• Journal of Plant Biotechnology
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• v.30 no.4
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• pp.353-357
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• 2003

We studies seed set in the interspecific F1 /backcross hybrids of Lilium species. In the interspecfic hybrid of L. longiflorum cv. Gelria with L.${\times}$ fomolongi cv. Raizan,93％ fruit set was obtained by stigmatic pollination in comparison to 53％ from cut-style pollination. Accordingly, the number of seed set resulting from stigmatic and cut-style pollination was 147 and 53, respectively. Pollination o( both stigmatic and cut-style pollination resulted in 47％ fruit set in the hybrid of L. longiflorum cv. Lorina with L.${\times}$ fomolongi cv. Raizan. However, stigmatic pollination formed 413 seeds, whereas only 24 seeds were obtained by cut-style pollination in this cross. The hybrid of L.${\times}$ fomolongi cv. Raizan with L. longiflorum cv. Come set 40％ fruit with a total of 43 seeds by stigmatic pollination. However, no fruit set was observed in cut-style pollination in this hybrid. Backcrossing the F1 hybrid by cut-style pollination of L. longiflorum cv. Lorina ${\times}$ Asiatic hybrid cv. Chicago with the latter parent led to 53％ fruit set, and 109 embryos were obtained. Likewise, backcrossing following cut-style pollination of L. longiflorum cv. Lorina ${\times}$ Asiatic hybrid cv. Corsia with the latter parent formed 67％ fruits and 107 embryos. However, in the remaining interspecific hybrids, cut-style pollination set no fruit.

• Plant Resources
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• v.5 no.1
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• pp.1-6
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• 2002

Genetic improvement of the cultivated soybean [Glycine max (L.) Merr] may be possible through hybridization with its wild progenitor, G. soja Sieb. & Zucc. Interspecific cross between G. max (Hwangkeumkong) and G. soja (IT.182932) was made in the summer of 1997. In F$_2$ the percentage of plant height, nodes per plant, and pods per plant were high but gradually reduced from F$_2$ to F$_4$. In contrast pod length, seeds per pod, and 100-seeds weight were increased gradually through generations advanced. Wild variation as evident in F$_2$ in plant height, number of branches, pods per plant, and 100-seeds weight. Twenty six percent of the F$_2$, 44 % of the F$_3$ and 60% of the F$_4$ segregants showed more G. max traits. The combination of useful traits from both species is possible through interspecific hybridization. The characters that could be transferred from wild species to cultivated species are more pod number, better capacity, and resistance to disease and insects. The interspecific derivatives offer scope for selection for high grain yield. Therefore, introducing genes from G. soja to G. max could be contribute to greater genetic diversity of future cultivars. And semicultivated soybean had some desired characteristics including tolerance to adverse environments and multi-seed characters. It means the infusing of semicultivated germplasm to the cultivated soybean could increase number of seeds and pods per plant significantly, and consequently could enhance selecting potential on yield.

• Journal of agriculture & life science
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• v.52 no.6
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• pp.27-36
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• 2018

Recently, the demand for new cultivar of oriental mustard(Brassica juncea) is increased as the consumption of oriental mustard has increased dramatically in the market due to Kimchi is attracting the world's attention. However, absence of seed supply system can cause many problems including inbreeding depression due to self seed production, deterioration of the seed purity and heterogeneity of commercial seed. To establish the $F_1$ variety breeding system of oriental mustard, pure line and inbred lines were screened from inbreeding genetic resources. Male-sterile line was also selected from breeding combinations for the quality improvement of mustard. The combining ability from(Indojasai ${\times}$ Goheungdamyang) combinations and isolation line of(MS910 ${\times}$ Japan red mustard 8 ${\times}$ Ganghwa mustard 9) was highest, thus these lines were selected as parental lines. PCR(Polymerase Chain Reaction) and gene sequence analysis revealed that the genes related to CMS(orf263, orf220, and orf288) were distributed in mitochondria. The isolated lines from this study also showed good performance in yield test and farmhouse prove test.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.57 no.2
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• pp.188-194
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• 2012

This study was carried out to evaluate the quality properties, fatty acid composition and amino acid composition of major Korean inbred lines and hybrids of maize for corn breeding. Protein content of $F_2$ seeds was 9.0%, which was slightly lower than those of inbred lines, 11.4%, and $F_1$ seeds, 10.9%. in hybrid maizes. Lipid content of $F_2$ seeds of hybrid maizes was 4.2%, slightly higher than those of inbred lines, 3.3%, and $F_1$ seeds, 3.6%. However, there was no significant difference in ash content. The amount of linoleic acid in inbred lines, $F_1$ and $F_2$ seeds of hybrid maizes was highest among all fatty acids and followed by oleic acid, palmitic acid, stearic acid and linolenic acid. The composition of saturated fatty acid of $F_2$ seeds was 17.6%, similar to $F_1$ seeds but it was lower than that of inbred lines. The composition of unsaturated fatty acid of $F_2$ seeds was 82.4%, similar to $F_1$ seeds but was higher than inbred lines. The content of leucine of $F_2$ seeds was lower than inbred lines and $F_1$ seeds. The content of valine, serine, threonine, cysteine, aspartic acid, lysine, arginine, histidine of $F_2$ seeds was higher than inbred lines and $F_1$ seeds. MMA (monoamino monocarboxylic acid) in inbred lines, $F_1$ and $F_2$ seeds of hybrid maizes was highest among all amino acids and followed by DMA (diamino monocarboxylic acid) and AAA (aromatic amino acid).

• Korean Journal of Breeding Science
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• v.41 no.4
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• pp.664-668
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• 2009

"Boram" is a new $F_1$ hybrid of rapeseed (Brassica napus L.) with early maturing, high yield and high oleic acid. This cultivar was developed from the cross between Mokpo-CGMS (male steile line) and 8634-B-1-3-1-8 (restore line) for the production of edible oil and biodiesel in 2006. Yield trials were conducted from 2007 to 2008 and regional adaptation trials were examined at four locations in 2008. "Boram" has green and parted leaf, yellowish flower, and black seed coat. The ripening period of "Boram" is similar to "Sunmang", but 3 days faster than "Tammiyuchae". The average seed yield of the regional adaptation trials was 424 kg/10a, which was 11 and 48% higher than "Sunmang" and "Tammiyuchae" variety. Total oil content of "Boram" was 44.2%. In fatty acid composition, "Boram" has no erucic acid. However, oleic acid content is 68.5%, which is 3.7 and 5.4% higher than "Tammiyuchae" and "Sunmang". And total glucosinolate content was 2.27mg/g. Therefore this variety is recommended as a leading variety at southwestern areas including Jeonnam, Jeonbuk and Kyeongnam provinces and Jeju island of Korea.

• Korean Journal of Breeding Science
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• v.41 no.3
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• pp.194-204
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• 2009

Cytoplasmic male sterility (CMS) and fertility restoration have been utilized as valuable tools for $F_1$-hybrid seed production in many crops despite laborious breeding processes. Molecular markers for the selection of CMS-related genes help reduce the expenses and breeding times. A previously reported genomic region containing the Ppr-B gene, which is responsible for restoration of fertility and corresponds to the Rfo locus, was used to develop gene-based or so-called "functional" markers for allelic selection of the restorer-of-fertility gene (Rfo) in $F_1$-hybrid breeding of radish (Raphanus sativus L.) Polymorphic sequences among Rfo alleles of diverse breeding lines of radish were examined by sequencing the Ppr-B alleles. However, presence of Ppr-B homolog, designated as Ppr-D, interferes on specific PCR amplification of Ppr-B in certain breeding lines. The organization of Ppr-D, resolved by genome walking, revealed extended homology with Ppr-B even in the promoter region. Interestingly, PCR amplification of Ppr-D was repeatedly unsuccessful in certain breeding lines implying the lack of Ppr-D in these radishes. Ppr-B could only be successfully amplified for analysis through designing primers based on the sequences unique to Ppr-B that exclude interference from Ppr-D gene. Four variants of Rfo alleles were identified from 20 breeding lines. A combination of three molecular markers was developed in order to genotype the Rfo locus based on polymorphisms among four different variants. These markers will be useful in facilitating $F_1$-hybrid cultivar development in radish.

• Horticultural Science & Technology
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• v.33 no.3
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• pp.448-452
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• 2015

'Seeberry' is a short-day $F_1$ hybrid strawberry for seed propagation, that was released by the Protected Horticulture Experiment Station (RDA, Republic of Korea) in 2013. Inbred lines derived from domestic germplasms and selected varieties were used to develop this cultivar. 'Seeberry' originated from a cross of two inbred lines: a male parent 'Wongyo 3115' with high firmness and a female parent 'Wongyo 3116' with excellent fruit shape and high yield. 'Seeberry' cultivar should be grown with a crown diameter of over 10 mm to reveal its unique characteristics. 'Seeberry' has an upright plant shape with elliptic leaves, 12-15 flowers per cluster, vigorless growth habit, and average flower bud differentiation compared to vegetatively propagated cultivars. Fruits of 'Seeberry' are conical, having light skin color and approximately 15-16 g in average weight. Yield of this cultivar is about 92% lower than that of 'Akihime', but 'Seeberry' has excellent taste, sugar content of $9.7^{\circ}Bx$, acidity of 0.6%, and good texture. With regard to disease and pest resistance, 'Seeberry' is sensitive to powdery mildew, aphids, and the two-spotted spider mite, and is resistant to anthracnose.

• Korean Journal of Breeding Science
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• v.42 no.3
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• pp.298-301
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• 2010

A new intermediate parent 'Wonye 30002' (Allium cepa L.) was developed by Bioenergy Crop Research Center, NICS in 2009. As a male sterile line, 'Wonye 30002' can be used to hybrid seed production by crossing with pollen parent. The first cross was conducted between male sterile plants of 402AC203 and M1 in 2002. The male sterile line 'Wonye 30002' has circular bulb and bulb weight is 283 g. As early maturing type, lodging date is May 6. Plant height and pseudostem diameter are 43 cm and 15.5 mm, respectively. In seed harvesting characteristics, number of flower stalks and the length are 43 cm and 110 cm, respectively. The flowering date of 'Wonye 30002' is around May 24 and is completely male sterile. 'Wonye 30002' is a promising male sterile line for hybrid bulb onion seed production.

• Journal of Life Science
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• v.17 no.4 s.84
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• pp.493-497
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• 2007

The study were performed genetic analysis for seed characters of 6 parents of rice cultivar and 15 $F_1$ hybrids. In terms of heritability, The highest value was observed in the character of No. of grains/spike. Second values was showed 1,000 grains weight and head rice ratio was high value than grain length and width. In an analysis of correlation relation of parent, significant positive coefficients were observed in head rice ratio and No. of grains/spike, grain length and 1,000 grains weight, but the negative coefficients were significantly revealed between grain length and No. of grains/spike. Phenotype coefficient was lowly observed in the relation of grain length and 1,000 grains weight, grain width and 1,000 grain weight that highly positive genetic coefficient was showed in an analysis of correlation relation of $F_1$. In terms of heterosis, highest value was showed 1,000 grains weight and second value was No. of grains per spike and 1,000 grains weight and second value was No. of grains per spike in terms of heterobeltiosis. In an analysis heterosis of among crossing combination, high ratio was observed 1st combination at seed length, 12th combination at seed width, 3rd combination at head rice ratio, 5th combination at 1,000 grains weight and 11th combination at No. of grains per spike. In an analysis heterobeltiosis of among crossing combination, high ratio was observed 1st combination at seed length, 12th combination at seed width, 3rd combination at head rice ratio, th combination at 1,000 grains weight and 11th combination at No. of grains per spike.

• Horticultural Science & Technology
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• v.33 no.1
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• pp.143-148
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• 2015

A new Dianthus cultivar 'URI 2010-6' was selected among F1 progenies from a cross between interspecific hybrid 'Evergreen Emerald' as the seed parent and D. chinensis ' Ruby' as the pollen parent. In 2007, the cross w as made, and the seedlings of $F_1$ progenies were obtained and investigated. After three rounds of characteristic evaluation from 2008 to 2010, a clone was selected and registered as 'URI 2010-6' at the Korea Seed and Variety Service (plant variety protection number: 4431) in 2011. The flower of 'URI 2010-6' is caryophyllaceous, single type, and mainly bright red (Red, N79C) with secondary white (White, N155C) on the edge or as spots on the petal. The flower has diameter of 2.7 cm and length of 0.8 cm. This cultivar blooms continuously about 150 d on average and may be used as evergreen plant during winter months.