• 한국식물병리학회:학술대회논문집
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• 한국식물병리학회 2003년도 정기총회 및 추계학술발표회
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• pp.143.3-144
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• 2003

A Korean isolate of Pepper mild mottle virus (PMMoV-Kr) was isolated from a diseased pepper crop in Chunchon, Korea. The isolate was biologically purified on Nicoticaa tabacum cv. Xanthi-nc by successive single local transfer steps, and propagated on N. tabacum cv. Samsun. PMMoV-Kr could systemically infect on N. glauca, N. benthmiana, N. occidentalis and Lycopersicon esculentum, which is typical of known isolates of PMMoV. PMMoV-Kr belongs to the pathotype P1,2 based on pepper-tobamoviral indicator experiments; Capsicn chinone harboring L3 gene revealed resistant (necrotic local lesion on inoculated leaf, HR) whereas L+, L1 and L2 pepper plants expressed susceptible reactions of mosaic systemic symptoms for the isolate. To confirm the pathology and delineate symptom determinant of the isolate, full-length cDNAs of PMMoV-Kr were amplified by RT-PCR with a primer set corresponding to the 5'- and 3'-ends of PMMoV. The RT-PCR molecules amplified from genome RNA of the isolate was cloned into the pUC18 vector. Full-length cDNA clones constructed under the control of the T7 RNA promoter could be successfully transcribed to produce in vitro transcript RNA. Infectivity of the capped transcripts and its progeny virus was verified by Western blot and RT-PCR analyses.

• 한국약용작물학회지
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• 제12권5호
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• pp.355-359
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• 2004

A survey was conducted during $April{\sim}May$ 2004 to determine the occurrence and population density of plant-parasitic nematodes in ginseng (Panax ginseng C.A. Meyer) growing fields, in major ginseng growing regions of Chungbuk, Chungnam, Gyeongbuk and Kyongki provinces. The survey revealed presence of eleven species of plant-parasitic nematodes namely, Criconemoides morgensis, Ditylenchus destructor, Helicotylenchus dihystera, Meloidogyne incognita, M. hapla, Paratylencgus lepidus, Pratylenchus penetrans, Psilenchus hilarulus, Trichodorus similis, Tylenchorhynchus claytoni and Xiphinema americanum. Frequency and density of each species were highly variable. M. incognita and M. hapla were the predominant species, their infestation observed in 46.3 and 39.4% fields with an average density of $78{\sim}254\;and\;76{\sim}211$ nematodes per $300\;cm^3$ soil, respectively. Whereas, T. similis and X. americanum were rarely observed; only in 2.3 and 1.8% of surveyed fields and their density was $10{\sim}17\;and\;7{\sim}10$ individuals per $300\;cm^3$ soil, respectively. They are recorded herewith for the first time from ginseng fields of Korea. In nematode-infestated fields, stunted plant growth with chlorotic leaves, and wilted plants were observed in patches.

• KSBB Journal
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• 제15권4호
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• pp.402-404
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• 2000

This work is method that uses a hydrolysis for increasing yield of paclitaxel in plant cell cultures. The best pH is 3.0 to obtain a maximum yield at fixed reaction temperature and time t pH 3.0 reaction temperature 80$^{\circ}C$ and reaction time 8 hr give the highest yield which is three time of control. This is very simple and efficient method to increase paclitaxel yield in plant cell cultures.

• 대한기계학회:학술대회논문집
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• 대한기계학회 2001년도 춘계학술대회논문집D
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• pp.979-984
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• 2001

As a preceding process for developing design technology and establishing operation technology, the design procedure of the SCR(Selective Catalytic Reduction) pilot plant that can handle $1,000Nm^{3}/hr$ of flue gas was reported in this paper. And we also considered several factors that might cause abnormality of the plant in the designing process. The plant was designed and fabricated to test the $DeNO_{x}$ performances in variable operating conditions in the range of $3,000{\sim}36,000hr^{-1}/hr$ in space velocities, $1.67{\sim}6\;m/s$ in linear velocities, $200{\sim}500^{\circ}C$ temperatures, $300{\sim}1,000Nm^{3}/hr$ flow rates, and $0{\sim}1.4:1\;NH_{3}/NO$ ratios. In order to maintain the flow uniformity, the guide vanes and flow straightener were designed and constructed in the plant. The SCR pilot plant can be operated by the automatic control system, which enable to obtain performance data in real time and to set up the operating technology. The catalyst reactor consists of 4 catalyst layers and surface area of each layer can be adjusted to be of small size. Arrangement of catalysts per layer is $3{\times}6$ with the catalyst dimensions of $150{\times}150{\times}500mm(L{\times}W{\times}H)$.

• 대한수의학회지
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• 제58권4호
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• pp.211-217
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• 2018

Mesenchymal stem cells (MSCs) are useful candidates for tissue engineering and cell therapy. Physiological cell environment not only connects cells to each other, but also connects cells to the extracellular matrix that provide mechanical support, thus exposing the entire cell surface and activating signaling pathways. Hydrogel is a polymeric material that swells in water and maintains a distinct 3-dimensional (3D) network structure by cross linking. In this study, we investigated the optimized cellular function for canine adipose tissue-derived MSCs (cAD-MSCs) using hydrogel. We observed that the expression levels of Ki67 and proliferating cell nuclear antigen, which are involved in cell proliferation and stemness, were increased in transwell-hydrogel (3D-TN) compared to the transwell-normal (TN). Also, transforming growth factor-${\beta}1$ and SOX9, which are typical bone morphogenesis-inducing factors, were increased in 3D-TN compared to the TN. Collagen type II alpha 1, which is a chondrocyte-specific marker, was increased in 3D-TN compared to the TN. Osteocalcin, which is a osteocyte-specific marker, was increased in 3D-TN compared to the TN. Collectively, preconditioning cAD-MSCs via 3D culture systems can enhance inherent secretory properties that may improve the potency and efficacy of MSCs-based therapies for bone regeneration process.

• 식물병연구
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• 제26권2호
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• pp.61-71
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• 2020

당근검은잎마름병균인 Alternaria dauci KACC42997에 대해서 32개 살균제를 선발하여 균사생장 억제효과를 조사한 결과, '다2'군, '다5'군, '사1'군, '마2'군, '마3'군에 속하는 살균제의 균사생장 억제효과가 우수하였다. Iminoctadine tris-albesilate를 제외한 '카'군에 속하는 보호살균제와 '다3'군에 속하는 pyraclostrobin은 병원균의 포자발아를 억제하는 효과가 우수하였다. 균사생장 억제효과가 우수했던 숙신산탈수소효소 활성을 저해하는 '다2'군과 탈메틸효소 활성을 저해하는 '사1'군 살균제는 우수한 균사생장 억제효과를 보여주면서도, 포자발아 억제효과는 저조하였다. 하지만 '다5'군에 속하는 fluazinam은 균사생장 억제효과뿐만 아니라 포자발아 억제효과도 우수하였다. 특별히 '다2'군에 속하는 fluxapyroxad를 100 ㎍/ml 처리한 경우, 배지 상에서 포자형성을 47.1% 억제하였다. 구미, 평창, 제주 등에서 분리한 검은잎마름병균 집단의 '사'군, '다'군, '마'군 살균제에 대한 저항성 요인값을 비교하면, 제주 지역 병원균 집단의 저항성 요인값이 가장 낮았다. 평창 지역 균주 집단에서 '마2'군에 속하는 fludioxonil 저항성 균주가 2개 발견되었으며, 그들은 모두 iprodione과 procymidone에 대해서 교차저항성을 보였다.

• 한국자원식물학회지
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• 제31권4호
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• pp.363-371
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• 2018

헐떡이풀은 우리나라에서 울릉도에서만 분포하는 자생식물로 전통적으로 약용으로 쓰여왔으며, 관상식물로의 활용도 기대되는 자원식물이다. 본 연구는 헐떡이풀 종자의 휴면타파와 발아를 위한 조건을 확립하고 종자휴면 유형을 분류하고자 수행되었다. 실험은 헐떡이풀 종자에 저온층적처리($5^{\circ}C$에서 0, 12주)와 고온층적처리($23^{\circ}C$에서 0, 4, 8, 12주 처리 후에 $5^{\circ}C$에서 8주, 다시 $23^{\circ}C$에서 배양함), $GA_3$ (0, 10, 100, 1000 mg/L)처리를 수행하였다. 이렇게 처리한 종자는 무균배지에 소독하여 파종하고 온도 $23^{\circ}C$, 광도 $40{\mu}mo{\ell}{\cdot}m^{-2}{\cdot}s^{-1}$ PPFD, 일장16시간 조건의 배양실에서 배양하였다. 헐떡이풀 종자는 자연상태에서 미숙배인 상태로 탈리되며, 종자 내부로의 수분흡수에 대한 물리적인 장벽은 없었다. 그러나 아무 처리 없이 파종하였을 때 30일이 지나도록 전혀 발아하지 않았다. 따라서 헐떡이풀 종자는 형태적 휴면(MD)과 생리적 휴면(PD)을 가지고 있는 것으로 판단하였다. 저온층적처리(0, 12주) 실험의 최종 발아율은 각각 66.7%, 45.6%로 나타났다. 저온처리는 오히려 발아를 약 3주 정도 지연시켰다. 고온층적처리(0, 4, 8, 12주) 실험에서는 최종 발아율이 각각 13.3%, 24.4%, 20.0%, 51.1%로 나타났다. 헐떡이풀 종자의 배는 상대적 고온에서 발달하였다. $GA_3$ 처리는 종자의 휴면을 극복하고 발아를 촉진하였다. $GA_3$ 처리에서의 최종 발아율은 0, 10, 100, 1000 mg/L 처리구에 대하여 각각 33.3%, 45.0%, 42.5%, 72.5%로 나타났다. 위의 결과를 종합하여 헐떡이풀 종자는 non-deep simple 형태생리적 휴면(MPD)을 가지고 있으며, $GA_3$ 처리를 통해 고온을 대체하고 종자의 휴면을 타파할 수 있었다. 이는 한국에 자생하는 헐떡이풀속의 종자휴면에 대해 처음으로 보고한 논문이다.

• Journal of Plant Biotechnology
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• 제2권3호
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• pp.115-121
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• 2000

A cDNA clone encoding chloroplast triosephosphate isomerase (TPI-cp) was isolated from strawberry fruit cDNA library. Sequence analyses indicated that the cDNA contains an open reading frame of 314 amino acids (33.5 kDa) composed of a transit peptide (59 amino acids) in amino terminal region and mature protein (255 amino acids). The existence of transit peptide in the deduced amino acid sequence implies that it encodes a chloroplast isoform. The protein sequence is more similar to other plant chloroplast isoforms than cytosolic isoforms. RNA blot analysis indicated that its expression is ubiquitous in examined five tissues, flowers, leaves, petioles, roots and fruits, and shows differential pattern according to fruit ripening. Genomic DNA blot analysis showed that TPI-cp is encoded by multiple genes in strawberry. Through sequence comparison and phylogenetic tree construction, TPI-cp is distinctively grouped into dicot and chloroplast isoforms.

• Journal of Plant Biology
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• 제37권2호
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• pp.167-173
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• 1994

Poly(A)+ RNA was selected from Canavalia lineata root nodule RNA through oligo(dT) cellulose column and used for construction of a cDNA library using λgt10-EcoRI arms. The size of the library was 7.2$\times$105 pfu/mL. A full length leghemoglobin (Lb) cDNA clone, pCILb1(687 bp) isolated with soybean Lb probe, contained one open reading frame (ORF) of 447 bp with 54 bp plus 186 bp at 5' and 3' untranslated region, respectively. A consensus sequence of plant translation start region (AAAATGGG) was found at 5' untranslated region, and two polyadenylation-related sequence (AATAAA, AATAAG) and a conserved motif between them (gACTTGTT) were found upstream of poly(A)+ tail consisted of 13 (A)s at 3' untranslated region. The ORF encoded a polypeptide consisted of 149 amino acids with a molecular weight of 16.2 kD. Deduced amino acid sequences showed high degree of homology values with those of other Lbs ranging from 66% (Casuarina glauca) to 85% (Glycine max).

• Journal of Plant Biology
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• 제32권3호
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• pp.145-150
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• 1989

Mature zygotic embryos dissected from ginseng(Panax ginseng C. A. Meyer) seeds were cultured on Murashige and Skoog's (MS) medium containing various concentrations of 2, 4-dichlorophenoxyacetic acid(2, 4-D) and kinetin. Somatic embryos were induced directly from cotyledonary tissue or from intervening callus. The induction frequency of somatic embryos was up to 55%. Upon transfer to half-strength MS medium supplemented with 1 mg/1 6-benzyladenine(BA) and 1 mg/1 GA3, most somatic embryos developed into plantlets. Over 50% of the plantlets flowered after 4 weeks of culture and then a few bore immature fruits in vitro. Therefore, it is suggested that the juvenility of the ginseng tissue which give rise to somatic embryos does not interfere with in vitro flowering of their regenerated plantlets.