렴 추출물은 NK cell 활성화와 관련있는 IL-1, IL-4, IL-10, IL-12, iNOS, IFN-${\gamma}$, TNF-${\alpha}$ 의 유전자 발현을 증가시켰다. 6. 백렴 추출물은 마우스의 비장세포에서 IL-10, IFN-${\gamma}$, TNF-${\alpha}$ 의 단백질 발현을 증가시켰다. 7. 백렴 추출물은 CT-26 cell에 의한 pulmonary colony를 대조군에 비해 유의성 있게 억제시켰다. 이상의 결과로 백렴 추출물은 macrophage와 NK cell의 활성화를 통해 면역 조절과 항암제로서의 유용성이 기대된다.

• Journal of Nutrition and Health
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• v.16 no.3
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• pp.216-227
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• 1983

As an attempt to improve the nutritional status of Korean rural pre-school children, anthropometry, clinical examination, and hematological parameters(Hb, Ht, and serum albumin) are measured. Six kinds of diet supplemented with 0, 2.5, 5.0, 7.5, 10.0 and 20.0 mg Fe(as ferrous sulfate) were used for 10 weeks. Subjects were 349 pre- school children aged from 1 to 5 years old living in An-gam-my-on and Bong-dang-myon, Hwa-seung-goo, Kyong-gi-duo, and the supplementary diet was a product of the Model Nutritional Enterprise Factory in Wuen-sueng-goon, Gang-won- duo. The results obtained were as follows : 1) Physical development of subject children were within normal range during the feeding trial period of 9 weeks. 2) From the results of clinical examination, no children showed nutritional deficiency signs. 3) Iron free supplementary diet group( control group ) among other subject children also showed some improvement of hemoglobin level ana frequency of anemia. 4) The amount of iron fortified as supplemental diet was effective in 5.0-7.5 mg Fe per child per day, no further effect was observed by adding of more iron. 5) The values of hematocrit and serum albumin also improved by providing the supplementary diet.

• Proceedings of the Korean Society of Applied Pharmacology
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• 2003.11a
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• pp.102-102
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• 2003

The inhibitors of prostaglandin biosynthesis and nitric oxide production by corresponding inducible isozyme have been considered as potential anti-inflammatory and cancer chemopreventive agents. In our continuous search for cancer chemopreventive agents from natural products, we have evaluated the inhibitory potential of PGE$_2$and NO production in lipopolysaccharide (LPS)-induced mouse macrophage RAW264.7 cells. As a result, pinosylvin (3,5-dihydroxy-trans-stilbene), a stilbenoid, mainly found from the heartwood and leaves of the Pinus sylvestris, showed potential inhibitory activity of LPS-induced PGE$_2$and NO production in a dose-dependent manner. Pinosylvin also suppressed the LPS-induced iNOS protein expression. Further study revealed that pinosylvin exhibited antioxidant activity by the DPPH free radical scavenging potential and inhibitory effect of xanthine oxidase activity. In addition, pinosylvin inhibited COX -2 overexpressed human colon cancer cell (HT-29) growth in a time- and dose-dependent manner. These findings suggest that pinosylvin might be a promising candidate for developing cancer chemopreventive agent.

• Journal of the Korean Society of Physical Medicine
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• v.7 no.4
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• pp.451-458
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• 2012

PURPOSE: The purpose of present study was to determine effects of circuit class training (CCT) on the synthesis of central serotonin in people with post-stroke depression. METHODS: Forty patients with mild post-stroke depression were participated in current study. All subjects were assigned to two groups of CCT (circuit class training) group and SW (stretching and weight shifting) group. Both groups were performed for 80 minutes. The change of blood f-Trp, BCAAs, f-Trp/BCAAs and serotonin according to period training time were examined and the following results were obtained. RESULTS: In the CCT group, f-Trp, BCAAs, and f-Trp/BCAAs increased according to the time point. However, after 24 hour of circuit class training, all of these were significantly decreased by those before training. The change in blood levels of serotonin was the highest in immediately after the training in the CCT group while it was not changed significantly in the SW group. CONCLUSION: It can be seen that CCT can change the serotonin level and have an effect on the synthesis of central serotonin in people with post-stroke depression.

• Parasites, Hosts and Diseases
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• v.37 no.4
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• pp.285-287
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• 1999

Changes in the expression level of splenocyte $IFN-{\gamma}$mRNA of Sprague-Dawley (SD) rats infected with Paragonimus westermani were analyzed by competitive reverse transcription-polymerase chain reaction (RT-PCR) followed by southern blot. The template RNA was extracted from the splenocytes of rats infected with 20 metacercariae of P. westermani. The products of competitive RT-PCR were subjected to southern blot and enhanced chemiluminescence (ECL), and analyzed with a densitometer. In comparison with that of uninfected control rat splenocytes (value of 1), the levels of mRNA expression of $IFN-{\gamma}$had changed to 0.747 at 1 week post infection (PI), 0.00175 at 2 week PI, 0.0217 at 3 week PI, 0.194 at 4 week PI and then to 0.537 at 5 week PI. The level at 7 week PI had returned to 1.25, comparable with that of uninfected rats. These results show that, when infected with p. westermani, the levels of $IFN-{\gamma}$ mRNA of SD rat splenocytes were remarkably reduced by more than 500 times at 2 week PI and restored to normal level at 7 week PI.

• Korean Journal of Animal Reproduction
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• v.18 no.3
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• pp.157-165
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• 1994

This experiment was examined the effect of splenectomy on the hematology in pregnant wistar rat. Only animals that had been shown regular 4-day estrous cycles for more than two cycles were used. The day after mating with the same male animal ws designated Day 0 of pregnancy. Spleen was removed from Day 0(early), 6(middle) and 13(late) of pregnant rat, respectively. Blood sample was collected at Day 1, 7, 14 and 21 of the pregnancy. 1. RBC was increased significantly(P<0.05) to the progress of pregnancy in control rat. The late splenectomized rats were decreased significantly(P<0.05) at Day 21 of pregnancy than control rats. 2. Hb was increased significantly (P<0.05) at 21th day of pregnancy in late splenectomized groups than others group. 3. In the late splenectomized rats, Ht was decreased significant (P<0.05) due to the progress of pregnancy and decreased significantly (P<0.05) at Day 21 of pregnancy in all splenectomized groups. 4. WBC was increased significantly (P<0.05) at Day 1 of pregnancy in splenectomized groups compared with control. 5. In differential leukocyte rate, the Basophils and Monocytes was not significantly changed. Neutrophils was increased significantly(P<0.05) at Day 14 and 21 than Day 1 and 7 of pregnancy in control. Lymphocytes was decreased significantly(P<0.05) in control due to progress of pregnancy. Neutrophils was increased and Lymphocytes was decreased significantly(P<0.05) in splenectomized groups compared with control.

• Korean Journal of Applied Biomechanics
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• v.23 no.1
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• pp.1-9
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• 2013

The purpose of this study was to provide information on athletes' characteristics and advantages and disadvantages by analyzing the kinematic factors having a decisive influence on competitive abilities such as release conditions in the delivery phase on major Korean javelin athletes. Two supported javelin throwers of the Korean national team participated in this study. The total average time required of the delivery phase was 0.31(${\pm}0.016$). The athletes' release angle was 33.2 to 41.7 degrees. The attack angle varied widely from -3.5 to 5.9 degrees. The Javelin heights of Subject A and B were 95.9 and 89.2%Ht. The average stride length were 180.6 and 176.7cm. The center of mass velocity of LFD and REL was relatively low in all the subjects. The average deceleration rates of center of mass velocity of Subject A and B were 57.2 and 48.9%lose. The left knee angles of Subject A and B were 160.1 and 155.5 degrees in LFD, 153.0 and 164.0 degrees in REL. The joint velocity of upper limb segments was relatively low in all the subjects. The maximum average wrist velocity of Subject A and B was 18.2 and 16.3 m/s in REL.

• Journal of Microbiology and Biotechnology
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• v.26 no.11
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• pp.2006-2011
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• 2016

The marine sponges Hyrtios and Haliclona species, both of which are known to produce secondary bioactive metabolites, were used to extract 1304KO-327 and 1304KO-328. Such secondary metabolites are potentially antibacterial, antiviral, anti-inflammatory, antitumoral, antifungal, and antiplasmodial. In the present study, the effects of 1304KO-327 and 1304KO-328 were studied for their clinical and pathological importance. The cytotoxicity of 1304KO-327 and 1304KO-328 was assessed via MTT(3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay on HT-29, Caco-2, and Raw 264.7 cells. Rotavirus-infected Caco-2 cells were used to prove the antiviral effects of the marine sponge extracts. The test results cogently proved that the virus-inhibiting effects of the sponge extracts improved with extract concentration. Anti-inflammatory effects of the marine sponge extracts were tested on Lipopolysaccharide-treated Raw 264.7 cells. Nitric oxide and cytokine were produced by treatment of the cells with LPS and the inhibiting effects of the sponge extracts on $IL-1{\beta}$ formation were investigated. This study found that the NO production was decreased dose dependently, and $IL-1{\beta}$ formation was significantly reduced by the marine sponge extracts.