• 동아시아식생활학회지
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• 제7권1호
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• pp.13-20
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• 1997

This study was designed to determine the effects of protein sources on the curation of gastric ulceration, protein metabolism, and nitrogen balance in rats with gastric ulcer induce by restraint and water immersion stress. After the rats were fed 10% casein diet for 3 weeks, four groups of the rats were forced in 5$\times$5$\times$15cm plexiglass cage. The restraint and water immersion stress was carried at 20$\pm$2$^{\circ}C$ for 8-hour. The other one group(control group) was not exposed to stress. After stress 4 kinds of different diets containing 20% protein were given for 5 days. The protein sources were casein, whey protein, soy protein, gluten. The control group was fed to 10% casein diet. The results were as follows ; the weights of rats were not different among the diet groups During the experiment period follows ; the weights of rats were not different among the diet groups during the experiment period (for 5 days). The ulcer index of rats fed 10% gluten and soy protein diet was significantly higher than those of casein and whey protein diet groups(p<0.05). The level of serum albumin was not significantly different among diet groups. But hematocrit and the level of $\alpha$-amino-N, BU and UUN of plant protein diet groups were higher than animal diet groups, the urinary hydroxyproline of soy protein group was the highest and the whey protein was the lowest. The digestibility and BV of nitrogen of gluten diet group were significantly higher than those of casein and whey protein diet groups(p<0.05). The animal proteins had more curative effects of ulcer than plant animals. The results of this study provide useful information concerning diet therapy for the patients with gastrointestinal diseases and the field of enteral diet materials.

• 한국응용과학기술학회지
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• 제31권1호
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• pp.31-43
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• 2014

This experiment evaluated the interaction effect of extreme heat diet(EHD), inverse lighting, and cool water on the growth performance of broiler chickens under extreme heat stress. There were 4 experimental groups (T1: EHD 1, 10:00-19:00 dark, 19:00-10:00 light, cold water $9^{\circ}C$; T2: EHD 2, 10:00-19:00 dark, 19:00-10:00 light, cold water $9^{\circ}C$; T3: EHD 1, 09:00-18:00 dark, 18:00-09:00 light, cold water $14^{\circ}C$; T4: EHD 2, 09:00-18:00 dark, 18:00-09:00 light, cold water $14^{\circ}C$), each group composed of 25 broilers and the experiment was repeated 3 times. EHD 1 contained soybean oil, molasses, methionine and lysine. EHD 2 contained all nutrients of EHD 1 and vitamin C additionally. As a result, T1 and T2 displayed higher body weight increase and diet intake compared to T3 and T4 (p<0.05). The weights of their liver and gizzard were similar but the weights of the thymus and bursa F were higher for T1 and T2 compared to that of T3 and T4 (p<0.05). It was observed that T1 and T2 displayed higher concentrations of blood triglyceride, total cholesterol, HDL-C and blood sugar compared to that of T3 and T4 but LDL-C level was higher for T3 and T4 compared to that of T1 and T2 (p<0.05). T1 and T2 displayed higher levels of immunity substances such as IgG, IgA and IgM compared to T3 and T4 but the blood level of corticosterone displayed to be lower for T1 and T2 compared to T3 and T4 (p<0.05). The T1 and T2 contained a higher amount of fecal lactobacillus compared to that of T3 and T4 but the T3 and T4 contained a higher amount of fecal E. coli, total aerobic bacteria, coliform bacteria compared to that of T1 and T2 (p<0.05). T1 and T2 displayed higher concentrations of cecal acetic acid, propionic acid and total short chain fatty acids compared to T3 and T4 but T3 and T4 displayed higher concentrations of butyric acid, isobutyric acid, valeric acid and isovaleric acid compared to T1 and T2 (p<0.05). These results have been observed that broiler chickens exposed to extreme heat stress with feeding EHD, inverse lighting and cold water would improve blood lipid, and elevate the production of immunity substance, beneficial microorganisms, and short chain fatty acids. This provision would also reduce the blood sugar consumption rate as energy sources and these effects will improve the growth performance of the broilers exposed to extreme heat.

• Structural Engineering and Mechanics
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• 제45권4호
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• pp.543-568
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• 2013

This study explores the floor micro-vibrations induced by the automated guided vehicles (AGVs) in liquid-crystal-display (LCD) factories. The relationships between moving loads and both the vehicle weights and speeds were constructed by a modified Kanai-Tajimi (MKT) power spectral density (PSD) function whose best-fitting parameters were obtained through a regression analysis by using experimental acceleration responses of a small-scale three-span continuous beam model obtained in the laboratory. The AGV induced floor micro-vibrations under various AGV weights and speeds were then assessed by the proposed regressional MKT model. Simulation results indicate that the maximum floor micro-vibrations of the target LCD factory fall within the VC-B and VC-C levels when AGV moves at a lower speed of 1.0 m/s, while they may exceed the acceptable VC-B level when AGV moves at a higher speed of 1.5 m/s. The simulated floor micro-vibration levels are comparable to those of typical LCD factories induced by AGVs moving normally at a speed between 1.0 m/s and 2.0 m/s. Therefore, the numerical algorithm that integrates a simplified sub-structural multi-span continuous beam model and a proposed regressional MKT moving force model can provide a satisfactory prediction of AGV-induced floor micro-vibrations in LCD factories, if proper parameters of the MKT moving force model are adopted.

• Asian-Australasian Journal of Animal Sciences
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• 제10권2호
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• pp.164-169
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• 1997

The present experiment was conducted to evaluate the effects of feed processing and feeding methods on growth performance and carcass characteristics of growing-finishing pigs. A total of 72 pigs (LYD, 22.24 kg BW) were employed for a 90-d feeding trial. Treatments were 1) mash dry feeding (MD), 2) mash wet feeding (MW), 3) pellet dry feeding (PD), and 4) extruded pellet dry feeding (EPD). Corn, soybean meal and wheat bran in the basal diets were extruded before mixing and pelleting for EPD diet production. Ileal or fecal digestibility and carcass traits including lean meat percentage and weights of stomach ulcer were also examined. During the growing period, pigs fed PD showed improved (p < 0.05) average daily gain (ADG) and feed conversion (F/G) over those fed MD, whereas no significant differences in average daily feed intake (ADFI) were found among dietary treatments. Finisher pigs fed MD showed lover, but not significant, ADG and F/G than those fed MW, PD or EPD. For the overall period, pigs fed PD grew faster (p < 0.05) than those fed MD or EPD. Feed intake was different between the two feeding methods (MD vs MW), and between the two processed feeds (PD vs EPD). The digestibility of crude fat was higher (p < 0.05) in pigs fed EPD than in pigs fed mash feeds. NFE digestibility of EPD treatment was also higher (p < 0.05) than that of PD. Back fat (10th rib area) was thicker (p < 0.05) tn pigs fed MD than in pigs fed EPD. other carcass traits including incidence of esophagogastric ulcers were not different among treatments. In conclusion, pelleting appeared to bo the desirable processing methods and wet feeding could also be recommended for growing-finishing pigs.

• Nutritional Sciences
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• 제8권4호
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• pp.205-211
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• 2005

This study examined the time course effects of conjugated linoleic acid (CLA) on the body weight, adipose depots and lipid profiles of ICR male mice using two different sources of fats in the diet Ninety eight mice weighing 25 to 30 g were divided into four groups: beef tallow (BT) and fish oil (FO), beef tallow with CLA supplementation (BTC), and fish oil with CLA supplementation (FOC) group. Eight to nine mice in each group were fed with the experimental diets for 1, 2 or 4 weeks, respectively. All mice were fed experimental diets containing $12\%$ of total dietary fat (w/w) with or without $0.5\%$ CLA (w/w). CLA supplementation did not affect the body weight The weight of epididymal and visceral fats were significantly lower in BTC compared to those in BT groups during the periods examined (p<0.05), whereas they were significantly lower in FOC than those in FO only at 4 weeks (p<0.05). The levels of triglycerides in the plasma were significantly decreased in the BTC group than in BT group throughout the experimental periods (p<0.05). But, FOC was only effective at 4 weeks as compared to FO. The levels of total cholesterol and HDL-C were significantly increased in the BTC than in BT during the entire period (p<0.05), whereas there were no difference between FO and FOC on the levelsof total cholesterol and HDL-C. The levels of free fatty acids (FFA) were significantly decreased in BTC than in BT at 1 and 4weeks and in FOC only at 4 weeks as compared to FO (p<0.05). Taken these results together, CLA was more effective in the beef tallow diet in lowering the epididymal and visceral fat weights and triglyceride level rather than fish oil diet with CLA. Furthermore, the effect became clearer at 4 weeks than at one week of the experiment.

• Journal of Microbiology and Biotechnology
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• 제9권3호
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• pp.311-317
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• 1999

Human T-cell leukemia virus Type-I (HTLV-I) is etiologically associated with rare adult T-cell leukemia, a malignant T-cell disorder. cDNAs encoding p24 (gag), gp21(env), and pXII of HTLV-I were amplified by polymerase chain reaction (PCR) using the genomic DNA extracted from HUT102 cell line as a template. The amplified cDNAs were cloned into the Escherichia coli expression vectors and over-expression of the recombinant proteins were achieved by adding IPTG into the culture media in order to induce the promoter. The molecular weights of the recombinant p24, gp21, and pXII, determined by SDS-PAGE, were found to be approximately 28 kDa, 23 kDa, and 15 kDa, respectively. Reactivity of the recombinant proteins with human sera was tested by the immunoblot assay. The gp21 and p24 reacted against the sera obtained from HTLV-I-infected individuals but not against the sera obtained from normal persons. These results suggest that the recombinant proteins expressed in E. coli were recognized by antibodies in sera from HTLV-I infected patients. These recombinant proteins would be applicable for detecting the presence of antibodies against HTLV-I in human blood samples.

• 생명과학회지
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• 제23권2호
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• pp.273-281
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• 2013

본 연구는 부산 인근의 해수욕장에서 얻은 해수에서 protease를 생산하는 균주를 분리하여 동정하고 균주의 배양학적인 특성과 protease의 효소학적 특성을 확인하였다. 해수에서 분리한 protease를 생산하는 미생물은 16S rDNA sequencing을 통해 Micrococcus sp. PS-1으로 동정하였다. Protease 생산의 최적조건은 2% skim milk와 1% NaCl이 포함된 pH 7.0의 LB배지에 48시간 배양이었다. 효소의 부분정제를 위해 ultrafiltration과 acetone 침전법을 사용하였고, zymography를 통해 분자량이 35.0 kDa과 37.5 kDa인 protease를 확인하였다. 또한 효소의 최적 활성은 pH 9.0와 $37^{\circ}C$에서 나타났고, 효소는 pH 8.0에서 11.0까지, $25^{\circ}C$에서 $37^{\circ}C$까지 80% 이상의 효소활성이 유지되어 안정한 것으로 확인되었으며 PMSF, EDTA 처리시 protease가 저해되는 것을 통해 alkaline metallo-serine protease로 확인되었다.

• Journal of Nutrition and Health
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• 제40권7호
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• pp.601-605
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• 2007

This study investigated the therapeutic effects of Inonotus obliqua extract on blood glucose, insulin, and other biochemical parameters in genetically diabetic mice $(C57BL/KsJ^-m^{+/+}Lepr^{db})$. The mice were divided into four groups-control, Chaga 1 (dose of 0.09 mg/kg of body weight), Chaga 5 (5 times of Chaga 1), and Chaga 10 (10 times of Chaga 1) - according to supplemented dose. Inonotus obliqua extract was orally administered to the animals for 6 weeks. The body and organ (liver and kidney) weights were not different among groups. Fasting blood glucose level was significantly lower in the Chaga 5 group compared with the control (p < 0.05). Hemoglobin A1c content was significantly lower in the Chaga 5 group compared with either the control and Chaga 1 group (p < 0.05). There was no significant difference in serum insulin level among groups. The glucose-6-phosphatase activity in liver was significantly the lowest in Chaga 10 group and was significantly lower in Chaga 5 group as compared with those of control and Chaga 1 groups. Therefore, the results of this study demonstrate that Inonotus obliqua extract alleviates many of the symptoms of diabetes in genetically obese mice and may offer a possibility as a therapeutic supplement for the normalization of blood glucose levels in human with hyperglycemia and have beneficial effects in patients with non-insulin-dependent diabetes mellitus.

• 생물환경조절학회지
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• 제11권4호
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• pp.199-204
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• 2002

한국의 인삼재배시 연작장해로 인한 생산량 감소가 문제가 되고 있다 이를 극복하기 위하여 본 연구에서는 한국인삼 재배를 위하여 양액재배 시스템을 적용하였다. 최적의 양액재배용 배지를 선발하기 위하여 모래, TSK-2 (S＋T), 피트모스 (P), 재활용 암면 (RR)과 입상 암면 (GR)등의 다양한 배지를 단독 및 혼합처리구로 사용하였다. RR배지의 경우 전반적인 물리화학적 특성은 기존에 보고된 인삼배지의 토양특성과 유사하였다. S＋T배지는 가밀도가 인삼용 토양보다 높았다 인삼의 지상부 생체중은 RR배지와 S＋T배지에서 높았다. 인삼 뿌리의 생체중 및 건물중은 RR배지에서 가장 높았다. 인삼의 뿌리의 품질적 측면에서 vitamin C함량은 양액재배로 생육시킨 인삼이 토양에서 생육한 인삼보다 높았으나, crude saponin과 ginsenosides 함량에서는 차이를 나타내지 않았다.

• 한국가금학회지
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• 제43권4호
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• pp.213-218
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• 2016

본 연구는 고온 스트레스 하에 타우린 첨가가 육계의 간에서 heat shock protein 70(hsp 70) 및 cell free system 상태에서 단백질 합성에 미치는 영향을 조사하였다. 공시동물은 1일령 육계 120수를 2주간 사육 후, 대조(CO, $24^{\circ}C$)와 고온스트레스 처리($34^{\circ}C$)에서 타우린을 공급하지 않은 처리구(HO)와 타우린 0.1%를 공급한 처리구(HT)로 나누어 3처리 4반복, 반복 당 10수씩 배치하였다. 고온 스트레스는 3, 6, 9, 12일간 진행하였다. 최종 체중과 간 무게는 HO와 HT가 CO보다 유의적으로 낮았다(P<0.05). 그러나 타우린을 첨가한 HT은 HO보다 유의적으로 높았다(P<0.05). 육계 간에서 hsp70 발현은 HO가 CO와 HT보다 유의적으로 높았으나(P<0.05), CO와 HT는 유의적인 차이가 없었다. In vitro 실험에서 고온 및 타우린은 21일령 육계 간의 총 단백질 합성률에 영향을 미치지 않았다. 따라서 타우린 섭취가 in vivo 육계의 성장에서 고온 스트레스를 완화시키지만, 생체 내의 단백질 합성에 직접적인 영향을 미치지 않는 것을 보이며, 이러한 결과는 타우린이 다양한 생리학적 기전을 통해 단백질 turnover 대사에 간접적으로 영향을 미칠 수 있다는 것으로 사료된다.