• Korean Journal of Food Science and Technology
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• v.21 no.5
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• pp.619-623
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• 1989

The cultural condition of Pseudomonas mendocina for polysaccharide production was examined. The optimal medium contains following composition per liter of distilled water: Sucrose 23.75g, $(NH_4)_2SO_4$ 1.57g, Yeast extract 0.5g, $KH_2PO_4\;2.9g,\;MgSO_4.\;7H_2O\;1.0g,\;CaCO_3$ 2.5g. The optimum temperature and pH were $30^{\circ}C$ and 6.5. At the condition. Ps mendocina produced 5.98g/l of polysaccharide. The culture viscosity after 3 days was 191mPa.s at $70sec^{-1}$. The product yield $(Y_{p/s})$ and specific productivity $(Q_p)$ were 25.18% and 32.83mg/g-cell/h.

• Journal of Bio-Environment Control
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• v.19 no.4
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• pp.298-304
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• 2010

Nutrient uptake by plants and drainage ratio in culture beds can affect ion balance and concentrations of nutrient solutions in electrical conductivity (EC)-based closed-loop soilless culture. This study was conducted to analyze ion concentration changes with time and drainage ratio under EC-based nutrient control in closed-loop soilless culture for sweet pepper plants (Capsicum annum L. 'Boogie'). At first experiment, ion concentrations of the nutrient solution were periodically analysed while collected drainage was being reused by mixing with fresh nutrient solution at a dilution rate of EC $2.2\;dSm^{-1}$. Changes in ion concentrations of $K^+$, $Ca^{2+}$, $Mg^{2+}$, $NO_3^-$, $SO_4^{2-}$, and $PO_4^{3-}$ were 1.13, 5.35, 0.92, 0.9, 1.10, $0.19\;meq{\cdot}L^{-1}$, respectively. Ion balance such as $K^+$ : $Ca^{2+}$ and $SO_4^{2-}$ : $NO_3^-$ were mainly affected during the recirculation of nutrient solution. At second experiment, ion concentrations and EC of drainage were compared before and after replenishment under different four drainage ratios of 7%, 16%, 39%, and 51%. Ion ratios of the recirculated nutrient solutions such as $K^+$ : $Ca^{2+}$ for cation and $SO_4^{2-}$ : $NO_3^-$ for anion were investigated. ECs of drainage decreased with increase of drainage ratio and each ion concentration showed the same trends as EC did. Ion balances in drainage with drainage ratio were a little different from each other, but each ratio could be corrected by replenishment process. The ion balance at 7% drainage ratio was closest to initial ratio and followed by 16%, 51%, and 39% in the order. Ion balance such as $K^+$ : $Ca^{2+}$ and $NO_3^-$ : $PO_4^{3-}$ were mainly affected the correction process.

• Journal of the Korean Crystal Growth and Crystal Technology
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• v.30 no.4
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• pp.143-149
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• 2020

Calcium phosphates recognized as important bio-materials have been successfully synthesized by simple precipitation using waste abalone shells, which are rich mineral sources of calcium. Calcium hydroxide (Ca(OH)₂) originated from abalone shells was used as calcium source (precursor) for the preparation. Synthesis of calcium phosphates was performed by reacting calcium hydroxide with phosphoric acid (H₃PO₄) in aqueous solution. The initial precursor Ca/P ratios were adjusted to 1.50, 1.59 and 1.67, and the effect of the composition and the heat treatment on the synthesized powders and sintered bodies was investigated. The phases of the sintered ceramics prepared at 1150℃ were hydroxyapatite (HAp), β-tricalcium phosphate (β-TCP), and biphasic phosphate (HAp with β-TCP)), which were determined by the initial precursor Ca/P ratios. The results demonstrate the possibility for the synthesis of high value-added calcium phosphates from economical starting materials with low cost and high availability.

• Bulletin of the Korean Chemical Society
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• v.28 no.4
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• pp.652-656
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• 2007

Hydroxyapatite [Ca10(PO4)6(OH)2, HAp] and titanium(Ti) metal are known to be excellent materials with high affinities for natural bone through the apatite. Tricalcium phosphate [Ca3(PO4)2, TCP] is a promising alternative material because it is similar to HAp in its physical properties and biocompatibility. To examine the influence of hydroxyapatite, tricalcium phosphate, pure titanium and pre-treated titanium on osteopontin expression in osteoblasts, RNA was extracted from proliferated and cultured osteoblast cells and OPN mRNA expression was observed by RT-PCR.

• Applied Biological Chemistry
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• v.34 no.3
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• pp.213-218
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• 1991

Addition of salts or their mixtures and microwave heating were studies for their effects on Kakdugi fermentation. The Kakdugi, a Korean fermented Chinese radish, were prepared by salting in 15% NaCl solution and fermented at $25^{\circ}C$. From the results, a first order reaction relationship was found between salt permeated into the radish and time during brining in $5{\sim}10%$ NaCl solution. Addition of 0.05M KCl into 15% NaCl brining solution or microwave heating of salted radish for 3 minutes showed a little decreasing effect on Kakdugi fermentation rate while beating for 1 or 2 minuted resulted a rather increase. When three different salt mixtures in the concentration range of $0.001{\sim}0.01\;M$ were added into half fermented Kakdugi(appr. pH 4.4), the fermentation was greatly controlled based on pH change. Among the salt mixtures, mixture III$(Na_2HPO_4,\;Na_2PO_4,\;NaNO_2,\;Ca\;EDTA,\;Sod.\;citrate)$ showed a most significant effect where the time required to reach pH 4.0 after addition was extended by more than 6 folds when it was compared to the control method.

• Korean journal of applied entomology
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• v.28 no.2
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• pp.69-75
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• 1989

The extracellular amylase production by Bacillus thuringiensis subsp. kurstaki HD-l in amylase production media and its characteristics were investigated. The amylase production was highest in the medium composed of 0.2% soluble starch, 1.0% Bacto-peptone, 0.3% beef extract, 0.3% yeast extract, 0.5% NaCl, 0.3% $K_2HPO_4$, 0.1% $KH_2PO_4$, 0.012% $CaCl_2$.$2H_2O$, 0.005% $MnSO_4$.$H_2O$, and 0.03% $MgSO_4$.$7H_2O$. The amylase activity was inhibited by 50mM EDT A. The enzyme was optimally active from pH 6.5 to 7.0 at $55^{\circ}C$, The specific activity of the enzyme in the ethanol precipitate was 2.01 units/mg, and the Km value was approxi-mately 0.8 mg/ml.

• The Korean Journal of Ceramics
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• v.4 no.2
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• pp.151-155
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• 1998

The total electrical conductivity, ionic conductivity, and nonstoichimetry of a new cathode material $Y_{1-x}Ca_xFeO_{3-\delta}$ (x=0.1) were measured as functions of temperature ($900\leqT/^{\circ}C\leq1100$) and oxygen partial pressure $(10^{-6}\leqPo_2/atm\leq0.21$). Isothermal variations of these properties with $Po_2$ support that the majority type of ionic defects are anti-Frenkel disorder which, however, has seldom been considered for perovskite-based oxides. The results are discussed in comparison with those reported on similar oxides.

• Journal of the Korean Association of Oral and Maxillofacial Surgeons
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• v.34 no.1
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• pp.36-48
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• 2008

To repair bone defects in the oral and maxillofacial field, bone grafts including autografts, allografts, and artificial bone are used in clinical dentistry despite several disadvantages. The purpose of this study was to evaluate new bone formation and healing in rat calvarial bone defects using hydroxyapatite (HA, $Ca_{10}[PO_4]_6[OH]_2,\;Bongros^{(R)}$, Bio@ Co., KOREA) and tricalcium phosphate (${\beta}-TCP,\;Ca_3[PO_4]_2$, Sigma-Aldrich Co., USA) mixed at various ratios. Additionally, this study evaluated the effects of type I collagen (Rat tail, BD Biosciences Co., Sweden) as a basement membrane organic matrix. A total of twenty, 8-week-old, male Sprague-Dawley rats, weighing 250-300g, were divided equally into a control group (n=2) and nine experimental groups (n=2, each). Bilateral, standardized transosseous circular calvarial defects, 5.0 mm in diameter, were created. In each experimental group, the defect was filled with HA and TCP at a ratio of 100:0, 80:20, 70:30, 60:40, 50:50, 40:60, 30:70, 20:80, and 0:100 with or without type I collagen. Rats were sacrificed 4 and 8 weeks post-operation for radiographic (standardized plain film, Kodak Co., USA), histomorphologic (H&E [Hematoxylin and Eosin], MT [Masson Trichrome]), immunohistochemical staining (for BMP-2, -4, VEGF, and vWF), and elementary analysis (Atomic absorption spectrophotometer, Perkin Elmer AAnalyst $100^{(R)}$). As the HA proportion increased, denser radiopacity was seen in most groups at 4 and 8 weeks. In general radiopacity in type I collagen groups was greater than the non-collagen groups, especially in the 100% HA group at 8 weeks. No new bone formation was seen in calvarial defects in any group at 4 weeks. Bridging bone formation from the defect margin was marked at 8 weeks in most type I collagen groups. Although immunohistochemical findings with BMP-2, -4, and VEGF were not significantly different, marked vWF immunoreactivity was present. vWF staining was especially strong in endothelial cells in newly formed bone margins in the 100:0, 80:20, and 70:30 ratio type I collagen groups at 8 weeks. The calcium compositions from the elementary analysis were not statistically significant. Many types of artificial bone have been used as bone graft materials, but most of them can only be applied as an inorganic material. This study confirmed improved bony regeneration by adding organic type I collagen to inorganic HA and TCP mixtures. Therefore, these new artificial bone graft materials, which are under strict storage and distribution systems, will be suggested to be available to clinical dentistry demands.

• Microbiology and Biotechnology Letters
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• v.17 no.5
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• pp.468-474
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• 1989

The cultural condition for polysaccharide production by Pseudomonus detafietdii was studied. The optimal medium contains the following composition per liter of distilled water: glucose (25g/$\ell$), peptone (2.06g/$\ell$), KH$_2$PO$_4$(2g/$\ell$), MgSO$_4$.7$H_2O$ (2g/$\ell$), yeast extract (0.5g/$\ell$), CaCO$_3$(2.5g/$\ell$). The temperature and pH optimum were 3$0^{\circ}C$ and 6.5. The agitation speed was 300 rpm. 5.91g of polysaccharide was produced at the condition in flask culture.

• Journal of Sensor Science and Technology
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• v.23 no.1
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• pp.58-65
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• 2014

We developed a low-cost automatic diagnosis system for water quality in cooling towers to measure the concentrations of key ingredients such as $Ca^{2+}$, $Cl^-$, $PO{_4}^{3-}$, and $Fe^{2+}$. $Ca^{2+}$, and $Cl^-$ are the main factors that cause the generation of scale, corrosion, and sludge in water pipes. $PO{_4}^{3-}$ prevents corrosion, sludge and scale by inhibiting the ions (i.e., $Ca^{2+}$, $Cl^-$) from sticking to the pipes. $Fe^{2+}$ is an indicator of pipe corrosion. The proposed system consists of a microprocessor, a specimen container and heater, a precision pump, relays and valves, LED optical sources, and photo detectors. It automatically collects water samples and carries out pretreatment for determining the concentration of each chemical, and then estimates the concentration of each ion using low-cost LED optical sources and detectors. Experimental results showed that the accuracy of the proposed system is sufficiently high for water quality diagnosis and management of cooling towers, demonstrating the possibility of the proposed system's wide usage in real environments.