• 한국균학회소식:학술대회논문집
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• 2014.10a
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• pp.13-13
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• 2014

Species belonging to the genus Fusarium are widely distributed and cause diseases in many plants. Isolation of fungal strains from air or cereals is necessary for disease forecasting, disease diagnosis, and population genetics [1]. Previously we showed that Fusarium species are resistant to toxoflavin produced by the bacterial rice pathogen Burkholderia glumae while other fungal genera are sensitive to the toxin, resulting in the development of a selective medium for Fusarium species using toxoflavin [2]. In this study, we have tried to elucidate the resistant mechanism of F. graminearum against toxoflavin and interaction between the two pathogens in nature. To test whether B. glumae affects the development of F. graminearum, the wild-type F. graminearum strains were incubated with either the bacterial strain or supernatant of the bacterial culture. Both conditions increased the conidial production five times more than when the fungus was incubated alone. While co-incubation resulted in dramatic increase of conidial production, conidia germination delayed by either the bacterial strain or supernatant. These results suggest that certain factors produced by B. glumae induce conidial production and delay conidial germination in F. graminearum. To identify genes related to toxoflavin resistance in F. graminearum, we screened the transcriptional factor mutant library previously generated in F. graminearum [3] and identified one mutant that is sensitive to toxoflavin. We analyzed transcriptomes of the wild-type strain and the mutant strain under either absence or presence of toxoflavin through RNAseq. Expression level of total genes of 13,820 was measured by reads per kilobase per million mapped reads (RPKM). Under the criteria with more than two-fold changes, 1,440 genes were upregulated and 1,267 genes were down-regulated in wild-type strain than mutant strain in response to toxoflavin treatment. A comparison of gene expression profiling between the wild type and mutant through gene ontology analysis showed that genes related to metabolic process and oxidation-reduction process were highly enriched in the mutant strain. The data analyses will focus on elucidating the resistance mechanism of F. graminearum against toxoflavin and the interaction between the two pathogens in rice. Further evolutionary history will be traced through figuring out the gene function in populations and in other filamentous fungi.

• Journal of Korean Society of Environmental Engineers
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• v.27 no.5
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• pp.491-498
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• 2005

J sewage treatment plant (WWTP) in Busan has used methanol as an external carbon source for the biological denitrification process. Methanol is widely used. but rather expensive and very dangerous in handling. Therefore, it has been required that the economic alternative carbon source must be developed. By-product from a fine chemical industry can be Purified by removing high molecular weight substances using the ultrafilter membrane separation process and RBDCOD fraction becomes $98{\sim}99%$ of COD substances in the purified by-product. The purified by-product containing three types of alcohols, methanol, prophylenglycol and methoxypropanol; showed similar chemical characteristics to the methanol, a main external carbon source, in biodegradation pathway. Shown above, the compatibility between main and alternative carbon sources has been achieved. Also very short or no adaptation period is necessary in the case of exchanging these carbon sources. The compatibility between external carbon sources is an essential element for stabilizing WWTP operations. During the full-scale application test of the by-product, the alternative carbon source line got on par with the treatment efficiency of the methanol line. With the test result, J-WWTP changed methanol to a fine chemical by-product, in two out of three J-WWTP lines. Moreover, it is expected that 55.4% of the external carbon source cost reduction can be achieved in the alternative carbon source applied lines.

• Spatial Information Research
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• v.20 no.3
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• pp.15-25
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• 2012

In order to resolve transportation system focused on vehicles which have led to all sorts of problems such as traffic congestion, air pollution and so on, Korea recently have tended to center around Transit Oriented Development(TOD) which is capable of initiating public traffic demands. It is imperative to develop objective evaluation method which is able to measure pedestrian environment and amenity in order to facilitate green transit. The purpose of this paper is to present evaluation indices and measurement framework of pedestrian environment by analyzing effect on TOD major planning factors such as diversity, density, design, and supply etc. For this, we applied evaluation index with regard to TOD planning factors, investigating connection to pedestrian and employed AHP (Analytic Hierarchy Process) so as to quantify the result of measurement in Jongro 3ga and Hangangjin station. As a result, we presented relationship between travel patterns of pedestrian and each TOD planning factor. More importantly, the proposed framework is expected to make the best of the visualization as well as evaluation method for the pedestrian accessibility, convenience of public transportation, and the mixed land-use patterns in subway area and transit center.

• KSCE Journal of Civil and Environmental Engineering Research
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• v.37 no.4
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• pp.729-738
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• 2017

With the signing of the Paris Agreement, which is the new climate change agreement at the end of 2015, it will have a great impact on Korea environmental policy. The construction industry, which accounts for 42% of Korea's total $CO_2$ emissions, has been implementing various policies to improve the environmental problems. However, it is only applying passively to other projects except eco-friendly building certification. This is because most of the eco-related systems are based on building facilities. Therefore, there is a need for a new eco - friendly design evaluation model that can be widely applied not only to architecture but also to civil engineering facilities. In this study, a new model is developed based on the existing VE model, which adds new factors to evaluate the environmental friendliness, potential environmental pollution concept and environmental risk of facilities. This model is an eco-friendly design evaluation model that enables decision makers to effectively select alternative environmental criteria at the design stage. As a result of the case analysis of the block retaining wall and the alternative retaining wall, the value of the eco - friendly value of the alternative was 1.026 times higher than the original one. If this model is used at the design stage, it is expected to contribute not only to the construction of environmentally friendly facilities but also to the reduction of carbon emissions.

• Journal of Environmental Science International
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• v.22 no.7
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• pp.787-799
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• 2013

The aim of this study was to investigate the accumulation of metallic elements and the control effect of marine pollution caused by ocean dumping in the sediments at a waste disposal area in the Yellow Sea. In July 2009, concentrations of organic matter and metallic elements (Al, Fe, As, Cd, Cr, Co, Hg, Ni, Mn, Pb, and Zn) were measured in surface sediments at the site. The ignition loss (IL) in the surface sediments showed a mean value of 15.4%, about 1.5 times higher than the mean value of the sediments in the coastal areas of Korea. The chemical oxygen demand (COD) at some disposal sites exceeded 20 mg $O_2/g{\cdot}dry$, which signifies the initial concentration of marine sediment pollutants in Japan. The disposal sites contain higher concentrations of Cr, Cu and Zn than the sediments of bays and estuaries that might be contaminated. The magnitude of both metal enrichment factors (EF) and adverse biological effects suggest that pollution with Cr and Ni occurred due to the dumping of waste in the study area. In addition, the geoaccumulation index (Igeo) showed that the surface sediments were moderately contaminated. By the mid-2000s, when the amount of waste dumped at this site was the highest, the concentration of metallic elements was higher than ever recorded. On the other hand, in 2008-09, the need for environmental management was relatively low compare with the peak. As a result, the quality of marine sediment has been enhanced, considering the effect of waste reduction and natural dilution in the disposal area.

• Resources Recycling
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• v.18 no.3
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• pp.11-19
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• 2009

Recently the problem of global environment is became by social issue. Accordingly the interests to recycling and saving of resources are growing from daily life to varieties field of industry. To preserve the global environment, prevent global warming, environmental destruction, environmental pollution by wastes, the drain of aggregate, plasticity energy of cement and decrease in carbon dioxide are an urgent problem that must be resolved. So there is to a field of building industry and stands but on the inside of the building the many double meaning resources usefully, applies. Also the seller masterpiece building where the service life is long planned is safe and comfortably, maintenance, suppresses the construction which is not necessary is unnecessary. Also the seller masterpiece building where the service life is long planned is safe and comfortably, maintenance, suppresses the construction which is not necessary is unnecessary. By revitalizing effective use of limited earth resources, recycling and controling production of construction waste, this study introduced to a method for Long-Term Durability of Construction Structure and Effective Use of Technology for Construction Waste considering architectural demand and earth environment. It is for reduction of an earth environment load from the side of construction production and performance design of a structure.

• Journal of Korea Port Economic Association
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• v.27 no.2
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• pp.297-311
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• 2011

This paper deals with a strategic investment decision model for improving investment profit in shipping industry. Despite the quantitative expansion of Korean shipping business, many shipping firms have suffered financial difficulties due to financial and operating risks that result from the characteristics of capital-intensive business as well as of volatility of shipping markets. As a result, managers in charge of making an investment decision, particularly in tramper business sector, are required to take both financial and operating risk factors into consideration. Put it differently, managers are strongly recommended to avoid these risks by ship asset play; buy-low and sell-high, which results in considerable capital gain and cost reduction. In addition, managers in shipping industry are also recommended to consider the ship chartering investment alternative when the freight markets show extreme volatility as the case of 2008 triggered by sub-prime mortgage financial crisis in USA. For example, the BDI suffered plunging down from 1000 in 2008 to 100 in 2010. Consequently, the 4th largest shipping company in Korea, DAEHAN Shipping Co., has collapsed primarily due to excessive tonnage expansion during the peak time of bulk market. In sum, the strategic investment decision model, suggested in this paper, is designed to include such factors as capital gain by asset play, timely chartering for alternative shipping service, and optimization of operating profit by tonnage adjustment in accordance with change in the shipping markets concerned.

• Clinical and Experimental Reproductive Medicine
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• v.47 no.2
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• pp.114-121
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• 2020

Objective: Despite extensive research on implantation failure, little is known about the molecular mechanisms underlying the crosstalk between the embryo and the maternal endometrium, which is critical for successful pregnancy. Profilin 1 (PFN1), which is expressed both in the embryo and in the endometrial epithelium, acts as a potent regulator of actin polymerization and the cytoskeletal network. In this study, we identified the specific role of endometrial PFN1 during embryo implantation. Methods: Morphological alterations depending on the status of PFN1 expression were assessed in PFN1-depleted or control cells grown on Matrigel-coated cover glass. Day-5 mouse embryos were cocultured with Ishikawa cells. Comparisons of the rates of F-actin formation and embryo attachment were performed by measuring the stability of the attached embryo onto PFN1-depleted or control cells. Results: Depletion of PFN1 in endometrial epithelial cells induced a significant reduction in cell-cell adhesion displaying less formation of colonies and a more circular cell shape. Mouse embryos co-cultured with PFN1-depleted cells failed to form actin cytoskeletal networks, whereas more F-actin formation in the direction of surrounding PFN1-intact endometrial epithelial cells was detected. Furthermore, significantly lower embryo attachment stability was observed in PFN1-depleted cells than in control cells. This may have been due to reduced endometrial receptivity caused by impaired actin cytoskeletal networks associated with PFN1 deficiency. Conclusion: These observations definitively demonstrate an important role of PFN1 in mediating cell-cell adhesion during the initial stage of embryo implantation and suggest a potential therapeutic target or novel biomarker for patients suffering from implantation failure.

• Journal of the Korean Institute of Gas
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• v.20 no.6
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• pp.9-15
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• 2016

Liquefied petroleum gas (LPG) and compressed natural gas (CNG) are often used as fuel for vehicles because they are clean alternative gas fuels. CNG, as a low-carbon fuel, can contribute to the reduction of greenhouse gas emissions. LPG is often used as fuel for taxis because the performance is almost the same as that of gasoline but the price is lower. In the present study, the exhaust gas and the particle number (PN) of particulate matter, which is a recent environmental issue, were compared between LPG and CNG for the same vehicle. A chassis dynamometer was used to conduct the test according to the Federal Test Procedure (FTP)-75 and Worldwide harmonized Light-duty vehicle Test Procedure (WLTC) modes. The PN values of discharged particles having sizes of 5 nm or larger and 23 nm or larger were measured using two condensation particle counters (CPC). The ratio of carbon dioxide was high in the exhaust gas from the LPG vehicle; the ratio of methane was high in the exhaust gas from the CNG vehicle. The PN values of the emitted particles from the two fuels were similar. The PN values of particles having sizes of 23 nm or smaller were high in the high-speed WLTC mode.

• Journal of the Society of Disaster Information
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• v.14 no.3
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• pp.288-295
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• 2018

Purpose: The paper aims to compare the effectiveness of the two types of transport demand management measures, namely pull measures and push measures. Method: Case studies of two metropolitan areas in the developing world assess the extent to which increases in fuel prices can contribute to reducing automobile use and increasing the public transport use and the potential of urban rail transit to cause mode shift from automobiles. Within the case studies, a stated response survey of current car users has been conducted for Cairo and an on-line survey of rail transit users in Algiers. Results: There was a major proportion of car drivers who intend to switch to public transport, depending on the range of fuel prices in Cairo and a considerable proportion of rail users who have switched from automobiles resulting in a measurable reduction in CO2 emissions in Algiers. Conclusion: Investments in urban rail can be highly effective where there are demands for better public transport, but this type of pull measures can be much more effective if combined with push measures which significantly raise driving costs.