• Korean Journal of Microbiology
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• v.23 no.1
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• pp.9-12
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• 1985

As a part of the host cell development for a amplified recombinant trp operon, $aroP^-$ mutation was introduced in a E. coli host strain. $aroP^-$ mutation was induced by transposon Tn10 and transduced into the E. coli host cell by bacteriophage P1Kc. The effect of $aroP^-$ mutation on the excretion of tryptophan in E. coli $trpR^{-ts}/ColE_1 -trp^+$ cells was investigated. Mutant lacking the general aromatic transport system was resistant to ${\beta}-2-thienylalanine\;(2{\times}10^{-4}\;M)$, p-fluorophenylalanine $(2{\times}10^{-4}M)$, or 5-methyltryptophan $(2{\times}10^{-4}\;M.)[^3H]-tryptophan$ uptake of the $aroP^-$ mutant strain was reduced considerably as compared with $aroP^+$ counterpart. The rate of $[^3H]-tryptophan$ uptake of the $aroP^-$ mutant strain treated with $NaN_3(3{\times}10^{-2}\;M)$ was much less affected than that of $aroP^+$ counterpart. The $aroP^-$ transductants increased the tryptophan excretion from E. coli $trpR^{-ts}/ColE_1 -trp^+$ four times more than $aroP^+$ counterpart.

• Journal of Engineering Education Research
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• v.15 no.4
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• pp.109-117
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• 2012

The purpose of this study is to give the preliminary data and suggestion for introducing and spreading e-learning engineering education through analyzing learning behaviors, learning motivations, and satisfaction of e-learning engineering students. Especially, this comparative study analyzes each research domain according to majors and grades, thereby suggesting more specific and practical results. 2,745 students registered in 38 subjects of e-learning in 2 Universities were analyzed for this study. The study result shows that engineering students are attending around 2 e-learning subjects with a duration of about 30 minutes once a week. The main of learning motivation for e-learning was not easy test level and feasibility of acquiring credit but advantages of e-learning such as freedom of time and space, learning by repetition. The satisfaction scores of e-learning were lower compared to the aspects of system and contents Based on these results, first, an active spread of e-learning to engineering education is necessary because the demand from the engineering students is high enough and they have desirable learning behavior and learning motivation for it. Second, the characteristics of grades need to be taken into consideration on operation of e-learning. Third, a successful e-learning process needs more meticulous and active operation.

• THE INTERNATIONAL COMMERCE & LAW REVIEW
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• v.47
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• pp.239-260
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• 2010

The purpose of this study would like to investigate the critical success factors of the e-procurement which means on-line purchase of goods and services for organizations. This action represents one of the largest expense items in a firm's cost structure. In order to draw CSFs of e-procurement system, we have surveyed questionnaire to the related firms. The basic variables used in this survey are taken from many precedent researches in relation to the e-procurement. On the other hand, the research data is collected from companies which had used the MRO e-marketplace. Through factor analysis of those data, four-factor resulted as follows: (1) factor one suggests the rationalization of the firm's management of its suppliers; (2) factor two calls for redesigning affected business processes and influencing end-user/employee procurement-related behaviors; (3) factor three refers to carefully orchestrating an e-procurement technology planning process with one's suppliers and using intelligence in designing the software and mining the data it produces; and (4) factor four relates to selecting an e-procurement solution and/or simultaneously participating in a number of electronic environments supporting e-procurement. In conclusion, these four factors are regarded as critical success factors for the application of e-procurement system and then suppliers should take care the effective planning of e-procurement structure.

• Journal of Life Science
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• v.10 no.5
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• pp.529-532
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• 2000

Prostaglandin $E_2$(PG$E_2$) is an abundant eicosanoid in bone that has been implicated in a number of pathological states associated with bone loss, and is also known to stimulate matric metalloproteinase-1 systhesis and secretion in rat and human osteoblast cells, although the intracellular reaction remain unclear. Interleukin-1$\beta$ (IL-1$\beta$) is a cytokine that plays a critical role in bone remodelling and appears to act as a downstream effector of most bone-resorbing agents. However, it is still interesting to examine whether PG$E_2$ regulates IL-1$\beta$ expression by mouse osteoblasts or not. Here we demonstrate that PG$E_2$is a potent inducer of IL-1$\beta$ production by fetal osteoblasts.

• Journal of Korean Society on Water Environment
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• v.22 no.6
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• pp.1144-1150
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• 2006

Recently, as water pollution accidents in rivers have increased, there is an increased interest in water quality forecast with accurate simulation. QUAL2E model, widely used for water quality analysis, uses the same hydraulic characteristics, such as depth and velocity, in a reach. The flow of the river is changed by various hydraulic constructions or by topography in a real river channel. In this study, a hydraulic connection module is developed to consider flow variations of river channels in QUAL2E model. The module uses the simulations results of non-uniform flow of a 1-D hydraulic model such as DWOPER or HEC-RAS. The improved QUAL2E model with this module was applied to a downstream section of Paldang Dam on the Han River. The results show the variation of water quality very well in a reach where flowing vary abruptly, like the Jamsil submerged weir.

• Asian Pacific Journal of Cancer Prevention
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• v.17 no.7
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• pp.3065-3069
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• 2016

Single nucleotide polymorphism (SNP) detection has been used extensively for genetic association studies of diseases including cancer. For mass, yet accurate and more economic SNP detection we have optimized tetra primer amplification refractory mutation system polymerase chain reaction (ARMS PCR) to detect three SNPs in the cytochrome P450 2E1 (CYP2E1) gene locus; i.e. rs3813865, rs2070672 and rs3813867. The optimization system strategies used were (1) designing inner and outer primers; (2) determining of their optimum primer concentration ratios; and (3) determining of the optimum PCR annealing temperature. The tetra primer ARMS PCR result could be directly observed using agarose gel electrophoresis. The method succesfully determined three SNPs in CYP2E1 locus, the results being consistent with validation using DNA sequencing and restriction fragment length polymorphisms (RFLP).

• Transactions of the Korean Society of Mechanical Engineers B
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• v.29 no.1 s.232
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• pp.35-45
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• 2005

TR-E2 configuration designed by Bell Textron was tested in KARI 1-m wind tunnel. To explore aerodynamic characteristics for the given configuration, a $12\%$ scaled model was fabricated and tested. Wind tunnel test for TR-E2 had been performed by changing the incidence angles of wing and deflection angles of control surfaces. Test result showed that the lower wing incidence angle has more favorable lift to drag ratio compared with original design. Longitudinal and directional characteristics of TR-E2 were found to be stable for the pitch and yaw motions. However, the lateral stability of TR-E2 is not stable for certain control surface deflection.

• Proceedings of the Korea Water Resources Association Conference
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• 2012.05a
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• pp.780-780
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• 2012

하천에서의 1차원 수질해석에 널리 사용되는 QUAL2E 모형은 전체 대상구간을 요소(element)로 구성하고 다시 수리학적으로 유사한 흐름특성을 가진 구간(reach)으로 구분하고 있다. 각 구간에서는 동일한 유속과 수심이 형성되는 것으로 가정하고 있기 때문에 자연하도의 다양한 흐름변화, 특히 자연하천의 보나 낙차공으로 인한 국부적인 흐름상태를 모형에 반영하기 어려우며, 이로 인한 실제 흐름특성과의 차이는 수질모의 해석결과에도 다양한 영향을 미치게 된다. QUAL2E 모형에서는 이와 같은 보나 낙차공의 수리학적 특성을 반영하기 위해 댐 계수옵션이 있으며, 이를 통해 흐름으로 인한 폭기 현상을 재현하도록 되어 있다. 본 연구에서는 모형 내 재폭기 계수에 대한 민감도 검토를 수행하였으며, 한강의 팔당댐부터 한강대교까지를 대상구간으로 잠실수중보로 인해 발생하는 재폭기 현상에 관해 연구하였다. 본 연구는 보나 낙차공이 있는 하천에 대해 QUAL2E 모형을 적용할 경우 용존산소(DO)를 비롯한 여러 가지 수질변수의 정확도 높은 예측에 기여할 것으로 기대된다.

• Bulletin of the Korean Chemical Society
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• v.7 no.3
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• pp.186-190
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• 1986

Effects of substituents in the nucleophile(X), the substrate(Y) and the leaving group(Z) on the structure of $S_N2$ transition states have been analyzed by considering effects of four components, electrostatic($E_{es}$), exchange repulsion ($E_{ex}$), polarization($E)_{pl}$) and charge transfer($E_{ct}$) terms, of interaction between the reactants on the degree of bond making and bond breaking. Prediction of net effects of all substituents(X, Y and Z) on the degree of bond making were found to be clearcut whereas the effect of an electron withdrawing group on the substrate (Y = EWG) on the degree of bond breaking was complex; the substituent(Y = EWG) is normally carbon-leaving group($C^{\ast}$-L) bond tightening($E_{pl}$ dominance) but becomes $C^{\ast}$-L bond loosening when the bond is strongly antibonding ($E_{ct}$ dominance). Our model calculations on the reaction of $CH_2XNH_2$ with $YCH_2COOCH_2Z$ using energy decomposition scheme have confirmed that predictions based on our analysis are correct.

• Rapid Communication in Photoscience
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• v.1 no.2
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• pp.42-42
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• 2012