• Journal of Plant Biology
• /
• 제37권3호
• /
• pp.349-355
• /
• 1994

Viral RNA was extracted from a purified orchid strain of tobacco mosaic virus (TMV-O) from Cymbidium "Grace Kelly". Polyadenylated viral RNAs were primed with Not I-oligo (dT) primer-adapter. First-strand cDNAs were reversely transcribed by Moloney murine leukaemia virus reverse transcriptase (RNAse H-), and then second-strand cDNAs were synthesized by RNase H and DNA polymerase I. The resulting double-stranded cDNAs were ligated into pSPORT1 vector and transformed into competent E. coli strain JM109 cells. The size of cDNAs within the recombinant plasmids was ranging from 0.9 to 3.9 kb. Among the selected clones, pTMO-0205 and -0210 covered the 3' half and the 5' half of the viral genomic RNA, respectively, which were covering more than 99% of the viral genemo size based on sequencing analysis. Two cDNA fragments which were 3.1 kb BamHI and NotI fragement released from pTMO-0.205 and 3.3 kb SalI and BamHI fragment released from pTMO-0210 were ligated with T4 DNA ligase. The clone was almost entire length, lacking only 31 nucleotides from the 5' terminus based on the sequencing result. This method was shown to be efficiently applicable to other plant viral gnomic RNA for the construction of cDNA.n of cDNA.

• E2M - 전기 전자와 첨단 소재
• /
• 제3권3호
• /
• pp.224-232
• /
• 1990

SnO$_{2}$를 모체로 한 가스 감지소자의 가스 감지특성을 여러 감지특성을 여러 가스에 대하여 조사하였다. 2ton/$cm^{2}$의 압력에서 성형한 pellet형 소자를 온도 500-800.deg.C 범위에서 1-5시간 동안 소성시켰다. 이때 소자의 Pd함량은 0.0wt%까지 변화되었다. 측정온도에 대항 감도의 의존성 및 가스에 대한 응압시간등도 조사하였다. 여러 감도변수에 따른 소성온도의 영향을 SnO$_{2}$ 분말의 소결성으로 설명하였다.

• Transactions on Electrical and Electronic Materials
• /
• 제13권6호
• /
• pp.297-300
• /
• 2012

Lead-free (1-x)$(Na_{0.5}K_{0.5})NbO_3-xLiNbO_3$, i.e., NKN-LNx (x=0.0, 0.1, 0.2, 0.3, 0.4 mol) was prepared using the conventional solid state reaction method. The effects of LN mixing on the ferroelectric properties of NKN-LNx ceramics were studied using a dielectric constant and P-E (Polarization-electric field) measurements. Ferroelectricity was observed in the composition for x approximately varying between 0.0 and 0.4. Minimum remanent polarization $2P_r=5C/cm^2$ was achieved in the composition for x = 0.2. The ferroelectric phase transition temperature $T_C$ increased with increasing LN content. The ferroelectric phase transition of NKN-LNx ($x{\geq}0.1$) is a second-order phase transition, and that of NKN-LNx ($x{\leq}0.2$) is a first-order phase transition. These results indicate that the ferroelectric phase transition temperature of NKN-LNx change from that of second-order to weak first-order phase transition according to the LN content.

• 대한약학회:학술대회논문집
• /
• 대한약학회 2001년도 Proceedings of the Pharmaceutical Society of Korea
• /
• pp.213.3-214
• /
• 2001

• 한국재료학회:학술대회논문집
• /
• 한국재료학회 1998년도 IUMRS-ICEM ABSTRACT BOOK
• /
• pp.118.2-118
• /
• 1998

• 반도체디스플레이기술학회지
• /
• 제10권1호
• /
• pp.95-99
• /
• 2011

$(1-x)BaTiO_3+(x)Cd_5(PO_4)_3Cl$ ceramics were prepared by the conventional ceramic technique, i.e., solid state reaction at high temperature. The concentration of $Cd_5(PO_4)_3C$ was varied from 0.01 to 0.15 mole fraction. In order to study the phase transitions of our ceramics, the Raman scattering spectra were measured as functions of concentration x and temperature. It was found that the soluble limit of $Cd_5(PO_4)_3Cl$ in $BaTiO_3$ was the x=0.05 composition and $BaTiO_3$ phase disappeared above x=0.10. A new phase identified as $Ba_4Ti_3P_2O_{15}$ was detected in all samples of our compositions. The Curie temperature shifts up to $130^{\circ}C$ as the concentration x increases from zero to 0.05 and shift down to $95^{\circ}C$ as further increases to 0.08. For the increase of the Curie temperature, it is suggested that it can result from the inhibition of displacement of $Ti^{4+}$ in the distorted octahedron due to well dispersed $Ba_4Ti_3P_2O_{15}$ and $Cd_5(PO_4)_3Cl$ phase.

• 한국작물학회:학술대회논문집
• /
• 한국작물학회 1988년도 춘계 학술대회지
• /
• pp.104-105
• /
• 1988

• 한국전기전자재료학회논문지
• /
• 제15권9호
• /
• pp.788-793
• /
• 2002

The glass-electroceramics were composed of glass composition(CaO, $SiO_2$, $B_2$ $O_3$) and electroceramic composition(BaO, N $d_2$ $O_3$, B $i_2$ $O_3$ and Ti $O_2$) Their dielectric properties have been investigated as a function of sintering temperature and glass contents. In the ceramics composed of 0.16BaO-0.15(N $d_{0.87}$,B $i_{0.13}$)$_2$ $O_3$-0.69Ti $O_2$with glass ［EG-2782］ 3wt% addition and sintered at 108$0^{\circ}C$ for 2h, we could obtain microwave properties of dielectric constant $\varepsilon$$_{r}$ = 80.1, quality factor Q $\times$f = 810(at 3.5 GHz) and temperature coefficient of resonant frequency $\tau$$_{f}$ = -1.3 ［ppm/$^{\circ}C$］. These experimental results show that dielectric constant and temperature coefficient of resonant frequency could be estimated by empirical equations involving the rule of mixture.e.

• 음성과학
• /
• 제8권4호
• /
• pp.171-184
• /
• 2001

American English vowels and Korean vowels were compared by the plotformant method. For American English vowels, six General American English speakers pronounced English words in the b_t environment. For Korean vowels eight Kyongsang dialect speakers and eight Seoul dialect speakers pronounced Korean words in the environments of k_t, p_t and t_t. The formant plots were obtained by plotting F1/F2 tokens of 13 American English vowels on the F1xF2 plane. In spite of personal variations the 13 vowel spaces of all six American English speakers maintained their relative positions with some overlaps. Clear distinctions were made between i-I, e-$\varepsilon$, u-$\sigma$, and o-c. The domain of c and $\alpha$ overlapped for three American English speakers, but it did not for three other speakers. The 8 Korean vowel spaces of Kyongsang dialect speakers and Seoul dialect speakers were very similar and maintained their relative positions. No distinction was made between e and $\varepsilon$. In contrast with American English e which is a neutral vowel, Korean e was a back vowel. The comparison of 13 American English vowel positions and 8 Korean Vowel positions is expected to shed some light on the errors of English vowel pronunciation of Korean learners.

• Bulletin of the Korean Chemical Society
• /
• 제35권6호
• /
• pp.1706-1712
• /
• 2014

The OH($X^2{\Pi}$, ${\upsilon}^{\prime\prime}=0,1$) internal state distribution following the reaction of electronically excited oxygen atom ($O(^1D_2)$) with cyclo-$C_3H_6$ has been measured using laser-induced fluorescence, and compared with that following the reaction of $O(^1D_2)$ with $C_3H_8$. The overall characteristics of the OH internal energy distributions for both reactions were qualitatively similar. The population propensity of the ${\Pi}(A^{\prime})$ ${\Lambda}$-doublet sub-level suggested that both reactions proceeded via an insertion/elimination mechanism. Bimodal rotational population distributions supported the existence of two parallel mechanisms for OH production, i.e., statistical insertion and nonstatistical insertion. However, detailed analysis revealed that, despite the higher exoergicity of the reaction, the rotational distribution of the OH following the reaction of $O(^1D_2)$ with $C_3H_8$ was significantly cooler than that with cyclo-$C_3H_6$, especially in the vibrational ground state. This observation was interpreted as the effect of the flexibility of the insertion complex and faster intramolecular vibrational relaxation (IVR).