• Horticultural Science & Technology
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• v.33 no.1
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• pp.106-113
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• 2015

Effects of mineral salts (N, P, K, $Ca^{2+}$, $Mg^{2+}$, and $Fe^{3+}$) on the shoot growth and metabolite production of tea tree were studied using in vitro culture techniques. Among mineral s alts, ${H_2PO_4}^-$ was the most important for enhanced growth rate of tea tree, while $Mg^{2+}$ and $Ca^{2+}$ did not affect plant growth. Removal of ${NH_4}^+$ and $NO_3$ from the culture medium enhanced shoot multiplication compared to other treatments. Metabolite production was variable depending on mineral types and concentration. Removal of $Ca^{2+}$ decreased the production of caffeine; however, other treatments did not influence its production. $Ca^{2+}$, ${NH_4}^+$ and $Fe^{3+}$ were important factors for catechin production in tea tree. These results can be used as the basis for development of technical soil controls suitable for tea tree cultivation in the future.

• Proceedings of the Korean Society for Applied Microbiology Conference
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• 1978.04a
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• pp.96.3-96
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• 1978

Biotin 요구성 glutamic acid 생산능을 가진 Br-evibacterium sp.의 isoleucine 영양요구성 변이주가 다량의 L-leucine을 생성함을 발견하였다. 본균주에 관한 탄소원, 질소원, 무기염, vitamin, amino acid 등의 영양물질과 온도, 공기주입량, pH등에 관한 실험으로 다음과 같은 결과를 얻었다. 본균주는 탄소원, 질소원으로서 10% glucose와 4% $(NH_4)_2SO_4를$ 공합하여 $30^{\circ}C,$ 88시간 배양시킨 결과, 액중에 21 mg/ml의 L-leucine이 축적되었다. Fe$^{2+}$와 $Mn^{2+}$의 동시첨가는 L-leucine생성에 효과적이었으며 thiamine 역시 L-leucine 생성에 효과적이었다. 30ι jar fermentor 에서 폐당밀을 주원료로 한 실험결과, 폐당밀, $(NH_4)/SO_4,$ $KH_2PO_4,$ $MgSO_4ㆍ7H_2O로$ 구성된 액체배지에서 $30^{\circ}C,$ 36시간 배양시킨 것이, 27mg/ml의 L-leucine이 축적되었으며 이때의 대당수율은 16%이었다.

• Microbiology and Biotechnology Letters
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• v.17 no.6
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• pp.593-599
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• 1989

A strain producing extracellular endo-type inulase was selected from Actinomycetes isolated from soil, and identified as Streptomyces sp. The maximum inulase production was obtained with medium containing inulin 1.0%, yeast extract 1.0%, (NH$_4$)$_2$HPO$_4$ 0.4%, NH$_4$H$_2$PO$_4$0.8%, KCl 0.05%, MgSO$_4$ㆍ7$H_2O$ 0.05%, FeSO$_4$ㆍ7$H_2O$ 0.001% at 96 hours culture in jar fermentor. The endo-type inulase was considered to be an inducible enzyme produced by inulin only.

• The Korean Journal of Food And Nutrition
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• v.12 no.5
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• pp.470-477
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• 1999

The purpose of this study was to choose prinicipal food components contained in diet foods and food additives used for manufacturing processed foods and elucidate their in vivo effects on the growth pattern of Helicobacter pylori. To do this the antibacterial effects of various sources of carbon nitrogen and mineral as an effect agent on Helicobacter pylori were first assessed based upon bacterial growth degree. results show that the source of carbon tested had different effects on bacterial growth of Helicobacter pylori. It was revealed that a promotional effect of monosaccharides resulted in enhanced growth of Helicobacter pylori compared with disaccharides and polysacchrides, in particular glucose was observed to be most effective in growth of Helicobacter pylori among monosaccharides teste whereas mannose to hinder the growth of Helicobacter pylori. Polyols such as sorbitol mannitol maltitol and xylitol was however observed to show no promotion or suppression effect on growth of Helicobacter pylori. Apart from these the sources of amino acid and inorganic nitrogen were chosen and tested to assess the promotion or suppression effect of nitrogen sources on growth of helicobacter pylori. It was found that amino acid such as lysine showed its promotion effect on the growth of Helicobacter pylori while arginine (NH4)2SO4 and NH4Cl showed no effect on its growth. Ammoia and urea were however observed to have a positive effect on the growth of Helicobacter pylori. Among these effect agents lysine and methionine were revealed to show the most positive effect on growth of Helicobacter pylori. Minerals such as MgSO4 KH2PO4 and MgCl2 appered to exert their positive growth effects whereas CaCl2 and CaSo4 had a little effect. In addition FeSO4 FeCl2 and FeCl3 brought suppression on the growth of helicobacter pylori. In studies of the growth of Helicobacter pylori by food additives ascorbic acid showed extreme suppression on its growth,. Sodium nitrate and sodium chloride were also found to be of negative effect on the growth of Helicobacter pylori in rder of degree whereas tocopherol had nothing to do with microbial growth.

• Journal of the Korea institute for structural maintenance and inspection
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• v.20 no.2
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• pp.60-66
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• 2016

Polyurethane foam index a of the cell structure and the absorption change by using the foam stabilizer of six to investigate the polyurethane foam index producing the agent to the siloxane analyzed with silicon foam stabilizer with FE-SEM in accordance with the characteristics of the silicon-based foam stabilizer cell structure of the primary DC-193 on the chain ends is PO n dog bond, DC-2585, DC-5125, DC-198 has been confirmed as a close cell, silicone surfactant is combined EO n dog to a siloxane main chain terminus DC-5043 and DC-5598 that appeared to open cell structure. In addition, most absorption of the DC-5043 appeared was the size of the open cell greatest formed by the absorption of the cell structure change this absorption of the size of the close cell most detailed and uniform DC-193 appeared small household water-resistant best many showed. The performance test of the water was found to be excellent.

• Resources Recycling
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• v.7 no.2
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• pp.39-45
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• 1998

We smelted thc pellets made by mixing the distilled carbon from wlISte Lires, LD converter dust and slag with reduction process in the revcrberatory furnace. Thc obtained results are as follows 1) The removal mte of zinc appears above 97% after T reducing the pellets at $1300^{\circ}C$ for Ihr and the zinc content in the residue are 0.1~D.2%. 2) Under the mixing condition of 500 g LD dust. 150-200 g LD slag and 30-50 g distilled carbon of waste lires the removal raho of zinc shows above 95%, while t the 50-60% Fe remains in the residue. 3) After smelting at $1350^{\circ}C$ for 3hrs, the recovery ratio of pig iron reduced from lhe p pellets containing 15-20% LD slag and 4.1-7.2% distilled carbon of waste tires appears in the range of 89.3-92%. 4) Tbe c chemical composition of the recovered pig iron is 1.7%C, O.05%P, 0.05%S and balance Fe. 5) Tbe recovered dust from the d dust collcctor alter finishing the reduction rcaction appears as a crude zinc oxide conLaining 60% zinc.

• Korean Journal of Food Science and Technology
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• v.4 no.2
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• pp.123-133
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• 1972

Over 90 of lysine producing auxotrophs were obtained from Corynebacterium sp. S-27-12, Brevibacterium flavum ATCC 15168 and Micrococcus glutamicus ATCC 13032 by UV light, $Co^{60}$ irradiation and N-methyl-N'-nitro-N-nitrosoguanidine treatment. One of the mutant, Brev. flavum U46-N59, was identified as a leucine auxotroph and accumulated lysine during flask (500 ml) cultivation (180 strokes/min.) up to 21.6 mg per ml of broth at pH 7.5 and $28^{\circ}C$ after 4 days. The medium consisted of glucose, 100; urea, 10; corn steep liquor, 40; $KH_2PO_4,\;2;\;K_2HPO_4,\;0.5;\; MgSO_4.\;7H_2O,\;0.4;\;antifoam\;S-57,\;1g;\;Fe_2(SO_4)_3.XH-2O,\;10;\; MnCl_2,\;4H_2O,\;10mg;\;biotin,\;30;\;thiamine-HCl,\;100{\mu}g$in 1l of distilled water, and 40 U/ml of penicillin was added after 36 hrs fermentation.

• Current Research on Agriculture and Life Sciences
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• v.16
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• pp.45-54
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• 1998

A thermotolerant bacterium, Streptomyces thermocyaneoviolaceus which produced xylan-degrading enzymes, utilized excellently xylan of wheat bran by producing the enzymes in comparison with that of birchwood or oat spelts. Optimal enzyme production was achieved in WB medium containing 0.8% wheat bran, 0.06% yeast extract, 0.06% bactopeptone, 0.05% $MgSO_4{\cdot}7H_2O$, 0.05% $FeSO_4{\cdot}7H_2O$, 0.05% $KH_2PO_4$ and, 0.2% $K_2HPO_4$(pH 7.0) at $50^{\circ}C$ for 24 hrs. The optimal pH and temperature for the hydrolysis of xylan were pH 5.5 and $65^{\circ}C$, respectively. The enzyme activity was retained more than 80% at the range from pH 4.5 to pH 9.5 at $4^{\circ}C$ for 12 hrs and 94% on the heat-treatment at $65^{\circ}C$ for 1 hr. Xylobiose, xylotriose, xylose, and other xylooligosaccharides were produced as end products from hydrolysis of birchwood xylan by the xylanase of S. thermocyaneoviolceus.

• The Korean Journal of Mycology
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• v.25 no.4 s.83
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• pp.369-376
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• 1997

These researches were carried out for improvement of medium for mycelial growth of Hericium erinaceus isolate KU-1. It grew well at pH 4 and $25^{\circ}C$. Glucose and sucrose were favorable carbon sources for mycelial growth. As nitrogen sources, ammonium acetate and arginine enhanced mycelial growth. Optimum C/N ratio was 200. Based On the results, the following recipe is suggested for synthetic medium for the mycelial growth: glucose 18.02 g, arginine 2.613 g, ammonium acetate 2.313 g, $CaCl_2\;0.33\;g$, $KH_2PO_4\;8.5\;g$, $MgSO_4{\cdot}7H_2O\;2.0\;g$, $FeSO_4{\cdot}7H_2O\;0.02\;g$, $ZnSO_4{cdot}7H_2O\;0.02\;g$, $MnSO_4{\cdot}7H_2O\;0.02\;g$, water 1 liter. This medium was superior for the mycelial growth to other conventional media such as Yeast malt extract agar (YMA), Park medium, Potato dextrose agar (PDA), Malt extract agar, Czapek-dox agar, Macaya-lizano medium and Yeast extract agar. This new synthetic medium is designated as Ko medium.

• Journal of Life Science
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• v.13 no.6
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• pp.917-923
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• 2003

The present investigation was conducted to determine the chemical oxygen demand (COD) removal efficiency by Pseudomonas aeruginosa EMS1. The COD removal efficiency in the medium containing 1% metal working fluid (MWF) was 12% after cultivation of 4 days. The composition of optimum medium for the COD removal efficiency of 1% MWF by P. aeruginosa EMS1 were NH$_4$Cl 0.3%,$ K_2HPO_4\; 0.05%,\; KH_2PO_4\; 0.04%,\; MgSO_4.7H_2O\; 0.05%,\; CaCl_2.2H_2O 0.03%$ and $FeSO_4.7H_2O$ 0.04% at initial pH 7.0 and $30^{\circ}C$. Under this condition, the highest the COD removal efficiency was observed after 4 days.