• Journal of Plant Biology
• /
• 제39권3호
• /
• pp.179-184
• /
• 1996

The purpose of this study is to obtain the most efficient combination of Agrobacterium tumefaciens strains and Ti plasmids for the construction of dicotyledonous plant vector system. Ti plasmid-curing A. tumefaciens A136 and KU12C3 were transformed with four kinds of Ti plasmids, pTiBo542, pTiA6, pTiKU12 and pTiAch5, respectively. The stems of 28 species of dicotyledonous plants were then inoculated with these transformants and examined for crown gall formation. The different combination of A. tumefaciens strains and Ti plasmids showed quite a difference in terms of the crown gall formation. Agrobacterium strins A136 and KU12C3 have a same plant host range in case that both strains harour the same kind of Ti plasmid, pTiBo542 or pTiAch5. However, the above-mentioned both strains have quite different host range in the event of containing the same Ti plasmid, pTiKU12 or pTiA6. In case that KU12C3 contains pTiA6 or pTiKU12, this strain has a wider plant host range than A136. The plant host range of pTiBo542 is the widest, followed by pTiA6, pTiKU12 and pTiAch5. Twelve plants among 28 tested plants are not transformed by any virulent Agrobacterium strains used in this study. In conclusion, A. tumefaciens KU12C3 and A136 harboring pTiBo542 showed the widest host range for transforming dicotyledonous plants. Also, it was acertained that the host range of Ti plasmids is affected by chromosomal level.

• The Plant Pathology Journal
• /
• 제24권3호
• /
• pp.269-278
• /
• 2008

Ripe rot was frequently observed on fruits, leaves and stems of grape growing in eight locations in Korea from 2004 to 2006. All 30 isolates of Colletotrichum sp. were obtained from lesions of the ripe rot on grape plants. Out of the isolates, 19 isolates were identified as Colletotrichum acutatum and the others as Colletotrichum gloeosporioides based on morphological and cultural characteristics. Inter and intra specific variations of the Colletotrichum spp. isolates were investigated using RAPD and sequences of rDNA ITS and $\beta$-tubulin-2. Isolates of C. acutatum and C. gloeosporioides were distinctly differentiated by molecular analyses. Phylogenetic trees of ITS and$\beta$-tubulin-2 showed that Korean isolates of C. acutatum were clustered into groups A2 and A3 among the eight global groups. A2 included non-chromogenic isolates and A3 chromogenic ones. Both C. acutatum and C. gloeosporioides isolates were tested for pathogenicity to grape leaves. All isolates tested induced lesions on the leaves of grape by artificial inoculation. There was no difference in pathogenicity between C. acutatum and C. gloeosporioides isolates. This is the first report that C. acutatum except C. gloeosporioides causes grape ripe rot in Korea.

• 한국자원식물학회지
• /
• 제6권2호
• /
• pp.135-140
• /
• 1993

This study was attempted to establish the propagation and cultivation methods of Rhodiola elongata Fisch. et Mey. as a part of development of northern plant resources. Seeds were treated and investingated at the 15, 20, 25$^{\circ}C$ and 100, 200, 400, 800ppm of the growth regulator $GA_3$, BA, and Kinetin. The results obtained are summarized as follows: Seeds of R. elongata were the most vigorously germinated at 15-20$^{\circ}C$, and rate of germination at 25$^{\circ}C$ decreased gradually. Germination rate of seeds that treated growth regulators was remarkably increased than non-treated ones, and $GA_3$ treatment among the growth regulators showed the best effects. The effective concentration of $GA_3$ for germination of R. elongata seeds was 400 ppm.

• 식물분류학회지
• /
• 제50권3호
• /
• pp.253-261
• /
• 2020

Carex splendentissima U. Kang & J. M. Chung, endemic to the Korean peninsula, is characterized by staminate terminal spikes and glabrous elliptic perigynia. Based on its broad leaves, androgynous spikes, and tri-stigmatic features, the species has been placed in Carex sect. Siderostictae Franch. ex Ohwi, an East Asian section and a basal group in the genus. To clarify the monophyly and phylogenetic position of the species, a molecular study using the internal transcribed spacer region of nuclear ribosomal DNA and chloroplast DNA (trnL-F) data was conducted. The DNA sequence data of ten taxa in sect. Siderostictae and closely related taxa (two taxa in sect. Surculosae) with outgroups were analyzed based on maximum parsimony and maximum likelihood (ML) criteria. In the analyses, C. splendentissima was monophyletic and placed within the Siderostictae clade (sect. Siderostictae + two species of sect. Surculosae), forming a clade with C. ciliatomarginata and C. pachygyna (endemic to Japan). The clade (C. splendentissima + C. ciliatomarginata + C. pachygyna) shows evidence of diploidy. Furthermore, C. splendentissima is a sister to C. ciliatomarginata in the ML tree, and the two taxa have staminate terminal spikes. This study also updates the distribution of C. splendentissima and provides keys to the four Korean taxa in sect. Siderostictae. To conserve the endemic species C. splendentissima, further research on its genetic and ecological features should be conducted at the population level.

• 한국산림과학회지
• /
• 제103권3호
• /
• pp.321-338
• /
• 2014

천연기념물 제 432호 제주 상효동의 한란 자생지(39 ha)를 대상으로 2013년 10월부터 2014년 2월까지 생물(식물상, 식생다양성 및 임분 구조) 및 비생물 환경(미기상, 수관열림도, 임내 광량 및 토양 온 습도)의 특성을 분석하였다. 한란 자생지의 일평균온도($5.7^{\circ}C$)는 서귀포시 보다 $3.3^{\circ}C$ 낮았고, 일평균습도(75.8%)는 서귀포시 보다 15% 높았다. 토양 온도는 평균 $16.5^{\circ}C$, 토양습도는 평균 37.3%, 그리고 낙엽층 두께는 평균 4.3 cm이었다. 수관열림도 및 임내 광량의 평균은 각각 15.5%, 및 $8.5mol{\cdot}m^{-2}{\cdot}day^{-1}$ 이었다. 한란 자생지 보호구역 내 관속식물은 총 221 분류군이며, 희귀 및 멸종위기식물로는 한란, 죽백란 및 꼬리족제비고사리 등이 확인되었다. 식생다양성 평가 결과, 구실잣밤나무군락 등 총 6개의 식생단위가 분석되었다. 조사구역의 임분구조는 구실잣밤나무가 가장 높은 밀도(1,777 개체/ha) 및 흉고단면적 ($90.3m^2/ha$)을 나타내었다.

• 한국식물병리학회지
• /
• 제11권1호
• /
• pp.47-52
• /
• 1995

One hundred and thirty bacterial isolates with high chitinolytic activity on chitin agar media were isolated and identified. Most of the isolates were Aeromonas hydrophila (110 isolates), and the others were Serratia marcescens (11 isolates), Aeromonas caviae (3 isolates), Chromobacterium violaceum strain C-61 (2 isolates), Chromobacterium violaceum strain C-72 (1 isolate) and unknown species (3 isolates). Among them, C. violaceum strain C-61 had highest chitinolytic activity and fungal growth inhibition on PDA. This bacterium also inhibited the growth of Rhizoctonia solani, Sclerotinia scelrotiorum, Phytophthora capsici and Pythium ultimum, but it didn't inhibit the growth of Fusarium oxysprum and Fusarium solani. C. violaceum strain C-61 suppressed damping-off of eggplant caused by R. solani. Populations of the chitinolytic bacteria such as Aeromonas hydrophila, Serratia marcescens, Aeromonas caviae, Chromobacterium violaceum strain C-61 and Chromobacterium violaceum strain C-72 introduced into R. solani-infested soil were continuously decreased until 20 days after treatment, but their populations except A. caviae were not changed significantly and maintained over 5$\times$104 CFU per g of soil thereafter.

• 식물병연구
• /
• 제21권3호
• /
• pp.222-226
• /
• 2015

The chitinase-producing bacterial strain C-1 is one of the key chitinase-producing biocontrol agents used for effective bioformulations for biological control. These bioformulations are mixed cultures of various chitinolytic bacteria. However, the precise identification, biocontrol activity, and the underlying mechanisms of the strain C-1 have not been investigated so far. Therefore, we evaluated in planta biocontrol efficacies of C-1 and determined the draft genome sequence of the strain in this study. The bacterial C-1 strain was identified as a novel Serratia sp. by a phylogenic analysis of its 16S rRNA sequence. The Serratia sp. C-1 bacterial cultures showed strong in planta biocontrol efficacies against some major phytopathogenic fungal diseases. The draft genome sequence of Serratia sp. C-1 indicated that the C-1 strain is a novel strain harboring a subset of genes that may be involved in its biocontrol activities.

• 한국자원식물학회지
• /
• 제17권3호
• /
• pp.352-357
• /
• 2004

쓴메밀의 종자의 발아율은 발아온도의 증가와 더불어 증가되는 경향을 보이며, 초장은 3$0^{\circ}C$에서 가장 좋은 생육을 보이고 그 다음으로 $25^{\circ}C$ ＆gt; 2$0^{\circ}C$ ＆gt; 15$^{\circ}C$순으로 온도가 높을수록 초장의 생육이 왕성하였다. 온도처리에 따른 쓴메밀의 초장은 $25^{\circ}C$ ＆gt; 2$0^{\circ}C$ ＆gt; 15$^{\circ}C$ ＆gt; 3$0^{\circ}C$ 순으로 좋았으며, 엽수도 2$0^{\circ}C$ 및 $25^{\circ}C$에서 15$^{\circ}C$ 및 3$0^{\circ}C$보다 다소 증가하는 경향을 보였다. 일장처리에서 16시간 처리구를 제외하면 일장이 길어질수록 초장, 엽수 및 건물 중은 증가하는 경향을 보였으나, 루틴함량과 일장에 대한 일정한 경향은 없었다. 쓴메밀은 초장, 엽수, 분지수 및 경직경 등의 지상부 생육이 단메밀의 생육에 비해 왕성하였다. 특히 엽수에서 단메밀이 62.3에 비해 174.1로 약 2.8배 높았으며, 개체당 종실의 수량은 약 5.4배, 종실무게는 2.1배로 큰 폭의 차이 나타냈다. 쓴메밀 종실의 루틴함량이 1469.8로 단메밀의 22.6보다 약 60배 이상 높게 함유되어 있다.

• The Plant Pathology Journal
• /
• 제26권4호
• /
• pp.372-379
• /
• 2010

Macrophomina phaseolina causing charcoal rot was isolated from sesame seeds (cvs. Giza 32 and Shandawel-3) collected from different localities of Assiut, Sohage and El-Minia Governorates. The fungus was found in the highest frequently in samples collected from Assiut Governorate followed by Sohag and finally EL Minia Governorate. The obtained isolates were different in their virulence on the tested sesame cvs. Also, they differed in their growth nature including colony color and sclerotial production. The color of colonies of the pathogen seem to be correlated with density of sclerotial formation. Aqueous extracts of Majorna, Wild chamomile, Geranium oil and Nees plants were highly toxic to tested isolates of M. phaseolina, in vitro. On the other hand the rest of the tested aqueous extracts had no effect. Under greenhouse conditions in 2005 and 2006 seasons, soaking seeds of sesame before sowing in aqueous extracts of Eucalyptus, Nerium, Ocimum and Roesmary plants decreased the disease incidence. Aqueous extracts of Eucalyptus and Ocimum were the most effective treatment. Dipping sesame seeds in hot water at $60^{\circ}C$ for 5 minutes increased seed germination of Giza 32 and Shandawel-3 cvs. followed by $55^{\circ}C$, $50^{\circ}C$ and $45^{\circ}C$, while $40^{\circ}C$ treatment resulted the lowest seed germination rate. Dipping sesame seeds in hot water at different temperature before planting decreased seed, seedling and charcoal rots. Soaking seeds in hot water at $60^{\circ}C$ increased greatly plant height and decreased seed, seedling rot and charcoal rot followed by $55^{\circ}C$ and $50^{\circ}C$, under greenhouse condition.

• 대한전기학회:학술대회논문집
• /
• 대한전기학회 2008년도 제39회 하계학술대회
• /
• pp.353-354
• /
• 2008

옥외 현장에 설치되어 있는 PANEL은 하절기에 내부 온도가 높아져 PANEL 내부에 설치되어 있는 제어장치의 정상 동작을 방해 할 수 있다. 실제로 하절기 오후 $2{\sim}3$시경 PANEL 내부의 온도는 약 $60{\sim}75^{\circ}C$ 정도의 고온을 유지한다. 근래에는 현장 PANEL 내부에 PLC 및 컨트롤러등 전자화된 제어 장치의 설치 비중이 증가하고 있고 대부분의 컨트롤러 사용 온도는 약 $0{\sim}55^{\circ}C$ 이므로 하절기 고온 상태에서 정상적인 동작을 기대하기는 어렵다. 본 논문에서는 옥외 자립형 PANEL의 냉각 장치를 개발하여 운영하고 그 효과를 분석, 검토함으로써 하절기 고온 상태에서도 안정적으로 설비가 정상 동작하기 위한 방안 제시를 주요 목적으로 한다.