• 대한화장품학회지
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• 제49권2호
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• pp.183-192
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• 2023

본 연구에서는 황금 70% 에탄올 및 열수 추출물의 항산화, 항염, 미백, 보습, 세포 재생 및 자외선 및 블루라이트에 대한 세포 보호 효능을 확인하였다. 항산화 실험 결과, 70% 에탄올 및 열수 추출물 모두 DPPH 라디칼 소거 활성을 나타내었으며, 항염 실험 결과, 70% 에탄올 및 열수 추출물 모두 NO, 전염증성 cytokine(IL-6) 및 PGE₂의 생성을 억제하는 것을 확인하였다. 미백 실험 결과, 70% 에탄올 추출물과 열수 추출물 모두 멜라닌 생성을 저해하였으며, 보습 실험 결과, 70% 에탄올 및 열수 추출물 모두 보습 인자인 HA의 생성량이 증가시키는 것을 확인하였다. UVB 및 블루라이트에 대한 세포 보호 효과를 분석한 결과, 70% 에탄올 추출물과 열수 추출물 모두 UVB에 대한 세포 보호 효능을 나타내었으며, 70% 에탄올 추출물은 블루라이트에 대한 세포 보호 효능 또한 나타내었다. 또한, 세포 이동 및 증식 효능을 분석한 결과, 70% 에탄올 추출물에서 세포 성장이 촉진되는 것을 확인하였다. 이러한 결과를 바탕으로 황금 70% 에탄올 추출물, 열수 추출물은 항산화, 항염, 미백, 피부재생, 자외선 및 블루라이트에 대한 세포 보호 효과가 있는 화장품 관련 천연소재로써의 활용가능성이 있는 것으로 사료된다.

• 한국환경과학회지
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• 제17권12호
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• pp.1373-1380
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• 2008

Anthracene appended new host compounds have been synthesized by imine reaction. Fluorescent open chain host compounds Trisanthryl-tris(2-aminoethyl)imine 1 was synthesized from the reaction of tris(2-aminoethyl)amine and anthracene-9-carboxaldehyde in EtOH. Tris-10-chloroanthryl-tris(2-aminoethyl)imine 2 was synthesized from tris(2-aminoethyl)amine and 10-chloro-9-anthraldehyde in EtOH. The structures of all reaction product were identified by $^1H$ NMR, $^{13}C$ NMR, GC/MS, FAB Mass, IR spectrum and DSC. Cation complexation behavior was investigated by fluorescence spectroscopy measurements. The capability of transition metals cation recognition between fluorescent open chain host compound 1, 2 were investigated with $Co^{2+},\;Ni^{2+}\;and\;Cu^{2+}$. The fluorescence intensity was increased by host compounds corresponding guest cations. The relative order of fluorescence intensity changes were $Co^{2+}>Cu^{2+}>Ni^{2+}$. Compound 2 is very sensitive fluorescent sensor of $Co^{2+}$ ion.

• 천문학회보
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• 제38권1호
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• pp.62.1-62.1
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• 2013

The Cassini/Visual Infrared Mapping Spectrometer (VIMS) observed the sun through the atmosphere of Titan, and provided vertically-resolved 63 spectra from 49 km to 987 km for the 1 - 5 micron range (Bellucci, 2008). Bellucci et al. (2009) analyzed selected spectral ranges where the band absorptions of $CH_4$ and CO are strong by constructing synthetic spectra including $CH_4$ and CO lines, but without including haze absorptions in their synthetic spectra. Kim et al. (2011) and Sim et al. (2013) were able to extract detailed spectral features of fundamental (Dv = 1) and overtone (Dv = 2) bands of the haze from the VIMS spectra by excluding the adjacent influences of strong $CH_4$ absorptions using a radiative transfer program, which includes effects of absorption and emission of lines of these molecules, and absorption and scattering of haze particles. In this presentation, we extend our detailed analyses to other remaining wavelengths in order to provide the spectral characteristics of the Titanian haze for the entire 1 - 5 micron range and to identify any additional haze spectral features and an unidentified feature near 4.3 microns reported by Bellucci et al. (2009).

• Clinical and Experimental Reproductive Medicine
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• 제26권3호
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• pp.419-432
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• 1999

Objective: To establish the evaluation system of the quality of oocytes on the basis of the incidence of cumulus cells apoptosis, to investigate the relationships beween the incidence of cumulus cells and the outcomes of IVF-ET. Method: Thirth-four cycles undergoing controlled ovarian hyperstimulation for IVF-ET with tubal infertility (23 cycles) or unexplained infertility (11 cycles) were included in this study. Cumulus cell masses surrounding mature oocyte and co-culture of embryos with autologous cumulus cells during IVF-ET process. The incidence of apoptosis in cumulus cells was assessed by apoptosis detection kit fluorescein. The effect of co-culture using cumulus cells and the incidence of cumulus cells apoptosis. Results: The results were as follows: 1. The incidence of apoptosis in cumulus cells markedly increased in patients aged 40 or over, while the fertilization rate was greatly decreased in those age group. 2. Apoptosis in cumulus cells was found in both the fertilized oocytes and unfertilized oocytes, but the incidence of apoptosis was higher in unfertilized oocytes. 3. There is no clear correlation between apoptosis in cumulus cells and the number of oocytes retrieved. However, the incidence of apoptosis was increased when the number of oocytes retrieved was 5 and fewer in comparison with $6{\sim}10$. 4. Embryo grade was significantly affected by the incidence of apoptosis in cumulus cells. 5. Pregnancy rate of IVF-ET per cycle was 29.4%, and the pregnant group had the higher fertilization rate and a significantly lower incidence of apoptosis in cumulus cells compared with the nonpregnant group. 6. When cumulus cells were used as helper cells in the co-culture of the embryo, in vitro activity of cumulus cells based on morphological change and proliferation did not influence the quality of embryo, but was closely associated with the implantation rate and pregnancy rate, which was enhanced when morphological changes and proliferation of cumulus cells was more active. 7. This difference in the outcome of IVF-ET according to in vitro activity of cumulus cells used for co-cultue was not associated with the incidence of apoptosis in cumulus cells; but rather had likely relations with the different secretion pattern of protein, which may be an embryo trophic factor by cumulus cells. Conclusion: These results suggest that the incidence of apoptosis in cumulus cells can be used in predicting oocyte qualities and the outcomes of IVF-ET. And the effect of co-culture largely depends on the in vitro activity of cumulus cells as well.

• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 2021년도 춘계학술대회
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• pp.49-49
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• 2021

The roots of reed (Phragmites australis) were used in traditional medicine to treat respiratory problems, including symptoms such as high fever and cough. In this study, we identified the active ingredient from 70% EtOH reed root extract, and evaluated the whitening, wrinkle improvement and moisturizing effects. The content of p-coumaric acid, the active ingredient of the roots of P. australis, was slightly lower in 70% EtOH extract than in 100% EtOH extract. However, 70% EtOH reed root extract showed similar or higher effect in reducing power, DPPH, hydrogen peroxide scavenging, and nitric oxide scavenging activity compared to 100% EtOH extract. Moreover, 70% EtOH reed root extract markedly inhibited melanogenesis in B16F10 cells treated with α-melanocyte-stimulating hormone. 70% EtOH reed root extract significantly inhibited the mRNA expression of matrix metalloproteinase-1 (MMP-1) and reduced elastase activity in HDF human dermal fibroblasts. In addition, 70% EtOH reed root extract ameliorated hyaluronic acid synthase-2 (HAS-2) expression induced by ultraviolet B (UVB) stimulation in HaCaT keratinocytes. The results of this study suggest that 70% EtOH reed root extract has potential as a functional cosmetic material related to whitening, wrinkle improvement, and moisturizing.

• Biotechnology and Bioprocess Engineering:BBE
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• 제6권4호
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• pp.301-305
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• 2001

The co-expression of the arg U gene in a double-vector expression system of recombi-nant Escherichia coli BL22(DE3)[pET-IEN2a+pAC-argU] significantly enhanced the production level of reconminant human interferon -$\alpha$2a(rhIFN-$\alpha$2a) in high cell density cultures, compared to a recombinant E. coli culture containing only the single expression vector, pET-IEN2a. The dry cell mass concentration increased to almost 100 g/L, and more than 4 g/L of rhIFN-$\alpha$2a was accumu-lated in the culture broth. Evidently, the synthesis of rhIFN-$\alpha$2a was strongly dependent on the pre-induction growtih rate and more efficient at a higher specific growth rate. The additional sup-ply of tRN $A^{Arg(AGG/AGA)}$ enhanced the expression level of the rhIFN-$\alpha$2a gene in the early stage of the post-induction phase, yet thereafter the specific production rate of rhIFN-$\alpha$2a rapidly de-creased due to severe segregational instability of plasmid vector pET-IEN2a. It would appear that the plasmid instability with only occurred to pET-IEN2a in the double vector system, was re-lated to the effect of translational stress due to the over expression of rhIFN-$\alpha$2a.

• Journal of Acupuncture Research
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• 제32권4호
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• pp.195-201
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• 2015

Objectives : The purpose of this study is to report on the improvement of respiratory function of a fascioscapulohumeral muscular dystrophy patient after receiving Korean medical treatment. Methods : This study was carried out on a 60 year-old male patient who suffered from fascioscapulohumeral muscular dystrophy. While we carried out Korean medical treatment, we observed $EtCO_2$ (end-tidal carbon dioxide), Vte (exhaled tidal volume) and PIP (peak inspiratory pressure). Results : $EtCO_2$ count decreased from 34 mmHg to 24 mmHg during the treatment period, and PIP count became stable. Vte count also did not worsen in this study. Conclusions : Based on this study, we concluded that Korean medical treatment could be effective in treating fascioscapulohumeral muscular dystrophy patients with respiratory failure.

• Bulletin of the Korean Chemical Society
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• 제17권9호
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• pp.802-807
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• 1996

Second-order rate constants have been measured spectrophotometrically for the reactions of aryl benzoates (X-C6H4CO2C6H4-Y) with EtO-, Z-C6H4O- and Z-C6H4C(Me)=NO- in absolute ethanol at 25.0 ℃. All the reactions have been performed in the presence of excess 18-crown-6 ether in order to eliminate the catalytic effect shown by alkali metal ion. A good Hammett correlation has been obtained with a large ρ- value (-1.96) when σ- (Z) constant was used for the reaction of p-nitrophenyl benzoate (PNPB) with Z-C6H4O-. Surprisingly, the one for the reaction of PNPB with Z-C6H4C(Me)=NO- gives a small but definitely positive ρ- value (＋0.09). However, for reactions of C6H5CO2C6H4-Y with EtO-, correlation of log k with σ- (Y) constant gives very poor Hammett correlation. A significantly improved linearity has been obtained when σ0 (Y) constant was used, indicating that the leaving group departure is little advanced at the TS of the RDS. For reactions of X-C6H4CO2C6H4-4-NO2 with EtO-, C6H5O- and C6H5C(Me)=NO-, correlations of log k with σ (X) constants for all the three nucleophile systems give good linearity with large positive ρ values, e.g. 2.95, 2.81 and 3.06 for EtO-, C6H5O- and C6H5C(Me)=NO-, respectively. The large ρ values clearly suggest that the present reaction proceeds via a stepwise mechanism in which the formation of the addition intermediate is the RDS.

• 콘크리트학회논문집
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• 제28권4호
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• pp.427-434
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• 2016

이 연구의 목적은 인공경량골재 콘크리트의 생산에서 $CO_2$ 배출량을 저감시킬 수 있는 천연모래 치환 기반의 배합설계 절차를 제시하는 것이다. 379개의 경량골재 콘크리트 배합 데이터를 사용하여 $CO_2$ 배출량과 콘크리트의 압축강도에 대한 천연모래의 치환율의 영향을 평가하였다. Yang et al.이 제시한 배합설계절차 및 데이터베이스를 이용한 비선형 회귀 분석에 기반하여 목표 성능(압축강도, 초기 슬럼프, 공기량 및 $CO_2$ 저감률)을 만족하기 위한 천연모래의 치환율 및 콘크리트 배합설계를 결정할 수 있는 간단한 모델식을 제시하였다. 뿐만 아니라, 제안된 모델식은 주어진 경량골재 콘크리트 배합표에서 $CO_2$ 배출량을 직접 계산하는데 효율적으로 이용될 수 있다.

• 천문학논총
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• 제30권2호
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• pp.103-105
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• 2015

The 22 m diameter Mopra telescope in Australia is being used to undertake an improved survey of the CO J = 1-0 line at 3mm along the 4th quadrant of the Galaxy, achieving an order of magnitude better spatial and spectral resolution (i.e. 0.6 and 0.1 km/s) than the Dame et al. (2001) survey that is publically available for the Southern Galactic plane. Furthermore, the Mopra CO survey includes the four principal isotopologues of the CO molecule (i.e. $^{12}CO$, $^{13}CO$, $C^{18}O$ and $C^{17}O$). The survey makes use of an 8 GHz-wide spectrometer and a fast mode of on-the-fly mapping developed for the Mopra telescope, where the cycle time has been reduced to just 1/4 of a second. 38 square degrees of the Galaxy, from $l=306-344^{\circ}$, $b=0{\pm}5^{\circ}$ have currently been surveyed, together with additional 9 sq. deg. regions around the Carina complex and the Central Molecular Zone. We present new results from the survey (see also Burton et al., 2013, 2014). The Mopra CO data are being made publically available as they are published; for the latest release see the project website at www.phys.unsw.edu.au/mopraco.