• Applied Microscopy
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• v.30 no.4
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• pp.357-365
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• 2000

Paneth cells have been suggested to contribute to the elimination of excess metals into the intestinal lumen. The purpose of this study wat to investigate the changes of the zinc pools in rats subjected to functional loading with zinc salt by mean of both light and electron microscopical autometallography (AMG). Wistar rats 4 were administrated with zinc chloride (20 mg/kg body weight) intraperitoneally dissolved in 1 ml distilled water. The control group received 1 ml saline IP. After further one hour the animals were transcardially perfused with 0.4% sodium sulphide dissolved in 0.1 M PB fellowed by 3% glutaraldehyde solution for 10 minutes. Pieces of ileum were frozen with solid $CO_2$ and sectioned on a cryostat. The sections $(20{\mu}m)$ were autometallographically developed. Sections selected for EM were reembedded on top of a blank Epon block, from which ultrathin sections (100 nm) were cut. The ultrathin sections were double stained with uranyl acetate (30 min) and lead citrate (5 min), then examined under electron microscope. Studies of comparable sections from control and zinc loaded animals with the AMG selenium method gave quite different results. The control animals demonstrated a weakly positive staining in the cytoplasm of the Paneth cells. In the electron microscope the AMG silver grains were found to be located in the cytoplasm, while the electron dense secretary granules and other cell organelles were void of staining. Few AMG grains were located at the apical surface of the Paneth cells. In sections from zinc loaded rats, the AMG grains were seen in abundance in the lumen of the Lieberkuhn crypts at light microscopic levels. At EM levels the zinc revealing silver grains were located in the cytoplasm as in the controls, but much more AMG grains were shifted into the secretary granules. Furthermore, profound AMG grains were found in the lumen of the crypts and surrounding vessels. And a few grains were seen in the endothelium. The AMG technique demonstrated a pattern of AMG grains in the Paneth cells that strongly suggests a transport of zinc ions through these cells.

• Polymer(Korea)
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• v.29 no.6
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• pp.543-548
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• 2005

Double-layered spheres play an important role in controlling drug delivery for pharmaceutical application, because of the low initial burst compared with single-layered spheres and targetable delivery to specific organ. But it has drawback in loading drug and controlling size. In this study, we developed double-layered spheres using relatively simple oil-in-water (O/W) solvent evaporation method witw/without ultrasonication and investigated the size variation of the double-layered microspheres on the contents of poly(lactide- co-glycolide) (PLGA). Double - layered spheres were char-acterized by scanning elecron microscope (SEM), camscope, and confocal fluorescence laser microscope (CFLM). Double-layered spheres showed smooth surfaces and obvious difference between core and corona by SEM observation and camscope. We observed the fluorescent core in the double-walled spheres composed of FlTC-dextran and PLGA using CFLM. It was found that the core of the microsphere was dextran and the corona of the fabricate microsphere was PLGA. Also, the more PLGA concentration, the more the size of the fabricating double-layered sphere observed.

• Journal of the Korea Concrete Institute
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• v.23 no.5
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• pp.617-626
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• 2011

Seven post-tensioned lightweight concrete (LWC) beam specimens were tested under a symmetrical two-point top loading system. The parameters investigated were the amounts of mild longitudinal reinforcement and effective prestressing. The design compressive strength and dry density of the LWC tested were 30 MPa and 1,770 $kg/m^3$, respectively. Similar to post-tensioned normal weight concrete (NWC) beams, the crack propagation and stress increase of the unbonded tendons were significantly affected by the amounts of mild longitudinal reinforcement and effective prestressing. With the increase in the amounts of mild longitudinal reinforcement and effective prestressing, the serviceability and flexural capacity of the beams were enhanced whereas the stress increase in the unbonded tendons decreased. To control the crack width in post-tensioned LWC beams, a minimum amount of mild longitudinal reinforcement specified in ACI 318-08 provision is required. The flexural behavior of post-tensioned LWC beams and stress increase of the unbonded tendons could be rationally predicted by the proposed non-linear two-dimensional analysis. On the other hand, ACI 318-08 flexure provision was too conservative about the post-tensioned LWC beams.

• Journal of Korean Society of Steel Construction
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• v.29 no.3
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• pp.249-260
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• 2017

In order to investigate the structural performance of a novel prefabricated-SRC column using bolt-connected steel angles(PSRC column), eccentric axial loading tests were performed for six PSRC column specimens and two conventional SRC column specimens. The test parameters were the spacing and sectional configurations of lateral reinforcement, and eccentricity ratio of axial load. The test results showed that, due to high axial-stiffness of the angles located at the corners of the cross section, the compressive load-carrying capacity and deformation capacity of the PSRC specimens were greater than those of the SRC specimens in the large eccentricity ratio of axial load. Closely spaced lateral steel plates and Z-shaped lateral steel plates improved lateral confinement, which increased the load-carrying capacity of the PSRC specimens. The combined flexural and axial load-carrying capacity of the specimens by tests and nonlinear numerical analysis were greater than the predictions by current design codes. The numerical analysis agreed well with the test results including the initial stiffness, peak strength, and post-peak strength degradation.

• KSBB Journal
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• v.14 no.6
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• pp.688-695
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• 1999

Biodegradation of the reactive dye, Remazol Black B was investigated in an upflow anaerobic sludge blanket(UASB) reactor. Important parameters studied include dye concentration(20-60 mg/L), glucose concentration as a co-substrate(1,000-3,000 mg/L), hydraulic retention time(3-24 hr), and influent pH(6.0-8.0). Under most conditions tested, the molecules of Black B were degraded readily and completely according to HPLC chromatograms. However, the color removal efficiency based on spectroscopic measurement was always approximately 75%. This suggests that the degradation products have some color intensity corresponding to 25% of the original dye molecules. The maximum influent dye concentration which satisfies the legal discharge limit of color intensity of 400 ADMI was 13 mg/L. and the highest removal rate at this dye concentration was 104 mg/L${\cdot}$day.

• Korean Chemical Engineering Research
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• v.47 no.1
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• pp.89-95
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• 2009

Because of high contents of cellulose (~37 wt%) and hemicellulose (~17%), rice straw seems to be a potential lignocellulosic biomass for production of bioethanol. In this study, Ammonia Recycled Percolation (ARP) pretreatment of rice straw was extensively investigated. In particular, the experimental study included the effects of temperature, reaction time and concentration of ammonia on compositions and enzymatic digestibility of the resulting solid residues; the ranges of pretreatment conditions were, in turn, $150{\sim}190^{\circ}C$, 10~90 min and 0~20 wt%. Through ARP pretreatment, the lignin content was reduced by as high as ~84% while 20~80% of the hemicellulose was also solubilized. The solid residue resulted from the pretreatment with 15 wt% aqueous ammonia solution at $170^{\circ}C$ for 90 mim showed as high as ~90% of digestibility with 15FPU/g of glucan enzyme loading. Supplement of xylanese to cellulase led to a notable enhancement of digestibility, indicating a discernable inhibitory role of hemicellulose. Simultaneous Saccharification and Fermentation (SSF) and Simultaneous Saccharification and Co-Fermentation (SSCF) were performed to obtain ethanol productions of 13.8 g/L (corresponding to 81% yield) and 15 g/L (corresponding to 89% yield), respectively.

• Journal of the Korean Geotechnical Society
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• v.34 no.7
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• pp.39-49
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• 2018

Jacked pile that involves the use of hydraulic jacks to press the piles into the ground is free from noise and vibration, and is possibly installed within a limited construction area. Thus, as an alternative to conventional pile driving methods, pile jacking could become widely accepted for the construction projects in urban area (e.g., reconstruction or remodeling construction projects). Great concern has arisen over the prediction of axially loaded jacked pile behavior. Against this background, a series of pile load tests were hence conducted on a jacked steel pipe pile installed in weathered zone (i.e., weathered soil and weathered rock). From the test results, base resistance and shaft resistance for each test condition were evaluated and compared with the values predicted by the previous driven pile resistance assessment method. Test results showed that the previous driven pile resistance assessment method highly underestimated both the base and shaft resistances of a jacked pile; differences were more obviously observed with the shaft resistance. The reason for this discrepancy is that a driven pile normally experiences a larger number of loading/unloading cycles during installation, and therefore shows significantly degraded stiffness of surrounding soil. Based on the results of the pile load tests, particular attention was given to the modification of the previous driven pile resistance assessment method for investigating the axially loaded jacked pile behavior.

• Korean Journal of Acupuncture
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• v.18 no.1
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• pp.157-164
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• 2001

Radix Asteris has been used in Korea for many centuries as a treatment for respiratory disease. The effect of Radix Asteris on tracheal smooth muscle is not known. The purpose of the present study is to determine the effect of Radix Asteris on histamine induced tracheal smooth muscle contraction in rats and guinea pigs. Guinea pig(500g, male) and Sprague Dawley rats (250g, male) were killed by $CO_2$ exposure and a segment (8-10mm) of the thoracic trachea from each rat and guinea pig was cut into equal segments and mounted 'in pairs' in a tissue bath. Contractile force was measured with force displacement transducers under 0.5g loading tension. The dose of histamine (His) which evoked 50% of maximal response ($ED_{50}$) was obtained from cumulative dose response curves for histamine $(10^{-7}{\sim}10^{-4}M)$. Contractions evoked by His ($ED_{50}$) were inhibited significantly by Radix Asteris. In guinea pig tracheal smooth muscle, the mean percent inhibition of histamine induced contraction was 120.5% (p<0.01) after $100{\mu}l/ml$ Radix Asteris. In rat tracheal smooth muscle, the mean percent inhibition of histamine induced contraction was 135.4% (p<0.01) after $100{\mu}l/ml$ Radix Asteris. Propranolol $(10^{-7}M)$ slightly but significantly attenuated the inhibitory effects of Radix Asteris. Following treatment with propranolol, the mean percent inhibition caused by $100{\mu}l/ml$ Radix Asteris fell to 44.6% in guinea pig induced by histamine contraction and by $100{\mu}l/ml$ Radix Asteris fell to 18.7% (p<0.05) in rat induced by histamine contraction. Indomethacin and methylene blue$(10^{-7}M)$ did not significantly alter the inhibitory effect of Radix Asteris. These results indicate that Radix Asteris can relax histamine induced contraction of guinea pig and rat tracheal smooth muscle, and that this inhibition involves sympathetic effects.

• Food Science and Preservation
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• v.24 no.7
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• pp.915-922
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• 2017

This study aimed in onion production by evaluating cost and labor efficiencies of onion storage methods using either a ton-bag or a wire-steel pallet. New methods using ton-bag and wire-steel pallets were developed and applied to postharvest tasks, such as harvest packaging, transportation, and storage. The storage parameters evaluated for their effect on the logistics of onion production were: working duration, working hours, and cost expenditure. The longitudinal tensile strength of the ton-bag developed in this study was 16% higher than that of the conventional ton-bag. The wire-steel pallet developed in this study had 10% more storage capacity in a low-temperature storage room, and its truck loading capacity was more than doubled compared to that of the conventional steel pallet. There was no difference in the wasting rate during bulk storage between the newly developed wire-steel pallet and the conventional steel pallet, for 500 kg of onions. However, the bulk storage of 1,000 kg of onions using the wire-steel pallet was not found to be suitable, because the wasting rate of onions stored using the wire-pallet was 3.7% higher than that of onions stored using a conventional steel pallet. The time and the total investing costs for the bulk method decreased by 50.1% and 46.1%, respectively, compared to those for conventional harvest. In the bulk storage using the wire-steel pallet, the total storage cost decreased by 28.8%. Thus, it is estimated that we could have saved 18.3 billion won if the wire-steel pallet method about 30% of the total onion production (1,298,749 M/T) in 2016.

• Restorative Dentistry and Endodontics
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• v.29 no.6
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• pp.548-552
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• 2004

Intracanal disinfection of infected root canal is one of important treatment procedure. This in vitro study aimed to evaluate whether the surface polymers of controlled release drug (CRD) can effectively control the release rate of chlorhexidine for root canal disinfection. Four CRD prototypes were prepared: Group A (n=12); The core device (absorbent paper point) was loaded with 40% CHX solution as control. Group B (n=12); same as group A, but the device was coated with chitosan. Group C (n=12); same as group A and then coated three times with 5% PMMA. Group D (n=12); same as group A and then coated three times with 3% PLGA. All CRD prototypes were soaked in 3 mL distilled water for experimental periods and the concentrations of released CHX from each CRD prototype were determined using a UV spectrophotometer. Results showed that release rate of CHX were the greatest in the non-coated group (control group), followed by the chitosan-coated group, the PLGA-coated group, and the PMMA-coated group (P < 0.05). This data indicate that surface polymers can control the release rate of CHX from the CRD prototypes.